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Manipala, India

Mudiganty S.,Manipal UniversityKarnataka | Sipani A.K.,Silchar Medical College and Hospital | Das S.K.,Tezpur Medical College and Hospital
JMS - Journal of Medical Society | Year: 2015

A 13-year-old female presented with chronic instability of her left hip due to late sequela of infantile hip infection. Instability of hip causes significant problems due to pain, limp, and shortening. Such a case is rare and difficult to treat. We performed a subtrochanteric valgus extension pelvic support osteotomy, along with distal varization and lengthening osteotomy utilizing a monolateral fixator. The patient was clinically and radiologically followed up for 24 months after her operation. The fixator was removed after 12 months once radiological union and adequate lengthening was achieved. Pre- and postoperative assessments were done using the Harris Hip Score, which showed a significant improvement. Long duration of usage of the fixator, knee stiffness, and pin tract infections are a few of the limitations observed. © 2015 Journal of Medical Society.

Kaur H.,Regional Center for Biotechnology | Kaur H.,Manipal UniversityKarnataka | Salunke D.M.,Regional Center for Biotechnology | Salunke D.M.,National Institute of Immunology
IUBMB Life | Year: 2015

Affinity maturation is associated with reduced malleability of the paratope that optimizes an antibody to bind to the bonafide antigen with high specificity and affinity. However, it has been illustrated that mature antibodies tend to exhibit promiscuity despite acquisition of a relatively rigid binding pocket. Such an attribute is contrary to the established paradigm of specificity in antigen recognition. In this review, an explicit dissection of the underlying mechanisms fostering such versatility in mature antibodies has been done. Polyspecificity is essentially achieved by undergoing minimal structural rearrangement at the paratope complemented with plasticity in interaction with antigen. Besides, the structural invariance of the antigen across species could modulate mature antibody specificity. Polyreactivity has been well documented for germline antibodies as broad spectrum antibody repertoire amplification is primarily governed by recombination event of the genetic machinery, which is further expanded at the structural and functional level of interaction. Degenerate specificity in antigen recognition obviates the need to produce distinct antibody for every incoming epitope © 2015 IUBMB Life, 67(7):498-505, 2015 © 2015 International Union of Biochemistry and Molecular Biology.

Samantara A.K.,CSIR - Institute of Minerals And Materials Technology | Samantara A.K.,Academy of Scientific and Innovative Research AcSIR | Maji S.,Institute of Life science | Maji S.,Manipal UniversityKarnataka | And 6 more authors.
Journal of Materials Chemistry B | Year: 2016

Here, a facile one-step approach has been developed for the synthesis of carbon quantum dots (CQDs) from Good's buffer. The as-synthesized CQDs emit a bright greenish blue coloured fluorescence after exposure to a 365 nm UV-lamp illumination. The bright fluorescence nature of the CQDs has proven them to be excellent probes for cellular imaging. The CQDs are highly biocompatible in nature, which has been validated by an MTT assay test. The in vitro MTT assay demonstrates a more than 95% survival rate when HEK293 (human embryonic kidney) and H357 (human oral squamous carcinoma) cells were treated with CQDs. The low cytotoxicity of Good's buffer derived CQDs opens the door to biomedical applications. The anticancer drug doxorubicin (DOX) was successfully loaded on the CQDs and their delivery efficiency to the target cells via in vitro treatment of cancerous cells was explored. The CQDs supported DOX showed a higher killing rate of the cancer cells compared to bare DOX due to its ease of internalization and efficient pH-triggered release inside the cells. © The Royal Society of Chemistry 2016.

Amit-Byatnal A.,Manipal UniversityKarnataka | Natarajan J.,Manipal UniversityKarnataka | Shenoy S.,Manipal University India | Kamath A.,Manipal UniversityKarnataka | And 3 more authors.
Medicina Oral, Patologia Oral y Cirugia Bucal | Year: 2015

Background: Accurate assessment of the depth of tumor invasion (DI) in microinvasive squamous cell carcinoma (MISCC) of the tongue is critical to prognosis. An arithmetic model is generated to determine a reliable method of measurement of DI and correlate this with the local recurrence. Material and Methods: Tumor thickness (TT) and DI were measured in tissue sections of 14 cases of MISCC of the tongue, by manual ocular micrometer and digital image analysis at four reference points (A, B, C, and D). The comparison of TT and DI with relevant clinicopathologic parameters was assessed using Mann Whitney U test. Reliability of these methods and the values obtained were compared and correlated with the recurrence of tumors by Wilcoxon Signed Ranks Test. 3D reconstruction of the lesion was done on a Cartesian coordinate system. X face was on the YZ plane and Z face was on the XY plane of the coordinate system. Results: Computer generated 3D model of oral mucosa in four cases that recurred showed increased DI in the Z coordinate compared to the XY coordinate. The median DI measurements between XY and Z coordinates in these cases showed no significant difference (Wilcoxon Signed Ranks Test, p = 0.068). Conclusions: The assessment of DI in 3 dimensions is critical for accurate assessment of MISCC and precise DI allows complete removal of tumor. © Medicina Oral S. L.

Shetty J.K.,United Arab Emirates University | Maradi R.,Manipal UniversityKarnataka | Prabhu K.,Manipal UniversityKarnataka | Bhat G.,Manipal UniversityKarnataka
Journal of Clinical and Diagnostic Research | Year: 2015

Bisalbuminemia is a rarely encountered anomaly characterized by presence of bifid albumin bands or a single widened albumin band in electrophoretogram. Inherited bisalbuminemia is quite rare and inherited as an autosomal dominant form. The acquired form of bisalbuminemia is usually transient and may be observed during long term beta lactam antibiotic therapy, acute pancreatitis, myeloma and nephrotic syndrome. This is a case of bisalbuminemia in 61-year-old diabetic female with hypothyroidism came with acute exacerbation of bronchial asthma. © 2015,Journal of Clinical and Diagnostic Research. All rights reserved.

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