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Kalalbandi V.K.A.,Karnatak University | Seetharamappa J.,Karnatak University | Katrahalli U.,Karnatak University | Bhat K.G.,Maratha Mandals Institute of Dental science and Research Center
European Journal of Medicinal Chemistry | Year: 2014

Series of 1-[(2E)-3-phenylprop-2-enoyl]-1H-benzimidazole derivatives were synthesized and characterized by spectral methods. Among 21 derivatives, single crystals of 3a and 3l were grown and their structural parameters were evaluated. Newly synthesized compounds were screened for anti-tubercular activity and the MIC was determined against Mycobacterium tuberculosis H37Rv by Microplate Alamar Blue Assay (MABA) method. Majority of the compounds exhibited a promising inhibition of M. tuberculosis and the molecules functionalized with electron-donating groups at C-2 carbon of benzimidazole moiety were found to be more active in inhibiting M. tuberculosis. Further, more promising compounds viz., 3b, 3i and 3l were tested for their cytotoxic activity. Compound 3l was found to display excellent activity (IC50 < 10 μg mL -1) with 100% cell lysis at 30 μg mL-1 concentration against A549 (Human lung carcinoma) and 8E5 (Human; Acute Lymphoblastic Leukemia) cell lines. © 2014 Elsevier Masson SAS. All rights reserved.

Mason M.R.,Ohio State University | Nagaraja H.N.,Ohio State University | Camerlengo T.,Ohio State University | Joshi V.,Maratha Mandals Institute of Dental science and Research Center | Kumar P.S.,Ohio State University
PLoS ONE | Year: 2013

Oral infections have a strong ethnic predilection; suggesting that ethnicity is a critical determinant of oral microbial colonization. Dental plaque and saliva samples from 192 subjects belonging to four major ethnicities in the United States were analyzed using terminal restriction fragment length polymorphism (t-RFLP) and 16S pyrosequencing. Ethnicity-specific clustering of microbial communities was apparent in saliva and subgingival biofilms, and a machine-learning classifier was capable of identifying an individual's ethnicity from subgingival microbial signatures. The classifier identified African Americans with a 100% sensitivity and 74% specificity and Caucasians with a 50% sensitivity and 91% specificity. The data demonstrates a significant association between ethnic affiliation and the composition of the oral microbiome; to the extent that these microbial signatures appear to be capable of discriminating between ethnicities. © 2013 Mason et al.

Prabhu N.,KLE University | Keluskar V.,KLE University | Ammanagi R.,Maratha Mandals Institute of Dental science and Research Center | Issrani R.,Saraswati Dental College
International Journal of Perioperative Ultrasound and Applied Technologies | Year: 2013

Background: Hypertension is one of the most common medical problems seen in our dental profession. Nutrient canals are spaces in bones and are considered to be channels that transmit blood vessels and nerves. They are observed more frequently in the mandibular anterior region as radiolucent lines in intraoral periapical radiographs. The radiographically observable nutrient canals have been shown to have an association with various systemic diseases like hypertension. This can be used as an evidence for the detection of hypertensive patients for better diagnosis and treatment decisions. Aim and objectives: The aim of the present study was to evaluate if any correlation exists between nutrient canals and hypertension. Materials and methods: Two hundred patients with hypertension for 1 to 10 years (groups I and II) and 100 age matched controls (group III) were included in the study. The prevalence of nutrient canals in all the groups and with reference to age and gender was observed along with the comparison between intraoral conventional and digital radiography. Results: 1. Differences in the prevalence of nutrient canals between groups I, II and III were found to be statistically significant. 2. In the groups I and II, prevalence of nutrient canals increased with age and also with the duration of hypertension, however it was statistically insignificant. There was no significant difference in prevalence rate among the different age groups in control group. 3. The presence of nutrient canals among the three groups in males and females was statistically significant. 4. Differences in the prevalence of nutrient canals when compared with intraoral conventional and digital radiographic method among all the three groups did not reveal statistically significant results. Conclusion: It is evident from the present study that there is a correlation between prevalence of nutrient canals and hypertension.

Sidhu M.,KLE University | Kale A.D.,KLE University | Kotrashetti V.S.,Maratha Mandals Institute of Dental science and Research Center
Journal of Oral and Maxillofacial Pathology | Year: 2012

Background: Hereditary ectodermal dysplasia is a genetic recessive trait characterized by hypohydrosis, hypotrichosis, and hypodontia. The affected individual show characteristic physiognomy like protruded forehead, depressed nasal bridge, periorbital wrinkling, protruded lips, etc. There is marked decrease in sweat and salivary secretion. Due to skin involvement palm and sole ridge patterns are disrupted. Aim: In this study an attempt has been made to classify the affected members according to the degree of penetrance by pedigree analysis and also study karyotyping for cytogenetics, dermatoglyphic analysis for the various ridge patterns and variations in the number of sweat glands by sweat pore analysis in affected individuals. Materials and Methods: A total of five families who were affected with ectodermal dysplasia were considered. Pedigree analysis was drawn up to three generation by obtaining history. Dermatoglyphics and sweat pore analysis was done by obtaining palm and finger print impression using stamp pad ink. Karyotyping was done by collecting 3-5 ml peripheral blood. Karyotyping was prepared using lymphocyte culture. Chromosomes were examined at 20 spreads selected randomly under 100 magnification. Results were analyzed by calculating mean values and percentage was obtained. Results: Karyotyping did not show any abnormalities, dermatoglyphic analysis and sweat pore counts showed marked variations when compared with normal. Moreover, pedigree analysis confirmed the status of the disease as that of the recessive trait. Conclusion: Large number of affected patients needs to be evaluated for dermatoglypic analysis. Genetic aspect of the disease needs to be looked into the molecular level in an attempt to locate the gene locus responsible for ectodermal dysplasia and its manifestation.

Kotrashetti V.S.,Maratha Mandals Institute of Dental science and Research Center | Mallapur M.D.,Jn Medical College
Journal of Forensic and Legal Medicine | Year: 2016

Facial reconstruction is a technique used in forensic anthropology to identify an unknown person. Various methods used for facial reconstruction are drawings, sculpture and computer aided image building which is mainly based on facial soft tissue thickness measurement. Several methods have been established for measuring facial soft tissue thickness (FSTT) with each one having certain limitations. There is limited data available on FSTT among South Indian population. Hence the present study was ventured to determine the FSTT among South Indian adults and also to find FSTT difference between male and female. 308 subjects of South Indian origin (18-27years) having full set of permanent dentition who require orthodontic treatment were included in the study. Subjects were assessed for Body Mass Index (BMI) and diagnostic digital x-ray of lateral cephalogram (LC), Lateral oblique (LO) view and posterior-anterior (PA) view was obtained. The digital image was transferred to Adobe Photoshop CS4 software and 23 different soft tissue points were measured. Mean FSTT was more in males compared to females except for three landmarks. Statistically significant difference was observed in 20 landmarks when height and weight was compared in males, whereas in females only 12 landmarks showed significant difference. BMI showed good correlation with FSTT in both males and females, which was confirmed by linear regression. The best regressor in terms of estimating FSTT in association with age/sex/BMI were nasion, sub nasale, labial superioris, labrale inferius, gnathion, inferior border of zygomatic, right and left gonion. Stepwise discriminant analysis using all variables showed 94.8% of overall accuracy in sex determination. The observation of present study suggests that LO and PA view along with LC gives information regarding mean FSTT among South Indian population. Even though BMI plays a dominant role in determining FSTT, but age, sex, height and weight should also be considered with care while facial reconstruction. Additionally the present regression equation contributes towards increase in the specificity of the tissue depths and can be used in real cases by allowing the practitioners to calculate individual tissue depth. © 2016 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.

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