Noyer C.,Marine Resource Center |
Noyer C.,CSIC - Center for Advanced Studies of Blanes |
Hamilton A.,Marine Resource Center |
Hamilton A.,European Center for Marine Biotechnology |
And 2 more authors.
Symbiosis | Year: 2010
Marine sponges can host in their tissues abundant and diverse bacterial communities. Lack of truly quantitative data on bacterial abundance and dynamics limits our understanding of the organization and functioning of these endobiotic communities. In this technical note, we describe a quantitative polymerase chain reaction approach to quantify the relative abundance of multiple clades of three major sponge-associated bacterial phyla: Chloroflexi, Acidobacteria, and Actinobacteria. To test our approach we used the Mediterranean sponges Spongia lamella and Aplysina aerophoba. We designed five out of the six primer sets used in our study. We tested the new primer sets for specificity and optimized their conditions. Our preliminary data showed that Spongia lamella had larger bacterial abundance than Aplysina aerophoba, except for one clade of Chloroflexi. The two Chloroflexi clades investigated in our study amplified a fraction of the Chloroflexi present in Spongia lamella and most of what is present in Aplysina aerophoba, suggesting a more diverse Chloroflexi population in Spongia lamella than in Aplysina aerophoba. This quantitative technique has a great potential to provide a rapid and robust assessment of sponge microbial target and could contribute to deciphering the complexity of these largely unknown host-symbiont interactions. © 2010 Springer Science+Business Media B.V.
Mohammady N.G.-E.,Alexandria University |
Mohammady N.G.-E.,Marine Resource Center |
Rieken C.W.,Josephine Bay Paul Center |
Lindell S.R.,Marine Resource Center |
And 5 more authors.
Research Journal of Phytochemistry | Year: 2012
A variety of batch cultured marine microalgae was selected as lipid producer candidates towards the biofuel invention. The strains were characterized and compared for their growth and potential lipid production. The results showed that, under basal cultivation conditions, Tetraselmis chuii is the highest grown and lowest lipid producer among the investigated species. Furthermore, a linear correlation between the alga's cell size and the lipid content was demonstrated. However, after being transferred into nitrogen deficient nutrient medium, T. chuii cells were equally sized and attained the maximum size after 15 days of cultivation. In addition, the lipid amount produced by the alga showed an increase by about 35% compared to the basal growth medium. © 2012 Academic Journels Inc.