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Manosroi J.,Chiang Mai University | Manosroi J.,Manose Health and Beauty Research Center | Jantrawut P.,Chiang Mai University | Manosroi W.,Chiang Mai University | And 3 more authors.
Chiang Mai Journal of Science | Year: 2015

Sesamin is the primary compound in sesame seed oil which has been used in ayurvedic treatment for hair loss and canities. Sesamin was isolated, identified and investigated for antioxidative activities (including DPPH radical scavenging, lipid peroxidation inhibition and metal ion chelating activities), 5α-reductase inhibition in DU-145 and melanogenesis activities on B16F10 melanoma. Sesamin at the concentration of 0.001-10 mg/ml showed weak DPPH scavenging activity (the SC50 value of 109.90 ± 6.16 mg/ml), but quite high lipid peroxidation inhibition and metal ion chelating activities (the IPC50 value of 0.83 ± 0.54 and the CC50 value of 1.83 ± 0.90 mg/ml, respectively). At 0.1 mg/ml of sesamin demonstrated high 5α-reductase inhibition activity at 37.38±4.26% of the control, which was higher than the standard finasteride of 1.54 times, but lower than the standard dutasteride of 1.27 times. It gave high melanin and tyrosinase contents of 26.63±3.21 and 39.42±8.30%, respectively, but lower than theophylline of about 1.04 and 1.05 times, respectively. Although sesamin exhibited all investigated activities lower than the standards, the traditional use of sesame seeds containing sesamin for hair cosmetics has been confirmed, which will be beneficial for the further development as anti-hair loss and anti-canities products. © 2015 Chiang Mai University. All rights reserved. Source


Manosroi J.,Manose Health and Beauty Research Center | Manosroi J.,Chiang Mai University | Chankhampan C.,Manose Health and Beauty Research Center | Kumguan K.,Chiang Mai University | And 3 more authors.
Pharmaceutical Biology | Year: 2015

Context: Cleistocalyx nervosum (DC.) Kosterm. var. paniala (Roxb.) J. Parn. & Chantaran. (Myrtaceae) or Ma Kiang contains high amounts of phenolic compounds. Antioxidant activity of its fruit and seed has been investigated. However, limited available information concerning the biological activities of its leaves has been reported. Objective: To investigate the in vitro anti-aging potential of young and old leaves of Ma Kiang. Materials and methods: Ma Kiang leaves were extracted using water, methanol, and chloroform as the solvents by cold (sonication) and hot (boiling) processes. The extracts were determined for total phenolic and flavonoid contents. The extracts (at 0.001-10mg/ml) were tested for antioxidative and tyrosinase inhibition activities using a colorimetric method. The cytotoxicity of extracts (at 0.0001-1mg/ml) was determined with human skin fibroblasts. Also, the extracts at 0.001, 0.01, and 0.1mg/ml which showed no toxicity were tested for MMP-2 inhibition. Results: The cold methanol extract of the old leaves showed the highest total phenolic and flavonoid contents of 511.44±18.23μg GAE/mg and 262.96±2.98μg QE/mg, respectively. This extract also gave high free radical scavenging, lipid peroxidation inhibition, and tyrosinase inhibition activities with SC50, IPC50, and IC50 values of 0.02±0.004, 0.23±0.13, and 0.02±0.006mg/ml, respectively. The extract at 0.1mg/ml exhibited the highest MMP-2 inhibition of 91.14±1.67%. Discussion and conclusion: The anti-aging potential of the cold methanol extract from old leaves of Ma Kiang can be further developed as an anti-aging agent. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted. Source


Manosroi A.,Chiang Mai University | Manosroi A.,Manose Health and Beauty Research Center | Akazawa H.,Chiang Mai University | Akazawa H.,Nihon University | And 7 more authors.
Pharmaceutical Biology | Year: 2015

Thai/Lanna medicinal plant recipes have been used for the treatment of several diseases including oral and cervical cancers. Objective: To investigate anti-proliferative activity on human cervical (HeLa) and oral (KB) cancer cell lines of medicinal plants selected from Thai/Lanna medicinal plant recipe database "MANOSROI III". Materials and methods: Twenty-three methanolic plant crude extracts were tested for phytochemicals and anti-proliferative activity on HeLa and KB cell lines for 24h by the sulforhodamine B (SRB) assay at the doses of 1×10-1×10-6mg/ml. The nine extracts with the concentrations giving 50% growth inhibition (GI50) lower than 100μg/ml were further semi-purified by liquid/liquid partition in order to evaluate and enhance the anti-proliferative potency. Results: All extracts contained steroids/triterpenoids, but not xanthones. The methanolic extracts of Gloriosa superba L. (Colchinaceae) root and Albizia chinensis (Osbeck) Merr. (Leguminosae-Mimosoideae) wood gave the highest anti-proliferative activity on HeLa and KB cell lines with the GI50 values of 0.91 (6.0-and 0.31-fold of cisplatin and doxorubicin) and 0.16μg/ml (28.78-and 82.29-fold of cisplatin and doxorubicin), respectively. Hexane and methanol-water fractions of G. superba exhibited the highest anti-proliferative activity on HeLa and KB cell lines with the GI50 values of 0.15 (37-and 1.9-fold of cisplatin and doxorubicin) and 0.058μg/ml (77.45-and 221.46-fold of cisplatin and doxorubicin), respectively. Discussion and conclusion: This study has demonstrated the potential of plants selected from MANOSROI III database especially G. superba and A. chinensis for further development as anti-oral and cervical cancer agents. © 2015 Informa Healthcare USA, Inc. Source


Manosroi A.,Chiang Mai University | Manosroi A.,Manose Health and Beauty Research Center | Pattamapun K.,Chiang Mai University | Khositsuntiwong N.,Chiang Mai University | And 6 more authors.
Pharmaceutical Biology | Year: 2015

Context: Plant mucilages can be found in various parts of several Thai plants, which can be used as thickening, moisturizing, and lubricating agents in artificial saliva formulations. Objective: The objective of this study was to evaluate the physicochemical properties, biological activity, and cytotoxicity of Thai plant mucilages. Materials and methods: The mucilages from Thai plants were extracted by various processes (temperature and pH variation, microwave oven, steam, and Tris-HCl buffer extraction). The viscosity and the rheology were evaluated using viscometer. Antioxidative activities including DPPH radical scavenging and metal chelating activities were investigated. The mucilages were determined for cytotoxicity on normal human gingival fibroblasts and anti-adherent activity of Streptococcus mutans. Results: Mucilages from Ocimum citriodorum Vis. (Lamiaceae), Artocarpus heterophyllus Lam. (Moraceae), Abelmoschus esculentus (Linn.) Moench. (Malvaceae), and Basella alba Linn. (Basellaceae) exhibited pseudoplastic non-Newtonian rheology. The highest DPPH radicalscavenging and metal-chelating activities were observed in the mucilages from B. alba (microwave, 3 min) and A. esculentus (microwave, 1 min) with the SC50 and MC50 values (50% of scavenging activity and 50% of metal chelating activity, respectively) of 0.71 ± 0.32 and 1.11 ± 0.52 mg/ml, respectively. Most mucilages exhibited no cytotoxicity to normal human gingival fibroblasts. The mucilage from A. esculentus (microwave, 5 min) gave the shortest wetting time of 2.75 ± 0.51 min. The highest S. mutans adhesion inhibition was observed in A. esculentus (pH 11) of 5.39 ± 9.70%. Discussion and conclusion: This study has indicated the suitable physicochemical and biological properties and the potential application of mucilages from Thai plants for artificial saliva preparation. © 2015 Informa Healthcare USA, Inc. Source


Manosroi J.,Manose Health and Beauty Research Center | Manosroi J.,Chiang Mai University | Chankhampan C.,Manose Health and Beauty Research Center | Foe K.,Widya Mandala Surabaya Catholic University | And 4 more authors.
Chiang Mai Journal of Science | Year: 2016

This study aimed to increase the solubility of indomethacin in water by complexation it with hydroxypropyl-β-cyclodextrin (HPβCD). Phase-solubility analysis was used to investigate interactions in aqueous solution between HPβCD and indomethacin. Equimolar indomethacin-HPβCD solid systems were prepared by four different methods including physical mixtures (PM), kneading (KN), coevaporation (COE) and freeze-drying (COL) methods. The complex was characterized by infrared spectroscopy, differential scanning calorimetry, X-ray diffractometry, thin layer chromatography and dissolution rates. The complexation efficiency of indomethacin-HPβCD was determined spectrophotometrically. The solubility of indomethacin increased linearly as the concentration of HPβCD increased. This indicated a feature of the AL-type complex that the water-soluble complexes existed in the solution. The average of apparent 1:1 stability constant of the complex (K1:1) at 30°C was 340 M-1. The KN and COE methods formed partial inclusion complexes, whereas the COL method gave complete complexation. The dissolution rates of indomethacin increased when complexed with HPβCD. HPβCD complexation of an ionized drug molecule by the COL method exhibited the highest dissolution rate of indomethacin [the dissolution efficiency after 90 min (DE90) at 61.7±0.9% and t50% of 13 min., while the uncomplexed indomethacin showed DE90 at 15.4±0.1% and t50% more than 90 min. The COL process was the best method because of the high content and dissolution rate of the drug. It is also the simple method to prepare the inclusion complexes. The result from this study has suggested the dissolution rate enhancement of indomethacin by the simple complexaion method with HPβCD. © 2016, Chiang Mai Journal of Science. All rights reserved. Source

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