Ladhar-Chaabouni R.,Marine Ecotoxicology |
Machreki-Ajmi M.,Marine Ecotoxicology |
Serpentini A.,University of Caen Lower Normandy |
Lebel J.-M.,University of Caen Lower Normandy |
Hamza-Chaffai A.,Marine Ecotoxicology
Environmental Science and Pollution Research | Year: 2014
In this study, a model based on primary cultured haemocytes from the gastropod mollusc Haliotis tuberculata was established to investigate the effects of cadmium chloride in vitro. Cells were exposed for 24 h to CdCl2 concentrations of 0, 1 and 100 μg ml-1. The effects of cadmium on haemocyte parameters were investigated using morphological, spectrophotometric and flow cytometry analysis. Results showed that cadmium has no significant effects on cell viability and phagocytotic activity under the tested conditions. However, haemocytes became more rounded after cadmium exposure, which could explain the significant decrease of cell area beginning at 1 μg ml-1 of CdCl2. © 2014 Springer-Verlag Berlin Heidelberg.