Manose Health and Beauty Research Center

Chiang Mai, Thailand

Manose Health and Beauty Research Center

Chiang Mai, Thailand
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Sainakham M.,Chiang Mai University | Manosroi A.,Chiang Mai University | Manosroi A.,Manose Health and Beauty Research Center | Manosroi A.,North - Chiang Mai University | And 5 more authors.
Drug Delivery | Year: 2016

The in vivo anticancer activity and stability of liposomes encapsulated with semi-purified Job’s tear (Coix lacryma-jobi Linn.) extracts (S5L), prepared by supercritical carbon dioxide fluid technique, on human colon adenocarcinoma (HT29) xenografted mice were investigated. For the stability and the physicochemical characteristics, S5L showed a high stability of pH, good dispersibility, small particle size and stable zeta potential. Liposomes can protect linoleic acid in the extract comparing with the free S5. S5L kept at 4 °C for 3 months showed the highest linoleic acid content of 63.50%, whereas at 45 °C, the lowest linoleic acid content of 42.66% was observed. The anticancer activity and toxicity on xenografted mice were observed for 14 days. At the end of the experiment, the relative tumor volume (RTV) in the S5L-treated xenografted mice showed a significant RTV reduction. The high dose of S5 and S5L were potent with the highest inhibition of tumor growth of 48.67 and 54.75%, which was 86.94% and 97.81% of 5-fluorouracil, respectively. The apoptotic activity was shown in xenografted mice treated with S5 at medium and high dose, S5L, 5-fluorouracil and commercial product. All treated xenografted mice showed no toxic signs and symptoms, abnormality of internal organs histopathology and blood chemistry. This study has demonstrated the high physicochemical stability of liposomes encapsulated with semi-purified Job’s tear extract and their potent anticancer activity on human colon adenocarcinoma xenografted model with the potential for further development to anticolon cancer drug. © 2016 Informa UK Limited, trading as Taylor & Francis Group.


Manosroi A.,Chiang Mai University | Manosroi A.,Manose Health and Beauty Research Center | Manosroi A.,North - Chiang Mai University | Sainakham M.,Chiang Mai University | And 7 more authors.
Journal of Ethnopharmacology | Year: 2016

Ethonopharmacological relevances Job's tears (Coix lacryma-jobi Linn.) is commonly used as an herbal medicine. The low incidence of cancer has been observed in the area of China where regularly consume Job's tears. The effects of preparation methods of Job's tears on anti-cancer activities were investigated. Potent in vitro anti-proliferative, apoptotic and anti-oxidative activities of semi-purified Job's tears extracts from different preparation methods on 5 human cancer cell lines comparing with standards and commercial product were observed. Aim of study To study the anti-proliferative, apoptotic and anti-oxidative activities of semi-purified Job's tear extracts from different preparation methods on 5 human cancer cell lines. Materials and methods The crude methanolic extracts of non-cooked, steamed and roasted Job's tears cultivars were prepared and further semi-purified by liquid-liquid extraction techniques. Both of crude and semi-purified extracts were tested for anti-proliferative, apoptotic induction, anti-oxidative activities and phytochemicals content. Results The highest yields of crude and-purified extracts were 4.60% and 1.46%, respectively. In crude extracts, the steamed whole Thai Black Loei Job's tears (W-TBL-S1) extract showed the highest anti-proliferative activity in mouth epidermal carcinoma cell (KB) at the IC50 of 43.61±0.76 μg/ml (0.005 folds of doxorubicin), whereas the roasted whole Laos White Loei Job's tears (W-LWL-R2) extract showed the highest apoptotic activity in cervical adenocarcinoma (HeLa) at 21.52±1.50% (0.22 and 15.05 folds of doxorubicin and commercial product, respectively). After liquid-liquid extraction, almost all of the semi-purified extracts showed increases in anti-proliferative activity. Ethyl acetate fraction of the roasted whole Laos White Loei Job's tears (W-LWL-R2) showed the highest anti-proliferative activity in HeLa cell at the IC50 of 0.97±0.82 μg/ml (7.82 and 45.39 folds of doxorubicin and crude extract, respectively) and apoptotic activity of 18.77±6.31% (0.19 folds of doxorubicin). The commercial product showed no anti-proliferative activity in all cell lines but induced apoptosis in HeLa cell at 1.43±0.34%. The butanol and hexane soluble fractions of the roasted whole of Laos White Loei Job's tears (W-LWL-R2) showed the highest free radical scavenging (SC50) and metal chelating activity (MC50) of 0.31±0.06 mg/ml (0.64 folds of ascorbic acid) and 0.08±0.01 mg/ml (6.37 folds of EDTA), respectively. All ethyl acetate fractions contain high content of carotenoid and tannin, whereas the hexane soluble fraction of the roasted hull of Laos Black Loei Job's tears (H-LBL-R1) showed the highest linoleic acid content of 8.09±0.74% w/w. Conclusions This study has demonstrated the potent anti-cancer activity of the semi-purified extracts from roasted Job's tears with high potential for further development to modern anti-cancer drug. © 2016 Elsevier Ireland Ltd. All rights reserved.


Leelarungrayub J.,Chiang Mai University | Manorsoi J.,Manose Health and Beauty Research Center | Manorsoi J.,North - Chiang Mai University | Manorsoi A.,Manose Health and Beauty Research Center | Manorsoi A.,North - Chiang Mai University
International Journal of Nanomedicine | Year: 2017

Objective: The aim of this study was to evaluate the antioxidant and anti-inflammatory activities of Plai oil–encapsulated niosomes (Zingiber cassumunar Roxb.) on inflamed subcutaneous Wistar rat skin by therapeutic ultrasound. Methods: Pure oil from Plai rhizomes was extracted by steam distillation, and antioxidant activities were determined by 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay. Bioactive compounds were analyzed by gas chromatography-mass spectrometry. Niosome particles containing Plai oil were prepared by chloroform film method with sonication before testing for anti-inflammatory activity on locally inflamed subcutaneous rat skin after inducement from lipopolysaccharide with ultrasound once a day for 3 days. Skin temperatures and blood flow were evaluated. Results: Plai oil presented antioxidant activity that inhibited 2,2-diphenyl-1-picrylhydrazyl radicals. Four active compounds found in the essential oil were sabinene, γ-terpinene, terpinene-4-ol, and (E)-1-(3,4-dimethyoxy phenyl) butadiene. Application of ultrasound (0.2 W/cm2, 20%, 3 min) with gel containing Plai oil–encapsulated niosomes decreased skin temperature and blood flow to the lowest level compared to the application of neurofen drug or gel-based control. Conclusion: Plai oil, which consists of four main bioactive compounds and possesses antioxidant and anti-inflammatory activities, can be applied against local subcutaneous inflammation when used with therapeutic ultrasound via entrapped niosomes. © 2017 Leelarungrayub et al.


Manosroi A.,Chiang Mai University | Manosroi A.,Manose Health and Beauty Research Center | Manosroi A.,North - Chiang Mai University | Tangjai T.,Chiang Mai University | And 11 more authors.
Drug Delivery | Year: 2016

This study aimed to investigate the synergistic effect of trans-activator of transcription (Tat) and niosomes for the improvement of hypoglycemic activity of orally delivered human insulin. The elastic anionic niosomes composing of Tween 61/cholesterol/dicetyl phosphate/sodium cholate at 1:1:0.05:0.02 molar ratio loaded with insulin–Tat mixture (1:3 molar ratio) was prepared. Deformability of the elastic anionic niosomes decreased after loaded with the mixture of 1.35 times. For the in vitro release, the insulin (T10 = 4 h) loaded in the elastic anionic niosomes indicated the slower release rate than insulin in the mixture (T10 = 3 h) loaded in niosomes. At room temperature (30 ± 2 °C), the mixture loaded in elastic anionic niosomes was more chemical stable than the free mixture of 1.3, 1.4 and 1.7 times after stored for 4, 8 and 12 weeks, respectively. Oral administration in the alloxan-induced diabetic mice of the mixture loaded in elastic anionic niosomes with the insulin doses at 25, 50 and 100 IU/kg body weight indicated significant hypoglycemic activity with the percentage fasting blood glucose reduction of 1.95, 2.10 and 2.10 folds of the subcutaneous insulin injection at 12 h, respectively. This study has demonstrated the synergistic benefits of Tat and elastic anionic niosomes for improving the hypoglycemic activity of the orally delivered human insulin as well as the stability enhancement of human insulin when stored at high temperature. The results from this study can be further developed as an effective oral insulin delivery. © 2016 Informa UK Limited, trading as Taylor & Francis Group.


Manosroi J.,Manose Health and Beauty Research Center | Manosroi J.,Chiang Mai University | Chankhampan C.,Manose Health and Beauty Research Center | Foe K.,Widya Mandala Surabaya Catholic University | And 4 more authors.
Chiang Mai Journal of Science | Year: 2016

This study aimed to increase the solubility of indomethacin in water by complexation it with hydroxypropyl-β-cyclodextrin (HPβCD). Phase-solubility analysis was used to investigate interactions in aqueous solution between HPβCD and indomethacin. Equimolar indomethacin-HPβCD solid systems were prepared by four different methods including physical mixtures (PM), kneading (KN), coevaporation (COE) and freeze-drying (COL) methods. The complex was characterized by infrared spectroscopy, differential scanning calorimetry, X-ray diffractometry, thin layer chromatography and dissolution rates. The complexation efficiency of indomethacin-HPβCD was determined spectrophotometrically. The solubility of indomethacin increased linearly as the concentration of HPβCD increased. This indicated a feature of the AL-type complex that the water-soluble complexes existed in the solution. The average of apparent 1:1 stability constant of the complex (K1:1) at 30°C was 340 M-1. The KN and COE methods formed partial inclusion complexes, whereas the COL method gave complete complexation. The dissolution rates of indomethacin increased when complexed with HPβCD. HPβCD complexation of an ionized drug molecule by the COL method exhibited the highest dissolution rate of indomethacin [the dissolution efficiency after 90 min (DE90) at 61.7±0.9% and t50% of 13 min., while the uncomplexed indomethacin showed DE90 at 15.4±0.1% and t50% more than 90 min. The COL process was the best method because of the high content and dissolution rate of the drug. It is also the simple method to prepare the inclusion complexes. The result from this study has suggested the dissolution rate enhancement of indomethacin by the simple complexaion method with HPβCD. © 2016, Chiang Mai Journal of Science. All rights reserved.


Manosroi A.,Chiang Mai University | Manosroi A.,Manose Health and Beauty Research Center | Akazawa H.,Chiang Mai University | Akazawa H.,Nihon University | And 7 more authors.
Pharmaceutical Biology | Year: 2015

Thai/Lanna medicinal plant recipes have been used for the treatment of several diseases including oral and cervical cancers. Objective: To investigate anti-proliferative activity on human cervical (HeLa) and oral (KB) cancer cell lines of medicinal plants selected from Thai/Lanna medicinal plant recipe database "MANOSROI III". Materials and methods: Twenty-three methanolic plant crude extracts were tested for phytochemicals and anti-proliferative activity on HeLa and KB cell lines for 24h by the sulforhodamine B (SRB) assay at the doses of 1×10-1×10-6mg/ml. The nine extracts with the concentrations giving 50% growth inhibition (GI50) lower than 100μg/ml were further semi-purified by liquid/liquid partition in order to evaluate and enhance the anti-proliferative potency. Results: All extracts contained steroids/triterpenoids, but not xanthones. The methanolic extracts of Gloriosa superba L. (Colchinaceae) root and Albizia chinensis (Osbeck) Merr. (Leguminosae-Mimosoideae) wood gave the highest anti-proliferative activity on HeLa and KB cell lines with the GI50 values of 0.91 (6.0-and 0.31-fold of cisplatin and doxorubicin) and 0.16μg/ml (28.78-and 82.29-fold of cisplatin and doxorubicin), respectively. Hexane and methanol-water fractions of G. superba exhibited the highest anti-proliferative activity on HeLa and KB cell lines with the GI50 values of 0.15 (37-and 1.9-fold of cisplatin and doxorubicin) and 0.058μg/ml (77.45-and 221.46-fold of cisplatin and doxorubicin), respectively. Discussion and conclusion: This study has demonstrated the potential of plants selected from MANOSROI III database especially G. superba and A. chinensis for further development as anti-oral and cervical cancer agents. © 2015 Informa Healthcare USA, Inc.


Manosroi A.,Manose Health and Beauty Research Center | Manosroi A.,Chiang Mai University | Sainakham M.,Manose Health and Beauty Research Center | Sainakham M.,Chiang Mai University | And 5 more authors.
Saudi Journal of Biological Sciences | Year: 2016

The whole seed (W), endosperm (E) and hull (H) of five cultivars of Job's tears (Coix lachryma-jobi Linn. var. ma-yuen Stapf) including Thai Black Phayao, Thai Black Loei, Laos Black Loei, Laos White Loei and Laos Black Luang Phra Bang were processed before solvent extraction by non-cooking, roasting, boiling and steaming Each part of the Job's tears was extracted by the cold and hot process by refluxing with methanol and hexane. The total of 330 extracts included 150 methanol extracts and 180 hexane extracts were investigated for anti-proliferative activity on human colon adenocarcinoma cell line (HT-29) by the sulforhodamine B (SRB) assay. The extracts which gave high anti-proliferative activity were tested for apoptotic activity by acridine orange and ethidium bromide double staining and anti-oxidative activities including free radical scavenging and lipid peroxidation inhibition activities. The extract from the hull of Thai Black Loei roasted before extracting by hot methanol (M-HTBL-R2) showed the highest anti-proliferative activity on HT-29 with the IC50 values of 11.61 ± 0.95 μg/ml, while the extract from the non-cooked hull of Thai Black Loei by cold methanol extraction (M-HTBL-N1) gave the highest apoptosis (8.17 ± 1.18%) with no necrosis. In addition, M-HTBL-R2 and M-HTBL-N1 indicated free radical scavenging activity at the SC50 values of 0.48 ± 0.12 and 2.47 ± 1.15 mg/ml, respectively. This study has demonstrated the anti-colorectal cancer potential of the M-HTBL-R2 and M-HTBL-N1 extracts. © 2015 The Authors.


Manosroi J.,Manose Health and Beauty Research Center | Manosroi J.,Chiang Mai University | Chankhampan C.,Manose Health and Beauty Research Center | Kumguan K.,Chiang Mai University | And 3 more authors.
Pharmaceutical Biology | Year: 2015

Context: Cleistocalyx nervosum (DC.) Kosterm. var. paniala (Roxb.) J. Parn. & Chantaran. (Myrtaceae) or Ma Kiang contains high amounts of phenolic compounds. Antioxidant activity of its fruit and seed has been investigated. However, limited available information concerning the biological activities of its leaves has been reported. Objective: To investigate the in vitro anti-aging potential of young and old leaves of Ma Kiang. Materials and methods: Ma Kiang leaves were extracted using water, methanol, and chloroform as the solvents by cold (sonication) and hot (boiling) processes. The extracts were determined for total phenolic and flavonoid contents. The extracts (at 0.001-10mg/ml) were tested for antioxidative and tyrosinase inhibition activities using a colorimetric method. The cytotoxicity of extracts (at 0.0001-1mg/ml) was determined with human skin fibroblasts. Also, the extracts at 0.001, 0.01, and 0.1mg/ml which showed no toxicity were tested for MMP-2 inhibition. Results: The cold methanol extract of the old leaves showed the highest total phenolic and flavonoid contents of 511.44±18.23μg GAE/mg and 262.96±2.98μg QE/mg, respectively. This extract also gave high free radical scavenging, lipid peroxidation inhibition, and tyrosinase inhibition activities with SC50, IPC50, and IC50 values of 0.02±0.004, 0.23±0.13, and 0.02±0.006mg/ml, respectively. The extract at 0.1mg/ml exhibited the highest MMP-2 inhibition of 91.14±1.67%. Discussion and conclusion: The anti-aging potential of the cold methanol extract from old leaves of Ma Kiang can be further developed as an anti-aging agent. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted.


Manosroi J.,Chiang Mai University | Manosroi J.,Manose Health and Beauty Research Center | Jantrawut P.,Chiang Mai University | Manosroi W.,Chiang Mai University | And 3 more authors.
Chiang Mai Journal of Science | Year: 2015

Sesamin is the primary compound in sesame seed oil which has been used in ayurvedic treatment for hair loss and canities. Sesamin was isolated, identified and investigated for antioxidative activities (including DPPH radical scavenging, lipid peroxidation inhibition and metal ion chelating activities), 5α-reductase inhibition in DU-145 and melanogenesis activities on B16F10 melanoma. Sesamin at the concentration of 0.001-10 mg/ml showed weak DPPH scavenging activity (the SC50 value of 109.90 ± 6.16 mg/ml), but quite high lipid peroxidation inhibition and metal ion chelating activities (the IPC50 value of 0.83 ± 0.54 and the CC50 value of 1.83 ± 0.90 mg/ml, respectively). At 0.1 mg/ml of sesamin demonstrated high 5α-reductase inhibition activity at 37.38±4.26% of the control, which was higher than the standard finasteride of 1.54 times, but lower than the standard dutasteride of 1.27 times. It gave high melanin and tyrosinase contents of 26.63±3.21 and 39.42±8.30%, respectively, but lower than theophylline of about 1.04 and 1.05 times, respectively. Although sesamin exhibited all investigated activities lower than the standards, the traditional use of sesame seeds containing sesamin for hair cosmetics has been confirmed, which will be beneficial for the further development as anti-hair loss and anti-canities products. © 2015 Chiang Mai University. All rights reserved.


Manosroi A.,Chiang Mai University | Manosroi A.,Manose Health and Beauty Research Center | Akazawa H.,Chiang Mai University | Akazawa H.,Nihon University | And 7 more authors.
Journal of Ethnopharmacology | Year: 2015

Ethnopharmacological relevance: Thai/Lanna region has its own folklore wisdoms including the traditional medicinal plant recipes. Thai/Lanna medicinal plant recipe database "MANOSROI III" has been developed by Prof. Dr. Jiradej Manosroi. It consists of over 200,000 recipes for all diseases including cancer. To investigate the anti-proliferative and apoptotic activities on human colon cancer cell line (HT-29) as well as the cancer cell selectivity of the methanolic extracts (MEs) and fractions of the 23 selected plants from the "MANOSROI III" database. Materials and methods: The 23 selected plants were extracted with methanol under reflux and evaluated for their anti-proliferative activity by sulforhodamine B assay. The 5 plants (Gloriosa superba, Caesalpinia sappan, Fibraurea tinctoria, Ventilago denticulata and Psophocarpus tetragonolobus) with potent anti-proliferative activity were fractionated by liquid-liquid partition to give 4 fractions including each hexane (HF), methanol-water (MF), n-butanol (BF) and water (WF) fractions. They were tested for antiproliferative activity and cancer cell selectivity. The ME and fractions of G. superba which showed potent anti-proliferative activity were further examined for morphological changes and apoptotic activities by acridine orange (AO)/ethidium bromide (EB) staining. Results: The ME of G. superba root showed active with the highest anti-proliferative activity at 9.17 and 1.58 folds of cisplatin and doxorubicin, respectively. After liquid-liquid partition, HF of V. denticulata, MFs of F. tinctoria, V. denticulata and BF of P. tetragonolobus showed higher anti-proliferative activities than their MEs. The MF of G. superba indicated the highest anti-proliferative activity at 7.73 and 1.34 folds of cisplatin and doxorubicin, respectively, but only 0.86 fold of its ME. The ME and HF, MF and BF of G. superba and MF of F. tinctoria demonstrated high cancer cell selectivity. At 50 μg/ml, ME, HF, MF and BF of G. superba demonstrated higher apoptotic activities than the two standard drugs. Conclusions: This present study has not only confirmed the traditional use of the Thai/Lanna medicinal plant recipes for cancer treatments, but also the potential of the selected plant, G. superba for the further development as a modern anti-cancer drug. © 2014 Elsevier Ireland Ltd. All rights reserved.

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