Marine Chemical Research Institute

Qingdao, China

Marine Chemical Research Institute

Qingdao, China

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Ma D.,Shandong University | Wu L.,Marine Chemical Research Institute | Feng S.,Shandong University | Lu H.,Shandong University | Zhao S.,Shandong University
Radiation Physics and Chemistry | Year: 2017

Nanoscale Yttrium phosphate (YPO4) particles was prepared by hydrothermal method, and was used as anti-radiation additive for methyl vinyl silicone rubber (MVQ rubber). The mechanical properties and crosslink density of YPO4/MVQ rubber nanocomposites before and after irradiated by γ-rays were determined. The irradiation protection mechanism of YPO4 was discussed using the fluorescence spectra. The results showed that YPO4 could improve the irradiation-resistant properties of silicone rubber. © 2017 Elsevier Ltd


Wang Z.,Hubei University | Wang Z.,Marine Chemical Research Institute | Qi Y.,Hubei University | Qi H.,Qingdao University | And 2 more authors.
Journal of Materials Science: Materials in Electronics | Year: 2010

Hollow and hierarchical Bi 3.15Nd 0.85Ti 3O 12 (BNdT) microspheres of 0.5-1.2 lm in diameter were synthesized through a hydrothermal process. Each hollow microsphere is constructed across by many single-crystalline BNdT nanoplates with in-plane dimension of ∼400 nm. The BNdT nanoplates are of layered perovskites. The UV-visible absorption characteristics demonstrated that the band gap of the BNdT microspheres is 3.33 eV. The hierarchical microspheres exhibit significant photocatalytic activity. Up to 75% methyl orange was decolorized after UV irradiation for 210 min, whereas lower than 10% methyl orange decolorized using BNdT powders prepared from single crystals as catalyst. The photocatalytic decolorization of methyl orange solution is a pseudo-first-order reaction and its kinetics can be expressed as ln(C/C 0) = kt. © Springer Science+Business Media, LLC 2010.


Sun H.-Y.,Ocean University of China | Wang K.,Marine Chemical Research Institute | Chi Z.,Ocean University of China | Xu H.-M.,Ocean University of China | Chi Z.-M.,Ocean University of China
Process Biochemistry | Year: 2012

The yeast Wickerhamomyces anomalus (the previous name was Pichia anomala) HN1-2 isolated from the mangrove ecosystem was found to be able to produce high level of both killer toxin and single cell protein. When the killer yeast cells were grown by batch cultivation in 5-l fermentor, crude protein in the cells, cell mass, reducing sugar, and diameter of the inhibition zone reached 56.0 g per 100 g of cell dry weight, 7.3 g per liter, 9.5 g per liter, and 19.0 mm, respectively within 12 h and this yeast synthesized a large amount of the essential amino acids, such as lysine (7.8%), methionine (1.8%), and leucine (9.0%). The crude killer toxin produced by the killer yeast isolate HN1-2 could kill the cells of Lodderomyces elongisporus, Candida albicans, Metschnikowia bicuspidata, Pichia guilliermondii, Saccharomyces cerevisiae, Yarrowia lipolytica, and Kluyveromyces aestuarii, which were widely distributed in natural marine environments. The results also showed that the undesirable yeast could be avoided during cell growth of the killer yeast. © 2011 Elsevier Ltd. All rights reserved.


Liu G.-L.,Ocean University of China | Wang K.,Marine Chemical Research Institute | Hua M.-X.,Ocean University of China | Buzdar M.A.,Ocean University of China | Chi Z.-M.,Ocean University of China
Process Biochemistry | Year: 2012

The psychrotolerant yeast Mrakia frigida 2E00797 isolated from sea sediments in Antarctica was found to be able to produce killer toxin against Metschnikowia bicuspidata, Candida tropicalis and Candida albicans. In the present study, the killer toxin was purified and characterized. The molecular weight of the purified killer toxin was estimated to be 55.6 kDa and the purified killer toxin shared 35.1% sequence homology with a protein kinase. The purified killer toxin's optimal temperature and pH for killing activity were 16 °C and 4.5, respectively, and it was stable in the temperature range from 10 to 25 °C at pH 4.5. The toxin's highest killing activity was observed in the presence of 3.0 g/100 ml NaCl. The purified killer toxin was able to actively kill whole cells of M. bicuspidata but could not kill the protoplast of the sensitive yeast. Of the eight yeast species tested in this study, the killer toxin was able to kill C. tropicalis and C. albicans in addition to M. bicuspidata. © 2012 Elsevier Ltd. All rights reserved.


Ding R.,Marine Chemical Research Institute | Jiang J.,Marine Chemical Research Institute | Gui T.,Marine Chemical Research Institute
Journal of Coatings Technology Research | Year: 2016

This paper studies the evolution of impedance model of 30 μm solvent-free epoxy coating on Q235 steel surface in 3.5% NaCl solution using electrochemical impedance spectroscopy. The electrochemical process of the system was divided into five stages. Water absorption occurred at the coating mainly during the early stage of immersion. In the meantime, coating resistance became smaller and coating impedance deviated from purely capacitive characteristics. After water permeated into the metal/coating interface, corrosion reaction began. During the middle stage of immersion, due to the barrier effect of the coating, the electrochemical reaction at the coating/metal interface was controlled by diffusion of corrosion products. Since the coating prepared was relatively thin, transport distance was short for water, which could easily reach the coating/metal interface. During the middle-late stage of immersion, coating peeled off from the metal surface, diffusion of water molecules gradually turned to a macroscopic infiltration process, and the coating lost its protective effect. A logarithm of coating capacitance and the square root of time showed a linear relationship in the early immersion stage, which was a typical characteristic of Fick’s diffusion. Water diffusion coefficient in the coating was calculated to be 8.23 × 10−11 cm2/s, while volume fraction and total water absorption at saturation of coating were 3.5% and 105 μg/cm2, respectively, indicating good water resistance and protective properties of the coating. By solving the Fick’s diffusion equation, the kinetic equation which described the diffusion of water in the coating and included time and location variables were obtained. © 2016 American Coatings Association


Jiao C.,Qingdao University of Science and Technology | Zhuo J.,Qingdao University of Science and Technology | Chen X.,Qingdao University of Science and Technology | Li S.,Qingdao University of Science and Technology | Wang H.,Marine Chemical Research Institute
Journal of Thermal Analysis and Calorimetry | Year: 2013

Flame retardant epoxy resin (FREP) were synthesized from phosphoric acid and bisphenol A epoxy resin (BAEP). The structure of the FREP was characterized using FTIR and 31P NMR. Then, several FREP/BAEP mass ratios were used to obtain materials with different phosphorus contents. The properties of the thermosetting materials were evaluated by limiting oxygen index, UL 94, cone calorimeter test, and thermogravimetric analysis. The results showed that the heat release rates and smoke production rates decreased greatly, and char residue increased with the increasing of FREP. It indicates that good flame retardant properties are related to the formation of a protective phosphorus-rich char layer. © 2012 Akadémiai Kiadó, Budapest, Hungary.


Sun H.H.,Marine Chemical Research Institute | Mo X.M.,Qingdao women and Children Hospital | Qian Y.M.,Marine Chemical Research Institute
Advanced Materials Research | Year: 2013

To explore and compare the reliability of the quantitative determination of formaldehyde content of water based coatings, the high performance liquid chromatography (HPLC) after derivatization to 2.4-dinitrophenylhydazone and UV-VIS spectrophotometry were studied, respectively. HPLC was performed on a C18 column with acetonitrile/water (65/35, V/V) as mobile phase at a flow rate of 1.0 mL/min, and analyses was detected using Photodode Array Detector (DAD) at the wavelength of 360 nm. And formaldehyde was determined with spectrophotometry at 435nm. The result showed the standard curve of formaldehyde by the HPLC method was linear in the range of 0.1-5.0 mg/L (R2=0.9998). The detection limit was 0.96mg/kg, recoveries were ranging from 96.2% to 101.3%. The linear range of formaldehyde by the spectrophotometric method was 0.1~5.0 mg/L (R2=0.9995), and the detection limit was 5.93mg/kg, recoveries were ranging from 98.5% to 105.3%. In conclusion, both the two methods can be used for quantitative analysis of formaldehyde in water based coatings, and the major advantages of HPLC are quick, simple and sensitive. © (2013) Trans Tech Publications, Switzerland.


Ding R.,Marine Chemical Research Institute | Cong W.-W.,Marine Chemical Research Institute | Jiang J.-M.,Marine Chemical Research Institute | Gui T.-J.,Marine Chemical Research Institute
Journal of Coatings Technology Research | Year: 2016

This work studied the evolution of an impedance model of 60 μm modified solvent-free epoxy anti-corrosion coating on a Q235 steel surface in 3.5% NaCl solution using electrochemical impedance spectroscopy. The electrochemical process of the system was divided into five stages. During the early stage of immersion, water absorption occurred mainly at the coating, coating resistance decreased, and coating impedance deviated from purely capacitive characteristics. The corrosion reaction started after water permeated into the metal/coating interface. During the mid-immersion stage, the electrochemical reaction at the coating/metal interface was controlled by semi-infinite diffusion of corrosion products due to the barrier effect of the coating. The types of corrosion product diffusion gradually became finite layer diffusion and barrier layer diffusion with the clogging of coating pores. Logarithm of coating capacitance and the square root of time showed a linear relationship in the early immersion stage, which was a typical characteristic of Fick’s diffusion. Afterwards, increase in the coating capacitance slowed down, and a non-Fickian diffusion process occurred, which presented the two-stage absorption characteristics. Water diffusion coefficient in coating was calculated to be 2.95 × 10−10 cm2/s, while volume fraction and total water absorption at saturation of coating were 2.3% and 185 μg, respectively, indicating good water resistance and protective properties of the coating. The water kinetic equation in the coating which can reflect the whole Fick diffusion process of water and containing time and location variables was obtained via the Fick diffusion equation and three-dimensional images of water distribution in the coating were drawn by transforming the dynamic equation into programs. © 2016 American Coatings Association


Gao M.,Ocean University of China | Su R.,Ocean University of China | Wang K.,Marine Chemical Research Institute | Li X.,Marine Chemical Research Institute | Lu W.,Marine Chemical Research Institute
Marine Pollution Bulletin | Year: 2013

A fungus, Aureobasidium pullulans, was isolated from marine biofilm and identified. A bioassay-guided fractionation procedure was developed to isolate and purify antifouling compounds from A. pullulans HN. The procedure was: fermentation broth-aeration and addition of sodium thiosulfate-graduated pH and liquid-liquid extraction-SPE purification-GC-MS analysis. Firstly, the fermentation broth was tested for its toxicity. Then it was treated with aeration and addition of sodium thiosulfate, and its toxicity was almost not changed. Lastly, antifouling compounds were extracted at different pH, the extract had high toxicity at pH 2 but almost no toxicity at pH 10, which suggested the toxicants should be fatty acids. The EC50 of the extract against Skeletonema costatum was 90.9μgml-1, and its LC50 against Balanus amphitrete larvae was 22.2μgml-1. After purified by HLB SPE column, the EC50 of the extract against S. costatum was 49.4μgml-1. The myristic and palmitic acids were found as the main toxicants by GC-MS. © 2013 Elsevier Ltd.


Li Y.,Ocean University of China | Liu G.-L.,Ocean University of China | Wang K.,Marine Chemical Research Institute | Chi Z.-M.,Ocean University of China | Madzak C.,Ocean University of China
Journal of Molecular Catalysis B: Enzymatic | Year: 2012

The endo-inulinase gene (EnIA) from Arthrobacter sp. S37 was ligated into the expression vector pINA1317 and over-expressed in Yarrowia lipolytica Po1h. It was found that the endo-inulinase activity and specific endo-inulinase activity produced by the transformant 1317-EnIA were 16.7 U/mL and 93.4 U/mg, respectively. The recombinant EnIA was purified and characterized. The molecular weight of the purified rEnIA was 78.9 kDa. The optimal pH and temperature of the purified rEnIA were 4 and 50 °C, respectively. The purified rEnIA was stable in the temperature range of 4-40 °C and in the pH range of 2-8. The activity of rEnIA was greatly stimulated in the presence of Li+. The purified rEnIA could actively convert inulin into disaccharides. .© 2011 Elsevier B.V. All rights reserved.

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