Dey S.K.,Khulna University |
Hira A.,Khulna University |
Ahmed A.,Khulna University |
Howlader S.I.,World University of Bangladesh |
And 3 more authors.
International Journal of Applied Research in Natural Products | Year: 2013
Entada scandens (E. scandens) (family. Mimosaceae) is a widely used medicinal plant has been traditionally used by the folklore medicinal practitioners of Bangladesh to treat pain, cancer, gastrointestinal disorders where antinociceptive, cytotoxic and anti-diarrheal medications are implicated. Therefore, phytochemical groups and antinociceptive, cytotoxic, and anti-diarrheal activities of ethanol extract of seed of E. scandens were investigated by using acetic acid induced writhing model in mice, brine shrimp lethality bioassay and castor oil induced diarrheal model in mice. Phytochemical study of the extract indicated the presence of alkaloids, glycosides, tannins, flavonoids and saponins. At the doses of 250 and 500 mg/kg body weight, the extract showed a significant antinociceptive activity showing 60.61 and 72.73% inhibition respectively (P<0.001) comparable to that produced by Diclofenac Na (80.30%) used as standard drug. The extract showed significant toxicity in the brine shrimp lethality bioassay (LC50. 20μg/ml & LC90. 80μg/ml). While evaluating anti-diarrheal activity, the extract inhibited the mean number of defecation which were 13.21% (P<0.01) and 22.64 % (P<0.001) at the doses of 250 and 500mg/kg respectively. The latent period for the extract treated group was (p<0.01) increased as compared to control group. In addition, antimicrobial study was carried out by disc diffusion assay, but no significant inhibition was found against Escherichia coli, Pseudomonas aureus, Plesiomonas shigelloides, Salmonella typhi, S. paratyphi, Shigella dysenteriae, S. flexneri, S. boydii, S. sonnei, Proteus vulgaris, Enterococcus faecalis, Staphylococcus saprophyticus, S. aureus, S. epidermidis and Streptococcus pyogenes. The study tends to suggest the antinociceptive, cytotoxic and antidiarrheal activities of the crude ethanol extract of the seed of E. scandens and justify its use in folkloric remedies. Industrial relevance. Medicinal plants can form an excellent source for derivation of lead compounds or newer drugs. The knowledge base of folk medicinal practitioners can in this instance form an invaluable source on which further scientific studies may be based, for the folk medicinal practices of the Kavirajes date back to centuries ago. They also have proved to be a rich source of new active compounds which are less toxic and less costly when compared to the synthetic drugs. The present study will help the industry to produce herbal drug with less side effect, economically affordable and more effective in the treatment of pain, diarrhea and inflammation processes. Finally the phytochemical screening from the plant would be effective drug for the antinociceptive, cytotoxic and antidiarrheal activities of the crude ethanol extract of the seed of E. scandens. © 2008-2013. IJARNP-HS Publication.
Zahan R.,Atish Dipankar University of Science and Technology |
Badrul Alam M.,Atish Dipankar University of Science and Technology |
Saiful Islam M.,International Islamic University Chittagong |
Sarker G.C.,Square Pharmaceuticals Ltd |
And 5 more authors.
International Journal of Cancer Research | Year: 2011
The research study was conducted to determine the antitumor effect of the flower of Alangium salvifolium (crude extract and diethylether fractions) against Ehrlich Ascites Carcinoma (EAC) in mice at the doses of 10 mg kg-1 body weight intraperitoneally. Extract/fractions was administered for nine consecutive days. Twenty-four hours of last dose and 18 h of fasting, the mice were sacrificed and antitumor effect was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight and hematological parameters of EAC bearing host. Significant (p<0.001) increases of survival times 30±0.96 and 25±0.40 days for crude extract and diethylether fraction of the A. salvifolium (10 mg kg-1) treated tumor bearing mice, respectively were confirmed with respect to the control group (20±0.13 days). The extract/fraction also decreased the body weight of the EAC tumor bearing mice. Hematological studies reveal that the heamoglobin (Hb) content was decreased in EAC treated mice whereas restoration to near normal levels was observed in extract treated animals. There was a significant (p<0.001) decrease in RBC count and increase in WBC counts in extract/fraction treated animals when compared to EAC treated animals. From the result it was showed that the extract has significant anticancer activity and that is comparable to that of Bleomycin. © 2011 Academic Journals Inc.
Aktar F.,University of Dhaka |
Kuddus M.R.,University of Dhaka |
Faruque S.O.,State University of Bangladesh |
Rumi F.,Manarat International University |
And 2 more authors.
Journal of Pharmacy and Nutrition Sciences | Year: 2011
The methanolic crude extract of the bark and seed of Entada phaseoloides (L.) Merr. and its different organic soluble partitionates were screened for antioxidant, cytotoxic, membrane stabilizing and antimicrobial activities. The crude extract and carbon tetrachloride and aqueous soluble fractions of both bark and seed showed higher level of total phenolic content (TPC, 245.59, 240.22, 240.03 & 117.0 mg of GAE/gm of dried extract). In the DPPH assay, the crude extract of bark and its chloroform and aqueous soluble fractions demonstrated strong antioxidant property with the IC50 of 3.24, 1.55 and 3.6 μg/ml, respectively whereas all the fractions of seed extract revealed mild antioxidant activity. The petroleum ether soluble fraction of both seed and bark exhibited significant cytotoxicity (LC50 = 1.54 & 5.4 μg/ml) which confers the presence of bioactive metabolites in this plant. On the other hand, the crude extract of seed and petroleum ether soluble fraction of bark inhibited the hemolysis of RBC of rat's blood by 78.89 and 57.43%, respectively as compared to 84.44% exerted by acetyl salicylic acid (0.10 mg/ml). In antimicrobial screening, the carbon tetrachloride soluble fraction of bark showed significant antimicrobial activity against Staphylococcus aureus (zone of inhibition = 17.0 mm) with MIC and MBC values of 7.81 μg/ml and 125 μg/ml, respectively. © 2011 Lifescience Global.
Shaheen S.M.,University of Rajshahi |
Shaheen S.M.,Bulgarian Academy of Science |
Jahan L.,University of Rajshahi |
Jahan L.,Manarat International University |
Ferdaus R.,University of Rajshahi
Pakistan Journal of Pharmaceutical Sciences | Year: 2015
In order to introduce an easily applicable, removable, painless and long-term drug delivery system for noninsulin dependent diabetes mellitus (NIDDM), hydroxyl propyl methyl cellulose with polyvinyl alcohol (HPMC-PVA) blend patches of metormin HCl were evaluated in vitro and in vivo. A suitable patch of metformin 800mg with HPMC-PVA blend were used, following a three cycle freeze-thaw technique. Drug release kinetic profiles were performed in both patch and swim bladder. Albino mice were artificially generated as NIDDM mice by alloxan insertion i.p and after then treated with the therapeutic patch. Blood glucose was estimated by commercially available glucose kit based on glucose oxidase method. Drug release parameters from the patch and swim bladder were typical non-Fickian diffusion and both have the same kinetic constant, revealing its possible diffusion through stratum corneum. Hypoglycemia was observed in treatment of normal mice with TDDS of metformin HCl within 4 hours i.e. 25±2.13mg/dl and within 16 hours in diabetic rats blood glucose level returned to normal level i.e.from 360±3.3mg/dl (NIDDM level) to 105±2.5 mg/dl (Normal level). The TDS-patch has got the same kinetic simulation with that of swim-bladder, which might be a prediction for in vivo application. Here metformin was delivered to diabetic mice and has got significant anti-diabetic effect can be considered as a kind of patch for NIDDM just like wearing and taking off a hand watch because hypoglycaemia can be removed by just taking off the patch.
Polyphenols content, cytotoxic, membrane stabilizing and thrombolytic activities of Sarcolobus globosus: A medicinal plant from Sundarban forest [Contenido de polifenoles, citotoxicidad, estabilizante de membranas y actividades tromboliticas de Sarcolobus globosus. Una planta medicinal de la Foresta Sundarban]
Kuddus M.R.,Manarat International University |
Aktar F.,University of Dhaka |
Miah M.K.,University of Dhaka |
Baki M.A.,University of Dhaka |
Rashid M.A.,University of Dhaka
Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas | Year: 2011
The crude methanolic extract of the bark of Sarcolobus globosus (Family-apocynaceae) and its different organic soluble Kupchan fractions were screened for total phenol content (TPC), cytotoxic, membrane stabilizing and thrombolytic activities. The polyphenol content was determined colorimetrically using Folin-Ciocalteu method and expressed in gallic acid equivalent. The chloroform soluble Kupchan fraction (CSF) exhibited higher level of Total Polyphenol Contents (TPC, 54.21 gm of GAE/100 gm of dried extract). In the brine shrimp lethality bioassay, the crude methanolic extract (MEBP) exhibited significant cytotoxicity. The membrane stabilizing activity was assessed by using erythrocyte in hypotonic solution and was compared with acetyl salicylic acid. The hexane soluble Kupchan fraction (HSF) produced 52.73 % inhibition of hemolysis of RBC as compared to 65.38 % revealed by acetyl salicylic acid (0.10 mg/mL). In thrombolytic study screening, the crude methanolic extract demonstrated significant thrombolytic activity in human blood specimen. © 2011 Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas.