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Guttikonda V.R.,Mamata Dental College and Hospital | Patil R.,Raja Rajeswari Dental College and Hospital | Kumar G.S.,K S R Institute of Dental science
Journal of Oral and Maxillofacial Pathology | Year: 2014

Aim : To Quantify the DNA single-stranded breaks in the peripheral blood leukocytes (PBLs) of tobacco-habituated individuals with clinically normal mucosa and patients with oral carcinoma.Materials and Methods : Patients who were attending the outpatient department were enrolled in this study. A control group of 30 healthy volunteers included in Group I were selected from various age groups who are not tobacco users in any form. Thirty patients with tobacco habituation but with clinically normal mucosa were included in Group II, while 30 tobacco-habituated patients with oral squamous carcinoma were included in Group III. A biopsy was taken from the representative area and confirmed histologically. Intravenous blood samples were collected from all the groups for evaluation of the extent of DNA damage using ethidium bromide-stained slides under fluorescent microscope. The DNA tail length was calculated by subtracting the diameter from the total length. Twenty-five randomly selected cells per slide were analyzed and mean calculated.Objectives: To evaluate DNA damage levels in PBLs of tobacco-habituated individuals with clinically normal mucosa and patients with oral carcinoma and compare with a control group of healthy volunteers. To evaluate the extent of DNA damage in PBLs using Single Cell Gel Electrophoresis (SCGE) in the above groups.Results : The mean DNA damage levels in patients with tobacco habits were compared with that of the control group and the results were found to be statistically significant. The mean DNA damage level in PBLs between tobacco-habituated patients with normal mucosa and oral cancer patients was found to be statistically significant. The DNA damage in cancer patients was compared with the control group and the results were found to be statistically significant.Conclusion: DNA damage evaluation in PBLs by SCGE technique is a sensitive and reliable indicator of tobacco insult.


Saraf K.,SMBT Dental College and Hospital | Shashikanth M.C.,JKK Nataraja Dental College and Hospital | Priya T.,Mamata Dental College | Sultana N.,MIDSR Dental College and Hospital | Chaitanya N.C.S.K.,Mamata Dental College and Hospital
Journal of Association of Physicians of India | Year: 2010

Probiotics are dietary supplements containing potentially beneficial bacteria or yeasts. They are administered in different quantities that allow for colon colonization. These products help in stimulating health promoting flora and also suppressing the pathologic colonization and disease spread. The use of probiotic plays an important aspect in dentistry too ever since the oral infections occupied the prime space among the other infections effecting the humans. This concept of microbial ecologic change both for medical and dental changes has accumulated a lot of evidence in recent times. But to date, no substantial literature and use has been postulated. There are claims that probiotics strengthen the immune system to combat allergies, stress, exposure to toxic substances and other diseases. There are reports of their beneficial use in HIV infection and cancers, mostly, the colo-rectal carcinomas. Their use in pre malignant and malignant oral disorders is yet to be probed. Strains of genera lactobacillus and bifidobacterium are the most widely used probiotic bacteria. This review is an attempt to discuss briefly the role of probiotics in medicine and dentistry. © JAPI.


Mohammed R.B.,Gitam Dental College and Hospital | Sanghvi P.,MGIMS | Perumalla K.K.,Mamata Dental College and Hospital | Srinivasaraju D.,Vishnu Dental College and Hospital | And 3 more authors.
Journal of Clinical and Diagnostic Research | Year: 2015

Introduction: For various forensic investigations of both living and dead individuals, the knowledge of the actual age or date of birth of the subject is of utmost importance. In recent years, age estimation has gained importance for a variety of reasons, including identifying criminal and legal responsibility, and for many other social events such as birth certificate, marriage, beginning a job, joining the army and retirement. Developing teeth are used to assess maturity and estimate age in number of disciplines; however the accuracy of different methods has not been assessed systematically. The aim of this study was to determine the accuracy of four dental age estimation methods.Materials and Methods: Digital Orthopantomographs (OPGS) of South Indian children between the ages of 6 and 16 y who visited the department of Department of Oral medicine and Radiology of GITAM Dental College, Visakhapatnam, Andhra Pradesh, India with similar ethnic origin were assessed. Dental age was calculated using Demirjian, Willems, Nolla, and adopted Haavikko methods and the difference between estimated dental age and chronological age were compared with paired t-test and Wilcoxon signed rank test.Results: An overestimation of the dental age was observed by using Demirjian and Nolla methods (0.1±1.63, 0.47±0.83 years in total sample respectively) and an underestimation of dental age was observed by using Willems and Haavikko methods (-0.4±1.53, -2.9±1.41 years respectively in total sample). Conclusion: Nolla’s method was more accurate in estimating dental age compared to other methods. Moreover, all the four methods were found to be reliable in estimating age of individuals of unknown chronological age in South Indian children. © 2015, Journal of Clinical and Diagnostic Research. All rights reserved.


Koora K.,Mamata Dental College and Hospital | Muthu M.S.,Meenakshi Ammal Dental College and Hospital | Rao C.,P.A. College | Sivakumar N.,Narayana Dental College and Hospital
Journal of Indian Society of Pedodontics and Preventive Dentistry | Year: 2010

Objective: The purpose of this study was to investigate the morphological characteristics of deciduous teeth of children in Chennai and Hyderabad, by establishing normal data on the mesiodistal and buccolingual crown dimensions in 3-5 year old children. Materials and Methods: Study casts of 200 children (100 boys and 100 girls) from two groups (Chennai and Hyderabad) were used in this study. Digital caliper was used to measure the mesiodistal and buccolingual dimensions. Results and Conclusion: Significant differences were found in the mean values of mesiodistal and buccolingual diameters of primary teeth, in which boys generally had larger crown diameters than girls.


Beeram R.K.,Mamata Dental College and Hospital | Seelam S.,Mamata Dental College and Hospital | Prasad L.K.,SIBAR Institution of Dental science
Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology | Year: 2016

Objective: Selection of the method of intubation during complex facial surgeries is a difficult task for the anesthetist because of airway is sharing area and oral or nasal intubation is not preferable, even though, tracheostomy is a worthy alternative; however, it has serious complications. Altemir FH introduced the 'submental technique' as a substitute for tracheostomy but it has demonstrated the complications of 'lingual nerve damage' and 'Wharton's duct injuries'. So, the Stoll's approach is the safest option in this scenario. The purpose of the present study was to evaluate 'Stoll's submandibular method' as a safest choice to Altemir's technique. Materials and method: The study was conducted prospectively in a sample of 42 patients for intra-operative airway management in complex facial surgeries during the period of six years from 2008 to 13 by considering the following objectives: duration of the procedure, and intra-operative and postoperative complications. Results: The average time taken for this procedure was 9 min. The complications encountered were superficial skin wound infection in a total of three patients (7%), partial extubation in one case (2%), cuff leakage in one case (2%) and dislodgement of the tube in one case (2%). Conclusion: The results of the present study have provided the evidence of Stoll's technique as a safest choice both for Altemir's and tracheostomy in justifiable circumstances because of simple, quick technique associated with negligible complications. So, anesthetist should consider this underutilized technique in complex facial surgeries and it is a valuable option in patients, who underwent tracheostomy in previous surgeries. © 2015 Asian AOMS, ASOMP, JSOP, JSOMS, JSOM, and JAMI.

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