Marine Fisheries Research Institute of Shandong Province

Yantai, China

Marine Fisheries Research Institute of Shandong Province

Yantai, China
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Xu Y.-J.,Marine Fisheries Research Institute of Shandong Province | Xu Y.-J.,Shandong Province Key Laboratory of Restoration for Marine Ecology | Tian X.-H.,Marine Fisheries Research Institute of Shandong Province | Tian X.-H.,Shandong Province Key Laboratory of Restoration for Marine Ecology | And 11 more authors.
Journal of Chromatographic Science | Year: 2012

This work describes solid-phase extraction-ultra-performance liquid chromatography with electrospray ionization tandem spectrometry for determination of malachite green and metabolite leucomalachite green, crystal violet and metabolite leucocrystal violet, methylene blue and metabolites including azure A, azure B and azure C in aquatic products. Samples were extracted with acetonitrile and ammonium acetate buffer and purified by liquid extraction with dichloromethane, and then on MCAX solid-phase extraction cartridges. Then the extract was evaporated at 458C by nitrogen blow. The residue was dissolved and separated by an Acquity BEH C18 column. The mobile phase was acetonitrile (A) and 5 mmol/L of ammonium acetate containing 0.1% formic acid (B). Analytes were confirmed and quantified using a tandem mass spectrometry system in multiple reaction mode with triple quadrupole analyzer using positive polarity mode. The limits of detection of malachite green, leucomalachite green, crystal violet and leucocrystal violet were 0.15 μg/kg, the limits of quantification were 0.50 μg/kg, and the average recoveries were more than 75% with spiked residues from 0.5 to 10 μg/kg. The relative standard deviations were less than 13%. The limits of detection of methylene blue, azure A, azure B and azure C were 0.3 μg/kg, the limits of quantification were 1.0 μg/kg, the average recoveries were more than 70% with spiked residues from 1.0 to 10 μg/kg and the relative standard deviations were less than 15%. The method has the merits of simplicity, sensitivity and rapidity, and can be used for simultaneous determination of the analytes in aquatic products. © The Author [2012]. Published by Oxford University Press. All rights reserved.


Xu Y.,Marine Fisheries Research Institute of Shandong Province | Xu Y.,Shandong Province Key Laboratory of Restoration for Marine Ecology | Tian X.,Marine Fisheries Research Institute of Shandong Province | Tian X.,Shandong Province Key Laboratory of Restoration for Marine Ecology | And 11 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2012

A rapid and simple method for the determination of colistin A and B in fishery products by reversed phase ultra performance liquid chromatography with positive electrospray ionization tandem spectrometry (UPLC-ESI-MS/MS) method was described. The samples were extracted with 1.0mol/L of hydrochloric acid in methanol-water and then purified on the PLS solid phase extraction columns. Then the eluate was evaporated to less than 1mL under a gentle stream of nitrogen at 40°C and formic acid-acetonitrile-water (0.2/10/90, v/v/v) was added to adjust volume to 1mL final volume. An aliquot (10μL) was injected onto the LC column for analysis with the mobile phase of 0.2% formic acid in acetonitrile and 0.2% formic acid in water at 0.20mLmin -1. Multiple reaction monitoring was performed using precursor-product ion combinations. Calibration curves were linear from 200ng/mL to 2000ng/mL for colistin A and B. Mean recoveries were between 72.9% and 82.9%. The LOD was 10.0μg/kg and LOQ was 40.0μg/kg. The intra-day assay precision values for QC samples were between 2.17% and 9.00%, and inter-day values were between 2.80% and 6.97%. The method has merits of simplicity, sensitivity and rapidity, and it can be used for the determination of colistin A and B in fishery products. © 2012.


Ren C.-B.,Marine Fisheries Research Institute of Shandong Province | Ren C.-B.,Yantai Shanshui Seafood Co. | Tian X.-H.,Marine Fisheries Research Institute of Shandong Province | Tian X.-H.,Shandong Province Key Laboratory of Restoration for Marine Ecology | And 10 more authors.
Journal of Chinese Mass Spectrometry Society | Year: 2013

The triazine herbicides in seawater were determined by solid phase extraction ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry(UPLC-ESI-MS/MS). UPLC and MS/MS analytical conditions were examined and optimized critically by a series of experiments. Samples were filtrated and purified on the HLB solid phase extraction cartridges. The elution was collected and evaporated to less than 1 mL at 40°C by nitrogen blow, then added to 1 mL acetonitrile and water (1:1, V/V) and ultrasonic wave extraction for 1 min. After filtrated with 0.22 μm filter membrane, the residue was separated by Acquity™ UPLC HSS C18 column (2.1 mm×100 mm×1.8 μm) using gradient elution separation. The mobile phase was a mixture of A (acetonitrile) and B (5 mmol/L ammonium acetate containing 0.1% formic acid) in a flow rate of 0.25 mL/min. Finally analytes were confirmed and quantified using MS/MS system in multiple reaction mode with triple quadrapole analyzer using positive polarity mode. The analytes show good linearity in the range of 1 ng/L to 50 ng/L with correlation coefficient from 0.992 to 0.999. The detection limit is 1 ng/L, and quantification limit is 2 ng/L. The average recoveries at three dose levels(2 ng/L, 5 ng/L and 10 ng/L) are 65.8%-103% with relative standard deviations from 4.84% to 15.2%. In addition, the method has merits of simplicity, sensitivity and rapidity, and can be used for simultaneous determination of triazine herbicides in seawater.


Du R.,Yantai University | Wang Y.,Marine Culture Institute of Shandong Province | Jiang H.,Marine Fisheries Research Institute of Shandong Province | Liu L.,Yantai University | And 4 more authors.
Chinese Journal of Oceanology and Limnology | Year: 2010

Broodstock of Verasper moseri (Jordan and Gilbert) aged 3-4 years old were selected, and reinforced cultivation was conducted to promote maturation under controlled water temperature and photoperiod conditions. Fertilized eggs were obtained by artificial fertilization, and the development of embryos, larvae and juveniles was observed continuously. The results showed that the fertilized eggs of V. moseri were spherical, with transparent yolk and homogeneous bioplasm, and had no oil globule inside. The average diameter of the eggs was 1. 77±0. 02 mm. The eggs of V. moseri were buoyant in water with salinity above 35. The cleavage type was typical discoidal. Young pigment cells appeared when olfactory plates began to form. Hatching occurred at 187 h after fertilization at a water temperature of 8. 5°C. The newly hatched larvae, floating on the water surface, were transparent with an average total length of 4. 69±0. 15 mm. During the cultivation period, when the water temperature was raised from 9 to 14. 5°C, 4-day old larvae showed more melanophores on the body surface, making the larvae gray in color. The pectoral fins began to develop, which enabled the larvae to swim horizontally and in a lively manner. On days 7-8, the digestive duct formed. The yolk sac was small and black. The yolk sac was absorbed on day 11. Larvae took food actively, and body length and body height clearly increased. The rudiments of dorsal and anal fin pterygiophores were discernible and caudal fin ray elements formed on day 19. On day 24, the larval notochord flexed upwards, and the rays of unpaired fins began to differentiate. Pigment cells converged on the dorsal and anal fin rays, and the mastoid teeth on the mandible appeared. On day 29, the left eyes of juveniles began to move upwards. Depigmentation began in some juveniles and they became sandy brown in color on day 37. Most juveniles began to settle on the bottom of the tank. The left eyes of juveniles migrated completely to the right side on day 50, when the average body length attained 2. 5±0. 18 cm, and juveniles accomplished metamorphosis to young. The embryonic and larval characters of several flounder species are compared. © 2010 Chinese Society for Oceanology and Limnology, Science Press and Springer Berlin Heidelberg.


Xu Y.,Marine Fisheries Research Institute of Shandong Province | Geng J.,Yantai Entry exit Inspection and Quarantine Bureau | Zhang X.,Marine Fisheries Research Institute of Shandong Province | Zhang S.,Marine Fisheries Research Institute of Shandong Province | And 2 more authors.
Chinese Journal of Chemistry | Year: 2010

A sensitive method for the determination of diethylstilbestrol (DES) in aquatic products by high performance liquid chromatography (HPLC) using a fluorescence detector (FLD) was developed. By means of sulfonation in concentrated sulfuric acid and hydrolysis, DES was converted into a reaction product that can emit fluorescence and determined by HPLC. For the first time, HPLC with FLD was applied to the determination of DES and sensitivity comparable to that of LC-MS gained. The limit of detection was 0.1 μg•kg-1, and the recoveries were above 86% with relative standard deviations less than 6.2%. The proposed method was successfully applied to the determination of DES in aquatic products. © 2010 SIOC, CAS, Shanghai, & WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Ma J.,Zhejiang University | Ma J.,Marine Fisheries Research Institute of Shandong Province | Shao Q.,Zhejiang University | Xu Z.,Zhejiang University | Zhou F.,Zhejiang Fisheries Technical Extension Station
Journal of the World Aquaculture Society | Year: 2013

An 8-wk feeding trial was conducted to investigate the effects of dietary n-3 highly unsaturated fatty acids (n-3 HUFA) on growth, body composition, and fatty acid profiles of juvenile Acanthopagrus schlegeli. Three replicate groups of fish (initial mean weight: 8.08±0.09g, mean ± SD) were fed diets with different levels of n-3 HUFA (0.76%, HUFA0.76; 0.83%, HUFA0.83; 0.90%, HUFA0.90; 0.97%, HUFA0.97; 1.04%, HUFA1.04; 1.12%, HUFA1.12) at 12.9% of total lipid, with a constant eicosapentaenoic acid (EPA, 20:5n-3) to docosahexaenoic acid (DHA, 22:6n-3) ratio of about 2.1. Hepatosomatic index (HSI) and intraperitoneal fat (IPF) ratio were all linearly depressed by dietary n-3 HUFA levels (P < 0.05), and condition factor (CF) was not affected. Adipocyte diameter in IPF decreased with the elevation of dietary n-3 HUFA and significance occurred between group HUFA0.90 and HUFA1.12. Lipid content in dorsal muscle was significantly lowered by dietary n-3 HUFA compared to fish fed diet HUFA0.76. No significance was found in whole fish proximate composition. In liver, dorsal muscle and IPF, ∑SFA, 16:0 or ∑n-3 HUFA were all positively correlated with dietary n-3 HUFA, while DHA to EPA ratios remained constant in 2.68, 2.86, and 3.60, respectively. Fatty acid synthase (FAS, EC 2.3.1.85) activities of all treatments remained constant at first and then were significantly elevated by dietary n-3 HUFA higher than 0.97% (P < 0.05). In contrast, hormone sensitive lipase (HSL, EC 3.1.1.3) changed following an opposite tendency. Quadratic analysis based on weight gain rate (WGR) indicated that dietary n-3 HUFA requirement for juvenile black seabream was 0.94% of the diet in 12.9% lipid diets. © Copyright by the World Aquaculture Society 2013.


Ding L.,Shanghai Ocean University | Ding L.,Marine Fisheries Research Institute of Shandong Province | Zhang L.,Marine Fisheries Research Institute of Shandong Province | Wang J.,Marine Fisheries Research Institute of Shandong Province | And 8 more authors.
Aquaculture Research | Year: 2010

A study was conducted to determine the effect of dietary lipid level on the growth performance, feed utilization, body composition and blood chemistry of juvenile starry flounder. Five isonitrogenous diets with increasing dietary lipid levels (6%, 10%, 14%, 18% and 22% dry material) were each fed to triplicate groups of starry flounder (29.9 g) for 8 weeks. Weight gain (WG) and specific growth rate of fish fed the 6% lipid diet were significantly lower than the other groups, while there was no significant difference in fish fed the 10%, 14%, 18% and 22% lipid diets. Body lipid content increased with increasing dietary lipid levels. The moisture content of the whole body was negatively correlated to the dietary lipid level. The dietary lipid level also affected the lipid content of the dorsal muscle positively. Liver lipid content increased as the dietary lipid level increased from 6% to 14% and then decreased. With increasing dietary lipid level, the nitrogen retention achieved the highest value when the fish were fed the 14% lipid diet, but there were no significant differences with the 10% and 22% groups. The plasma total protein content first showed an increasing and then a decreasing trend with increasing dietary lipid level, and it was significantly higher in the 14% lipid group than other groups. Based on the WG response using the broken-line model, the optimum dietary lipid level for juvenile starry flounder was estimated to be 10.62% in the experiment. © 2009 The Authors. Aquaculture Research © 2009 Blackwell Publishing Ltd.


Dong Z.,CAS Yantai Institute of Coastal Zone Research | Liu D.,CAS Yantai Institute of Coastal Zone Research | Wang Y.,CAS Yantai Institute of Coastal Zone Research | Di B.,CAS Yantai Institute of Coastal Zone Research | And 2 more authors.
Aquatic Ecosystem Health and Management | Year: 2012

In recent years, an increasing number of reports on blooms of Moon Jellyfish (Aurelia aurita) have occurred in the northern coast of China. Throughout the summer of 2009, we studied the occurrence of an A. aurita bloom in relation to environmental variables in the Yantai Sishili Bay of the Northern Yellow Sea. The mean abundance of A. aurita was 0.62 no m-3 in summer 2009 with a highest density of 2.28 n m-3, while the mean biomass of A. aurita was 163.7 mg C m-3. Highest biomass peaked at 673.6 mg C m-3. The present study showed that the spatial distribution of A. aurita seemed unrelated to the environmental variables: sea surface temperature, salinity, dissolved oxygen and nutrients. Chlorophyll a concentration was positively correlated to the occurrence of A. aurita in summer 2009, suggesting a cascading effect resulting from the jellyfish grazing on zooplankton that in turn reduced grazing of zooplankton on phytoplankton. Increased suitable settlement and reduced currents in the Bay by intense building of coastal construction and aquaculture rafts were discussed as possibly being the main drivers for the proliferation of Moon Jellyfish A. aurita. Further investigations on A. aurita polyps in situ should be conducted to address this hypothesis and relate these to current management policies. © 2012 Copyright Taylor and Francis Group, LLC.


Tian X.-H.,Marine Fisheries Research Institute of Shandong Province | Gong X.-H.,Marine Fisheries Research Institute of Shandong Province | Xu Y.-J.,Marine Fisheries Research Institute of Shandong Province | Ren C.-B.,Yantai Shanshui Seafood Co. | And 4 more authors.
Modern Food Science and Technology | Year: 2013

Accumulation of Prometryn in Apostichopus japonicus under laboratory conditions was studied using ultra performance liquid chromatogram tandem mass spectrometry. Apostichopus japonicus was divided randomly, and then exposed to water containing 1.00 μg/L, 10.00 μg/L and 200.00 μg/L Prometryn at 15.0±1.0°C. After exposure, apostichopus japonicus was placed into clean water for the subsequent elimination experiment. The accumulation amount increased with the increase of Prometryn concentration, presenting significantly positive correlation. The highest concentrations (Cmax) of enriched Prometryn for the three concentrations were 4.70 μg/kg, 43.80 μg/kg and 674.00 μg/kg when placed for 1 d, 3 d and 4 d, and the biggest bioconcentration factors (BCF) were 4.70, 4.38 and 3.37. BCF decreased with the increase of the Prometryn concentration, presenting significantly inverse correlation. When Apostichopus japonicus was bred in clean water, Prometryn accumulated was eliminated rapidly. Prometryn was no detected at 2 d. Dtermination performed for 14 times from 7 h to 46 d, showed 23.70±3.07 μg/kg of Prometryn in Apostichopus japonicus. The accumulation and elimination curves of Prometryn in Apostichopus japonicus provided useful information for decontamination of aquatic organisms that have Prometryn contamination.


PubMed | Marine Fisheries Research Institute of Shandong Province
Type: | Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences | Year: 2012

A rapid and simple method for the determination of colistin A and B in fishery products by reversed phase ultra performance liquid chromatography with positive electrospray ionization tandem spectrometry (UPLC-ESI-MS/MS) method was described. The samples were extracted with 1.0 mol/L of hydrochloric acid in methanol-water and then purified on the PLS solid phase extraction columns. Then the eluate was evaporated to less than 1 mL under a gentle stream of nitrogen at 40 C and formic acid-acetonitrile-water (0.2/10/90, v/v/v) was added to adjust volume to 1 mL final volume. An aliquot (10 L) was injected onto the LC column for analysis with the mobile phase of 0.2% formic acid in acetonitrile and 0.2% formic acid in water at 0.20 mL min. Multiple reaction monitoring was performed using precursor-product ion combinations. Calibration curves were linear from 200 ng/mL to 2000 ng/mL for colistin A and B. Mean recoveries were between 72.9% and 82.9%. The LOD was 10.0 g/kg and LOQ was 40.0 g/kg. The intra-day assay precision values for QC samples were between 2.17% and 9.00%, and inter-day values were between 2.80% and 6.97%. The method has merits of simplicity, sensitivity and rapidity, and it can be used for the determination of colistin A and B in fishery products.

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