Characterization of autochthonous grapevine cultivars (Vitis vinifera L.) from the Aegean region of Turkey using simple sequence repeats (SSRs) [Ege bölgesi yerel asma çeşitlerinin (Vitis vinifera L.) basit tekrar dizileri (SSRs) ile karakterizasyonu]
Isci B.,Ege University |
Dilli Y.,Manisa Viticulture Research Station
Tarim Bilimleri Dergisi | Year: 2015
Thirty-six autochthonous grapevine cultivars from the Aegean region of Turkey in addition to standard cultivars Cabernet Sauvignon and Merlot (Vitis vinifera L.) were fingerprinted using SSR markers to assess their genetic relationships. Eleven SSR primers produced successful amplifications and yielded 37 polymorphic bands. The number of bands per primer changed between 2 and 6 while the number of polymorphic bands was between 2 and 3.6. Dice genetic similarity coefficients ranged between 0.296 and 0.882 among the genotypes. The UPGMA dendrogram showed two main groups. The first group was composed of Sultan Dimriti, Veyis and Güvercin Gözü cultivars. A large number of sub-groups were placed in the second group which included the majority of autochthonous cultivars. The genetic differences among the autochthonous cultivars and reference cultivars were clearly observed. The results showed that cultivars Siyah Razakı and Parmak (0.96) were the most similar ones. Synonyms were identified for İnek Memesi and Ufak Dimrit cultivars. In addition, homonymous cultivars were detected. SSR markers have proved to be an efficient tool for fingerprinting grapevine cultivars and conducting genetic diversity studies. © Ankara Üniversitesi Ziraat Fakültesi.
Sen F.,Ege University |
Kesgin M.,Manisa Viticulture Research Station
Food Science and Technology | Year: 2015
Covering the grapevine rows to delay the maturity and harvest date became widely practiced in ‘Sultana Seedless’ vineyards. The research work was conducted to test different cover materials (polypropylene cross-stitch, life pack, mogul and transparent polyethylene) in respect to their effects on grape quality and storability. Harvest was delayed for one month in covered plots. Harvested grapes were packed and transferred to storage rooms after pre-cooling. During packing, the grape clusters were sealed in PE bags with sulphur dioxide pads. The grapes were stored for 90 days in the first year and 120 days in the second year, at –0.5°C and 90% RH. All the grape clusters were healthy and of marketable quality after 90 days of storage period. In the first year, at the end of the storage, only those grapes harvested from the rows covered with polypropylene cross-stitch showed fungal growth. The sensory quality scores revealed a lower level of preference after 120 days of storage. The effects of the covering materials tested were similar regarding grape quality and storage performance except the transparent polyethylene that damaged the grapevine leaves. © 2014, Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA. All rights reserved.
Paylan I.C.,Ege University |
Candar A.,Ege University |
Gumus M.,Ege University |
Pazarlar S.,Ege University |
Onder S.,Manisa Viticulture Research Station
Pakistan Journal of Botany | Year: 2014
Isolation of high quality RNA from plant tissues is one of the most critical steps for the successful application of diagnostic tests such as reverse transcription polymerase chain reaction (RT-PCR), northern blotting, microarray hybridization. The presence of inhibitors such as secondary metabolites, phenolic compounds and RNAses can cause inaccurate and undesirable results. Grapevine is rich in a wide range of metabolites which interfere with RNA isolation. From this point of view, we researched six different total RNA extraction methods on leaves of Vitisvinifera L. to find the best one that contribute the purity and high quality. The methods tested are silica-capture, modified silica-capture, commercial kit, the new combined, lithium chloride and citric buffer. RNA quality was analyzedspectrophotometrically by nanodrop, agarose gel electrophoresis and RT-PCR. As a result of all, it is clear that the most suitable TNA isolation protocol is the new combined method which experienced and named firstly by us, in terms of RNA purity, concentration, less time consuming of isolation step and achievement on detection of GYSVd-1. © 2014, Pakistan Botanical Society. All rights reserved.
Sen F.,Ege University |
Oksar R.E.,Ege University |
Kesgin M.,Manisa Viticulture Research Station
Journal of Agricultural Science and Technology | Year: 2016
This study aimed to determine the effects of different shading ratio and covers on“Sultana Seedless” table grape quality and storability. Grapevines were covered at theveraison stage with 3 shading nets (0, 35, and 75% shading), which were then replacedwith covering materials including Polypropylene Cross-stitch (PC), Life Pack (LP) andMogul (MG), just before harvest. Harvested grapes in all treatments were then kept for90 days under cold storage (-05°C). Postharvest decay development and decreases insensory quality were observed in unshaded grapes and in those covered with 35% shadingnet and later covered with life pack. Total soluble solids content, maturity index, andcolor value (C* and h°either decreased or vanishedduring storage. All tested cover materials following shading nets could delay harvest for50 days. Consequently, in addition to delaying harvest time, PC or MG covers used after35 % shading allowed higher quality and successful storage of grapes for an additional 90 days. © 2016, Tarbiat Modares University. All rights reserved.
Savas N.G.,Manisa Viticulture Research Station |
Akgul D.S.,Cukurova University |
Albaz E.A.,Manisa Viticulture Research Station
Plant Disease | Year: 2015
Grapevine trunk diseases have been an increasing problem of Turkey vineyards in recent years. A study was conducted in nine Chardonnay (grafted on 110R) vineyards to determine the incidence and the causal agents of grapevine trunk diseases in Sarkoy, Tekirdag (northwest of Turkey) in 2014. The vines with tiger-striped leaves, and declining, local drying, or dead arm symptoms (27.3% of all inspected 23291 vines) were recorded and branch, trunk, and rootstock samples (three samples from each vineyard) were taken for laboratory examinations. Dark brown and black spots in cross sections and blackish streaks in longitudinal sections were observed in wood of some rootstocks. Symptomatic tissues were surface disinfested with 95% ethanol and flame sterilized. The internal tissues were plated onto potato dextrose agar amended with tetracycline (0.01%). Petri dishes were incubated at 24°C in the dark for 18 days and floccose to felted colonies, which varied in color from dark orange to dark brown with age, developed (in one vineyard, with 24.3% isolation frequency) around wood chips. Pure cultures of the isolate (MBAi232) produced 1 to 3-septate, straight or slightly curved, cylindrical macroconidia (32 to 38 × 4.9 to 5.7 µm) and a few microconidia. Chlamydospores were brown, ovoid (12 to 17 × 10 to 17 μm), and mostly in short and intercalary in chains. According to morphological characteristics, the isolate resembled Ilyonectria liriodendri with species nomenclature based on the work of Halleen et al. 2006. For molecular identification, fungal DNA was extracted from mycelium and ribosomal DNA fragments (ITS1, 5.8S ITS2 rDNA) and β-tubulin gene were amplified with ITS 4-5, Bt 2a-2b primers (White 1990; Glass and Donaldson 1995) and sequenced. The sequences were compared with those deposited in GenBank and the isolate showed 99% similarity with I. liriodendri isolates JF735261 (ITS) and KF511973 (β-tubulin). The sequences were deposited as NCBI GenBank Accession Nos. KP271987, KP271986 for ITS, and β-tubulin, respectively. Pathogenicity tests were conducted under greenhouse conditions (24°C, 16/8h day/night, 70% RH) on own-rooted grapevine (Vitis vinifera) seedlings cv. Chardonnay using the isolate of I. liriodendri. Plants were removed from the rooting bench, and the roots were slightly trimmed and dip-inoculated with a 106/ml conidial suspension of the isolate for 60 min (Cabral et al. 2012). After inoculation, the rooted cuttings were planted in 1-liter bags containing a mixture of soil, peat, and sand (2:1:1, v/v/v), and maintained in the greenhouse. Sterile distilled water was used for inoculation of negative control plants. Ten vines were inoculated with the isolate and 5 vines were not inoculated. The experiment was repeated twice. After 4 months of incubation, seedlings were examined for vascular discoloration and recovery of fungal isolate. Blackish-brown discoloration of xylem vessels and necrosis in the basal ends and roots were visible in inoculated but not in uninoculated control seedlings. Slight leaf wilting was also observed in some plants. The fungal isolate was successfully reisolated (by 70.5%) from inoculated but not from uninoculated ones. To our knowledge, this is the first report of I. liriodendri causing black-foot disease of grapevine in Turkey. © 2015 The American Phytopathological Society.