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Ōsaka, Japan

Mawatari S.,Institute of Rheological Function of Food | Katafuchi T.,Kyushu University | Miake K.,Marudai Food Co. | Fujino T.,Institute of Rheological Function of Food
Lipids in Health and Disease

Background: Many disorders with plasmalogen deficiency have been reported. Replenishment or replacement of tissue plasmalogens of these disorders would be beneficial to the patients with these disorders, but effects of dietary plasmalogen on mammals have not been reported. Methods. Plasmalogens were purified from chicken skin. The purified plasmalogens consisted of 96.4% ethanolamine plasmalogen (PlsEtn), 2.4% choline plasmalogen (PlsCho) and 0.5% sphingomyelin (SM). A diet containing 0.1% the purified plasmalogens (PlsEtn diet) was given to rats. Relative composition of phospholipids was measured by a high performance liquid chromatography (HPLC) method that can separate intact plasmalogens and all other phospholipid classes by a single chromatographic run. Results: The PlsEtn diet given to Zucker diabetic fatty (ZDF) rats for 4 weeks caused decreases of plasma cholesterol and plasma phospholipid as compared to control diet. The other routine laboratory tests of plasma including triacylglycerol, glucose, liver and renal functions, albumin, and body weight were not different. Relative compositions of erythrocyte PlsEtn and phosphatidylethanolamine (PE) increased, and that of phosphatidylcholine (PC) decreased in PlsEtn diet group. The PlsEtn diet given to normal rats for 9 weeks again caused decrease of plasma cholesterol and phospholipid, and it induced increase of relative composition of PlsEtn of the erythrocyte membrane. The other routine laboratory tests of plasma and body weight were not different. Conclusions: Dietary PlsEtn increases relative composition of PlsEtn of erythrocyte membranes in normal and ZDF rats, and it causes decreases of plasma cholesterol and plasma phospholipids. Dietary PlsEtn for 9 weeks seemingly causes no adverse effect to health of normal rats. © 2012 Mawatari et al.; licensee BioMed Central Ltd. Source

Fujino Brain Research Co., Umeda Jimusho Ltd. and Marudai Food Co. | Date: 2011-09-22

An objective of the present invention is to provide a novel method with an effect of alleviating central nervous system inflammation. The present invention provides a drug against central nervous system inflammation containing a plasmalogen. More preferably, the present invention provides a drug against central nervous system inflammation containing a plasmalogen extracted from a biological tissue (preferably an avian tissue) that mainly contains an ethanolamine plasmalogen and a choline plasmalogen.

Kawamura J.,Marudai Food Co. | Kawamura J.,Iwate University | Kotoura S.,Marudai Food Co. | Okuyama T.,Marudai Food Co. | And 6 more authors.
Nippon Shokuhin Kagaku Kogaku Kaishi

We investigated the effects of defatted-dried chicken skin powder (DCS) containing sphingomyelin (SM) on human facial skin conditions. A placebo-controlled, double-blind 12-week study of DCS (1 g/day ; SM2mg/day) was conducted on 36 female volunteers who tend to have dry skin. In the DCS group, the moisture of the stratum corneum of cheek tended to increase after 12 weeks. In the subjects whose moisture of cheek was less than 35 arbitrary unit, the DCS group was significantly higher than the placebo group. After 12 weeks, skin elasticity showed significantly increase in the DCS group compared with 0w. These results indicate that the ingestion of DCS improves dry facial skin. Source

Sakai Y.,Oriental Yeast Co. | Kotoura S.,Marudai Food Co. | Yano T.,Oriental Yeast Co. | Yano T.,Mie University | And 5 more authors.
Bioscience, Biotechnology and Biochemistry

A standard plasmid was constructed as a novel reference molecule for use in real-time quantitative PCR assays to verify the identity of beef, pork, chicken, mutton, and horseflesh. The plasmid contained a target domain of the cytochrome b (cyt b) gene and an artificial DNA sequence. Primers CO-F and CO-R, and probe CO-P were specifically designed to detect the artificial sequence. The calculated R 2 values of the standard curves (10 3-10 7 copies per reaction) for the five species ranged between 0.998 and 0.999 in the quantification analysis. The constructed plasmid provides a universal method for measuring the copy number of cyt b DNA in minced meat. This method would be a useful procedure for verifying food labels. Source

Katafuchi T.,Kyushu University | Ifuku M.,Kyushu University | Mawatari S.,Institute of Rheological Function of Food | Noda M.,Kyushu University | And 3 more authors.
Annals of the New York Academy of Sciences

Neuroinflammation essentially involves an activation of glial cells as the cause/effect of neurodegenerative diseases such as Alzheimer's disease (AD). Plasmalogens (Pls) are glycerophospholipids constituting cellular membranes and play significant roles in membrane fluidity and cellular processes like vesicular fusion and signal transduction. Intraperitoneal (i.p.) injection of lipopolysaccharide (LPS, 250 μg/kg) for 7 days resulted in the morphological changes and increase in number of Iba-1+ microglia showing neuroinflammation in the adult mouse hippocampus. The LPS-induced activation of glial cells was significantly attenuated by i.p. pretreatment with Pls dissolved in corn oil. In addition, systemic injection of LPS induced Aβ1-16 + neurons in the hippocampus were also abolished by application of Pls. Finally, contents of Pls in the hippocampus decreased after LPS injection, and the reduction was suppressed by administration of Pls. These findings suggest an antiamyloidogenic effect of Pls, implicating a possible therapeutic application of Pls against AD. © 2012 New York Academy of Sciences. Source

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