Ilavenil S.,South Korean National Institute of Animal Science |
Da Kim H.,Tohoku University |
Srigopalram S.,South Korean National Institute of Animal Science |
Arasu M.V.,King Saud University |
And 5 more authors.
Molecules | Year: 2016
Coumaric acid (CA) is a phenolic acid of the hydroxycinnamic acid family, and it has many biological functions such as anti-oxidant, anti-inflammatory, antidiabetic, anti-ulcer, anti-platelet, anti-cancer activities, etc. In the present study, we planned to analyse the potential molecular function of CA on skeletal muscle and preadipocytes differentiation using PCR and Western blot techniques. First, we analysed the impact of CA on C2C12 skeletal muscle differentiation. It revealed that CA treatment inhibited horse serum-induced skeletal muscle differentiation as evidenced by the decreased expression of early myogenic differentiation markers such as Myogenin and myoD via the AMP activated protein kinase-alpha AMPK-α mediated pathway. Furthermore, the level of lipid accumulation and changes in genes and protein expressions that are associated with lipogenesis and lipolysis were analyzed in 3T3-L1 cells. The Oil Red O staining evidenced that CA treatment inhibited lipid accumulation at the concentration of 0.1 and 0.2 mM. Furthermore, coumaric acid treatment decreased the expression of main transcriptional factors such as CCAAT/enhancer binding protein-alpha (C/EBP-α) and peroxisome proliferator-activated receptor gamma-2 (PPAR-γ2). Subsequently, CA treatment decreased the expression of sterol regulatory element binding protein-1 (SREBP-1), fatty acid synthase (FAS), acetyl CoA carboxylase (ACC) and adiponectin. Finally, we identified conformational changes induced by CA in PPAR-γ2 using computational biology tools. It revealed that CA might downregulate the PPAR-γ2 expression by directly binding with amino acids of PPAR-γ2 by hydrogen at 3.26 distance and hydrophobic interactions at 3.90 contact distances. These data indicated that CA suppressed skeletal muscle and preadipocytes differentiation through downregulation of the main transcriptional factors and their downstream targets. © 2016 by the authors; licensee MDPI, Basel, Switzerland.
PubMed | National Institute of Development Administration, Korea University, Korea Institute of Science and Technology, Tohoku University and 3 more.
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2016
Coumaric acid (CA) is a phenolic acid of the hydroxycinnamic acid family, and it has many biological functions such as anti-oxidant, anti-inflammatory, antidiabetic, anti-ulcer, anti-platelet, anti-cancer activities, etc. In the present study, we planned to analyse the potential molecular function of CA on skeletal muscle and preadipocytes differentiation using PCR and Western blot techniques. First, we analysed the impact of CA on C2C12 skeletal muscle differentiation. It revealed that CA treatment inhibited horse serum-induced skeletal muscle differentiation as evidenced by the decreased expression of early myogenic differentiation markers such as Myogenin and myoD via the AMP activated protein kinase- alpha AMPK- mediated pathway. Furthermore, the level of lipid accumulation and changes in genes and protein expressions that are associated with lipogenesis and lipolysis were analyzed in 3T3-L1 cells. The Oil Red O staining evidenced that CA treatment inhibited lipid accumulation at the concentration of 0.1 and 0.2 mM. Furthermore, coumaric acid treatment decreased the expression of main transcriptional factors such as CCAAT/enhancer binding protein-alpha (C/EBP-) and peroxisome proliferator-activated receptor gamma-2 (PPAR-2). Subsequently, CA treatment decreased the expression of sterol regulatory element binding protein-1 (SREBP-1), fatty acid synthase (FAS), acetyl CoA carboxylase (ACC) and adiponectin. Finally, we identified conformational changes induced by CA in PPAR-2 using computational biology tools. It revealed that CA might downregulate the PPAR-2 expression by directly binding with amino acids of PPAR-2 by hydrogen at 3.26 distance and hydrophobic interactions at 3.90 contact distances. These data indicated that CA suppressed skeletal muscle and preadipocytes differentiation through downregulation of the main transcriptional factors and their downstream targets.
Upgade A.,PRIST University |
Upgade A.,Marudupandiyar College |
Prabhakaran P.,PRIST University |
Prabhakaran P.,Marudupandiyar College
Research Journal of Pharmacy and Technology | Year: 2015
Emergence of specific infections associated with hospitals is special concern as per the recent reviews. As far as, there is uncontrolled population growth responsible for such a conditions. Managing diseases in tertiary care hospital including Government hospital, Private hospitals, mother care homes etc is tedious task. Nosocomial infections caused by the gram negative organisms to the admitted patients of all group occurs. Urine tract infection is one of the most threaten condition those admitted not only that they are multidrug resistant bugs which can difficult to manage. Hence, this study involves medicinally important plant to evaluate the antibacterial effect of plant on selected UTI causing bacteria like Pseudomonas aeruginosa, Klebsiella pneumoniae and E.coli. Traditional methods have been employed to check activity of methanolic extract C. papaya. The multi benefit plant in ancient literature describes about seeds and fruit effectively hence leaves has taken into accounts to accelerate scope of research. In results, the extract shows different antibacterial activity which was confirmed by measuring zone of inhibition as compare to standard antibiotic. E.coli and K. pneumonia was found most sensitive against plant extract and found more effective than antibiotic like Cef: Cefpodoxime, Chlor: Chloramphenicol, Ceftri: Ceftriaxone i.e. 27mm and 30 mm © RJPT All right reserved.
Thiruvengadam M.,National Chung Hsing University |
Thiruvengadam M.,Marudupandiyar College |
Rekha K.T.,National Chung Hsing University |
Yang C.-H.,National Chung Hsing University |
And 2 more authors.
Plant Biotechnology Reports | Year: 2010
An efficient protocol for in vitro organogenesis was achieved from callus-derived immature and mature leaf explants of Momordica charantia, a very important vegetable and medicinal plant. Calluses were induced from immature leaf explants excised from in vitro (15-day-old seedlings) mature leaf explants of vivo plants (45 days old). The explants were grown on Murashige and Skoog (MS) medium with Gamborg (B5) vitamins containing 30 g l-1 sucrose, 2.2 g l-1 Gelrite, and 7.7 μM naphthalene acetic acid (NAA) with 2.2 μM thidiazuron (TDZ). Regeneration of adventitious shoots from callus (30-40 shoots per explant) was achieved on MS medium containing 5.5 μM TDZ, 2.2 μM NAA, and 3.3 μM silver nitrate (AgNO3). The shoots (1.0 cm length) were excised from callus and elongated in MS medium fortified with 3.5 μM gibberellic acid (GA3). The elongated shoots were rooted in MS medium supplemented with 4.0 μM indole 3-butyric acid (IBA). Rooted plants were acclimatized in the greenhouse and subsequently established in soil with a survival rate of 90%. This protocol yielded an average of 40 plants per leaf explant with a culture period of 98 days. © 2010 Korean Society for Plant Biotechnology and Springer.
Ramya B.,Rajah Serfoji Government College Autonomous |
Malarvili T.,Rajah Serfoji Government College Autonomous |
Velavan S.,Marudupandiyar College
International Journal of Pharma and Bio Sciences | Year: 2015
The study was planned to analyse the vitamins and minerals present in Bryonopsis laciniosa fruit.Study revealed that the fruit contains Vitamins like C,D and E.Iron was found to be very much abundant and Calcium, Magnesium, Potassium, Chloride are in high amount. Sulphate and sodium are in a moderate level. Carbon, phosphorous, sodium, sulphur, zinc and manganese are substantially present while copper, Boron, selenium and molybdenum are present in trace amounts. These substances may be responsible for the health related properties which are based on their antioxidant, anticancer, antipyrectic, antiaphoretic, antidiabetic, anti-inflammation, antiheamatisum, antimicrobial and antiviral activity.
Nandhini R.,SASTRA University |
Jeyadoss T.,SASTRA University |
Velavan S.,Marudupandiyar College
International Journal of Pharma and Bio Sciences | Year: 2014
Liver plays a major role in detoxification. Any impairment to it or dysfunction may lead to many complication on one's health. Treatment of liver diseases is still a challenge to modern medicine. The commerecially available medicine has little to offer for the alleviation of hepatic ailments but sever side effects whereas the most important representatives are of phytoconstituents. The study was aimed to evaluate in vitro hepatoprotective activity of Aplotaxis auriculata rhizome (200 and 400μg/ml) through CCl4 induced toxicity in hepatocytes. The phytochemical screening revealed the presence of flavonoids, terpenoids, triterpenoids, polyphenol and tannins. All the variables tested as LPO, GSH, Protein, Bilirubin, ALP, SGOT and SGPT recorded a significant alteration observed in CCl4 exposed hepatocytes. However treatment with Aplotaxis auriculata extract restored the level and to near normal values was observed. The potential hepatoprotective activity of Aplotaxis auriculata is due to the presence of phytochemical constitution present in plant. Some of these phytochemicals such as flavonoids and polyphenolic compounds might have possessed hepatoprotective activity.
Senthamil Selvan P.,Marudupandiyar College |
Velavan S.,Marudupandiyar College
Rasayan Journal of Chemistry | Year: 2015
The aim of this study was to investigate the bioactive compounds from methanolic extract of Cissus vitiginea leavesby Gas chromatography and Mass spectroscopy (GC-MS). GC-MS analysis of methanolic extract was done bystandard protocol using the equipment Perkin-Elmer Gas Chromatography–Mass Spectrometry, while the massspectra of the compounds found in the extract was matched with the National Institute of Standards and Technology(NIST) library. The GC-MS analysis revealed the presence of various compounds like Tetradecanoic acid (19.658),3,7,11,15-Tetramethyl-2-hexadecen-1-ol (20.921), 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (21.144), Hexadecanoicacid, methyl ester (21.636), Oleic Acid (21.865), l-(+)-Ascorbic acid 2,6-dihexadecanoate(22.057), 9-Octadecenoicacid (22.712), Andrographolide (22.947), Heptadecanoic acid (23.106), Octadecanoic Acid, methyl ester (23.817),9,12-Octadecadienoic acid (24.552) and 22-Tricosenoic acid (26.836) in the methanolic extract of Cissus vitiginea.These findings support the traditional use of Cissus vitiginea in various disorders. ©2015 RASĀYAN. All rights reserved
Upgade A.,Center for Research and Development University |
Prabakaran P.,Marudupandiyar College
International Journal of Pharmacy and Technology | Year: 2015
Urinary tract infections are the widest spread illness in India. Poor understanding and limited diagnosis is the major cause of the infection. This can be divided in two sub categories, such as complicated and uncomplicated infections. Most of this are bacterial and fungal, their cause is due to easy access to soft tissues. If a person is immunocompromised and hospitalised the chances of this infection is more than 70%. Due to lack of knowledge and less focus there is limited therapeutics available. High dose of broad spectrum antibiotics also now a day’s getting resistance. Targeted therapy and natural drug selection is need of a hour. To achieve and develop new arena this pilot study was designed. Specific targets were selected from designated common occurring uropathogens namely Pseudomonas aeruginosa, Klebsiella Pneumoniae and Escherichia coli. Various active compounds were selected from plant leaves of C.papaya exibiting antimicrobial activity. Molecular docking studies were performed using Autodock 4.0 bioinformatics tool. All the compounds were screened for drug likeness activity as well as ADME analysis using bioinformatics software’s. All the ccompounds have been screened and docked for binding energy analysis, the potential docked structures are then recommended for further studies. © 2015, International Journal Of Pharmacy and Technology. All rights reserved.
Senthilkumar R.,Avvm Sri Pushpam College Autonomous |
Rajasekaran R.,Marudupandiyar College |
Selvaraj T.,Jimma University
Journal of Pure and Applied Microbiology | Year: 2010
Use of bioinoculants in agriculture horticulture and forestry greatly improve the survival and growth of plant species. The inoculated plants get increased tolerance to adverse soil ph, heavy metals, drought etc. The bioinoculants also help the plant for increased root development and enhances the biomass and yield. In the presence study the status of beneficial organisms like Arbuscular mycorrihizal fungus (AM), Ectomycorrihizal fungi (EMF) and Actinomycetes was investigated in the different age groups of Casuarina equisetifolia Plantations in Tamilnadu. Among different age group of the root samples screened, maximum percent root colonization of Arbuscular Mycorrhizal (AM) fungi was recorded in the root samples of one year old plantation (92%) this is followed by root sample of three year old plantation (67%). Minimum percent colonization was observed in four year old plantation sample (37%). The moderate percent colonization of AM fungi was seen in five and six year old plantation samples. No ectomycorrhizal fungal structures were recorded in the root samples of different age groups of plantations screened. Among different age group of the samples screened, maximum number of AM fungal spores (72/100 g soil) was recorded in the rhizosphere of one year plantation this is followed by two year old plantation (51/100g soil). The minimum number AM fungal spores was recorded in the rhizosphere of three year old plantation (33/100g soil). The C.equisetifolia and C.junghuhniana inoculated with various inoculants such as VAM, and Ectomychrrhizae P.albus and Laccaria fratera in various forms such as vermiculate form, basideospore form alginate bead form other then this also inoculate with frankia . Chemical fertilizers DAP in IFGTB nursery. Pisolithus albus basidiospore form shows the maximum growth in root (19.6) and shoot length (26.6) of Casuarina junghuhniana Pisolithus albus vermiculite form shows maximum length in both plants DAP shows second maximum Frankia inoculated plants shows maximum result in biomass of Casuarina equisetaifolia and its followed by Pisolithus albus vermiculite form for Casuarina Junghuhniana, the Pisolithas albus inoculated plant tissues maximum results and its followed by Pisolithas albus spores. Rhizobus of Causarina equisetifoloa and Casuarina junghuhniana inoculated with VAM spores. Maximum ECM colonization observed in treatment inoculated Pisolithus albus sopre. The study shows the bioinoculants treated plant shows greater improvement when compared with chemical fertilizer and we are in the need to create on awareness on use of biofertilizer among the former to improve the growth Casuarina and other tree crops.
Mahadevan K.,Tamil University |
Velavan S.,Marudupandiyar College
International Journal of Pharma and Bio Sciences | Year: 2013
Saliva is increasingly used and well validated in diagnosing, monitoring health and disease status, mainly due to its origin, composition, functions, and interactions with other organ systems. The effect of nutrition status on saliva covers a broad spectrum of factors. This includes the degree of malnutrition, the period when malnutrition takes place, the consistency of the diet, the effect on salivary gland growth and function, and the effects on the different salivary glands. Protein-energy malnutrition (PEM) is a serious nutritional problem in slum children's and elder. Serum albumin, globulin, transferring, total protein and amylase activity have been used as biochemical indicators of protein nutritional status and salivary gland functions, but taking blood from the slum subjects are difficult and invasive. We therefore assessed the possibility of using saliva as noninvasive materials to estimate concentrations of albumin, transferrin, total protein and amylase activity. Saliva is collected from 155 slum and healthy subjects of different ages and control (aged 8-15, 24-32, 58-77 yrs) and the concentrations of albumin, transferrin, total protein and amylase activity in saliva were evaluated. Altered levels of protein profile were observed in this study. This result suggested that nutritional deficiencies compromise salivary gland function, thus increasing susceptibility to dental caries and oral infections and reducing quality of life.