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Diyarbakr, Turkey

Kendal E.,Mardin Artuklu University
Journal of Agricultural Science and Technology | Year: 2015

The aim of this study was to evaluate Genotype×Environment Interaction (GEI) of chlorophyll meter readings (SPAD) of ten durum wheat cultivars, using data obtained from multi-environment trials during two years, at eight locations. Stability and genotypic superiority for SPAD reading was identified using ANOVA and GGE biplot analysis. Furthermore, the interrelationships among SPAD reading and other yield components and cultivars-by-traits, environment-by-traits, and cultivars-by-environment were studied using scatter, ranking, and comparison of biplot techniques. Substantial variations were found among SPAD reading, yield components, and quality criteria as related to each other, environment, and cultivars. There were positive correlations among SPAD reading with GY, some quality criteria [Protein Content (PC); Wet Gluten (WG), Vitreous Kernels (VIT)], and yield components [stalks m-2 (SS); ear m-2 (ES); Plant Height (PH), Length of Spike (LS)], while Maturation Time (MT) had negative correlation with SPAD. Also, there was relationship among SPAD and SC (Semolina Color) and SDS (Mini Sedimentation) with obtuse angles (< 900), but the correlation was not significant. The GGE biplot indicated that Kızıltepe environment (E6) and Eyyubi cultivar (G3) were the best in terms of SPAD reading. The GGE biplot provided useful information for experimentation of SPAD readings of cultivars when grown under multienvironment. Moreover, SPAD should be considered as the preferred tool, when the breeder is looking for the best and useful tool to determine flag leaf chlorophyll content. © 2015, Tarbiat Modares University. All rights reserved. Source


Kilinc E.,Mardin Artuklu University
Artificial Cells, Nanomedicine and Biotechnology | Year: 2016

Hybrid magnetic nanoparticles composed from C60 fullerene and γ-Fe2O3 were synthesized by hydrothermal method. XRD, FT-IR, VSM, SEM, and HR-TEM were employed for characterizations. The magnetic saturation value of C60-γ-Fe2O3 magnetic nanoparticles was 66.5 emu g-1. Concentration of Fe in nanoparticles asdetermined by ICP-OES was 40.7% Fe. Particle size of C60-γ-Fe2O3 magnetic nanoparticles was smaller than 10 nm. Maximum adsorption capacity of C60-γ-Fe2O3 for flurbiprofen, a non-steroidal anti-inflammatory drug, was calculated from Langmuir isotherm as 142.9 mg g-1. © Copyright 2014 Informa Healthcare USA, Inc. Source


In this research, a procedure for the preconcentration of Bi at trace levels in milk samples prior to inductively coupled plasma optical emission spectrometry (ICP-OES) is proposed. It is based on the batch magnetic solid phase extraction of Bi ions by fullerene C60 modified maghemite (γ-Fe2O3 superparamagnetic iron oxide nanoparticles-SPION) nanoparticles. The various factors that influence the preconcentration of Bi such as pH, the amount of magnetic nanoparticles, initial Bi concentration, sample volume and the effects of possible interfering ions were investigated. A linear analytical curve was achieved in the concentration range of 0.25-10 ng mL-1 with a limit of detection of 0.06 ng mL-1. The precision expressed as a RSD was 7.5% for a concentration of 0.25 ng mL-1. The method was validated by the analysis of a standard reference material (NIST SRM 1643e). In addition, 96.6-98.7% recoveries were achieved for the spiked samples. Bi concentrations in ten milk samples were successfully determined after the developed method was applied. Bi content in the milk samples ranged from 6.5 to 14.3 ng mL-1. © 2015 The Royal Society of Chemistry. Source


Keskin C.,Mardin Artuklu University
Asian Pacific Journal of Cancer Prevention | Year: 2015

Background: Cancer is an unnatural type of tissue growth in which the cells exhibit unrestrained division, leading to a progressive increase in the number of dividing cells. It is now the second largest cause of death in the world. The present study concerned antioxidant, anticancer and anticholinesterase activities and protocatechuic, catechin, caffeic acid, syringic acid, p-coumaric acid and o-coumaric concentrations in methanol extracts of flowers, fruits and seeds of Hypericum amblysepalum. Materials and Methods: Antioxidant properties including free radical scavenging activity and reducing power, and amounts of total phenolic compounds were evaluated using different tests. Protocatechuic, catechin, caffeic acid, syringic acid, p-coumaric acid and o-coumaric concentrations in extracts were determined by HPLC. Cytotoxic effects were determined using the MTT test with human cervix cancer (HeLa) and rat kidney epithelium cell (NRK-52E) lines. Acetyl and butyrylcholinesterase inhibitory activities were measured by by Ellman method. Results: Total phenolic content of H. amblysepalum seeds was found to be higher than in fruit and flower extracts. DPPH free radical scavenging activity of the obtained extracts gave satisfactory results versus butylated hydroxyanisole and butylated hydroxytoluene as controls. Reducing power activity was linearly proportional to the studied concentration range: 10-500 μg/mL LC50 values for H. amblysepalum seeds were 11.7 and 2.86 respectively for HeLa and NRK-52E cell lines. Butyryl-cholinesterase inhibitory activity was 76.9 ± 0.41 for seed extract and higher than with other extracts. Conclusions: The present results suggested that H. amblysepalum could be a potential candidate anti-cancer drug for the treatment of human cervical cancer, and good source of natural antioxidants. Source


In recent years, magnetic nanoparticles attained special interest in nanobiotechnology and nanomedicine due to their uniqe properties and biocompatibilities. From this perspective, hybride nanostructure composed from γ-Fe2O3 magnetic nanoparticle and carboxylated multi walled carbon nanotube was synthesized and characterized by FT-IR, VSM, SEM, HR-TEM and ICP-OES. Microscopy images showed that magnetic nanoparticles were nearly spherical structure that arranged on the axis of carboxylated MWCNT. Particle size was found lower than 10 nm. VSM results showed that the obtained magnetic nanoparticles presented superparamagnetic properties at room temperature. The magnetic saturation value was determined as 35.2 emu/g. It was used for the adsorption and controlled release of harmane, a potent tremor-producing neurotoxin. Maximum adsorption capacity was calculated as 151.5 mg/g from Langmuir isotherm. Concentration of harmane was determined by HPLC with fluorescence detection. The antimicrobial activity of synthesized magnetic nanoparticle was investigated against gram-negative and gram-positive bacteria. However, no activity was observed. © 2015 Elsevier B.V. All rights reserved. Source

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