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Manipala, India

Kumar D.,Manipal University India | Upadhya D.,Manipal University India | Salian S.R.,Manipal University India | Rao S.B.S.,Manipal Life science Center | And 3 more authors.
European Journal of Obstetrics Gynecology and Reproductive Biology

Objectives: To study the post-natal characteristics and the survival of offspring derived from DNA damaged sperm. Study design: This experimental prospective study was conducted on Swiss Albino mice (Mus musculus). Sperm DNA damage was induced by different doses of γ-irradiation in male mice who were then mated with healthy female mice. The post-natal characteristics including the survival of first generation offspring were studied and then correlated with the amount of paternal sperm DNA damage. Results: A significant reduction of survival in the early post-natal period was observed in the first generation offspring derived from the DNA damaged sperm, and a strong association was observed between the extent of sperm DNA damage and the survival of the offspring. Conclusion: The DNA damage load in sperm at the time of fertilization influences early post-natal survival of the mouse offspring. © 2012 Elsevier Ireland Ltd. Source

Ghumman S.,Manipal University India | Adiga S.K.,Manipal University India | Upadhya D.,Manipal University India | Kalthur G.,Manipal University India | And 3 more authors.
Journal of the Turkish German Gynecology Association

Objective: The aim of this experimental prospective study was to investigate the efficacy of single and combination sperm wash methods for their ability to isolate DNA intact spermatozoa. Material and Methods: Sperm DNA damage was introduced by local testicular irradiation in male mice and the extent of damage was quantified by comet assay. The spermatozoa were subjected to single (swim up or density gradient method) and also a combination of sperm wash techniques. The DNA integrity in various sub-fractions of wash techniques was evaluated. Results: The amount of DNA damaged sperm did not differ between individual fractions when single wash technique was applied. However, a combination of density gradient and swim-up techniques significantly reduced (p<0.01) the number of DNA damaged sperm in the final population. Conclusion: The combination of density gradient separation and swim-up method is effective in eliminating DNA damaged spermatozoa. © 2011 by the Turkish-German Gynecological Education and Research Foundation. Source

Rao U.S.C.,Jawaharlal Institute of Postgraduate Medical Education & Research | Devendran A.,Jawaharlal Institute of Postgraduate Medical Education & Research | Satyamoorthy K.,Manipal Life science Center | Shewade D.G.,Jawaharlal Institute of Postgraduate Medical Education & Research | And 2 more authors.
Molecular Biology Reports

CYP2C19 is an enzyme involved in the metabolism of several clinically important drugs. The variations in the CYP2C19 promoter region may alter the transcription of the gene by altering the interaction between the trans and cis-acting elements. In the present study, CYP2C19 promoter region with different variant alleles were cloned into a pGL-3 basic luciferase reporter vector and transfected into HepG2 cell lines. Subsequently, dual luciferase activity was measured to evaluate the activity of the promoter region. Gel shift assays with predicted binding sites for CCAAT displacement protein, activating transcription factor-2 and glucocorticoid receptor were performed. Results from this study indicate that few variations present in the putative cis-acting elements of the CYP2C19 promoter region such as -1442T>C, -779A>C and -98T>C -1498T>G and -828del>T alter the transcription of the gene. Specific binding with nuclear proteins was also observed in gel shift assays. This may account for the interindividual variations in gene expression and genotype dependant differences in gene transcription. The results also suggest the role of activating transcription factor-2 and CCAAT displacement repressor protein on CYP2C19 gene transcription. © Springer Science+Business Media B.V. 2010. Source

Adiga S.K.,Manipal University India | Upadhya D.,Manipal University India | Kalthur G.,Manipal University India | Bola Sadashiva S.R.,Manipal Life science Center | Kumar P.,Manipal University India
Fertility and Sterility

Objective: To report a more quantitative approach to study the influence of varying levels of sperm DNA damage on transgenerational changes in genomic instability in a mouse model. Design: Experimental prospective study. Setting: Embryology research laboratory. Animal(s): Swiss albino mice. Intervention(s): The sperm DNA damage was induced by different doses of γ-irradiation to male mice followed by mating with healthy female mice. Main Outcome Measure(s): Genomic integrity in embryos, fetus, and spermatozoa of F1 mice derived from the DNA-damaged sperm. Result(s): The transgenerational changes in genetic integrity were attributed by a dose-dependent increase in the frequency of micronuclei in preimplantation embryos and a concomitant increase in genomic instability in fetal liver cells and sperm chromatin modifications in F1 males. A strong positive correlation was observed between the extent of sperm DNA damage and somatic and germ-line genomic instability. Conclusion(s): Sperm-mediated transgenerational genomic instability is dependent on the amount of DNA damage present in the sire's sperm at the time of fertilization. © 2010 American Society for Reproductive Medicine. Source

Kodandapani S.,Kasturba Medical College | Pai M.V.,Kasturba Medical College | Nambiar J.,Kasturba Medical College | Moka R.,Manipal Life science Center
Journal of Human Reproductive Sciences

Genetic aberrations comprise one-third of women with premature ovarian aging (POA). X chromosome abnormalities are seen in these women. We report a case of a 29-year-old lady with primary infertility and POA. She was phenotypically normal and her basal follicle stimulating hormone level was above the age-specific cut-off. Karyotype was triple X syndrome. Source

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