Marine Products Kimuraya Co.

Sakaiminato, Japan

Marine Products Kimuraya Co.

Sakaiminato, Japan
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Sekiguchi T.,Kyushu University | Kamada Y.,Japan National Institute for Basic Biology | Furuno N.,Hiroshima University | Funakoshi M.,Okayama University | And 2 more authors.
Genes to Cells | Year: 2014

The yeast Ras-like GTPases Gtr1p and Gtr2p form a heterodimer, are implicated in the regulation of TOR complex 1 (TORC1) and play pivotal roles in cell growth. Gtr1p and Gtr2p bind Ego1p and Ego3p, which are tethered to the endosomal and vacuolar membranes where TORC1 functions are regulated through a relay of amino acid signaling interactions. The mechanisms by which Gtr1p and Gtr2p activate TORC1 remain obscure. We probed the interactions of the Gtr1p-Gtr2p complex with the Ego1p-Ego3p complex and TORC1 subunits. Mutations in the region (179-220 a.a.) following the nucleotide-binding region of Gtr1p and Gtr2p abrogated their mutual interaction and resulted in a loss in function, suggesting that complex formation between Gtr1p and Gtr2p was indispensable for TORC1 function. A modified yeast two-hybrid assay showed that Gtr1p-Gtr2p complex formation is important for its interaction with the Ego1p-Ego3p complex. GTP-bound Gtr1p interacted with the region containing the HEAT repeats of Kog1p and the C-terminal region of Tco89p. The GTP-bound Gtr2p suppressed a Kog1p mutation. Our findings indicate that the interactions of the Gtr1p-Gtr2p complex with the Ego1p-Ego3p complex and TORC1 components Kog1p and Tco89p play a role in TORC1 function. © 2014 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd.


Morimoto M.,Tottori University | Takatori M.,Tottori University | Hayashi T.,Tottori University | Mori D.,Tottori University | And 8 more authors.
Carbohydrate Research | Year: 2014

Fucoidan and chondroitin sulfate, which are well known sulfated polysaccharides, were depolymerized under hydrothermal conditions (120-180 C, 5-60 min) as a method for the preparation of sulfated polysaccharides with controlled molecular weights. Fucoidan was easily depolymerized, and the change of the molecular weight values depended on the reaction temperature and time. The degree of sulfation and IR spectra of the depolymerized fucoidan did not change compared with those of untreated fucoidan at reaction temperatures below 140 C. However, fucoidan was partially degraded during depolymerization above 160 C. Nearly the same depolymerization was observed for chondroitin sulfate. These results indicate that hydrothermal treatment is applicable for the depolymerization of sulfated polysaccharides, and that low molecular weight products without desulfation and deformation of the initial glycan structures can be obtained under mild hydrothermal conditions.©2013 Elsevier Ltd. All rights reserved.


PubMed | Marine Products Kimuraya Co., Koyo Chemical Co., Tottori Institute of Industrial Technology, Tottori University and Scientific Crime Laboratory
Type: | Journal: Carbohydrate research | Year: 2014

Fucoidan and chondroitin sulfate, which are well known sulfated polysaccharides, were depolymerized under hydrothermal conditions (120-180C, 5-60min) as a method for the preparation of sulfated polysaccharides with controlled molecular weights. Fucoidan was easily depolymerized, and the change of the molecular weight values depended on the reaction temperature and time. The degree of sulfation and IR spectra of the depolymerized fucoidan did not change compared with those of untreated fucoidan at reaction temperatures below 140C. However, fucoidan was partially degraded during depolymerization above 160C. Nearly the same depolymerization was observed for chondroitin sulfate. These results indicate that hydrothermal treatment is applicable for the depolymerization of sulfated polysaccharides, and that low molecular weight products without desulfation and deformation of the initial glycan structures can be obtained under mild hydrothermal conditions.


Ohshiro T.,Tottori University | Ohmoto Y.,Tottori University | Ono Y.,Tottori University | Ohkita R.,Tottori University | And 3 more authors.
Bioscience, Biotechnology and Biochemistry | Year: 2010

A bacterium utilizing fucoidan from the brown alga Cladosiphon okamuranus as sole carbon source was isolated and identified as Flavobacterium sp. F-31. The strain produced intracellular enzymes involved in fucoidan degradation and desulfation, but desulfation activity was not detected until the molecular weight of fucoidan fell to less than several tens of thousands due to enzymatic degradation. Only fucoidan proved to be an inducible substance for the production of the degrading enzymes.


Ohshiro T.,Tottori University | Harada N.,Tottori University | Kobayashi Y.,Tottori University | Miki Y.,Marine Products Kimuraya Co. | Kawamoto H.,Marine Products Kimuraya Co.
Bioscience, Biotechnology and Biochemistry | Year: 2012

A bacterial strain that assimilates fucoidan from Cladosiphon okamuranus as sole carbon source was isolated as Luteolibacter algae H-18. It was found that it degraded fucoidan by intracellular enzymes, and that the degradation reactions were catalyzed by multiple enzymes. One enzyme, designated fraction B, was established to exhibit the deacetylation reaction of fucoidan. Other enzyme(s), designated fraction A, catalyzed the reaction(s) lowering the molecular weight of fucoidan.


Abe S.,Marine Products Kimuraya Co. | Hiramatsu K.,Tottori University | Ichikawa O.,Tottori University | Kawamoto H.,Marine Products Kimuraya Co. | And 4 more authors.
Journal of Food Science | Year: 2013

Fucoidan is a sulfated polysaccharide in the brown sea algae. This study was conducted in 20 subjects taking excessive fucoidan up to 4.05 g daily for 2 wk. They recorded questionnaire sheets about their health. Blood and urine were collected before and after 2 wk of ingestion. We found that no disorder was apparent in the test period. Although total cholesterol (P value 0.017) and low-density lipoprotein cholesterol (P value 0.017) showed statistically significant reduction and Cl (P value 0.002) showed significant increase, nothing deviated from the range of normal values. In conclusion, this study showed no abnormalities in the abdominal, fecal states, blood and urine at all. © 2013 Institute of Food Technologists®.


Ikeguchi M.,Tottori University | Yamamoto M.,Tottori University | Arai Y.,Tottori University | Maeta Y.,Tottori University | And 4 more authors.
Oncology Letters | Year: 2011

Combination chemotherapy with oxaliplatin plus 5-fluorouracil/leucovorin (FOLFOX) or irinotecan plus 5-fluo- rouracil/leucovorin (FOLFIRI) has become a standard regimen for advanced or recurrent colorectal cancer. Numerous studies have reported that long-term use of FOLFOX or FOLFIRI leads to better survival for these patients. Thus, control of the toxicity of these drugs may be crucial to prolonging survival. Fucoidan is one of the major sulfated polysaccharides of brown seaweeds and exhibits a wide range of biological activities. In the present study, we analyzed the effect of fucoidan on suppressing the toxicity of anti-cancer drugs. A total of 20 patients with unresectable advanced or recurrent colorectal cancer scheduled to undergo treatment with FOLFOX or FOLFIRI were randomly allocated into a fucoidan treatment group (n=10) and a control group without fucoidan treatment (n=10). Results showed that fucoidan regulated the occurrence of fatigue during chemotherapy. Chemotherapy with fucoidan was continued for a longer period than chemotherapy without fucoidan. Additionally, the survival of patients with fucoidan treatment was longer than that of patients without fucoidan, although the difference was not significant. Thus, fucoidan may enable the continuous administration of chemotherapeutic drugs for patients with unresectable advanced or recurrent colorectal cancer, and as a result, the prognosis of such patients is prolonged.


Patent
Tottori University and Marine Products Kimuraya Co. | Date: 2014-07-10

Disclosed is a therapy which is for preventing or treating cartilage damage and diseases associated with cartilage damage, such as arthritides and osteoarthritis, and utilizes a more effective and more safe medicinal agent. Specifically disclosed are a cartilage production promoter, a glucosaminoglycan and/or proteoglycan production promoter, and a prophylactic or therapeutic agent for diseases associated with cartilage damage, each of which comprises fucoidan as an active ingredient.


The present invention provides a food/drink or pharmaceutical composition for oral administration for improving an acidic urine, comprising fucoidan or a fucoidan-containing material as an active ingredient, characterized in that a urinal pH is persistently increased; use of fucoidan or a fucoidan-containing material for production of the food/drink or the pharmaceutical composition for oral administration; and a method for improving an acidic urine by persistently increasing a urinal pH of a patient, which comprises orally administering fucoidan or a fucoidan-containing material to a patient.


Patent
Tottori University and Marine Products Kimuraya Co. | Date: 2012-08-08

The present invention provides a side effect inhibitor of drug therapy for colorectal cancer using 5-fluorouracil, comprising fucoidan or a fucoidan-containing material; a method for inhibiting side effects of drug therapy for colorectal cancer using 5-fluorouracil, which comprises administering fucoidan or a fucoidan-containing material to a colorectal cancer patient; and use of fucoidan or a fucoidan-containing material for the production of a side effect inhibitor of drug therapy for colorectal cancer using 5-fluorouracil.

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