Marine Biotechnology Institute Co.

Shizuoka-shi, Japan

Marine Biotechnology Institute Co.

Shizuoka-shi, Japan

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Yoon J.,Keimyung University | Jang J.-H.,Marine Biotechnology Institute Co. | Jang J.-H.,Korea Research Institute of Bioscience and Biotechnology | Kasai H.,Marine Biotechnology Institute Co. | Kasai H.,Kitasato University
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2013

A Gram-negative, strictly aerobic, golden-yellow pigmented, non-motile, rod-shaped strain designated J36A-7T was isolated from an unidentified yellow-green coloured marine sponge. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Flavobacteriaceae of the phylum Bacteroidetes and that it showed highest sequence similarity (85.6 %) to Nonlabens dokdonensis DSW-6T. The strain could be differentiated phenotypically from recognized members of the family Flavobacteriaceae. The major fatty acids of strain J36A-7T were identified as iso-C16:0, iso-C17:0 3-OH and summed feature three as defined by the MIDI system (C16:1 ω7c and/or iso-C15:0 2-OH). The DNA G+C content was determined to be 31.2 mol%, the major respiratory quinone was identified as menaquinone 6 (MK-6) and a complex polar lipid profile was present consisting of phosphatidylethanolamine, two unidentified glycolipids, one unidentified aminolipid and one unidentified lipid. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel genus for which the name Spongiimonas flava gen. nov., sp. nov. is proposed. The type strain of S. flava is J36A-7T (=KCTC 32176T = NBRC 109319 T). © 2012 Springer Science+Business Media Dordrecht.


Yoon J.,Keimyung University | Katsuta A.,Marine Biotechnology Institute Co. | Katsuta A.,Kitasato University | Kasai H.,Marine Biotechnology Institute Co. | Kasai H.,Kitasato University
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2012

A strictly aerobic, Gram-negative, reddish-orange pigmented, non-motile and rod-shaped bacterium, designated AK17-053 T was isolated from a marine crustacean (Squillidae) living on tidal flats on the coast of the Ariake Sea, Nagasaki, Japan. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the novel isolate could be affiliated with the family Saprospiraceae of the phylum Bacteroidetes and that it showed highest sequence similarity (84%) with Lewinella marina MKG-38 T. The strain could be differentiated phenotypically from recognized members of the family Saprospiraceae. The G+C content of DNA was 55.3 mol%, MK-7 was the major menaquinone and iso-C 15:0 and C 16:1ω7c were the major fatty acids. On the basis of polyphasic taxonomic studies, it was concluded that strain AK17-053 T represents a new genus of the family Saprospiraceae. We propose the name Rubidimonas crustatorum gen. nov., sp. nov. for this strain; its type strain is AK17-053 T (= MBIC08356 T = NBRC 107717 T). © 2011 Springer Science+Business Media B.V.


Yoon J.,Keimyung University | Yoon J.,University of Tokyo | Oku N.,Marine Biotechnology Institute Co. | Oku N.,Toyama Prefectural University | And 4 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2011

A strictly aerobic, gram-negative, non-motile, reddish-pink pigmented, rod-shaped strain designated N5EA6-3A2BT, was isolated from an unidentified marine sponge by use of a bait-streaked agar technique. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the novel isolate represented a distinct and deep evolutionary lineage of descent in the family Flammeovirgaceae within the phylum Bacteroidetes and clustered with as yet uncultured bacteria. The most closely related established species was Roseivirga spongicola UST030701-084T (89% sequence similarity) in the family of Flammeovirgaceae. The strain could be differentiated phenotypically and physiologically from recognized members of the family Flammeovirgaceae. The G+C content of DNA was 43 mol%, the major respiratory quinone was menaquinone 7 (MK-7) and iso-C15:0, C16:1ω5c and iso-C17:0 3-OH were the major fatty acids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the name Porifericola rhodea gen. nov., sp. nov. is proposed. The type strain of Porifericola rhodea is N5EA6-3A2BT (=MBIC08357T = NBRC 107748T). © 2011 Springer Science+Business Media B.V.


Matsuo Y.,Suntory Holdings Ltd. | Kanoh K.,Nippon Steel & Sumitomo Metal Corporation | Jang J.-H.,Korea Research Institute of Bioscience and Biotechnology | Adachi K.,Nippon Steel Chemical Co. | And 4 more authors.
Journal of Natural Products | Year: 2011

A new catechol-type siderophore, streptobactin (1), was isolated from a culture broth of the marine-derived actinomycete Streptomyces sp. YM5-799. The structure of streptobactin was determined by NMR and MS analyses and ESIMS/MS experiments to be a cyclic trimer of benarthin. A dibenarthin (2), a tribenarthin (3), and benarthin (4) were also obtained. The production of 1 was regulated by an iron concentration in the culture. The iron-chelating activity of the compounds was evaluated by the chrome azurol sulfonate assay. © 2011 American Chemical Society.


Raju R.,University of The South Pacific | Kawabata K.,Marine Biotechnology Institute Co. | Nishijima M.,Marine Biotechnology Institute Co. | Aalbersberg W.G.L.,University of The South Pacific
Tetrahedron Letters | Year: 2012

Chemical analysis of a marine-derived Vibrio sp. (Q-459) isolated from a depth of 10 m near Okinawa prefecture Japan, yielded a new cyclic acyldepsipeptide, kailuin F (1) together with the known kailuins B (3) and E (6). The structure of 1 was elucidated based on spectroscopic methods including UV, HR-ESIMS, chemical derivatizations and 1D, 2D NMR data. Kailuin B (3) exhibited a strong antimicroalgal activity against the dinoflagellate Prorocentrum micans. © 2012 Elsevier Ltd. All rights reserved.


Matsuda T.,Kyushu University | Sakaguchi K.,Kyushu University | Kobayashi T.,Kyushu University | Abe E.,Kyushu University | And 8 more authors.
Journal of Biochemistry | Year: 2011

We isolated a putative desaturase gene from a marine alga, Pinguiochrysis pyriformis MBIC 10872, which is capable of accumulating eicosapentaenoic acid (C20:5 Δ5,8,11,14,17). The gene possessed an open reading frame of 1,314 bp encoding a putative 437 amino acid residues showing high sequence identity (37-48) with fungal and nematode Δ12-fatty acid desaturases. Yeast cells transformed with the gene converted endogenous oleic acid (C18:1 Δ9) to linoleic acid (C18:2 Δ9,12). However, no double bonds were introduced into other endogenous fatty acids or exogenously added fatty acids. Flag-tagged enzyme was recovered in the micosome fraction when expressed in yeast cells. To express the gene in thraustochytrids, a construct driven by the thraustochytrid-derived ubiquitin promoter was used. Interestingly, exogenously added oleic acid was converted to linoleic acid in the gene transformants but not mock transformants of Aurantiochytrium limacinum mh0186. These results clearly indicate that the gene encodes a microsomal Δ12-fatty acid desaturase and was expressed functionally in not only yeasts but also thraustochytrids. This is the first report describing the heterozygous expression of a fatty acid desaturase in thraustochytrids, and could facilitate a genetic approach towards fatty acid synthesis in thraustochytrids which are expected to be an alternative source of polyunsaturated fatty acids. © 2011 The Authors. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.


Yoon J.,University of Tokyo | Matsuda S.,Marine Biotechnology Institute Co. | Adachi K.,Marine Biotechnology Institute Co. | Kasai H.,Marine Biotechnology Institute Co. | Yokota A.,University of Tokyo
International Journal of Systematic and Evolutionary Microbiology | Year: 2011

A Gram-negative-staining, obligately aerobic, non-motile, rod-shaped and chemoheterotrophic bacterium, designated strain MN1-1006 T, was isolated from an ascidian (sea squirt) sample, and was studied using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the new isolate shared approximately 93-99% sequence similarity with recognized species of the genus Rubritalea within the phylum 'Verrucomicrobia'. DNA-DNA hybridization values between strain MN1-1006 T and Rubritalea squalenifaciens HOact23 T and Rubritalea sabuli YM29-052 T were 57% and 14.5%, respectively. Strain MN1-1006 T produced carotenoid compounds that rendered the cell biomass a reddish pink colour. The strain also contained squalene. The cell-wall peptidoglycan of the novel strain contained muramic acid and meso-diaminopimelic acid. The DNA G+C content of strain MN1-1006 T was 51.4 mol%. The major cellular fatty acids were iso- C 14:0, iso-C 16:0 and anteiso- C 15:0. The major isoprenoid quinone was MK-9. On the basis of these data, it was concluded that strain MN1-1006 T represents a novel species of the genus Rubritalea, for which the name Rubritalea halochordaticola sp. nov. is proposed. The type strain is MN1-1006 T (=KCTC 23186 T =NBRC 107102 T). © 2011 IUMS.

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