Abbulu K.,Mallareddy institute of pharmaceutical science
Biomedicine and Preventive Nutrition | Year: 2013
The aim of the study was to develop self-microemulsifying drug delivery system (SMEDDS) of a poorly water soluble drug, pioglitazone. Approximately 40% of new drug candidates have poor water solubility and the oral delivery of such drugs is frequently associated with implications of low bioavailability, high intra- and intersubject variability and lack of dose proportionality. Hydrophobic drugs can often be dissolved in microemulsion allowing them to be encapsulated in the form of fine globules, so that drug remains undissolved in the gut avoiding the dissolution step, which frequently limit the rate of absorption of hydrophobic drugs. Phase solubility studies were conducted for the maximum solubility of pioglitazone. Highest was found in Tween 80 (surfactant) polyethylene glycol 400 (cosurfactant) and cottonseed oil. Ternary phase diagrams were constructed to evaluate microemulsion regions. FTIR analysis was done for investigating the drug-excipient interactions. The mean globule size of SMEDDS was observed to be below 200. nm for the optimized formulations and the zeta potential was negative. The dissolution of emulsion formulations was compared with commercial tablets; the results indicated that the rate of dissolution of developed formulations containing pioglitazone was 2 to 3 folds increased compared with that of commercial tablets. SEM studies were done for the shape and morphology of the globules. Thus, SMEDDS can be regarded as novel and commercially feasible alternative to the current pioglitazone formulations. © 2013 Elsevier Masson SAS.
Naidu L.V.,Mallareddy Institute of Pharmaceutical science
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2011
A transdermal drug delivery device, which may be of an active or a passive design, is a device which provides an alternative route for administering medication defined as self contained, discrete dosage forms which, when applied to the intact skin, deliver the drug, through the skin at controlled rate to the systemic circulation. These devices allow for pharmaceuticals to be delivered across the skin barrier. In theory transdermal patches work very simply. A drug is applied in a relatively high dosage to the inside of a patch, which is worn on the skin for an extended period of time. Through a diffusion process, the drug enters the bloodstream directly through the skin. Since there is high concentration on the patch and low concentration in the blood, the drug will keep diffusing into the blood for a long period of time, maintaining the constant concentration of drug in the blood flow.
Phanse M.A.,Jawaharlal Nehru Technological University |
Abbulu K.,Mallareddy Institute of Pharmaceutical science
Saudi Journal of Biological Sciences | Year: 2015
The present study is characterized toward thespesone isolation from . Thespesia populnea (Malvaceae). Subsequently it was modified and characterized to study its effect on diabetes related symptoms. The complex is administered to diabetes induced mice with the doses of 5, 10 and 20. mg/kg, p.o. and the effect of complex on the level of body weight, lipid profile and blood glucose was studied after 22. days. The results have indicated that diabetic mice show a significant (p . <. 0.01) decrease in the level of serum triglyceride, plasma glucose and increase in body weight. Hence the present investigation reveals that newly synthesized complex is useful in the management of Type-II diabetes mellitus because of its ability to reduce insulin resistance. © 2015.
Dasari R.,Mallareddy Institute of Pharmaceutical science
Journal of Chemical and Pharmaceutical Sciences | Year: 2016
Background: Dementia is a brain disorder characterized by decreased intellectual functioning interfering with the functions such as memory, language, perception, judgment and reasoning. Dementia is the main side effect in Alzheimer's disease patients mostly in older subjective. Objective: Objective: The following study was designed to assess the potential of Vanda spathulata as a nootropic agent and also to study its influence on brain cholinergic system of mice. Methods: The scopolamine and aluminum induced cognitive deficits models in mice were selected for the present study on elevated plus maze and Morris water maze test. A significant reversal effect was produced by Vanda spathulata extract measured the transfer latency on day 1 and day2 in scopolamine treated mice and it was also found that in Vanda spathulata extract on elevated plus maze decreased the cognitive deficits produced by sub-chronic administration of aluminum in mice. The animals showed a significant reduction in escape latency on days 2,3,4,5, 6, and 7 as compared with the control in Morris water maze. Results and Discussion: The activity of acetylcholinestarase enzyme was significantly increased in both scopolamine and aluminum induced mice. Whereas, decrease in the impairment of memory consolidation and also reduction in the activity of acetyl cholinesterase was observed upon the administration flowers of Vanda spathulata methanolic extract simultaneously with aluminum and scopolamine. Vanda spathulata produced the levels of AChE were decreased significantly in the whole brain homogenate. Hence the flowers of Vanda spathulata methanolic extract could be useful in various cognitive disorders like dementia and Alzheimer's disease.
Kumar R.,Mallareddy Institute of Pharmaceutical science
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2010
Attempts were made to prepare fast dissolving tablets of Chlorthalidone by using superdisintegrant, co-grinding with carriers (i.e. Mannitol, PVP), and solid dispersions with the same and by sublimation method. Formulations were evaluated for precompressional parameters such as angle of repose, % compressibility and Hausner's ratio. Tablets were subjected to postcompressional analysis for the parameters such as hardness, friability, in vitro disintegration time, wetting time and dissolution test. Drug compatibility with excipients was checked by FTIR and DSC studies. Stability studies were carried out as per ICH guidelines for three months. The results revealed that tablets prepared by the sublimation method using 40% camphor (F12) significantly enhanced the dissolution rate of drug. Tablets prepared by co-grinding with PVP without Crosspovidone gave inferior dissolution rates. Tablets containing solid dispersions with crospovidone yielded good results in terms of dissolution rate. Mannitol can successfully be used as carrier for both methods. Stability studies revealed that upon storage, disintegration time of tablets prepared with Mannitol increased significantly (p<0.05) and that those of prepared with PVP decreased significantly (p<0.05).
Sujatha S.,Mallareddy Institute of Pharmaceutical science
European Journal of Drug Metabolism and Pharmacokinetics | Year: 2015
The aim of present study was to investigate the effects of apigenin and rutin on the pharmacokinetics of paclitaxel after oral administration of paclitaxel with apigenin and rutin to rats. Paclitaxel (40 mg/kg) was administered orally alone and in combination with apigenin and rutin (10, 20, and 40 mg/kg) for 15 consecutive days. In the single-dose pharmacokinetic study (SDS), blood samples were collected on 1st day whereas on 15th day in the multiple-dose pharmacokinetic study (MDS). The plasma concentrations of paclitaxel were increased dose-dependently in the combination of apigenin and rutin compared to that of paclitaxel control in SDS and MDS (p < 0.01). The areas under the plasma concentration-time curve (AUC) and the plasma peak concentrations (C max) of paclitaxel with apigenin and rutin were significantly higher (p < 0.01) than that of the control. The AUCs and C max of paclitaxel were increased with apigenin and rutin in the dose-dependent manner. The half-life (t 1/2) was significantly longer than that of the control. Non-everted sacs were filled with paclitaxel 100 μM in the presence and absence of verapamil (50 μM), apigenin, and rutin (50, 100 μM) and incubated at 37 C for 60 min. The absorption of paclitaxel was increased in the presence of apigenin, rutin, and verapamil, a typical P-glycoprotein and Cyp3A4 inhibitor. If these results are confirmed in humans in a clinical setting, the paclitaxel dose should be adjusted when it is given concomitantly with apigenin and rutin. © 2014 Springer International Publishing Switzerland.
PubMed | Jawaharlal Nehru Technological University and Mallareddy Institute of Pharmaceutical science
Type: Journal Article | Journal: Saudi journal of biological sciences | Year: 2016
The present study is characterized toward thespesone isolation from Thespesia populnea (Malvaceae). Subsequently it was modified and characterized to study its effect on diabetes related symptoms. The complex is administered to diabetes induced mice with the doses of 5, 10 and 20mg/kg, p.o. and the effect of complex on the level of body weight, lipid profile and blood glucose was studied after 22days. The results have indicated that diabetic mice show a significant (p<0.01) decrease in the level of serum triglyceride, plasma glucose and increase in body weight. Hence the present investigation reveals that newly synthesized complex is useful in the management of Type-II diabetes mellitus because of its ability to reduce insulin resistance.
Sruthi T.,P.A. College |
Satyavati D.,Sree Dattha Institute of Pharmacy |
Dasari R.,Mallareddy Institute of Pharmaceutical science |
Jyothi V.,P.A. College |
Roshan Ali P.,P.A. College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2014
Objective: The present study was focused to evaluate the hypoglycemic activity of NIDDWIN, a polyherbal formulation in normal rats. Methods: Male Albino Wistar Rats (180-200gms) were divided into four groups of five animals each. Group-I was given aqueous suspension of 2% gum acacia, Group-II was given aqueous suspension of NIDDWIN 50mg/kg, Group-III was given aqueous suspension of NIDDWIN 100mg/kg, Group-IV was given aqueous suspension of Glibenclamide 10mg/kg were given orally for 10days. The blood samples were collected before and after administration drugs at 0hrs, 2hrs, 4hrs, 6hrs, and 8hrs on 1st, 5th, and 10th days from retro-orbital sinus and serum was separated and estimated for glucose, cholesterol and triglycerides by using analytical method[1,2]. Results: NIDDWIN showed significant hypoglycemic activity at 4hrs on 1st, 5th, and 10th days was found to be effect in comparable with standard Glibenclamide 10mg/kg. Conclusion: NIDDWIN a polyherbal formulation concluded that it possesses hypoglycemic activity in normal rats and should be evaluated for its antidiabetic activity.