Sathasivam A.,Muthaiyah Research Foundation |
Muthuselvam M.,Marudupandiyar College |
Rajendran R.,Muthaiyah Research Foundation
Global Journal of Pharmacology | Year: 2010
From the ancient period cow's urine has been used as a medicine. In India, drinking of cow urine has been practiced for thousands of years. Panchagavya is a term used in Ayurveda to describe five important substances obtained from cow namely Urine, Dung, Milk, Ghee and Curd. The present study analyzes the antibacterial and antifungal activity of Cow Urine Distillate against the clinical pathogenic microorganisms. Antibacterial activity of Cow Urine Distillate (5, 10 and 15μl) was analyzed against the Bacillus subtilis, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella typhi. Maximum antibacterial activity was observed in Pseudomonas aeruginosa (7.06±0.05, 8.08±0.18 and 10.4±1.23, mm in diameter, respectively) and Salmonella typhi (6.3±1.23, 8.06±0.17 and 10.4±1.2, mm in diameter, respectively). Antifungal activity of cow urine distillate was analysed against Aspergillus niger and Aspergillus flavus. When the two fungal organisms were compared, maximum growth suppression was observed in Aspergillus niger (3±0.14, 6.3±1.2 and 7.06±0.04, mm in diameter) than Aspergillus flavus (2.03±0.25, 4.9±0.26 and 6.3±1.2, mm in diameter, respectively). Finally concluded that the cow urine distillate has antibacterial and antifungal activities and the inhibitory activity can be used in the control of bacteria and fungi of various origins. © IDOSI Publications, 2010.
Govindaraj S.,Marudupandiyar College |
Renganathan S.,Marudupandiyar College
Journal of Pure and Applied Microbiology | Year: 2017
Pleurotus sp is an edible mushroom, which contains essential nutrients and medicinal properties for living thing. Molecular characterization and genome differentiation of pleurotus sp. studied using gel electrophoresis, RAPD and PCR techniques. Separated DNA was amplified using internal transcribed spacer primers. 5.8s rRNA sequence submitted to Genbank for comparison and to obtain accession number. Differentiation and identification of Pleurotus sp found by similarity searches (Accession number: JQ740170 and JQ740171). Comparative studies have shown these two Pleurotus sp (spp1 & ssp2) are closely related to species, 95% -100% identical, Pleurotus Citrinopileatus, Pleurotus populinus, Pleurotus ostreatus, Pleurotus abieticola, Pleurotus pulmonarius, Pleurotus eryngii and Pleurotus sapidus. These Pleurotus sp have been studied for exhibiting pharmacological potential activities such as antioxidant, antitumor, immunomodulatory activity and hypocholesterolemic through sequence similarity, nucleotide composition, evolutionary studies, secondary structure prediction and conserved region. The study extended to identify the significance of species diversity and fungal samples among various applications. Pleurotus (sp) sample was subjected to identification of bioactive compounds by using GC-MS. In Pleurotus spp1: Pyridine-3-carboxamide, 4-dimethylamino-N-(2, 4-difluorophenyl), Piperidin-4-carboxylic acid, Aspidofractinine-3-methanol, (2à, 3á, 5à), Indolizine, and 2-(4-methylphenyl)-. Pleurotus spp2: Imidazolidine, 1, 3-dinitro, Phenol, 2-methyl-4-(1, 1, 3, 3-tetramethylbutyl), and Squalene. Enhancement of more entries as well as sequence comparison and virtual screening might help researchers to acknowledge real world problems between cultivating nutritional, medicinal and poisonous mushroom.
Upgade A.,PRIST University |
Prabakaran P.,Marudupandiyar College
Journal of Pharmaceutical Sciences and Research | Year: 2015
Multidrug resistance in India is direct threat to developing medical technology and sciences. Disease management is somehow tedious task for physicians. Urinary tract infection is one among them; improper diagnosis, unclear symptoms, and resistance due to indiscriminate use of antibiotics are the major reasons. This study designed to develop a combinational therapy of nanotechnology and plant metabolites together for better alternative to traditional medicines. Silver nanoparticles were synthesized using leaves of Carica papaya. Synthesized silver nanoparticles were then subjected to check antibacterial activity against clinical isolates i.e. uropathogens like P. aeruginosa, Klebsiella pneumoniae and E.coli with methanolic plant extract of leaves of Carica papaya. Traditional testing were performed with characterization of silver nanoparticles and results shows it can be prove as a promising therapy in future. As compare to traditional antibiotics herbal drug with nanoparticles have more effective bacteriostatic activity. © 2009 PharmaInfo Publications. All rights reserved.
Shibula K.,Marudupandiyar College |
Velavan S.,Marudupandiyar College
International Journal of Pharmacognosy and Phytochemical Research | Year: 2015
The aim of this study was to carry out for identification of bioactive compounds from the methanolic extract of Annona muricata leaves by Gas chromatography and Mass spectroscopy (GC-MS). GCMS analysis of methanolic extract was done by standard protocol using the equipment Perkin-Elmer Gas Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis revealed the presence of various compounds like 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6, Tetradecanoic acid, 3,7,11,15-Tetramethyl-2-hexadecen-1-ol, Hexadecanoic acid methyl ester, n-Hexadecanoic acid, Phytol and Octadecanoic acid in the methanolic extract of Annona muricata. These findings support the traditional use of Annona muricata in various disorders. © 2015, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.
PubMed | National Institute of Development Administration, Korea University, Korea Institute of Science and Technology, Tohoku University and 3 more.
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2016
Coumaric acid (CA) is a phenolic acid of the hydroxycinnamic acid family, and it has many biological functions such as anti-oxidant, anti-inflammatory, antidiabetic, anti-ulcer, anti-platelet, anti-cancer activities, etc. In the present study, we planned to analyse the potential molecular function of CA on skeletal muscle and preadipocytes differentiation using PCR and Western blot techniques. First, we analysed the impact of CA on C2C12 skeletal muscle differentiation. It revealed that CA treatment inhibited horse serum-induced skeletal muscle differentiation as evidenced by the decreased expression of early myogenic differentiation markers such as Myogenin and myoD via the AMP activated protein kinase- alpha AMPK- mediated pathway. Furthermore, the level of lipid accumulation and changes in genes and protein expressions that are associated with lipogenesis and lipolysis were analyzed in 3T3-L1 cells. The Oil Red O staining evidenced that CA treatment inhibited lipid accumulation at the concentration of 0.1 and 0.2 mM. Furthermore, coumaric acid treatment decreased the expression of main transcriptional factors such as CCAAT/enhancer binding protein-alpha (C/EBP-) and peroxisome proliferator-activated receptor gamma-2 (PPAR-2). Subsequently, CA treatment decreased the expression of sterol regulatory element binding protein-1 (SREBP-1), fatty acid synthase (FAS), acetyl CoA carboxylase (ACC) and adiponectin. Finally, we identified conformational changes induced by CA in PPAR-2 using computational biology tools. It revealed that CA might downregulate the PPAR-2 expression by directly binding with amino acids of PPAR-2 by hydrogen at 3.26 distance and hydrophobic interactions at 3.90 contact distances. These data indicated that CA suppressed skeletal muscle and preadipocytes differentiation through downregulation of the main transcriptional factors and their downstream targets.
Thiruvengadam M.,National Chung Hsing University |
Thiruvengadam M.,Marudupandiyar College |
Rekha K.T.,National Chung Hsing University |
Yang C.-H.,National Chung Hsing University |
And 2 more authors.
Plant Biotechnology Reports | Year: 2010
An efficient protocol for in vitro organogenesis was achieved from callus-derived immature and mature leaf explants of Momordica charantia, a very important vegetable and medicinal plant. Calluses were induced from immature leaf explants excised from in vitro (15-day-old seedlings) mature leaf explants of vivo plants (45 days old). The explants were grown on Murashige and Skoog (MS) medium with Gamborg (B5) vitamins containing 30 g l-1 sucrose, 2.2 g l-1 Gelrite, and 7.7 μM naphthalene acetic acid (NAA) with 2.2 μM thidiazuron (TDZ). Regeneration of adventitious shoots from callus (30-40 shoots per explant) was achieved on MS medium containing 5.5 μM TDZ, 2.2 μM NAA, and 3.3 μM silver nitrate (AgNO3). The shoots (1.0 cm length) were excised from callus and elongated in MS medium fortified with 3.5 μM gibberellic acid (GA3). The elongated shoots were rooted in MS medium supplemented with 4.0 μM indole 3-butyric acid (IBA). Rooted plants were acclimatized in the greenhouse and subsequently established in soil with a survival rate of 90%. This protocol yielded an average of 40 plants per leaf explant with a culture period of 98 days. © 2010 Korean Society for Plant Biotechnology and Springer.
Nandhini R.,SASTRA University |
Jeyadoss T.,SASTRA University |
Velavan S.,Marudupandiyar College
International Journal of Pharma and Bio Sciences | Year: 2014
Liver plays a major role in detoxification. Any impairment to it or dysfunction may lead to many complication on one's health. Treatment of liver diseases is still a challenge to modern medicine. The commerecially available medicine has little to offer for the alleviation of hepatic ailments but sever side effects whereas the most important representatives are of phytoconstituents. The study was aimed to evaluate in vitro hepatoprotective activity of Aplotaxis auriculata rhizome (200 and 400μg/ml) through CCl4 induced toxicity in hepatocytes. The phytochemical screening revealed the presence of flavonoids, terpenoids, triterpenoids, polyphenol and tannins. All the variables tested as LPO, GSH, Protein, Bilirubin, ALP, SGOT and SGPT recorded a significant alteration observed in CCl4 exposed hepatocytes. However treatment with Aplotaxis auriculata extract restored the level and to near normal values was observed. The potential hepatoprotective activity of Aplotaxis auriculata is due to the presence of phytochemical constitution present in plant. Some of these phytochemicals such as flavonoids and polyphenolic compounds might have possessed hepatoprotective activity.
Senthamil Selvan P.,Marudupandiyar College |
Velavan S.,Marudupandiyar College
Rasayan Journal of Chemistry | Year: 2015
The aim of this study was to investigate the bioactive compounds from methanolic extract of Cissus vitiginea leavesby Gas chromatography and Mass spectroscopy (GC-MS). GC-MS analysis of methanolic extract was done bystandard protocol using the equipment Perkin-Elmer Gas Chromatography–Mass Spectrometry, while the massspectra of the compounds found in the extract was matched with the National Institute of Standards and Technology(NIST) library. The GC-MS analysis revealed the presence of various compounds like Tetradecanoic acid (19.658),3,7,11,15-Tetramethyl-2-hexadecen-1-ol (20.921), 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (21.144), Hexadecanoicacid, methyl ester (21.636), Oleic Acid (21.865), l-(+)-Ascorbic acid 2,6-dihexadecanoate(22.057), 9-Octadecenoicacid (22.712), Andrographolide (22.947), Heptadecanoic acid (23.106), Octadecanoic Acid, methyl ester (23.817),9,12-Octadecadienoic acid (24.552) and 22-Tricosenoic acid (26.836) in the methanolic extract of Cissus vitiginea.These findings support the traditional use of Cissus vitiginea in various disorders. ©2015 RASĀYAN. All rights reserved
Upgade A.,Center for Research and Development University |
Prabakaran P.,Marudupandiyar College
International Journal of Pharmacy and Technology | Year: 2015
Urinary tract infections are the widest spread illness in India. Poor understanding and limited diagnosis is the major cause of the infection. This can be divided in two sub categories, such as complicated and uncomplicated infections. Most of this are bacterial and fungal, their cause is due to easy access to soft tissues. If a person is immunocompromised and hospitalised the chances of this infection is more than 70%. Due to lack of knowledge and less focus there is limited therapeutics available. High dose of broad spectrum antibiotics also now a day’s getting resistance. Targeted therapy and natural drug selection is need of a hour. To achieve and develop new arena this pilot study was designed. Specific targets were selected from designated common occurring uropathogens namely Pseudomonas aeruginosa, Klebsiella Pneumoniae and Escherichia coli. Various active compounds were selected from plant leaves of C.papaya exibiting antimicrobial activity. Molecular docking studies were performed using Autodock 4.0 bioinformatics tool. All the compounds were screened for drug likeness activity as well as ADME analysis using bioinformatics software’s. All the ccompounds have been screened and docked for binding energy analysis, the potential docked structures are then recommended for further studies. © 2015, International Journal Of Pharmacy and Technology. All rights reserved.
Mahadevan K.,Tamil University |
Velavan S.,Marudupandiyar College
International Journal of Pharma and Bio Sciences | Year: 2013
Saliva is increasingly used and well validated in diagnosing, monitoring health and disease status, mainly due to its origin, composition, functions, and interactions with other organ systems. The effect of nutrition status on saliva covers a broad spectrum of factors. This includes the degree of malnutrition, the period when malnutrition takes place, the consistency of the diet, the effect on salivary gland growth and function, and the effects on the different salivary glands. Protein-energy malnutrition (PEM) is a serious nutritional problem in slum children's and elder. Serum albumin, globulin, transferring, total protein and amylase activity have been used as biochemical indicators of protein nutritional status and salivary gland functions, but taking blood from the slum subjects are difficult and invasive. We therefore assessed the possibility of using saliva as noninvasive materials to estimate concentrations of albumin, transferrin, total protein and amylase activity. Saliva is collected from 155 slum and healthy subjects of different ages and control (aged 8-15, 24-32, 58-77 yrs) and the concentrations of albumin, transferrin, total protein and amylase activity in saliva were evaluated. Altered levels of protein profile were observed in this study. This result suggested that nutritional deficiencies compromise salivary gland function, thus increasing susceptibility to dental caries and oral infections and reducing quality of life.