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News Article | December 13, 2016
Site: www.prnewswire.com

ABU DHABI, UAE, December 13, 2016 /PRNewswire/ -- Miral partners with SeaWorld to create a next-generation marine life theme parkand the UAE's first Marine Life Research, Rescue, Rehabilitation and Return Center by 2022 Miral and SeaWorld Entertainment, Inc. (NYSE:SEAS)...


News Article | December 13, 2016
Site: www.prnewswire.co.uk

ABU DHABI, UAE, December 13, 2016 /PRNewswire/ -- Miral partners with SeaWorld to create a next-generation marine life theme parkand the UAE's first Marine Life Research, Rescue, Rehabilitation and Return Center by 2022 Miral and SeaWorld Entertainment, Inc. (NYSE:SEAS)...


Moriyama K.,Matsumoto Dental University | Watanabe S.,Marine Life Research | Iida M.,Marine Life Research | Sahara N.,Matsumoto Dental University
Cell and Tissue Research | Year: 2010

Sicyopterus japonicus (Teleostei, Gobiidae) possesses a unique upper jaw dentition different from that known for any other teleosts. In the adults, many (up to 30) replacement teeth, from initiation to attachment, are arranged orderly in a semicircular-like strand within a capsule of connective tissue on the labial side of each premaxillary bone. We have applied histological, ultrastructural, and three-dimensional imaging from serial sections to obtain insights into the distribution and morphological features of the dental lamina in the upper jaw dentition of adult S. japonicus. The adult fish has numerous permanent dental laminae, each of which is an infolding of the oral epithelium at the labial side of the functional tooth and forms a thin plate-like structure with a wavy contour. All replacement teeth of a semicircular-like strand are connected to the platelike dental lamina by the outer dental epithelium and form a tooth family; neighboring tooth families are completely separated from each other. The new tooth germ directly buds off from the ventro-labial margin of the dental lamina, whereas no distinct free end of the dental lamina is present, even adjacent to this region. Cell proliferation concentrated at the ventro-labial margin of the dental lamina suggests that this region is the site for repeated tooth initiation. During tooth development, the replacement tooth migrates along a semicircular-like strand and eventually erupts through the dental lamina into the oral epithelium at the labial side of the functional tooth. This unique thin plate-like permanent dental lamina and the semicircular-like strand of replacement teeth in the upper jaw dentition of adult S. japonicus probably evolved as a dental adaptation related to the rapid replacement of teeth dictated by the specialized feeding habit of this algae-scraping fish. © Springer-Verlag 2010.


Couto T.,Marine Life Research | Duarte B.,University of Lisbon | Barroso D.,Marine Life Research | Cacador I.,University of Lisbon | Marques J.C.,Marine Life Research
Functional Plant Biology | Year: 2013

Heavy metal concentrations present in the above-and beowground tissues of Scirpus maritimus L., Spartina maritima (Curtis) Fernald and Zostera noltii Hornem were analysed seasonally in the Mondego Estuary, Portugal. The sediments of the estuary were confirmed to contain only low concentrations of heavy metals. The belowground tissues of all three species showed higher heavy metal concentrations than the aboveground tissues. Although the sediments only contained low levels of contamination, because the area occupied by S. maritimus and Z. noltii was large, significant quantities of heavy metals were accumulated and exported to the surrounding water bodies. In contrast with observations of highly contaminated estuaries, it was found that in spite of the low level of contaminants in the sediments of the Mondego Estuary, aquatic vegetation functioned as a source of metals for nearby systems. © CSIRO 2013.


Ngo D.-H.,Pukyong National University | Qian Z.-J.,Marine Life Research | Vo T.-S.,Pukyong National University | Ryu B.,Pukyong National University | And 2 more authors.
Carbohydrate Polymers | Year: 2011

In this research, a novel derivative of chitooligosaccharides (COS) was synthesized by covalently linking gallic acid and COS (gallate-COS) via carbodiimide to improve cellular antioxidant activity. The direct intracellular radical scavenging effect of gallate-COS by 2′,7′-dichlorofluorescin diacetate (DCFH-DA) method, and the inhibition of oxidation of cellular macromolecules, such as DNA, protein and lipid in mouse macrophages (RAW264.7 cells) were determined. Furthermore, with the treatment of gallate-COS, the expression levels of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione (GSH) were significantly increased according to reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis. In addition, gallate-COS decreased reactive oxygen species induced activation of the nuclear transcription factor (NF-κB). Collectively, these results suggest that gallate-COS can be used as a potential natural compound-based antioxidant in the functional food and pharmaceutical industries. © 2011 Elsevier Ltd. All rights reserved.


Kang K.-H.,Pukyong National University | Qian Z.-J.,Marine Life Research | Ryu B.,Pukyong National University | Kim D.,Korea Basic Science Institute | Kim S.-K.,Pukyong National University
Food Chemistry | Year: 2012

Ethanol is independently known to cause tissue damage through various mechanisms. This study was designed to evaluate the protective effect of marine microalgae, Navicula incerta protein enzymatic hydrolysates (NEHs) against ethanol-induced hepatotoxicity in HepG2 cells transfected with human CYP2E1. Induction of CYP2E1 by ethanol is one of the central pathways by which ethanol generates a state of oxidative stress in hepatocytes. When the alcohol-induced cells were treated with NEHs at various concentrations, there was a dose-dependent decrease of gamma-glutamyl transpeptidase (GGT) activity in the culture media and loss of cell viability. Among the NEHs constituents the hydrolysates which were obtained by papain (P-NEH), pronase-E (PR-NEH) and α-chymotrypsin (A-NEH) activity attenuated the ethanol cytotoxicity effectively, respectively. The activity appeared to be a GGT inhibitor. Therefore, the cytoprotective effects at alcohol-induced HepG2/CYP2E1 cells could be attributed to the inhibition of GGT activity by NEHs. This study suggests that NEHs have enough potential to be considered as highly active compounds against ethanol toxicity which leads NEHs to be significant nutraceuticals. © 2011 Elsevier Ltd. All rights reserved.


Ryu B.,Pukyong National University | Kang K.-H.,Pukyong National University | Ngo D.-H.,Pukyong National University | Qian Z.-J.,Marine Life Research | Kim S.-K.,Pukyong National University
Bioresource Technology | Year: 2012

In this study, sequential strategy based design was applied to optimize the microalgae, Pavlova lutheri mass culture conditions and fermentation conditions of the cultured algae by proteolytic yeast Candidia rugopelliculosa to obtain small peptide chains. This optimization of culture and fermentation conditions by response surface methodology (RSM) finally leads to effective purification of a bioactive peptide MPGPLSPL (793.01. Da) with hydroxyl radical scavenging activity. Collectively, these results indicated that microalgae P. lutheri can enhance the hydroxyl radical inhibiting effect through protein hydrolysis process under RSM optimal condition. © 2011 Elsevier Ltd.


Qian Z.-J.,Marine Life Research | Kang K.-H.,Pukyong National University | Kim S.-K.,Pukyong National University
Biotechnology and Bioprocess Engineering | Year: 2012

Seahorse (Hippocampus Kuda Bleeler) has been used as traditional medicine for thousands of years, in Eastern Asia. In this study of the methanol extract of fresh Hippocampus Kuda, the new compounds 2-ethyldecyl 2- ethylundecyl phthalate (1), 2, 12-diethyl-11-methylhexadecyl 2-ethyl-11- methylhexadecylphthalate (2), along with a known Bis(2-ethylheptyl) phthalate (3) were isolated. They were tested for their antioxidant activities, including lipid peroxidation inhibitory activity, DPPH radical scavenging, hydroxyl radical scavenging, superoxide anion radical scavenging, alkyl radical scavenging, and cellular radicals; these can be detected using a fluorescence probe, 2',7'- dichlorofluorescin diacetate (DCFH-DA), which could be converted to highly fluorescent dichlorofluorescein (DCF) with the presence of intracellular ROS on mouse macrophages, RAW264.7 cell. Compound (2) exhibited the highest antioxidant activity and inhibitory intracellular ROS than another compounds (1, 3). Furthermore, MTT assay showed no cytotoxicity on mouse macrophages cell (RAW264.7) and human fetal lung fibroblast cell line (MRC-5). This antioxidant property depends on concentration and increasing with increased amount of the compound. © The Korean Society for Biotechnology and Bioengineering and Springer 2012.


Kang K.-H.,Pukyong National University | Ryu B.,Pukyong National University | Kim S.-K.,Pukyong National University | Qian Z.-J.,Marine Life Research
Food Science and Biotechnology | Year: 2011

Microalgae are major primary producers of organic matters in aquatic environments through their photosynthetic activities. Benthic diatom Navicula incerta is the major component of phytoplankton and also relatively easy to cultivate, used as live food source in aquaculture. The growth characteristics of N. incerta were estimated under combinations of temperature, salinity, and light; and also its composition and antioxidant activities were determined. The maximum cell density of 87×105cells/ mL, was reached at 20°C, 250 μmol/m2·sec, 33‰ salinity, pH 8.3, 12:12 light:dark, and F/2 medium on 2 weeks of the culture period. The antioxidant enzymatic hydrolysates efficiently quenched different free radicals: 1,1-diphenyl-2- pycryl-hydrazyl (DPPH) (pepsin IC50=196.0 μg/mL), hydroxyl (α-chymotrypsin IC50=102.0 μg/mL), and superoxide (neutrase IC50=169.0 μg/mL). These results suggest that the enzymatic hydrolysate from N. incerta acts as a candidate against antioxidant and could be used as a potential functional food ingredient. © KoSFoST and Springer 2011.


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