Mannheim, Germany

The Mannheim University of Applied science is a public university located in Mannheim, Germany. Commonly referred to as Hochschule Mannheim and previously known as FH Mannheim, it offers 33 degree programs at Bachelor's and Master's level in the fields of engineering, informatics, biotechnology, design, and social affairs. In German university rankings, it is usually ranked at the top-tier in view of teaching quality as well as employability of its graduates. The Faculty of Process Engineering and Chemical Engineering was placed 1st in Germany by the 2007 CHE Ranking. Wikipedia.


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Pedrolli D.B.,Mannheim University of Applied Sciences
Methods in molecular biology (Clifton, N.J.) | Year: 2014

Roseoflavin is a toxic riboflavin (vitamin B2) analog and naturally is produced by Streptomyces davawensis. Roseoflavin is converted to roseoflavin mononucleotide (RoFMN) by promiscuous flavokinases (EC 2.7.1.26). Flavin mononucleotide (FMN) riboswitches control the expression of genes involved in riboflavin biosynthesis and/or transport. RoFMN triggers FMN riboswitches and negatively (or positively) affects expression of the downstream genes. RoFMN binding to the aptamer portion of FMN riboswitch RNAs occurs in the course of transcription by cellular RNA polymerases. We developed an in vitro test system to functionally characterize the interaction between riboflavin/FMN analogs such as roseoflavin/RoFMN and FMN riboswitches in the context of an actively transcribing RNA polymerase.


Kaatze U.,University of Gottingen | Hubner C.,Mannheim University of Applied Sciences
Measurement Science and Technology | Year: 2010

Based on a short introduction to the dielectric properties of water and aqueous solutions, this review summarizes current techniques of electromagnetic aquametry. The techniques described here include time-domain and frequency-domain procedures, enabling moisture determination in a broad spectral range from millihertz to terahertz frequencies. In this review, quasi-static capacitance and impedance approaches as well as microwave and submillimetre wave reflection, transmission and resonant methods are presented. Sensor configurations and electronic measurement setups, including remote sensing, are discussed briefly. A broad spectrum of applications is introduced to show the extended usability of electromagnetic techniques of moisture content determination. The dielectric properties of some reference materials, suitable for the calibration of sensors and of electronic measurement devices, are tabulated. Reference is also made to some dielectric models of water-containing materials and to superior evaluation routines. © 2010 IOP Publishing Ltd.


Munteanu B.,Mannheim University of Applied Sciences | Hopf C.,Mannheim University of Applied Sciences
Bioanalysis | Year: 2013

Since their inception in the 1970s, methods for classification of microorganisms based on mass spectral fingerprints obtained by MALDI-TOF MS have become a mainstay in environmental as well as in clinical microbiology. Recently, related whole-cell MALDI-TOF fingerprinting workflows have been adopted for the classification of mammalian cells. In this report we summarize this work and discuss the challenges of adapting whole-cell MS fingerprinting methods for the successful classification of mammalian cells. We highlight current limitations as well as opportunities and emerging applications of this technology in industrial and clinical settings, such as cell-line authentication, clinical diagnostics, and quality and productivity control in bioprocesses. © 2013 Future Science Ltd.


Rabionet M.,German Cancer Research Center | Gorgas K.,University of Heidelberg | Sandhoff R.,German Cancer Research Center | Sandhoff R.,Mannheim University of Applied Sciences
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids | Year: 2014

The epidermis and in particular its outermost layer the stratum corneum provides terrestrial vertebrates with a pivotal defensive barrier against water loss, xenobiotics and harmful pathogens. A vital demand for this epidermal permeability barrier is the lipid-enriched lamellar matrix that embeds the enucleated corneocytes. Ceramides are the major components of these highly ordered intercellular lamellar structures, in which linoleic acid- and protein-esterified ceramides are crucial for structuring and maintaining skin barrier integrity. In this review, we describe the fascinating diversity of epidermal ceramides including 1-O-acylceramides. We focus on epidermal ceramide biosynthesis emphasizing its metabolic and topological requirements and discuss enzymes that may be involved in α- and ω-hydroxylation. Finally, we turn to epidermal ceramide regulation, highlighting transcription factors and liposensors recently described to play crucial roles in modulating skin lipid metabolism and epidermal barrier homeostasis. This article is part of a Special Issue entitled The Important Role of Lipids in the Epidermis and their Role in the Formation and Maintenance of the Cutaneous Barrier. © 2013 Elsevier B.V.


Fredericks G.J.,University of Hawaii at Manoa | Hoffmann F.W.,University of Hawaii at Manoa | Rose A.H.,University of Hawaii at Manoa | Osterheld H.J.,Mannheim University of Applied Sciences | And 3 more authors.
Proceedings of the National Academy of Sciences of the United States of America | Year: 2014

Calcium (Ca2+) is a secondary messenger in cells and Ca2+ flux initiated from endoplasmic reticulum (ER) stores via inositol 1,4,5-triphosphate (IP3) binding to the IP3 receptor (IP3R) is particularly important for the activation and function of immune cells. Previous studies demonstrated that genetic deletion of selenoprotein K (Selk) led to decreased Ca2+ flux in a variety of immune cells and impaired immunity, but the mechanism was unclear. Here we show that Selk deficiency does not affect receptor-induced IP3 production, but Selk deficiency through genetic deletion or low selenium in culture media leads to low expression of the IP3R due to a defect in IP3R palmitoylation. Bioinformatic analysis of the DHHC (letters represent the amino acids aspartic acid, histidine, histidine, and cysteine in the catalytic domain) family of enzymes that catalyze protein palmitoylation revealed that one member, DHHC6, contains a predicted Src-homology 3 (SH3) domain and DHHC6 is localized to the ER membrane. Because Selk is also an ER membrane protein and contains an SH3 binding domain, immunofluorescence and coimmunoprecipitation experiments were conducted and revealed DHHC6/Selk interactions in the ER membrane that depended on SH3/SH3 binding domain interactions. DHHC6 knockdown using shRNA in stably transfected cell lines led to decreased expression of the IP3R and impaired IP3R-dependent Ca2+ flux. Mass spectrophotometric and bioinformatic analyses of the IP3R protein identified two palmitoylated cysteine residues and another potentially palmitoylated cysteine, and mutation of these three cysteines to alanines resulted in decreased IP3R palmitoylation and function. These findings reveal IP3R palmitoylation as a critical regulator of Ca2+ flux in immune cells and define a previously unidentified DHHC/Selk complex responsible for this process.


Fimmel E.,Mannheim University of Applied Sciences | Michel C.J.,University of Strasbourg | Strungmann L.,Mannheim University of Applied Sciences
Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences | Year: 2016

The circular code theory proposes that genes are constituted of two trinucleotide codes: the classical genetic code with 61 trinucleotides for coding the 20 amino acids (except the three stop codons {TAA, TAG,TGA}) and a circular code based on 20 trinucleotides for retrieving, maintaining and synchronizing the reading frame. It relies on two main results: the identification of a maximal C3 self-complementary trinucleotide circular code X in genes of bacteria, eukaryotes, plasmids and viruses (Michel 2015 J. Theor. Biol. 380, 156-177. (doi:10.1016/j.jtbi.2015.04.009); Arquès &Michel 1996 J. Theor. Biol. 182, 45-58. (doi:10.1006/jtbi.1996.0142)) and the finding of X circular code motifs in tRNAs and rRNAs, in particular in the ribosome decoding centre (Michel 2012 Comput. Biol. Chem. 37, 24-37. (doi:10.1016/j.compbiolchem.2011.10.002); El Soufi &Michel 2014 Comput. Biol. Chem. 52, 9-17. (doi:10.1016/j.compbiolchem.2014.08.001)). The univerally conserved nucleotides A1492 and A1493 and the conserved nucleotide G530 are included in X circular code motifs. Recently, dinucleotide circular codes were also investigated (Michel &Pirillo 2013 ISRN Biomath. 2013, 538631. (doi:10.1155/2013/538631); Fimmel et al. 2015 J. Theor. Biol. 386, 159-165. (doi:10.1016/j.jtbi.2015.08.034)). As the genetic motifs of different lengths are ubiquitous in genes and genomes, we introduce a new approach based on graph theory to study in full generality n-nucleotide circular codes X, i.e. of length 2 (dinucleotide), 3 (trinucleotide), 4 (tetranucleotide), etc. Indeed, we prove that an n-nucleotide code X is circular if and only if the corresponding graph G(X) is acyclic. Moreover, the maximal length of a path in G(X) corresponds to the window of nucleotides in a sequence for detecting the correct reading frame. Finally, the graph theory of tournaments is applied to the study of dinucleotide circular codes. It has full equivalence between the combinatorics theory (Michel &Pirillo 2013 ISRN Biomath. 2013, 538631. (doi:10.1155/2013/538631)) and the group theory (Fimmel et al. 2015 J. Theor. Biol. 386, 159-165. (doi:10.1016/j.jtbi.2015.08.034)) of dinucleotide circular codes while its mathematical approach is simpler. © 2016 The Author(s) Published by the Royal Society. All rights reserved.


Fimmel E.,Mannheim University of Applied Sciences | Strungmann L.,Mannheim University of Applied Sciences
Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences | Year: 2016

Yury Borisovich Rumer was one of the most important theoretical physicists of the former Soviet Union in the early 1930s. However, he also wrote a few 'biological papers' on the standard genetic code after he read Crick's and Nirenberg's pioneering papers on the topic. Rumer's articles on the 'Systematization of Codons in the Genetic Code' (Rumer 1966 Doklady Akademii nauk SSSR 167, 1393-1394); Rumer 1968 Doklady Akademii nauk SSSR 183, 225-226; Rumer 1969 Doklady Akademii nauk SSSR 187, 937-938, where he suggested the idea of partitioning codons depending on their redundancy-the first mention of symmetry in the genetic code-were published in Russian only. Due to their importance and their frequent citation, we here present translations of these articles into English in order to make them accessible to a broader community. © 2016 The Author(s) Published by the Royal Society. All rights reserved.


Pedrolli D.B.,Mannheim University of Applied Sciences | Mack M.,Mannheim University of Applied Sciences
Methods in Molecular Biology | Year: 2014

Roseoflavin is a toxic riboflavin (vitamin B2) analog and naturally is produced by Streptomyces davawensis. Roseoflavin is converted to roseoflavin mononucleotide (RoFMN) by promiscuous flavokinases (EC 2.7.1.26). Flavin mononucleotide (FMN) riboswitches control the expression of genes involved in riboflavin biosynthesis and/or transport. RoFMN triggers FMN riboswitches and negatively (or positively) affects expression of the downstream genes. RoFMN binding to the aptamer portion of FMN riboswitch RNAs occurs in the course of transcription by cellular RNA polymerases. We developed an in vitro test system to functionally characterize the interaction between riboflavin/FMN analogs such as roseoflavin/RoFMN and FMN riboswitches in the context of an actively transcribing RNA polymerase. © Springer Science+Business Media New York 2014.


Schoper Y.,Mannheim University of Applied Sciences
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) | Year: 2013

Global competition requires that new technical products are launched at the same time in all relevant global markets. Therefore the Human Computer Interface (HCI) product designers need to know all requirements of all global customer groups before starting the global development process of user interfaces (UI), which takes normally place in dispersed intercultural mixed UI designer teams. Therefore the user-centered design process from ISO 9241-210 is analyzed concerning the requirements of intercultural management and particularly of intercultural project management. On this basis, an agile intercultural HCI design management process is developed. The resulting UI design approach empowered by cultural aspects makes sure that new systems are designed right from the beginning for the cultural diverse user markets in a time and cost efficient and effective way. © 2013 Springer-Verlag Berlin Heidelberg.


Rey-Giraud F.,Hoffmann-La Roche | Rey-Giraud F.,Mannheim University of Applied Sciences | Hafner M.,Mannheim University of Applied Sciences | Ries C.H.,Hoffmann-La Roche
PLoS ONE | Year: 2012

The tumor promoting role of M2 macrophages has been described in in vivo models and the presence of macrophages in certain tumor types has been linked to a poor clinical outcome. In light of burgeoning activities to clinically develop new therapies targeting tumor-associated macrophages (TAMs), reliable in vitro models faithfully mimicking the tumor promoting functions of TAMs are required. Generation and activation of human monocyte-derived macrophages (MDM) in vitro, described as M1 or M2 macrophages attributed with tumoricidal or tumor-promoting functions, respectively, has been widely reported using mainly serum containing culture methods. In this study, we compared the properties of macrophages originating from monocytes cultured either in media containing serum together with M-CSF for M2 and GM-CSF for M1 macrophages or in serum-free media supplemented with M-CSF or GM-CSF and cytokines such as IL-4, IL-10 to induce activated M2 or LPS together with IFN-γ to generate activated M1 phenotype. We observed differences in cell morphology as well as increased surface receptor expression levels in serum-containing culture whereas similar or higher cytokine production levels were detected under serum-free culture conditions. More importantly, MDM differentiated under serum-free conditions displayed enhanced tumoricidal activity for M1 and tumor promoting property for M2 macrophages in contrast to MDM differentiated in the presence of serum. Moreover, evaluation of MDM phagocytic activity in serum free condition resulted in greater phagocytic properties of M2 compared to M1. Our data therefore confirm the tumor promoting properties of M2 macrophages in vitro and encourage the targeting of TAMs for cancer therapy. © 2012 Rey-Giraud et al.

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