Carrigaline, Ireland
Carrigaline, Ireland

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O'Callaghan Y.C.,University College Cork | Drummond E.,Marigot Ltd | O'Gorman D.M.,Marigot Ltd | Obrien N.M.,University College Cork
Journal of Medicinal Food | Year: 2013

A high dietary intake of polyphenols has been associated with a decreased risk of cardiovascular disease and cancer, attributed in part to their antioxidant activity and pro-Apoptotic effects. Aquamin is a multi-mineral algal extract that enhances bone mineralization, relieves osteoarthritis, and aids digestion; however, Aquamin has not demonstrated antioxidant activity. In the present study, Aquamin was supplemented with 8% Enzogenol, a pine bark extract with a high phenolic content, and 2% Sunphenon, a green tea extract that also has a high phenolic content to produce a mixed product (A:E:S). The antioxidant activity of A:E:S was compared with that of its constituent compounds and also with catechin and epigallocatechin by measuring total phenol content, ferric-reducing antioxidant potential, and 2,2-diphenyl-2-picrylhydrazyl hydrate. The cytotoxic and apoptotic effects of the compounds were also measured in the U937, human monocytic blood cell line. A:E:S demonstrated an antioxidant activity that was equal to that of the compounds used in its preparation. Aquamin was not cytotoxic in the U937 cell line; however, A:E:S was cytotoxic and the primary mechanism of cell death was apoptosis. The biological effects of Aquamin were enhanced by supplementation with Enzogenol and Sunphenon to include antioxidant effects and the ability to induce apoptosis in U937 cells. © Copyright 2013, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition 2013.


O'Gorman D.M.,Marigot Ltd | O'Carroll C.,University College Cork | O'Carroll C.,Alimentary Pharmabiotic Center | Carmody R.J.,University College Cork | Carmody R.J.,Alimentary Pharmabiotic Center
Phytotherapy Research | Year: 2012

It is well established that nuclear factor kappa B (NF-κB) is a central regulator of the immune response and that dysregulation of NF-κB contributes to the pathogenesis of many autoimmune and inflammatory diseases. The food supplement Aquamin is a natural multi-mineral derived from the red algae Lithothamnion corallioides, rich in calcium, magnesium and 72 other trace minerals. This study describes an anti-inflammatory role for Aquamin in inhibiting NF-κB activation through reducing the phosphorylation and degradation of its upstream inhibitor IκBα. Aquamin inhibition of NF-κB activation results in significantly reduced cyclo-oxygenase-2 gene expression following treatment of macrophage cells with lipopolysaccharide. These data suggest that nutritional supplements such as Aquamin may play an important role in regulating the inflammatory response by modulating the nuclear factor kappa B signalling pathway. Copyright © 2011 John Wiley & Sons, Ltd.


Ryan S.,University College Cork | O'Gorman D.M.,Marigot Ltd | Nolan Y.M.,University College Cork
Phytotherapy Research | Year: 2011

It is well established that neuroinflammation contributes to brain aging, and that cortical cells are particularly vulnerable. Lipopolysaccharide stimulates the release of the pro-inflammatory cytokines, tumor necrosis factor-alpha and interleukin-1beta from glial cells which consequently induces an impairment in neuronal cell function. The food supplement, Aquamin, is a natural, multi-mineral derived from the red algae Lithothamnion corallioides, rich in calcium, magnesium and 72 other trace minerals. The aim of this study was to evaluate the anti-inflammatory potential of Aquamin in lipopolysaccharide-stimulated, glial-enriched primary cultures of rat cortex. It is reported that Aquamin prevented lipopolysaccharide-induced secretion of tumor necrosis factor-alpha and interleukin-1beta from cortical glia. These data suggest that nutritional supplements such as Aquamin may play an important role in impeding the detrimental effects of excessive inflammation in the brain. © 2010 John Wiley & Sons, Ltd.


O'Gorman D.M.,Marigot Ltd. | Tierney C.M.,Royal College of Surgeons in Ireland | Brennan O.,Royal College of Surgeons in Ireland | Brennan O.,Trinity College Dublin | And 2 more authors.
Phytotherapy Research | Year: 2012

Osteoporosis is a global health problem characterized by low bone mass and an increase in bone fragility. It is now well accepted that dietary factors play a central role in bone development and health. Diet that lacks adequate minerals is considered to be a risk factor for osteoporosis. The food supplement, Aquamin, is a natural, multi-mineral derived from the red algae Lithothamnion corallioides, rich in calcium, magnesium and 72 other trace minerals. The aim of this study was to evaluate the effect of Aquamin on osteoblastic behaviour and mineralisation in a pre-osteoblastic cell line. Cell number and metabolic activity were assessed using Hoescht DNA and Alamar Blue assays respectively. Osteogenic differentiation was measured using an alkaline phosphatase assay while mineralisation was determined using von Kossa and alizarin red staining. It is reported here that Aquamin promotes increased mineralisation in osteoblast cell culture. These data suggest that the nutritional supplement Aquamin plays an important role in promoting bone formation and may be useful in treating bone diseases such as osteoporosis. Copyright © 2011 John Wiley & Sons, Ltd.


PubMed | Marigot Ltd. and Royal College of Surgeons in Ireland
Type: | Journal: Journal of the mechanical behavior of biomedical materials | Year: 2015

Aquamin is a commercially-available supplement derived from the algae species Lithothamnion, which has proven osteogenic potential. By harnessing this potential and combining Aquamin with a collagen scaffold, with architecture and composition optimised for bone repair, the aim of this study was to develop a natural osteo-stimulative bone graft substitute. A fabrication process was developed to incorporate Aquamin into scaffolds to produce collagen-Aquamin (CollAqua) scaffolds at two different Aquamin concentrations, 100F or 500F (equivalent weight% of collagen or five times the weight of collagen respectively). CollAqua constructs had improved mechanical properties which were achieved without reducing the scaffolds permeability or porosity below the minimum level required for successful bone tissue engineering. The fabrication process produced a homogenous Aquamin distribution throughout the scaffold. Release kinetics revealed that in the first 12h, the entire Aquamin content was released from the 100F however, less than half of Aquamin in the 500F was released with the remainder released approximately 21 days later giving an initial burst release followed by a delayed release. Osteoblasts cultured on the CollAqua scaffolds showed improved osteogenesis as measured by alkaline phosphatase, osteopontin and osteocalcin expression. This was confirmed by increased mineralisation as determined by von Kossa and Alizarin red staining. In conclusion, a cell and growth factor free collagen-based bone graft substitute with enhanced mechanical properties has been developed. The addition of Aquamin to the collagen biomaterial significantly improved mineralisation by osteoblasts and results in a new product which may be capable of enhancing osteogenesis to facilitate bone repair in vivo.


Grant
Agency: European Commission | Branch: FP7 | Program: BSG-SME | Phase: SME-1 | Award Amount: 1.21M | Year: 2008

The overall aim of the project is to realize a commercial opportunity to produce low molecular weight polysaccharides (LMWP) from alginate- and agar-bearing seaweeds for applications in food & health, and wellness products by a group of SMEs. The aim will be addressed by an integrated workplan comprising 1 management activity 5 interlinked RTD workpackages, and a dissemination and exploitation activity. In the RTD programme, Cybercolloids (CC) will produce and characterize 8 novel, LMWPs from alginate and agar raw materials provided by 2 SMEs - ROKO (RO) and Hebridean Seaweed (HS). The LMWPs will be screened using batch culture fermentations with human faecal bacteria, for prebiotic activity (i.e. ability to modulate beneficially the gut microflora) by University of Reading (UREAD). The 2 most effective agar and alginate LMWPs will be selected and bulk produced by CC for validation of prebiotic activity in a feeding trial in human volunteers (University of Ulster)). The end points to be assessed will be stimulation of beneficial bacteria (bifidogenic effects), increased short chain fatty acid production, beneficial effects on stool formation, and improvements in gut barrier function (assessed by an in vitro method). Additionally, benefits of the LMWPs towards plasma lipid profiles and blood glucose levels will be assessed. Alongside the human study, a detailed investigation of the effects of the selected LMWPs in an in vitro model of the human colon will be conducted by UREAD to provide supporting evidence for beneficial effects on bacterial types and activities in the colon. RO and HS in conjunction with CC will implement LMWP technology transfer and scale-up to facilitate production at an industrial scale. Subsequently, another SME Marigot Ltd, will provide a route to market and in conjunction with RO, HS and CC will exploit, with respect to the health and wellness sector, the relevant knowledge provided on the efficacy of novel LMWPs from alginate and agar.


Grant
Agency: European Commission | Branch: FP7 | Program: BSG-SME | Phase: SME-1 | Award Amount: 1.33M | Year: 2010

The project is designed to address a commercial opportunity to obtain bioactive compounds from seaweeds for application in food and health & wellness products. Although polyphenols from land plants are widely used as functional food ingredients and food supplements, seaweed sources have been little studied or exploited. The consortium comprises 4 SME partners Hebridean Seaweed, Marigot, Coressence and Mesosystem, and 3 RTD providers CyberColloids and Universities of Reading and Ulster. In the first stage of the project seaweed polyphenol extracts (SPEs) will be prepared. One of the SPEs will be produced at food grade quality from Ascophyllum. In addition, a series of novel polyphenol extracts from a range of seaweed types of relevance to the partner SMEs, will be prepared. All the extracts will be characterised for their polyphenol content. The antioxidant and anti-inflammatory activities of the food grade SPE will be assessed in a dietary intervention trial in human subjects. The end points will include prevention of oxidative DNA damage in lymphocytes, and modulation of anti-inflammatory and pro-inflammatory cytokines in plasma. In addition a short-term dietary intervention in human volunteers will be conducted to assess bioavailability and metabolism of the component polyphenols in the extract. Finally the novel SPEs will be investigated for anti-inflammatory and anti oxidant activity in vitro. The food grade SPE will be included as a comparator. In this way it should be possible to extrapolate from the in vitro activities to provide an estimate of the likely in vivo effects in terms of anti-oxidant and anti-inflammatory properties and to provide the SMEs with candidate extracts with potential beneficial properties that can be further developed at a later stage. The SME partners in conjunction with Cybercolloids will implement polyphenol extract technology transfer and formulate a route to market


Trademark
MARIGOT Ltd | Date: 2016-02-09

Nutritional additives for medical purposes, namely, seaweed-based and algae-based additives for use in food and for use in vitamin and mineral supplements for human consumption and nutritional goods and beverages.


Bernard J.K.,University of Georgia | West J.W.,University of Georgia | Mullis N.,University of Georgia | Wu Z.,University of Georgia | And 2 more authors.
Professional Animal Scientist | Year: 2014

Thirty-six lactating Holstein cows were used in a randomized design trial to evaluate the effect of feeding supplemental calcareous marine algae on performance and select metabolic indices of health of Holstein cows in early lactation. Treatments included no supplement (NEG), 204 g/d of sodium bicarbonate (POS), or 87 g/d of calcareous marine algae (AB, Acid Buf, Celtic Sea Minerals). Beginning at 14 ± 4 DIM, all cows were fed NEG for 2 wk before being assigned randomly within parity and calving date to treatment for the following 10 wk. An interaction of treatment and week was observed for DMI, which was lowest during wk 1 and greatest during wk 9 and 10 for POS compared with NEG and AB. No differences were observed for yield of milk, components, or energy-corrected milk. Milk protein percentage tended to be greater for NEG compared with NEG and AB. An interaction of treatment and week was observed for efficiency of milk production (energy-corrected milk/ DMI) because efficiency was greatest for POS during wk 1 compared with all other treatments, but was greatest for AB during wk 8 to 10 compared with NEG and POS. Concentrations of MUN were greater for AB compared with NEG, but not different from POS. Serum glucose concentrations were greater for NEG compared with POS and AB. No differences were observed in concentrations of other serum metabolites or enzymes. Supplemental calcareous marine algae supported similar performance but improved efficiency of milk production during the wk 8 to 10 of the trial compared with diets with or without sodium bicarbonate. © 2014 American Registry of Professional Animal Scientists.


PubMed | Marigot Ltd
Type: Journal Article | Journal: Phytotherapy research : PTR | Year: 2012

Osteoporosis is a global health problem characterized by low bone mass and an increase in bone fragility. It is now well accepted that dietary factors play a central role in bone development and health. Diet that lacks adequate minerals is considered to be a risk factor for osteoporosis. The food supplement, Aquamin, is a natural, multi-mineral derived from the red algae Lithothamnion corallioides, rich in calcium, magnesium and 72 other trace minerals. The aim of this study was to evaluate the effect of Aquamin on osteoblastic behaviour and mineralisation in a pre-osteoblastic cell line. Cell number and metabolic activity were assessed using Hoescht DNA and AlamarBlue assays respectively. Osteogenic differentiation was measured using an alkaline phosphatase assay while mineralisation was determined using von Kossa and alizarin red staining. It is reported here that Aquamin promotes increased mineralisation in osteoblast cell culture. These data suggest that the nutritional supplement Aquamin plays an important role in promoting bone formation and may be useful in treating bone diseases such as osteoporosis.

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