Marine Biology Institute of Shandong Province

Qingdao, China

Marine Biology Institute of Shandong Province

Qingdao, China
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Sun X.,Chinese Academy of Fishery Sciences | Li L.,Marine Biology Institute of Shandong Province | Wang X.,Marine Biology Institute of Shandong Province
Conservation Genetics Resources | Year: 2017

The ark shell Scapharca subcrenata, an important fishery resource in Asian countries, has been suffering from severe population decline in recent years. To facilitate genetic conservation studies, novel single nucleotide polymorphisms (SNPs) markers were developed using next generation sequencing for this species, which yielded a total of 781,860 putative SNPs. To validate these markers, 96 SNP genotyping assays were designed to characterize polymorphisms using high resolution melting (HRM) analysis in 32 individuals from a wild population. As a result, 37 SNPs showed polymorphisms with MAF ranging from 0.0313 to 0.4844. The observed heterozygosity ranged from 0 to 0.8333, while the expected heterozygosity varied from 0.0605 to 0.4995. After the Bonferroni correction, 5 of the 37 loci showed significant departure from Hardy–Weinberg equilibrium. These novel SNP markers will be useful for future investigation of population structure and conservation genetics of the ark shell S. subcrenata. © 2017, Springer Science+Business Media Dordrecht.


Xu Z.,Huaihai Institute of Technology | Xu Z.,Marine Biology Institute of Shandong Province | Gao G.,Huaihai Institute of Technology | Gao G.,Northumbria University | And 2 more authors.
Biogeosciences | Year: 2017

The development of golden tides is potentially influenced by global change factors, such as ocean acidification and eutrophication, but related studies are very scarce. In this study, we cultured a golden tide alga, Sargasssum muticum, at two levels of pCO2 (400 and 1000ĝ€μatm) and phosphate (0.5 and 40ĝ€μM) to investigate the interactive effects of elevated pCO2 and phosphate on the physiological properties of the thalli. Higher pCO2 and phosphate (P) levels alone increased the relative growth rate by 41 and 48ĝ€%, the net photosynthetic rate by 46 and 55ĝ€%, and the soluble carbohydrates by 33 and 62ĝ€%, respectively, while the combination of these two levels did not promote growth or soluble carbohydrates further. The higher levels of pCO2 and P alone also enhanced the nitrate uptake rate by 68 and 36ĝ€%, the nitrate reductase activity (NRA) by 89 and 39ĝ€%, and the soluble protein by 19 and 15ĝ€%, respectively. The nitrate uptake rate and soluble protein was further enhanced, although the nitrate reductase activity was reduced when the higher levels of pCO2 and P worked together. The higher pCO2 and higher P levels alone did not affect the dark respiration rate of the thalli, but together they increased it by 32ĝ€% compared to the condition of lower pCO2 and lower P. The neutral effect of the higher levels of pCO2 and higher P on growth and soluble carbohydrates, combined with the promoting effect on soluble protein and dark respiration, suggests that more energy was drawn from carbon assimilation to nitrogen assimilation under conditions of higher pCO2 and higher P; this is most likely to act against the higher pCO2 that caused acid-base perturbation via synthesizing H+ transport-related protein. Our results indicate that ocean acidification and eutrophication may not boost golden tide events synergistically, although each one has a promoting effect. ©The Author(s) 2017.


Liu G.,Marine Biology Institute of Shandong Province | Sun J.,CAS Qingdao Institute of Oceanology | Liu S.,CAS Qingdao Institute of Oceanology
Developments in Aquaculture and Fisheries Science | Year: 2015

In China, Apostichopus japonicus has a long history of consumption, owing mainly to the health benefits and medicinal properties attributed to sea cucumber. The traditional dive-fishing method for A. japonicus is still being used in China today. With the socioeconomic boom in the 1990s, the market demand for sea cucumber increased considerably and led to the sharp decline of natural resources. Consequently, A. japonicus aquaculture emerged and became the most important source of A. japonicus on the market. With increasing wealth and consumer demand in China, A. japonicus aquaculture is expected to expand in years to come, despite limiting factors, such as diseases, environmental deterioration, and space constraints. © 2015 Elsevier Inc.


PubMed | State Oceanic Administration, Qingdao Tongyong Aquaculture Ltd Company, Marine Biology Institute of Shandong Province and CAS Qingdao Institute of Oceanology
Type: Journal Article | Journal: Theriogenology | Year: 2016

The present study established and confirmed an efficient technology for groupers: giant grouper Epinephelus lanceolatus, orange-spotted grouper E. coioides, seven-band grouper, E. septemfasciatus, and kelp grouper E. moara sperm cryopreservation and successfully applied the cryopreserved E. lanceolatus sperm into interspecific hybridization with E. coioides. For both E. lanceolatus and E. coioides, the best motility of postthaw sperm were achieved using 6% to 10% DMSO, 6% to 16% propylene glycol, and 6% ethylene glycol as cryoprotectants. Furthermore, we have successfully applied this method into the other two species of E. septemfasciatus (74.565.45%) and E. moara (71.675.10%) sperm cryopreservation and obtained high motility, respectively. Computer-assisted sperm motion analysis showed that the postthaw sperm of the four species of grouper could keep 30 to 35minutes motile state in nature seawater. And the freezing-thawing process decreased the sperm motility, speed, and longevity but did not significantly change the sperm movement pattern, and the progressive linear motion still was the dominant movement pattern. For the four species of grouper, the ultrastructural analysis showed 70% to 80% of the spermatozoa had intact morphology with a little of swelling; 20% to 30% were damaged, such as swelling or rupture of head, midpiece, and tail region; and 10% to 20% were severely damaged. Whereas, by the microscopic observation, more than 90% of the postthaw sperm presented normal morphology. In the artificial insemination and hybridization experiment, high fertilization rates and hatching rates were achieved when using 10% DMSO (88.75.3%, 85.37.4%) and 10% propylene glycol (86.83.3%, 83.16.6%), with no significant difference in comparison with control (92.21.4%, 87.94.2%). In addition, we found the embryos from postthaw sperm of E. lanceolatus and E. coioides eggs developed and grew normally as reported in previous study on hybridization of groupers (E. coioidesE. lanceolatus) using cryopreserved sperm. The results of the present study further validated the safety of the cryopreserved sperm in breeding production by assessing the fertilization capacity, embryo development, and larval growth.


Wang L.,Nanjing University | Wang X.,Marine Biology Institute of Shandong Province | Wu H.,Nanjing University | Liu R.,Nanjing University
Marine Drugs | Year: 2014

Among the three main divisions of marine macroalgae (Chlorophyta, Phaeophyta and Rhodophyta), marine green algae are valuable sources of structurally diverse bioactive compounds and remain largely unexploited in nutraceutical and pharmaceutical areas. Recently, a great deal of interest has been developed to isolate novel sulfated polysaccharides (SPs) from marine green algae because of their numerous health beneficial effects. Green seaweeds are known to synthesize large quantities of SPs and are well established sources of these particularly interesting molecules such as ulvans from Ulva and Enteromorpha, sulfated rhamnans from Monostroma, sulfated arabinogalactans from Codium, sulfated galacotans from Caulerpa, and some special sulfated mannans from different species. These SPs exhibit many beneficial biological activities such as anticoagulant, antiviral, antioxidative, antitumor, immunomodulating, antihyperlipidemic and antihepatotoxic activities. Therefore, marine algae derived SPs have great potential for further development as healthy food and medical products. The present review focuses on SPs derived from marine green algae and presents an overview of the recent progress of determinations of their structural types and biological activities, especially their potential health benefits. © 2014 by the authors; licensee MDPI.


Zhang Q.,Ocean University of China | Liu J.,Marine Biology Institute of Shandong Province | Zhang P.-D.,Ocean University of China | Liu Y.-S.,Ocean University of China | Xu Q.,Ocean University of China
Aquatic Botany | Year: 2015

This study tested the responses of eelgrass Zostera marina transplants to changes in the silt and clay content of sediments. A transplantation experiment using the staple method was conducted in Swan Lake on the eastern coast of Shandong Peninsula, China. We subjected Z. marina to different weight percentages of silt and clay in sediments (0%, 25%, 38%, 50%, 75%, and 100%) for 120 days and then measured plant response in terms of survivorship, growth rate, individual shoot weight and productivity. Natural sediments (38% silt and clay content) from the experimental site were used as reference sediment. The survival rate of Z. marina transplants was 100%. Aboveground absolute growth rate, shoot weight, and productivity of Z. marina transplants grown on different sediment types varied significantly with maximum values recorded at 75% silt and clay content and the minimum value in reference sediment. However, the rhizome elongation rate was not significantly affected by the silt and clay content of the sediment. The results indicated that sediments with high silt and clay content could promote successful eelgrass transplantation. However, no negative responses were found on sediments with low silt and clay content. The sediment with 75% silt and clay content may be the most suitable for the growth and establishment of Z. marina transplants. © 2015 Elsevier B.V.


PubMed | Ocean University of China and Marine Biology Institute of Shandong Province
Type: Journal Article | Journal: Journal of environmental sciences (China) | Year: 2014

Perinereis aibuhitensis was used to assess adverse biological effects caused by acute and chronic Pb exposure in artificial seawater under controlled laboratory conditions. In 96-hr acute toxicity experiments, the morphological changes showed a positive time/dose-dependent tendency, and the 96-hr LC value of Pb was 686.41 mg/L. The responses of enzymatic and non-enzymatic antioxidants in tissues including catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), malondialdehyde (MDA) and the content of total soluble protein (TSP), were investigated on days 1, 4, 7 and 10 after Pb exposure under chronic toxicity testing. Results showed that the activation of the antioxidant system in P. aibuhitensis depended on the Pb concentration and the duration of exposure time. Specifically, POD and SOD activities were induced on the first day of the exposure and decreased to the control level on day 10 after exposure. Therefore, these two indexes could be used to indicate oxidative stress associated with P. aibuhitensis exposure to Pb.


PubMed | Nanjing University and Marine Biology Institute of Shandong Province
Type: Journal Article | Journal: Marine drugs | Year: 2014

Among the three main divisions of marine macroalgae (Chlorophyta, Phaeophyta and Rhodophyta), marine green algae are valuable sources of structurally diverse bioactive compounds and remain largely unexploited in nutraceutical and pharmaceutical areas. Recently, a great deal of interest has been developed to isolate novel sulfated polysaccharides (SPs) from marine green algae because of their numerous health beneficial effects. Green seaweeds are known to synthesize large quantities of SPs and are well established sources of these particularly interesting molecules such as ulvans from Ulva and Enteromorpha, sulfated rhamnans from Monostroma, sulfated arabinogalactans from Codium, sulfated galacotans from Caulerpa, and some special sulfated mannans from different species. These SPs exhibit many beneficial biological activities such as anticoagulant, antiviral, antioxidative, antitumor, immunomodulating, antihyperlipidemic and antihepatotoxic activities. Therefore, marine algae derived SPs have great potential for further development as healthy food and medical products. The present review focuses on SPs derived from marine green algae and presents an overview of the recent progress of determinations of their structural types and biological activities, especially their potential health benefits.


PubMed | Ocean University of China and Marine Biology Institute of Shandong Province
Type: Journal Article | Journal: Marine pollution bulletin | Year: 2015

We describe an innovative method of planting Zostera marina (eelgrass) seeds in which hessian bags filled with high-silted sediments are used as a seed protecting device. Here, we evaluated the effectiveness of the method through a field seed-sowing experiment over a three year period. The suitable seed planting density required by the seeds of Z. marina in this method was also investigated. In the spring following seed distribution, seedling establishment rate of Z. marina subjected to different seed densities of 200-500seedsbag(-1) ranged from 16% to 26%. New eelgrass patches from seed were fully developed and well maintained after 2-3years following distribution. The seed planting density of 400seedsbag(-1) may be the most suitable for the establishment of new eelgrass patches. Our results demonstrate that seed-based restoration can be an effective restoration tool and the technique presented should be considered for future large-scale Z. marina restoration projects.


PubMed | Marine Biology Institute of Shandong Province
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

Perinereis aibuhitensis is a commercially and ecologically important intertidal worm. In this study, eight polymorphic microsatellite loci were isolated in this species for the first time, and a wild population was used to estimate the properties of these loci. The number of alleles per locus ranged from 6 to 20, and the observed and expected heterozygosities ranged from 0.375 to 0.875 and from 0.590 to 0.946, respectively. These microsatellite loci will act as effective markers for related P. aibuhitensis studies, and the data will be helpful for the rational exploitation and conservation of this species.

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