Braga, Portugal


Braga, Portugal
Time filter
Source Type

Sousa R.M.O.F.,University of Minho | Rosa J.S.,University of The Azores | Cunha A.C.,University of Minho | Fernandes-Ferreira M.,University of Porto | Fernandes-Ferreira M.,MAPPROD Lda
Journal of Pest Science | Year: 2017

Snails of the family Lymnaeidae are an essential link in the transmission of zoonotic diseases. Radix peregra is a European freshwater snail and a susceptible intermediate host of Fasciola hepatica, the causing agent of fascioliasis. Essential oils (EOs) extracted from Anethum graveolens (dill), Cuminum cyminum (cumin), Foeniculum vulgare var. vulgare (bitter fennel) and Petroselinum crispum (plain leaf parsley) were characterized by GC and GC–MS. Seven EOs and 11 constituents were first screened through a single-dose bioassay against R. peregra (10 mg L−1 for juveniles and 50 mg L−1 for egg masses and mature snails). EOs from parsley, cumin and bitter fennel (leaves plus stems) were highly active towards eggs and adults at 50 mg L−1. Subsequently, dose and time–lethality bioassays were performed against adults to determine lethal parameters (LC50;90 and LT50;90). Estimated 48 h LC50s varied from 13.7 to 46.5 mg L−1, with P. crispum fruits EO exhibiting the most significant activity. EOs from cumin fruits and bitter fennel infrutescences, and cuminaldehyde, were the most time-effective treatments when assessed by continuous exposure (LT50 for a 50 mg L−1 dose = 15.1, 19.3 and 19.5 h, respectively). A short-time exposure (8 h) to bitter fennel EOs was effective for the control of adults (LT50 ≤25 h). The present study uncovers the potential of four well-known Apiaceae species as natural sources of biomolluscicides. © 2017 Springer-Verlag Berlin Heidelberg

Sousa R.M.O.F.,University of Minho | Rosa J.S.,University of The Azores | Oliveira L.,University of The Azores | Cunha A.,University of Minho | And 3 more authors.
Journal of Agricultural and Food Chemistry | Year: 2013

Essential oils (EOs) from four Apiaceae species and 11 pure compounds were evaluated for their antifeedant, growth inhibitory, and insecticidal activities against Pseudaletia unipuncta (Lepidoptera: Noctuidae) fourth-instar larvae. EOs from Foeniculum vulgare subsp. vulgare var. vulgare, Anethum graveolens, Petroselinum crispum, and Cuminum cyminum were characterized by gas-chromatography (GC) and mass spectrometry. Anti-insect activity varied according to plant specie/composition, type, and exposure period. EOs from P. crispum and A. graveolens fruits, trans-anethole and cuminaldehyde, exerted acute effects on larvae feeding and growth (FDI and GI > 70%). A. graveolens, C. cyminum, and F. vulgare EOs and some of their constituents were effective by fumigation (≥80%). Satisfactory contact toxicities (>70%) were observed for five compounds and all EOs, except F. vulgare EOs, when tested by the filter paper impregnation method. For the most active EOs/compounds, dose-dependent toxicity was determined and inverse relationships of LC50 with time were established. © 2013 American Chemical Society.

Ferreira P.,University of Minho | Cardoso T.,University of Minho | Ferreira F.,University of Minho | Fernandes-Ferreira M.,Center for the Research and Technology of Agro Environmental and Biological science | And 4 more authors.
FEMS Yeast Research | Year: 2014

Mentha piperita (MP), also known as peppermint, is an aromatic and medicinal plant widely used in the food industry, perfumery and cosmetic, pharmacy and traditional medicine. Its essential oil (EO) displays antimicrobial activity against a range of bacteria and fungi. In this study, we found that MP EO lethal cytotoxicity is associated with increased levels of intracellular reactive oxygen species, mitochondrial fragmentation and chromatin condensation, without loss of the plasma membrane integrity, indicative of an apoptotic process. Overexpression of cytosolic catalase and superoxide dismutases reverted the lethal effects of the EO and of its major component menthol. Conversely, deficiency in Sod1p (cytosolic copper-zinc-superoxide dismutase) greatly increased sensitivity to both agents, but deficiency in Sod2p (mitochondrial manganese superoxide dismutase) only induced sensitivity under respiratory growth conditions. Mentha piperita EO increased the frequency of respiratory deficient mutants indicative of damage to the mitochondrial genome, although increase in mitochondrial thiol oxidation does not seem to be involved in the EO toxicity. Essential oil from Mentha piperata induces both cytosolic and mitochondrial ROS-mediated damage in Saccharomyces cerevisiae, resulting in apoptotic cell death that can be reverted by increased expression of Sod1p, Sod2p and Ctt1p. © 2014 Federation of European Microbiological Societies.

Sousa R.M.O.F.,University of Minho | Rosa J.S.,University of The Azores | Silva C.A.,University of Minho | Almeida M.T.M.,University of Minho | And 5 more authors.
Journal of Pest Science | Year: 2015

Plant-based products, namely essential oils (EOs), are environmentally friendly alternatives for the control of disease vectors, hosts and/or parasites. Here, we studied the general toxicity and biopesticidal potential of EOs and phenylpropanoids from Foeniculum vulgare var. vulgare (bitter fennel), a perennial plant well adapted to temperate climates. EO/compound toxicity was tested against a freshwater snail and potential intermediate host of Fasciola hepatica (Radix peregra), a mosquito and former European malaria vector (Anopheles atroparvus) and one of the most damaging plant-parasitic nematodes, the root-knot nematode (Meloidogyne javanica). Lethal concentrations (LC50; LC90) of EOs (infrutescences/stems with leaves) and compounds were calculated by probit analysis. All displayed noteworthy activity against R. peregra adults (LC50 21–39 µg ml−1) and A. atroparvus larvae (LC50 16–56 µg ml−1). trans-Anethole revealed acute nematicidal activity after 24 and 48 h (LC50 310 and 249 µg ml−1, respectively), and estragole (1,000 µg ml−1) showed some effectiveness against M. javanica hatching and juveniles after 15 days. Plant and EO yields were determined to evaluate the bitter fennel productivity. The chemical composition of the EOs was analyzed by gas chromatography coupled to mass spectrometry. EOs extracted from whole plants, infrutescences and stems with leaves were characterized by estragole-dominant profiles (28–65 %), considerable amounts of phellandrene (10–34 %) and fenchone (6–16 %), and minor trans-anethole contents (1–4 %). Although additional toxicological studies against nontarget organisms are required, our study demonstrates that bitter fennel is a productive source of molluscicides and larvicides, and thus a potential sustainable biological agent to control particular host species, namely freshwater snails and mosquitoes. © 2014, Springer-Verlag Berlin Heidelberg.

Guedes A.P.,University of Minho | Franklin G.,University of Minho | Fernandes-Ferreira M.,University of Minho | Fernandes-Ferreira M.,University of Porto | Fernandes-Ferreira M.,Mapprod Lda.
Phytochemistry Reviews | Year: 2012

Phytochemical composition of Hypericum genus has been investigated for many years. In the recent past, studies on the essential oils (EO) of this genus have been progressing and many of them have reported interesting biological activities. Variations in the EO composition of Hypericum species influenced by seasonal variation, geographic distribution, phenological cycle and type of the organ in which EO are produced and/or accumulated have also been reported. Although many reviews attributed to the characterization as well as biological activities of H. perforatum crude extracts have been published, no review has been published on the EO composition and biological activities of Hypericum species until recently (Crockett in Nat Prod Commun 5(9):1493-1506, 2010; Bertoli et al. in Global Sci Books 5:29-47, 2011). In this article, we summarize and update information regarding the composition and biological activities of Hypericum species EO. Based on experimental work carried out in our laboratory we also mention possible biotechnology approaches envisaging EO improvement of some species of the genus. © Springer Science+Business Media B.V. 2011.

Sousa R.M.O.F.,University of Minho | Rosa J.S.,University of The Azores | Oliveira L.,University of The Azores | Cunha A.,University of Minho | And 3 more authors.
Industrial Crops and Products | Year: 2015

The inhibitory activities of Anethum graveolens, Cuminum cyminum, Foeniculum vulgare and Petroselinum crispum Essential Oils (EOs), plus eleven volatile compounds, on embryonic development of a phytophagous pest, Pseudaletia unipuncta (Lepidoptera: Noctuidae) were evaluated. Lethal and sub-lethal effects of some treatments during the postembryonic development were also monitored. The higher effectiveness to inhibit eggs hatching was achieved with P. crispum fruit EO and its two main compounds (apiole and myristicin). F. vulgare EOs, as well as the bioactive compound azadirachtin (1mgmL-1) and the volatiles trans-anethole, estragole, (+)-fenchone, α-pinenes, (-)-β-pinene and γ-terpinene (15mgmL-1), had negligible inhibitory effects on larvae emergence, although, some induced post-exposure mortality during larval phase. A. graveolens EO from leaves and stems, and six compounds, including azadirachtin, extended significantly the duration of larval development from 1.9 to 6.5 days. (+)-Fenchone and (-)-β-pinene reduced significantly the oviposition potential of females, by 46% and 33%, respectively.Additionally, a quantitative nutritional approach was conducted in order to assess adverse effects of EOs and compounds on 4th instar larvae growth and metabolism. After 72. h of feeding with treated corn leaves (0.7. mg/leaf), P. crispum fruit EO (Pc-F) and azadirachtin (0.04. mg/leaf) caused significant adverse effects on larva nutrition (lower consumption and Relative Consumption Rate) and growth (weight loss and negative Relative Growth Rate), followed by trans-anethole and cuminaldehyde. As verified for the positive control azadirachtin, trans-anethole and cuminaldehyde had negative and significant influences on the Relative Metabolic Rate (RMR) and on the efficiency of conversion of food to biomass (measured as ECI and ECD), with consequent higher Metabolic Costs (MC) to the larvae.P. crispum EO from fruits revealed great potential as a controlling agent of P. unipuncta in early life stages, acting as a hatching inhibitor, larvicide and phagodeterrent. © 2014 Elsevier B.V.

Rainha N.,University of The Azores | Lima E.,University of The Azores | Baptista J.,University of The Azores | Fernandes-Ferreira M.,Center for the Research and Technology of Agro Environmental and Biological science | And 2 more authors.
Natural Product Research | Year: 2013

This study reports the first quantification study of pseudohypericin (PsHyp) and hypericin (Hyp) in Hypericum undulatum Schousb. ex Willd in vitro cultures developed by a Portuguese company. Both compounds were quantified by high-performance liquid chromatography and their levels were compared with those in commercial samples of Hypericum perforatum. PsHyp was found to be the major naphthodianthrone of H. undulatum, with an average ratio of 3.73:1 compared to Hyp. Significant statistical differences were found between the content of Hyp and PsHyp in H. undulatum regenerated shoots compared to in vivo samples. The mean concentration of total Hyps varied from 178.41 to 358.93 g g-1 dry extract in H. undulatum regenerated shoots, which is on average two to three times less than naphthodianthrone levels found in H. undulatum in vivo and H. perforatum commercial samples. However, none of the analysed samples presented the levels of Hyps required by the European and United States Pharmacopoeias. © 2013 Taylor & Francis.

Rainha N.,University of The Azores | Koci K.,New University of Lisbon | Coelho A.V.,New University of Lisbon | Lima E.,University of The Azores | And 4 more authors.
Phytochemistry | Year: 2013

LC-UV and LC-MS analysis were used to study the phenolic composition of water extracts of Hypericum undulatum (HU) shoot cultures and wild-growing (WG) plants. Total phenolic content (TPC), determined using the Folin-Ciocalteu assay, and the antioxidant activity measured by two complementary methods were also performed for each sample. Mass spectrometry revealed several phenolics acids with quinic acid moieties, flavonols, mostly quercetin, luteolin and apigenin glycosides, flavan-3-ols (catechin and epicatechin) and the xanthonoid mangiferin. Differences in phenolic composition profile and TPC were found between the samples. The major phenolic in HU culture-growing (CG) samples is chlorogenic acid, followed by epicatechin, quercitrin and isoquercitrin. The WG plants presents hyperoside as the main phenolic, followed by isoquercitrin, chlorogenic acid and quercetin. The TPC and antioxidant activity were higher in samples from WG plants. © 2012 Elsevier Ltd. All rights reserved.

Loading MAPPROD Lda collaborators
Loading MAPPROD Lda collaborators