Mariculture Research Center for Subtropical Shellfish and Algae of Guangdong Province

Shantou, China

Mariculture Research Center for Subtropical Shellfish and Algae of Guangdong Province

Shantou, China
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Zhang Q.,Shantou University | Zhang Q.,Mariculture Research Center for Subtropical Shellfish and Algae of Guangdong Province | Lu Y.,Shantou University | Lu Y.,Mariculture Research Center for Subtropical Shellfish and Algae of Guangdong Province | And 6 more authors.
Fish and Shellfish Immunology | Year: 2016

Vitellogenin (Vg), an egg yolk precursor protein, not only functions as a source of nutrients and a nonpolar molecular carrier that combine and transfer lipids, proteins, vitamin and carotenoids to oocytes during the oogenesis. but also links with the immune defense in many oviparous animals. To investigate whether Vg plays a immune defensive role in noble scallop Chlamys nobilis, an acute Vibrio anguillarum infection experiment was conducted in orange and brown scallops with different carotenoids content. qRT-PCR result showed that Vg transcripts were significantly up-regulated after challenge with V. anguillarum in orange and brown shell scallops compared to the control group and Vg expression reached the highest spot at 6 h, indicated that Vg possessed an immune function in the noble scallop. Interestingly, a significantly positive correlation between Vg transcript levels and total carotenoids content in the ovary was observed, indicating that Vg gene expression was up regulated by carotenoids. The results suggest that Vg is a potent immune protector and carotenoid may linked with Vg plays an important role in host immune system against pathogens in noble scallop C. nobilis. © 2016 Elsevier Ltd.


Han J.,Shantou University | Han J.,Mariculture Research Center for Subtropical Shellfish and Algae of Guangdong Province | Lu Y.,Shantou University | Lu Y.,Mariculture Research Center for Subtropical Shellfish and Algae of Guangdong Province | And 8 more authors.
Fish and Shellfish Immunology | Year: 2016

The noble scallop Chlamys nobilis belongs to a warm-water mollusk and has been cultured in the sea of southern China since 1980s'. However, accidents of massive mortality have often occurred during the winter, and one of the reasons could be accumulation of harmful reactive oxygen species caused by lower temperature. Carotenoids are well known for their anti-oxidant function. To investigate whether carotenoids do play a role in mollusks' antioxidant defense system under lower temperature stress, an acute lower temperature experiment was conducted by using two types of scallops: the orange with higher carotenoids content and the brown with lower carotenoids content. Their CuZnSOD gene was cloned, mRNA expression levels were determined, and SOD activity and carotenoids content were measured. The complete CuZnSOD cDNA consists of 1078 nucleotides with an open reading frame encoding 154 amino acid residues, which has high identity with that of its sister species Chlamys farreri. The mRNA expression levels in both the mantle and gill from the orange scallops were significantly higher (P < 0.05) than that of the brown ones, but the result was the opposite in the blood. SOD activity in the mantle and gill from the orange scallops was significantly higher than (P < 0.05) that from the brown ones. Further, significantly positive correlations were found among CuZnSOD gene transcript levels, SOD activity and total carotenoids content in the orange scallops. The present results suggested that carotenoids could play roles in antioxidant defense system by upregulating gene expression under lower temperature stress in the noble scallop. © 2016 Elsevier Ltd.

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