The Mannheim University of Applied science is a public university located in Mannheim, Germany. Commonly referred to as Hochschule Mannheim and previously known as FH Mannheim, it offers 33 degree programs at Bachelor's and Master's level in the fields of engineering, informatics, biotechnology, design, and social affairs. In German university rankings, it is usually ranked at the top-tier in view of teaching quality as well as employability of its graduates. The Faculty of Process Engineering and Chemical Engineering was placed 1st in Germany by the 2007 CHE Ranking. Wikipedia.
Kaatze U.,University of Gottingen |
Hubner C.,Mannheim University of Applied Sciences
Measurement Science and Technology | Year: 2010
Based on a short introduction to the dielectric properties of water and aqueous solutions, this review summarizes current techniques of electromagnetic aquametry. The techniques described here include time-domain and frequency-domain procedures, enabling moisture determination in a broad spectral range from millihertz to terahertz frequencies. In this review, quasi-static capacitance and impedance approaches as well as microwave and submillimetre wave reflection, transmission and resonant methods are presented. Sensor configurations and electronic measurement setups, including remote sensing, are discussed briefly. A broad spectrum of applications is introduced to show the extended usability of electromagnetic techniques of moisture content determination. The dielectric properties of some reference materials, suitable for the calibration of sensors and of electronic measurement devices, are tabulated. Reference is also made to some dielectric models of water-containing materials and to superior evaluation routines. © 2010 IOP Publishing Ltd.
Fredericks G.J.,University of Hawaii at Manoa |
Hoffmann F.W.,University of Hawaii at Manoa |
Rose A.H.,University of Hawaii at Manoa |
Osterheld H.J.,Mannheim University of Applied Sciences |
And 3 more authors.
Proceedings of the National Academy of Sciences of the United States of America | Year: 2014
Calcium (Ca2+) is a secondary messenger in cells and Ca2+ flux initiated from endoplasmic reticulum (ER) stores via inositol 1,4,5-triphosphate (IP3) binding to the IP3 receptor (IP3R) is particularly important for the activation and function of immune cells. Previous studies demonstrated that genetic deletion of selenoprotein K (Selk) led to decreased Ca2+ flux in a variety of immune cells and impaired immunity, but the mechanism was unclear. Here we show that Selk deficiency does not affect receptor-induced IP3 production, but Selk deficiency through genetic deletion or low selenium in culture media leads to low expression of the IP3R due to a defect in IP3R palmitoylation. Bioinformatic analysis of the DHHC (letters represent the amino acids aspartic acid, histidine, histidine, and cysteine in the catalytic domain) family of enzymes that catalyze protein palmitoylation revealed that one member, DHHC6, contains a predicted Src-homology 3 (SH3) domain and DHHC6 is localized to the ER membrane. Because Selk is also an ER membrane protein and contains an SH3 binding domain, immunofluorescence and coimmunoprecipitation experiments were conducted and revealed DHHC6/Selk interactions in the ER membrane that depended on SH3/SH3 binding domain interactions. DHHC6 knockdown using shRNA in stably transfected cell lines led to decreased expression of the IP3R and impaired IP3R-dependent Ca2+ flux. Mass spectrophotometric and bioinformatic analyses of the IP3R protein identified two palmitoylated cysteine residues and another potentially palmitoylated cysteine, and mutation of these three cysteines to alanines resulted in decreased IP3R palmitoylation and function. These findings reveal IP3R palmitoylation as a critical regulator of Ca2+ flux in immune cells and define a previously unidentified DHHC/Selk complex responsible for this process.
Rabionet M.,German Cancer Research Center |
Gorgas K.,University of Heidelberg |
Sandhoff R.,German Cancer Research Center |
Sandhoff R.,Mannheim University of Applied Sciences
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids | Year: 2014
The epidermis and in particular its outermost layer the stratum corneum provides terrestrial vertebrates with a pivotal defensive barrier against water loss, xenobiotics and harmful pathogens. A vital demand for this epidermal permeability barrier is the lipid-enriched lamellar matrix that embeds the enucleated corneocytes. Ceramides are the major components of these highly ordered intercellular lamellar structures, in which linoleic acid- and protein-esterified ceramides are crucial for structuring and maintaining skin barrier integrity. In this review, we describe the fascinating diversity of epidermal ceramides including 1-O-acylceramides. We focus on epidermal ceramide biosynthesis emphasizing its metabolic and topological requirements and discuss enzymes that may be involved in α- and ω-hydroxylation. Finally, we turn to epidermal ceramide regulation, highlighting transcription factors and liposensors recently described to play crucial roles in modulating skin lipid metabolism and epidermal barrier homeostasis. This article is part of a Special Issue entitled The Important Role of Lipids in the Epidermis and their Role in the Formation and Maintenance of the Cutaneous Barrier. © 2013 Elsevier B.V.
Pedrolli D.B.,Mannheim University of Applied Sciences
Methods in molecular biology (Clifton, N.J.) | Year: 2014
Roseoflavin is a toxic riboflavin (vitamin B2) analog and naturally is produced by Streptomyces davawensis. Roseoflavin is converted to roseoflavin mononucleotide (RoFMN) by promiscuous flavokinases (EC 126.96.36.199). Flavin mononucleotide (FMN) riboswitches control the expression of genes involved in riboflavin biosynthesis and/or transport. RoFMN triggers FMN riboswitches and negatively (or positively) affects expression of the downstream genes. RoFMN binding to the aptamer portion of FMN riboswitch RNAs occurs in the course of transcription by cellular RNA polymerases. We developed an in vitro test system to functionally characterize the interaction between riboflavin/FMN analogs such as roseoflavin/RoFMN and FMN riboswitches in the context of an actively transcribing RNA polymerase.
Munteanu B.,Mannheim University of Applied Sciences |
Hopf C.,Mannheim University of Applied Sciences
Bioanalysis | Year: 2013
Since their inception in the 1970s, methods for classification of microorganisms based on mass spectral fingerprints obtained by MALDI-TOF MS have become a mainstay in environmental as well as in clinical microbiology. Recently, related whole-cell MALDI-TOF fingerprinting workflows have been adopted for the classification of mammalian cells. In this report we summarize this work and discuss the challenges of adapting whole-cell MS fingerprinting methods for the successful classification of mammalian cells. We highlight current limitations as well as opportunities and emerging applications of this technology in industrial and clinical settings, such as cell-line authentication, clinical diagnostics, and quality and productivity control in bioprocesses. © 2013 Future Science Ltd.