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News Article | December 13, 2016

ABU DHABI, UAE, December 13, 2016 /PRNewswire/ -- Miral partners with SeaWorld to create a next-generation marine life theme parkand the UAE's first Marine Life Research, Rescue, Rehabilitation and Return Center by 2022 Miral and SeaWorld Entertainment, Inc. (NYSE:SEAS)...

News Article | December 13, 2016

ABU DHABI, UAE, December 13, 2016 /PRNewswire/ -- Miral partners with SeaWorld to create a next-generation marine life theme parkand the UAE's first Marine Life Research, Rescue, Rehabilitation and Return Center by 2022 Miral and SeaWorld Entertainment, Inc. (NYSE:SEAS)...

Lee J.S.,Chosun University | Jung W.-K.,Marine Life Research | Jeong M.H.,Chonnam National University | Yoon T.R.,Chonnam National University | Kim H.K.,Chonnam National University
International Journal of Toxicology | Year: 2012

Sanguinarine is an alkaloid obtained from the bloodroot plant Sanguinaria canadensis and has beneficial effects on oxidative stress and inflammatory disorders. Previous reports have demonstrated that sanguinarine also exhibit anticancer properties. In the current study, we investigated the effects of sanguinarine on HT-29 human colon cancer cells. It was observed that sanguinarine treatment induces a dose-dependent increase in apoptosis of human colon cancer cells. We also investigated the effects of sanguinarine on the expression of apoptosis-associated proteins, and the results revealed that there was an increase in Bax and a decrease in B-cell lymphoma 2 (Bcl-2) protein levels. Moreover, sanguinarine treatment significantly increases the activation of caspases 3 and 9 that are the key executioners in apoptosis. Our results suggest that sanguinarine induces apoptosis of HT-29 human colon cancer cells and may have a potential therapeutic use in the treatment of human colon cancer. © 2011 American College of Toxicology.

Kang K.-H.,Pukyong National University | Qian Z.-J.,Marine Life Research | Ryu B.,Pukyong National University | Karadeniz F.,Pukyong National University | And 2 more authors.
Phytotherapy Research | Year: 2012

Marine microalgae have been reported as valuable new sources of pharmacologically active compounds and there are now numerous commercial applications of microalgae. Hence, in this study we evaluated the protective effects of peptides purified from marine microalgae, Navicula incerta, against alcohol-induced damage in HepG2/CYP2E1 cells. To obtain bioactive peptides from microalgae, N. incerta was hydrolysed using various enzymes (alcalase, α-chymotrypsin, neutrase, papain, pepsin, pronase-E and trypsin), and the hydrolysates were evaluated for cytoprotective activity. Among them, papain-derived hydrolysate exhibited higher antioxidant activities than those of other enzymes. Therefore, papain hydrolysate was purified in order to obtain potent antihepatotoxic and antioxidative peptides. The amino acid sequences of the purified peptides were analysed as; NIPP-1 (Pro-Gly-Trp-Asn-Gln-Trp-Phe-Leu) with molecular mass 1 171 Da, and NIPP-2 (Val-Glu-Val-Leu-Pro-Pro-Ala-Glu-Leu) with molecular mass 1108 Da. Furthermore, this study demonstrated that NIPP-1 and NIPP-2 peptides inhibited ethanol-induced cytotoxicity in HepG2/CYP2E1 cells. Copyright © 2012 John Wiley & Sons, Ltd.

Himaya S.W.A.,Pukyong National University | Ryu B.,Pukyong National University | Qian Z.-J.,Pukyong National University | Qian Z.-J.,Marine Life Research | Kim S.-K.,Pukyong National University
Toxicology in Vitro | Year: 2012

Inflammation has recently been implicated as a critical mechanism responsible for neurodegenerative diseases. In this study, paeonol (1-(2-hydroxy-4-methoxyphenyl)ethanone) isolated from the sea horse Hippocampus kuda Bleeler was studied as an agent to suppress LPS induced activation of BV-2 microglial and RAW264.7 macrophage cells. The results obtained showed that paeonol significantly suppressed LPS induced release of pro-inflammatory products such as nitric oxide (NO), prostaglandin E2 (PGE2), and cytokines; tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6). Furthermore, the compound down regulated the protein and gene expression levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), TNF-α, IL-1β and IL-6 in both cell lines. Molecular signaling pathway studies showed that paeonol inhibited the translocation of nuclear factor-κB (NF-κB) p65 and p50 subunits to the nucleus by blocking IKKα/β (IκB kinase α/β) mediated degradation of IκBα. Moreover, it suppressed the phosphorylation of mitogen activated protein kinase (MAPK) pathway molecules; c-Jun N-terminal kinases (JNK) and p38 in both cell lines. Collectively these results indicate that paeonol blocked the LPS stimulated inflammatory responses in BV-2 and RAW264.7 cells via modulating MAPK and NF-κB signaling pathways. Therefore, paeonol could be a promising candidate to be used in neuro-inflammatory therapy. © 2012 Elsevier Ltd.

Kang K.-H.,Pukyong National University | Qian Z.-J.,Marine Life Research | Ryu B.,Pukyong National University | Kim D.,Korea Basic Science Institute | Kim S.-K.,Pukyong National University
Food Chemistry | Year: 2012

Ethanol is independently known to cause tissue damage through various mechanisms. This study was designed to evaluate the protective effect of marine microalgae, Navicula incerta protein enzymatic hydrolysates (NEHs) against ethanol-induced hepatotoxicity in HepG2 cells transfected with human CYP2E1. Induction of CYP2E1 by ethanol is one of the central pathways by which ethanol generates a state of oxidative stress in hepatocytes. When the alcohol-induced cells were treated with NEHs at various concentrations, there was a dose-dependent decrease of gamma-glutamyl transpeptidase (GGT) activity in the culture media and loss of cell viability. Among the NEHs constituents the hydrolysates which were obtained by papain (P-NEH), pronase-E (PR-NEH) and α-chymotrypsin (A-NEH) activity attenuated the ethanol cytotoxicity effectively, respectively. The activity appeared to be a GGT inhibitor. Therefore, the cytoprotective effects at alcohol-induced HepG2/CYP2E1 cells could be attributed to the inhibition of GGT activity by NEHs. This study suggests that NEHs have enough potential to be considered as highly active compounds against ethanol toxicity which leads NEHs to be significant nutraceuticals. © 2011 Elsevier Ltd. All rights reserved.

Ryu B.,Pukyong National University | Kang K.-H.,Pukyong National University | Ngo D.-H.,Pukyong National University | Qian Z.-J.,Marine Life Research | Kim S.-K.,Pukyong National University
Bioresource Technology | Year: 2012

In this study, sequential strategy based design was applied to optimize the microalgae, Pavlova lutheri mass culture conditions and fermentation conditions of the cultured algae by proteolytic yeast Candidia rugopelliculosa to obtain small peptide chains. This optimization of culture and fermentation conditions by response surface methodology (RSM) finally leads to effective purification of a bioactive peptide MPGPLSPL (793.01. Da) with hydroxyl radical scavenging activity. Collectively, these results indicated that microalgae P. lutheri can enhance the hydroxyl radical inhibiting effect through protein hydrolysis process under RSM optimal condition. © 2011 Elsevier Ltd.

Qian Z.-J.,Marine Life Research | Kang K.-H.,Pukyong National University | Kim S.-K.,Pukyong National University
Biotechnology and Bioprocess Engineering | Year: 2012

Seahorse (Hippocampus Kuda Bleeler) has been used as traditional medicine for thousands of years, in Eastern Asia. In this study of the methanol extract of fresh Hippocampus Kuda, the new compounds 2-ethyldecyl 2- ethylundecyl phthalate (1), 2, 12-diethyl-11-methylhexadecyl 2-ethyl-11- methylhexadecylphthalate (2), along with a known Bis(2-ethylheptyl) phthalate (3) were isolated. They were tested for their antioxidant activities, including lipid peroxidation inhibitory activity, DPPH radical scavenging, hydroxyl radical scavenging, superoxide anion radical scavenging, alkyl radical scavenging, and cellular radicals; these can be detected using a fluorescence probe, 2',7'- dichlorofluorescin diacetate (DCFH-DA), which could be converted to highly fluorescent dichlorofluorescein (DCF) with the presence of intracellular ROS on mouse macrophages, RAW264.7 cell. Compound (2) exhibited the highest antioxidant activity and inhibitory intracellular ROS than another compounds (1, 3). Furthermore, MTT assay showed no cytotoxicity on mouse macrophages cell (RAW264.7) and human fetal lung fibroblast cell line (MRC-5). This antioxidant property depends on concentration and increasing with increased amount of the compound. © The Korean Society for Biotechnology and Bioengineering and Springer 2012.

Kang K.-H.,Pukyong National University | Ryu B.,Pukyong National University | Kim S.-K.,Pukyong National University | Qian Z.-J.,Marine Life Research
Food Science and Biotechnology | Year: 2011

Microalgae are major primary producers of organic matters in aquatic environments through their photosynthetic activities. Benthic diatom Navicula incerta is the major component of phytoplankton and also relatively easy to cultivate, used as live food source in aquaculture. The growth characteristics of N. incerta were estimated under combinations of temperature, salinity, and light; and also its composition and antioxidant activities were determined. The maximum cell density of 87×105cells/ mL, was reached at 20°C, 250 μmol/m2·sec, 33‰ salinity, pH 8.3, 12:12 light:dark, and F/2 medium on 2 weeks of the culture period. The antioxidant enzymatic hydrolysates efficiently quenched different free radicals: 1,1-diphenyl-2- pycryl-hydrazyl (DPPH) (pepsin IC50=196.0 μg/mL), hydroxyl (α-chymotrypsin IC50=102.0 μg/mL), and superoxide (neutrase IC50=169.0 μg/mL). These results suggest that the enzymatic hydrolysate from N. incerta acts as a candidate against antioxidant and could be used as a potential functional food ingredient. © KoSFoST and Springer 2011.

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