Maratha Mandals Ngh Institute Of Dental Science And Research Center

Belgaum, India

Maratha Mandals Ngh Institute Of Dental Science And Research Center

Belgaum, India
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Nayak A.,Maratha Mandals Ngh Institute Of Dental Science And Research Center | Nayak R.,Maratha Mandals Ngh Institute Of Dental Science And Research Center | Bankur P.K.,Guru Gobind Singh Dental College
Journal of Clinical and Diagnostic Research | Year: 2015

Background: The dimensions of gingiva and different parts of the masticatory mucosa have become a subject of considerable interest in Periodontics. Studies assessing the thickness of the facial gingiva are often seen in the literature. The thickness of the palatal gingiva is a subject still less researched in periodontal therapy and implantology. Objectives: To measure the thickness of the palatal gingiva using an ultrasound device ‘Biometric A- Scan’ and to evaluate the variation in the thickness of the palatal gingiva at the sites examined. Materials and Methods: In the 50 subjects examined, the thickness of the palatal gingiva was assessed at the maxillary anteriors, premolars and molars by an ultrasound device ‘Biometric A-Scan’. The results were subjected to statistical analysis using one-way ANOVA test and Newman-Keuls multiple post hoc procedure. Results: Statistically significant variations existed in the palatal gingival thickness. The thickness was highest at the lateral incisor region, followed by canine, premolars, molars and central incisor. Interpretation and Conclusion: In the subjects assessed, the thickness of the palatal gingiva at the lateral–canine area was the highest followed by the premolar area. In periodontal root coverage procedures and during implant therapy, we suggest the inclusion of the lateral incisor area, apart from the canine and premolar area, as a potential donor site for harvesting soft tissue grafts from the palatal area. However, the effect of several factors like age and sex of the patient, the anatomy of the palatal area, the influence of rugae patterns and racial and geographical differences should be taken into consideration prior to harvesting a graft from these sites. Apart from this, the study suggests that, the ultrasonographic measurements provide an elegant means of obtaining the measurements of gingival and mucosal tissues rapidly, accurately and non-invasively. Our endeavour in this research project attempts to open more avenues for studies in the field of advanced periodontal diagnosis, with the use of ultrasound, and expand the horizons of periodontal plastic surgery and implant therapy as well. © 2015, Journal of Clinical and Diagnostic Research. All rights reserved.


Ajeesh Kumar A.K.,Anthem Biosciences Pvt. Ltd. and 49 | Nair K.B.,Anthem Biosciences Pvt. Ltd. and 49 | Bodke Y.D.,Kuvempu University | Sambasivam G.,Anthem Biosciences Pvt. Ltd. and 49 | Bhat K.G.,Maratha Mandals Ngh Institute Of Dental Science And Research Center
Monatshefte fur Chemie | Year: 2016

Abstract: A series of novel carboxamides, sulfonamides, ureas, and thioureas derived from 1,2,4-oxadiazol-3-ylmethyl-piperazin-1-yl substituted with pyrazolo[1,5-a]pyrimidine analog were designed and synthesized. The newly synthesized compounds were characterized by 1H NMR, 13C NMR, ESI–MS, and IR and were tested for their in vitro antiproliferative activity by MTT assay. Out of these twenty derivatives, five compounds showed good anticancer activity against HeLa cell line. These are superior with less than 10 µg/cm3 of IC50 when compared to the marketed anticancer drug paclitaxel with 30 µg/cm3 of IC50 against Hela cell line. Graphical abstract: [Figure not available: see fulltext.] © 2016 Springer-Verlag Wien


Krishnamurthy S.,Maratha Mandals Ngh Institute Of Dental Science And Research Center | Hallikerimath R.B.,Maratha Mandals Ngh Institute Of Dental Science And Research Center
Journal of Clinical and Diagnostic Research | Year: 2016

Introduction: Colonization of the surface by Candida albicans and related Candida species is one of the major concerns of denture lining materials. Aim: We evaluated retention, colonization and penetration of the four denture lining materials namely Molloplast B, Permaflex, GC Soft Liner and Ufi Gel Hard C by Candida albicans. Material and Methods: a) Evaluation of retention: Five test discs of each material with smooth surface on one side and rough on the other were prepared and surface roughness (Ra) was measured with profilometer. Retention of C. albicans to discs was monitored after one hour of incubation (37ºC) with standardized (2.8 x 106 cfu/ml) washed cell suspension. Discs were stained with acridine orange and attached cells were counted using inverted microscope; b) Evaluation of colonization and penetration: Eight test discs of each material in sterile artificial saliva, were inoculated with C. albicans and incubated for six weeks. Two sections were cut across each test disc to provide three replicate samples. Candida cells on cut disc sections were fixed, dehydrated, air dried and viewed via fluorescence microscope; c) Evaluation of antifungal action: Two test discs of each material were placed onto diagnostic sensitivity testing the agar plate. After incubation at 37ºC for 24 hours, the zone of inhibition formed around the samples were measured at four places, and the mean calculated. Results: a) All rough surfaces showed higher retention of C. albicans than smooth surfaces. Among the smooth surfaces, Molloplast B and GC Soft Liner showed highest and lowest retention of C. albicans respectively (p=0.0090). Among the rough surfaces, the variation in the retention of C. albicans was not statistically significant; b) Penetration of C. albicans was observed through all three sections of the test discs of each material. There was no statistically significant difference among the test materials; c) Molloplast B and Permaflex produced a mean zone of inhibition of 16.9±4.8mm and 14.80±3.8mm respectively. Conclusion: a) Smoother surfaces retain fewer cells than rough surfaces; b) Denture lining materials permit infiltration of Candida through their structure; c) Denture lining materials have insignificant anti-fungal properties. © 2016, Journal of Clinical and Diagnostic Research. All rights reserved.


D'souza R.S.,Maratha Mandals Ngh Institute Of Dental Science And Research Center | Bhat K.G.,Maratha Mandals Ngh Institute Of Dental Science And Research Center | Sailaja D.,Gokaraju Rangaraju Institute of Engineering and Technology | Babji D.V.,Maratha Mandals Ngh Institute Of Dental Science And Research Center | And 2 more authors.
Journal of Clinical and Diagnostic Research | Year: 2013

Introduction: Toll-like receptors (TLRs) are an important component of immune system. Among them, TLR-2 plays a dominant role in the oral tissues in initiating inflammation in chronic periodontitis. Not many studies have been done on quantitative expression of TLR-2 by using immunofluorescent techniques (IFT) in oral tissues. In this study, the expression and localisation of TLR-2 were detected in gingival tissues of chronic periodontitis patients and healthy individuals. Material and Methods: Immuno Fluorescent Technique (IFT) was used for the expression and localization of TLR-2 in gingival tissue samples from 25 chronic periodontitis patients and from 25 healthy controls. Haematoxylin and Eosin staining was also done for all the samples to determine the histological characteristics of the gingival tissue samples. Result: Both healthy and periodontitis gingival tissues expressed TLR-2. We found that the expression level of TLR-2 was higher in all the periodontitis patients than in healthy individuals. We also found out that the expression of TLR-2 was higher in the epithelial cells than in the connective tissue cells. Conclusion: These data suggest a definite involvement of TLR-2 in initiating an inflammatory response in periodontal tissues. More studies are required to define the mechanisms and expression levels of TLR-2 in oral health and diseases.


Tantradi P.,Maratha Mandals Ngh Institute Of Dental Science And Research Center | Sreenivasan V.,Subharati Dental College | Kadaganche H.,Vaidik Dental College
Indian Journal of Dental Research | Year: 2010

Context: Caries epidemiological studies based on clinical examination alone tend to underestimate proximal caries. Aims: This study aims to determine the additional value of radiographic examination (bitewings) compared to clinical examination in assessing posterior proximal caries. It also aims to determine the radiographic multiplication /adjusting / correction factors by which a clinical decayed surface (D-S) and decayed-missing-filled surfaces (DMFS) score could be multiplied, to obtain an actual D-S and DMFS score Materials and Methods: Two groups of 100 subjects each, in the age range of 16-20 years, were examined clinically for caries and their DMFS index recorded. Both groups were subjected to bitewing radiographs to detect the clinically undiagnosed posterior proximal caries. The radiographic adjusting factors were determined for group A. To check its repeatability and thus its usefulness, radiographic correction factor was determined for Group B. Statistical Analysis: The results were statistically analyzed with the use of means, standard deviations, regression equation and "Z" test. Level of significance was determined at P > 0.05. Results: A significant number of clinically undetected proximal caries was diagnosed with the help of bitewing radiographs. The difference between the radiographic adjusting/correction factor for D-S and DMFS score for the two groups was statistically not significant (P > 0.05) thus verifying the repeatability of the radiographic correction factors. Conclusion: The radiographic correction factors can be used to obtain a more precise estimate of true caries prevalence in a particular study population without having to subject all individuals to radiographic examination.


PubMed | Maratha Mandals Ngh Institute Of Dental Science And Research Center and Gokaraju Rangaraju Institute of Engineering and Technology
Type: Journal Article | Journal: Indian journal of dental research : official publication of Indian Society for Dental Research | Year: 2016

The aim of this study was to analyze the expression level and localization of Toll-like receptor (TLR) 4 in gingival samples of healthy and chronic periodontitis subjects by indirect immunofluorescence technique (IFT).In this study, gingival tissue samples were obtained from 25 healthy and 25 periodontitis individuals. The tissues were processed and the initial characterization was done by hematoxylin and eosin staining. The expression and localization of the TLR4 receptor were determined in the epithelial and connective layer cells of the gingival tissue using the indirect IFT. Immunofluorescence images were acquired and quantitative expression of TLRs was analyzed by calculating the percentage of cells showing positive results.We found that the healthy control group exhibited significantly lower values of TLR4 expression in comparison with the periodontitis patients. We also found that in patients with periodontitis the concentration of TLR4 was higher in the epithelium as compared to their expression in connective tissue cells.These data suggested a definite involvement of TLR4 in initiating and progression of an inflammatory response in periodontitis.


PubMed | Maratha Mandals Ngh Institute Of Dental Science And Research Center
Type: Journal Article | Journal: Indian journal of dental research : official publication of Indian Society for Dental Research | Year: 2010

Caries epidemiological studies based on clinical examination alone tend to underestimate proximal caries.This study aims to determine the additional value of radiographic examination (bitewings) compared to clinical examination in assessing posterior proximal caries. It also aims to determine the radiographic multiplication/adjusting/correction factors by which a clinical decayed surface (D-S) and decayed-missing-filled surfaces (DMFS) score could be multiplied, to obtain an actual D-S and DMFS score.Two groups of 100 subjects each, in the age range of 16-20 years, were examined clinically for caries and their DMFS index recorded. Both groups were subjected to bitewing radiographs to detect the clinically undiagnosed posterior proximal caries. The radiographic adjusting factors were determined for group A. To check its repeatability and thus its usefulness, radiographic correction factor was determined for Group B.The results were statistically analyzed with the use of means, standard deviations, regression equation and Z test. Level of significance was determined at P > 0.05.A significant number of clinically undetected proximal caries was diagnosed with the help of bitewing radiographs. The difference between the radiographic adjusting/correction factor for D-S and DMFS score for the two groups was statistically not significant (P > 0.05) thus verifying the repeatability of the radiographic correction factors.The radiographic correction factors can be used to obtain a more precise estimate of true caries prevalence in a particular study population without having to subject all individuals to radiographic examination.


PubMed | Maratha Mandals Ngh Institute Of Dental Science And Research Center, BDS and H+ Technology
Type: Journal Article | Journal: Journal of clinical and diagnostic research : JCDR | Year: 2014

Toll-like receptors (TLRs) are an important component of immune system. Among them, TLR-2 plays a dominant role in the oral tissues in initiating inflammation in chronic periodontitis. Not many studies have been done on quantitative expression of TLR-2 by using immunofluorescent techniques (IFT) in oral tissues. In this study, the expression and localisation of TLR-2 were detected in gingival tissues of chronic periodontitis patients and healthy individuals.Immuno Fluorescent Technique (IFT) was used for the expression and localization of TLR-2 in gingival tissue samples from 25 chronic periodontitis patients and from 25 healthy controls. Haematoxylin and Eosin staining was also done for all the samples to determine the histological characteristics of the gingival tissue samples.Both healthy and periodontitis gingival tissues expressed TLR-2. We found that the expression level of TLR-2 was higher in all the periodontitis patients than in healthy individuals. We also found out that the expression of TLR-2 was higher in the epithelial cells than in the connective tissue cells.These data suggest a definite involvement of TLR-2 in initiating an inflammatory response in periodontal tissues. More studies are required to define the mechanisms and expression levels of TLR-2 in oral health and diseases.

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