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Thiruvengadam M.,National Chung Hsing University | Thiruvengadam M.,Marudupandiyar College | Rekha K.T.,National Chung Hsing University | Yang C.-H.,National Chung Hsing University | And 2 more authors.
Plant Biotechnology Reports | Year: 2010

An efficient protocol for in vitro organogenesis was achieved from callus-derived immature and mature leaf explants of Momordica charantia, a very important vegetable and medicinal plant. Calluses were induced from immature leaf explants excised from in vitro (15-day-old seedlings) mature leaf explants of vivo plants (45 days old). The explants were grown on Murashige and Skoog (MS) medium with Gamborg (B5) vitamins containing 30 g l-1 sucrose, 2.2 g l-1 Gelrite, and 7.7 μM naphthalene acetic acid (NAA) with 2.2 μM thidiazuron (TDZ). Regeneration of adventitious shoots from callus (30-40 shoots per explant) was achieved on MS medium containing 5.5 μM TDZ, 2.2 μM NAA, and 3.3 μM silver nitrate (AgNO3). The shoots (1.0 cm length) were excised from callus and elongated in MS medium fortified with 3.5 μM gibberellic acid (GA3). The elongated shoots were rooted in MS medium supplemented with 4.0 μM indole 3-butyric acid (IBA). Rooted plants were acclimatized in the greenhouse and subsequently established in soil with a survival rate of 90%. This protocol yielded an average of 40 plants per leaf explant with a culture period of 98 days. © 2010 Korean Society for Plant Biotechnology and Springer.


Upgade A.,Center for Research and Development University | Prabakaran P.,Marudupandiyar College
International Journal of Pharmacy and Technology | Year: 2015

Urinary tract infections are the widest spread illness in India. Poor understanding and limited diagnosis is the major cause of the infection. This can be divided in two sub categories, such as complicated and uncomplicated infections. Most of this are bacterial and fungal, their cause is due to easy access to soft tissues. If a person is immunocompromised and hospitalised the chances of this infection is more than 70%. Due to lack of knowledge and less focus there is limited therapeutics available. High dose of broad spectrum antibiotics also now a day’s getting resistance. Targeted therapy and natural drug selection is need of a hour. To achieve and develop new arena this pilot study was designed. Specific targets were selected from designated common occurring uropathogens namely Pseudomonas aeruginosa, Klebsiella Pneumoniae and Escherichia coli. Various active compounds were selected from plant leaves of C.papaya exibiting antimicrobial activity. Molecular docking studies were performed using Autodock 4.0 bioinformatics tool. All the compounds were screened for drug likeness activity as well as ADME analysis using bioinformatics software’s. All the ccompounds have been screened and docked for binding energy analysis, the potential docked structures are then recommended for further studies. © 2015, International Journal Of Pharmacy and Technology. All rights reserved.


Nandhini R.,SASTRA University | Jeyadoss T.,SASTRA University | Velavan S.,Marudupandiyar College
International Journal of Pharma and Bio Sciences | Year: 2014

Liver plays a major role in detoxification. Any impairment to it or dysfunction may lead to many complication on one's health. Treatment of liver diseases is still a challenge to modern medicine. The commerecially available medicine has little to offer for the alleviation of hepatic ailments but sever side effects whereas the most important representatives are of phytoconstituents. The study was aimed to evaluate in vitro hepatoprotective activity of Aplotaxis auriculata rhizome (200 and 400μg/ml) through CCl4 induced toxicity in hepatocytes. The phytochemical screening revealed the presence of flavonoids, terpenoids, triterpenoids, polyphenol and tannins. All the variables tested as LPO, GSH, Protein, Bilirubin, ALP, SGOT and SGPT recorded a significant alteration observed in CCl4 exposed hepatocytes. However treatment with Aplotaxis auriculata extract restored the level and to near normal values was observed. The potential hepatoprotective activity of Aplotaxis auriculata is due to the presence of phytochemical constitution present in plant. Some of these phytochemicals such as flavonoids and polyphenolic compounds might have possessed hepatoprotective activity.


Senthilkumar R.,AVVM Sri Pushpam College Autonomous | Rajasekaran R.,Marudupandiyar College | Selvaraj T.,Jimma University
Journal of Pure and Applied Microbiology | Year: 2010

Use of bioinoculants in agriculture horticulture and forestry greatly improve the survival and growth of plant species. The inoculated plants get increased tolerance to adverse soil ph, heavy metals, drought etc. The bioinoculants also help the plant for increased root development and enhances the biomass and yield. In the presence study the status of beneficial organisms like Arbuscular mycorrihizal fungus (AM), Ectomycorrihizal fungi (EMF) and Actinomycetes was investigated in the different age groups of Casuarina equisetifolia Plantations in Tamilnadu. Among different age group of the root samples screened, maximum percent root colonization of Arbuscular Mycorrhizal (AM) fungi was recorded in the root samples of one year old plantation (92%) this is followed by root sample of three year old plantation (67%). Minimum percent colonization was observed in four year old plantation sample (37%). The moderate percent colonization of AM fungi was seen in five and six year old plantation samples. No ectomycorrhizal fungal structures were recorded in the root samples of different age groups of plantations screened. Among different age group of the samples screened, maximum number of AM fungal spores (72/100 g soil) was recorded in the rhizosphere of one year plantation this is followed by two year old plantation (51/100g soil). The minimum number AM fungal spores was recorded in the rhizosphere of three year old plantation (33/100g soil). The C.equisetifolia and C.junghuhniana inoculated with various inoculants such as VAM, and Ectomychrrhizae P.albus and Laccaria fratera in various forms such as vermiculate form, basideospore form alginate bead form other then this also inoculate with frankia . Chemical fertilizers DAP in IFGTB nursery. Pisolithus albus basidiospore form shows the maximum growth in root (19.6) and shoot length (26.6) of Casuarina junghuhniana Pisolithus albus vermiculite form shows maximum length in both plants DAP shows second maximum Frankia inoculated plants shows maximum result in biomass of Casuarina equisetaifolia and its followed by Pisolithus albus vermiculite form for Casuarina Junghuhniana, the Pisolithas albus inoculated plant tissues maximum results and its followed by Pisolithas albus spores. Rhizobus of Causarina equisetifoloa and Casuarina junghuhniana inoculated with VAM spores. Maximum ECM colonization observed in treatment inoculated Pisolithus albus sopre. The study shows the bioinoculants treated plant shows greater improvement when compared with chemical fertilizer and we are in the need to create on awareness on use of biofertilizer among the former to improve the growth Casuarina and other tree crops.


Ramya B.,Rajah Serfoji Government College Autonomous | Malarvili T.,Rajah Serfoji Government College Autonomous | Velavan S.,Marudupandiyar College
International Journal of Pharma and Bio Sciences | Year: 2015

The study was planned to analyse the vitamins and minerals present in Bryonopsis laciniosa fruit.Study revealed that the fruit contains Vitamins like C,D and E.Iron was found to be very much abundant and Calcium, Magnesium, Potassium, Chloride are in high amount. Sulphate and sodium are in a moderate level. Carbon, phosphorous, sodium, sulphur, zinc and manganese are substantially present while copper, Boron, selenium and molybdenum are present in trace amounts. These substances may be responsible for the health related properties which are based on their antioxidant, anticancer, antipyrectic, antiaphoretic, antidiabetic, anti-inflammation, antiheamatisum, antimicrobial and antiviral activity.

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