Mariculture Institute of Shandong Province
Mariculture Institute of Shandong Province
Diao J.,Mariculture Institute of Shandong Province |
Ye H.-B.,Mariculture Institute of Shandong Province |
Yu X.-Q.,Mariculture Institute of Shandong Province |
Fan Y.,Mariculture Institute of Shandong Province |
And 3 more authors.
Veterinary Immunology and Immunopathology | Year: 2013
In order to investigate the adjuvant and immunostimulatory effects of LPS and β-glucan, a highly effective vaccine candidate for Edwardsiella tarda known as glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was recombinantly expressed in Escherichia coli, which was intramuscularly injected into Japanese flounder alone or containing LPS or β-glucan. ELISA analysis of sera showed that the fish immunized with rGAPDH containing LPS or β-glucan had significant higher antibody titers than rGAPDH immunization group at day 14 and 30 ( P<. 0.05). Flow cytometric analysis showed that the surface immunoglobulin positive (sIg+) lymphocytes percentages in head kidney of the immunized fish exhibited significant higher than the fish of control group at day 14 and 30, and immunization with rGAPDH containing LPS or β-glucan could induce significant higher sIg+ percentages than immunization with rGAPDH alone. Simultaneously, the expressions of genes coding for immunoregulatory molecules involved in innate and acquired responses, including IFN-γ, IL-1β, IL-6, TNF-α, TCRα, IgM, C-type lysozyme and MHC-IIα were analyzed by quantitative PCR. The transcription levels of all selected genes except TNF-α displayed up-regulation after immunization, and immunization with rGAPDH containing LPS or β-glucan could induce significant higher levels than that induced only by rGAPDH. Moreover, to investigate the protection against infection, challenge with E. tarda was performed at day 30 after immunization. Fish immunized with rGAPDH containing β-glucan exhibited the highest relative percentage survival (RPS) of 63% compared to the other groups. These results indicated that LPS and β-glucan employed as co-immunostimulatory molecules could significantly trigger the innate and acquired immune responses in Japanese flounder. © 2013 Elsevier B.V.
Sun M.,Chinese Academy of Fishery Sciences |
Sun M.,Ocean University of China |
Li J.,Chinese Academy of Fishery Sciences |
Gai C.L.,Chinese Academy of Fishery Sciences |
And 4 more authors.
Journal of Veterinary Pharmacology and Therapeutics | Year: 2014
In this study, the pharmacokinetics profiles of difloxacin in the olive flounder (Paralichthys olivaceus) were investigated following intravenous and oral administration (10 mg/kg BW) at 14 and 22 °C water temperatures. Plasma and tissue samples (muscle, liver, and kidney) were analyzed using an HPLC method. The results showed that the plasma concentration-time data for difloxacin were described commendably by two-compartment open model at the two water temperatures. The absorption half-life (t1/2ka) of difloxacin after oral administration were 2.08 and 1.10 h at 14 and 22 °C, respectively; whereas the elimination half-life (t1/2β) was 4.41 and 2.38 h, respectively. The muscle concentration of 1.35 ± 0.19 μg/g was observed at 9 h at 14 °C, and 2.11 ± 0.33 μg/g at 6 h at 22 °C, respectively. For liver, the peak concentration of difloxacin 2.43 ± 0.30 μg/g occurred at 6 h at 14 °C, which was lower than the 3.34 ± 0.24 μg/g peak that occurred at 4 h at 22 °C. The calculated bioavailability of difloxacin was 68.07% at 22 °C, which was higher than the 53.43% calculated for 14 °C. After intravenous administration, the t1/2β were 4.79 and 2.81 h at 14 and 22 °C, respectively. The results indicate that the peak concentrations in muscle and liver at 14 °C are approximately half of those achieved at 22 °C. However, the Cmax in kidney at 14 and 22 °C were similar. The Vd values were 1.20 and 1.75 L/kg at 14 and 22 °C, respectively. These data indicated that both temperature and drug administration had significant effects on the elimination of difloxacin, and lower temperature or oral administration resulted in lower elimination. © 2013 John Wiley & Sons Ltd.
Shuxian W.,Mariculture Institute of Shandong Province |
Jianteng W.,CAS Qingdao Institute of Oceanology |
Tianbao L.,Mariculture Institute of Shandong Province
Journal of Basic Microbiology | Year: 2012
Vibrio alginolyticus, one of the most important opportunistic pathogens, can be detected in human being and marine animals. Like other bacteria, V. alginolyticus is able to adapt to a variety of stressful environmental changes. The alternate sigma factor RpoS, which is a regulator during stationary phase, plays an important role in surviving under these stressful situations in many bacteria. Sequence analysis reveals a 990 bp open reading frame which is predicted to encode a 330-amino-acid protein with 68% to 96% overall identity to other reported sequences. To study the function of rpoS, the rpoS gene of V. alginolyticus VIB283 was cloned and an rpoS mutant was constructed by homologous recombination. Comparison of the study result of the wild type and the mutant showed that the mutant was more sensitive to stress conditions such as high osmolarity, oxidative stress, heat shock, and long-term starvation and that the LD50 of the mutant strain to the zebra fish was about 2.8 times as that of the control strain. In addition, the SDS-PAGE analysis indicated that the outer membrane proteins (OMPs) existed great differences. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Wei J.,CAS Lanzhou Institute of Chemical Physics |
Wang S.,Mariculture Institute of Shandong Province |
Liu G.,CAS Qingdao Institute of Oceanology |
Pei D.,CAS Lanzhou Institute of Chemical Physics |
And 2 more authors.
International Journal of Biological Macromolecules | Year: 2014
The effects of polysaccharides from Enteromorpha prolifera (PEP) on cell-mediated immunity, humoral immunity and mononuclear phagocytic system function were evaluated to assess the immunomodulatory potential of these macromolecules. Relevant immunological mechanisms were verified by biochemical assays and western blot analysis. Results showed that PEP could induce splenocyte proliferation. In vivo experiments on Kunming mice confirmed that PEP could improve cell-mediated immunity, humoral immunity and mononuclear phagocytic system function. To illustrate the mechanism, we determined several immune-related enzymes in the thymus and spleen. The results indicated that PEP could enhance the activities of alkaline phosphatase, superoxide dismutase and lactate dehydrogenase. PEP could also increase the level of NF-κB. These results suggested that PEP exhibited potent immunomodulatory properties and could be used as a novel potential immunostimulant in food and pharmaceutical industries. © 2013 Elsevier B.V.
Wang X.,CAS Qingdao Institute of Oceanology |
Wang X.,Tianjin Fisheries Research Institute |
Yang H.,CAS Qingdao Institute of Oceanology |
Liu G.,Mariculture Institute of Shandong Province |
Wang Q.,CAS Qingdao Institute of Oceanology
Chinese Journal of Oceanology and Limnology | Year: 2011
To assess the toxicity of heavy metal pollution to marine intertidal shellfish, enzymatic responses and lipid peroxidation were investigated in the clam Mactra vereformis exposed to cadmium under laboratory conditions. Three antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase, GPx), two immune defense enzymes (acid phosphatase, ACP; alkaline phosphatase, ALP), and one lipid peroxidation product (malondialdehyde, MDA) were measured in the gills and the hepatopancreas of the clam exposed to 0, 25, 75, and 125 μg/L cadmium for 0, 1, 3, 5, and 7 d. The results show that the concentrations of antioxidant enzymes in the organs soared to a peak value on the first day and then decreased afterwards in most cases. CAT and GPx activities in the hepatopancreas were higher than in the gills, but the SOD activity was lower in the hepatopancreas. ACP activity was unchanged until Day 3 in the hepatopancreas and until Day 5 in gills, when it began to increase. ALP activity showed no significant relationship with Cd treatment. MDA concentrations increased in the two tissues after Cd exposure, peaked on Day 3 in gills, and on Day 5 in hepatopancreas. These observations show that changes in the activities of antioxidant enzymes and ACP reflect the time course of oxidative stress in the clam caused by Cd, and could be used as potential biomarkers for ecotoxicological bioassays of heavy metals. © 2011 Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg.
Xu Z.,Xiamen University |
Xu Z.,Mariculture Institute of Shandong Province |
Gao K.,Xiamen University
Marine Pollution Bulletin | Year: 2012
Solar ultraviolet radiation (UVR, 280-400. nm) is known to inhibit the photosynthesis of macroalgae, whereas nitrogen availability may alter the sensitivity of the algae to UVR. Here, we show that UV-B (280-315. nm) significantly reduced the net photosynthetic rate of Gracilaria lemaneiformis. This inhibition was alleviated by enrichment with ammonia, which also caused a decrease in dark respiration. The presence of both UV-A (315-400. nm) and UV-B stimulated the accumulation of UV-absorbing compounds. However, this stimulation was not affected by enrichment with ammonia. The content of phycoerythrin (PE) was increased by the enrichment of ammonia only in the absence of UVR. Ammonia uptake and the activity of nitrate reductase were repressed by UVR. However, exposure to UVR had an insignificant effect on the rate of nitrate uptake. In conclusion, increased PE content associated with ammonia enrichment played a protective role against UVR in this alga, and UVR differentially affected the uptake of nitrate and ammonia. © 2011 Elsevier Ltd.
PubMed | CAS Lanzhou Institute of Chemical Physics, Mariculture Institute of Shandong Province and CAS Qingdao Institute of Oceanology
Type: | Journal: International journal of biological macromolecules | Year: 2014
The effects of polysaccharides from Enteromorpha prolifera (PEP) on cell-mediated immunity, humoral immunity and mononuclear phagocytic system function were evaluated to assess the immunomodulatory potential of these macromolecules. Relevant immunological mechanisms were verified by biochemical assays and western blot analysis. Results showed that PEP could induce splenocyte proliferation. In vivo experiments on Kunming mice confirmed that PEP could improve cell-mediated immunity, humoral immunity and mononuclear phagocytic system function. To illustrate the mechanism, we determined several immune-related enzymes in the thymus and spleen. The results indicated that PEP could enhance the activities of alkaline phosphatase, superoxide dismutase and lactate dehydrogenase. PEP could also increase the level of NF-B. These results suggested that PEP exhibited potent immunomodulatory properties and could be used as a novel potential immunostimulant in food and pharmaceutical industries.
Liu H.,Mariculture Institute of Shandong Province |
Liu M.,Mariculture Institute of Shandong Province |
Ge S.,Ocean University of China |
Wang Q.,Mariculture Institute of Shandong Province |
And 2 more authors.
Biochemical Systematics and Ecology | Year: 2012
To investigate the genetic diversity, population structure and historical demography of Perinereris aibuhitensis, a 684 base pair fragment of the mitochondrial DNA cytochrome oxidase subunit I gene was analyzed in 92 organisms sampled from around the coasts of Shandong Peninsula. The results showed low haplotype diversity and nucleotide diversity in P. aibuhitensis. Topologies of neighbor-joining trees were shallow but the frequencies of common haplotypes indicated recent evolutionary lineages in the associated geographic areas. Significant genetic differentiations were detected among the four sampled populations, reflecting limited gene flow. Neutrality tests and mismatch distributions showed population expansion in P. aibuhitensis, but a recent bottleneck or selection event is indicated for the samples in the Yellow River Estuary and the Jiaozhou Bay. © 2012 Elsevier Ltd.
Tang X.,Ocean University of China |
Diao J.,Mariculture Institute of Shandong Province |
Zhan W.,Ocean University of China |
Sheng X.,Ocean University of China
Aquaculture | Year: 2012
Flounder gill (FG) cell line derived from the gill tissue of Japanese flounder (Paralichthys olivaceus) was employed as a target cell for investigating the molecules involved in lymphocystis disease virus (LCDV) binding and infection. Indirect immunofluorescence assay showed that LCDV could attach to the FG cells membrane susceptibly. By virus overlay protein binding assay (VOPBA), a 37.6. kDa LCDV-binding protein was detected in FG cells' membrane. Two dimensional gel electrophoresis analysis revealed that the 37.6. kDa protein was a single polypeptide with PI of 6.0. Mass spectrometric analysis showed that the 37.6. kDa protein had a strong association with a coiled-coil domain-containing protein. While treated with trypsin or sodium periodate, the binding ability of the 37.6. kDa protein to LCDV was inhibited, which suggested that sugar chains of the 37.6. kDa glycosylated protein were involved in virus binding. Polyclonal antibodies were obtained by immunizing the rabbit with electroeluted 37.6. kDa protein, which showed a dose-dependent blocking effect to the binding between LCDV and 37.6. kDa protein in modified VOPBA, and also inhibited LCDV infection to FG cells in virus infection assay. These results demonstrated that the 37.6. kDa trypsin-sensitive glycoprotein might be a potential receptor for LCDV infection to FG cells. © 2012 Elsevier B.V.
Du R.,Yantai University |
Liu L.,Yantai University |
Liu L.,Ocean University of China |
Wang A.,Yantai University |
Wang Y.,Mariculture Institute of Shandong Province
Chinese Journal of Oceanology and Limnology | Year: 2013
Gracilaria asiatica, being highly efficient in nutrient absorption, is cultivated in sea cucumber ponds to remove nutrients such as nitrogen and phosphate. It was cultured in a laboratory simulating field conditions, and its nutrient absorption was measured to evaluate effects of environmental conditions. Ammonia nitrogen (AN), nitrate nitrogen (NN), total inorganic nitrogen (TIN), and soluble reactive phosphorus (SRP) uptake rate and removal efficiency were determined in a 4×2 factorial design experiment in water temperatures (T) at 15°C and 25°C, algae biomass (AB) at 0. 5 g/L and 1. 0 g/L, total inorganic nitrogen (TIN) at 30 μmol/L and 60 μmol/L, and soluble reactive phosphorus (SRP) at 3 and 6 μmol/L. AB and ambient TIN or SRP levels significantly affected uptake rate and removal efficiency of AN, NN, TIN, and SRP (P< 0. 001). G. asiatica in AB of 0. 5 g/L showed higher uptake rate and lower removal efficiency relative to that with AB of 1. 0 g/L. Nitrogen and phosphorus uptake rate rose with increasing ambient nutrient concentrations; nutrient removal efficiency decreased at higher environmental nutrient concentrations. The algae preferred to absorb AN to NN. Uptake rates of AN, NN, and SRP were significantly affected by temperature (P < 0. 001); uptake rate was higher for the 25°C group than for the 15°C group at the initial experiment stage. Only the removal efficiency of AN and SRP showed a significant difference between the two temperature groups (P< 0. 01). The four factors had significant interactive effects on absorption of N and P, implying that G. asiatica has great bioremedial potential in sea cucumber culture ponds. © 2013 Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg.