Maria Sklodowska Curie Memorial Cancer Center and Institute Warsaw Poland

Warsaw Poland, Poland

Maria Sklodowska Curie Memorial Cancer Center and Institute Warsaw Poland

Warsaw Poland, Poland
SEARCH FILTERS
Time filter
Source Type

Nasilowska-Adamska B.,Institute of Hematology and Transfusion Medicine Warsaw Poland | Warzocha K.,Institute of Hematology and Transfusion Medicine Warsaw Poland | Solarska I.,Institute of Hematology and Transfusion Medicine Warsaw Poland | Borg K.,Institute of Hematology and Transfusion Medicine Warsaw Poland | And 2 more authors.
European Journal of Haematology | Year: 2017

Purpose: FMS-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD) is aberration associated with poor prognosis in AML. We have analyzed the expression of MDR-1, MRP-1, and BCRP mRNA in relation to FLT3-ITD in 100 AML adult patients with normal and intermediate karyotype. Methods: The RQ-PCR method was performed to assess the expression of MDR-1, MRP-1, and BCRP mRNA, and the results were presented as coefficients calculated using an intermediate method according to Pfaffl's rule. Results: According to univariate analysis, the following pretreatment variables negatively influenced disease-free survival (DFS): WBC count ≥25×109/L (P=.037), MRP-1 mRNA ≥1.6818 (P=.028), BCRP mRNA ≥1.1892 (P=.004), FLT3-ITD (P=.005) and overall survival (OS): WBC count ≥25×109/L (P=.031), MRP-1 mRNA ≥1.6818 (P=.01), BCRP mRNA ≥1.1892 (P=.01), FLT3-ITD (P=.001). When all prognostic variables were pooled into a multivariate model, we found that WBC count ≥25×109/L (P=.026) and BCRP mRNA ≥1.1892 (P=.011). We observed trend in negative influence of FLT3-ITD on DFS (P=.057). BCRP mRNA ≥1.1892 (P=.035) and FLT3-ITD (P=.006) negatively, independently influenced the OS. Conclusions: The high expressions of BCRP mRNA calculated with Pfaffl's rule and FLT3-ITD are independent poor risk factors in adult patients with AML and intermediate or normal karyotype. © 2017 John Wiley & Sons A/S.

Loading Maria Sklodowska Curie Memorial Cancer Center and Institute Warsaw Poland collaborators
Loading Maria Sklodowska Curie Memorial Cancer Center and Institute Warsaw Poland collaborators