Marker Assisted Selection Laboratory

Bangalore, India

Marker Assisted Selection Laboratory

Bangalore, India
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Raghavan R.,Presidency College at Chennai | Elumalai S.,Presidency College at Chennai | Nirmal Babu K.,Indian Institute of Spices Research | Hittalmani S.,Marker Assisted Selection Laboratory
Biosciences Biotechnology Research Asia | Year: 2010

A set of 20 Piper nigrum (Black Pepper) accessions were screened to identify the extent of genetic diversity present at the Molecular level using RAPD and SSR markers.. Dendogram constructed based on molecular polymorphism unveiled considerable amount of diversity among the varieties. Among this the SSR markers were found to be useful in discrimination and identification of the genotypes as it gave more genotypic specific bands.


Nagaraja Reddy R.,Directorate of Sorghum Research | Nagaraja Reddy R.,Directorate of Medicinal and Aromatic Plants Research | Madhusudhana R.,Directorate of Sorghum Research | Madhusudhana R.,Marker Assisted Selection Laboratory | And 10 more authors.
Indian Journal of Agricultural Sciences | Year: 2011

Abstract: EST-SSR markers are the popular markers due to their rapid in silico development and high cross-species and genera transferability. We assessed sorghum EST-SSR markers for their transferability into its wild species and other members of Gramineae family. The inter specific transferability of sorghum EST-SSRs ranged between 92.8% (S. usumbarense, S. propinquum, para sorghum) and 100% (S. halepense) while inter-generic transferability ranged from 78.5% (oats and pearl millet) to 100% (sugarcane). Six out of 14 markers were polymorphic among S. bicolor genotypes while 12 were polymorphic between wild species of sorghum. Dendrogram revealed seven groups differentiating Gramineae members. In silico comparison of SSR-ESTs of sorghum, maize and rice showed either intact or disrupted SSR motif in homologus position. This small set of sorghum EST-SSR markers with high transferability across species and genera will be highly useful for assessing the functional diversity, comparative mapping and other applications in crops with very limited markers.

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