Malankara Catholic College

Tamil Nadu, India

Malankara Catholic College

Tamil Nadu, India
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Joseph B.,Interhsciplinary Research Center | Nair V.M.,Malankara Catholic College
American Journal of Drug Discovery and Development | Year: 2012

In developing countries, typhoid fever has a substantial negative socioeconomic impact. Highly invasive organism, Salmonella typhi is the causative agent for this enteric fever. The virulence of Salmonella is associated with the presence of a capsular polysaccharide, Vi antigen. The Vi polysaccharide biosynthesis protein, tviC, of Salmonella typhi whose three dimensional structure was not elucidated till date and its sequence was retrieved from KEGG database. Homology modeling was performed using Swiss model and the resultant structure was verified using WHATCHECK tool. Docking studies were done on the modeled structure with plant derived inhibitors such as allicin, apigenin, caffeic acid, curcumin, eugenol, piperin and luteolin. The hydrogen bond interactions between the protein and ligand were visualized by PYMOL software. Docking studies revealed the e-value for eugenol (-329) is better than the other selected ligands. © 2012 Academic Journals Inc.

Borgio J.F.,St. Joseph's College | Bency B.J.,Malankara Catholic College | Thorat P.K.,Hardikar Hospital Campus | Lonkar A.D.,Hardikar Hospital Campus
American Journal of Drug Discovery and Development | Year: 2011

The present study deals with the effect of Gynandropsis pentaphylla (leaves and seeds) extracts (acetone, benzene, methanol, ether and water) on the production of extra and intracellular protein in 6 bacteria and 4 fungi. Effects of different type of extracts were done through the estimation of intra and extra cellular proteins content. Acetone extract of seeds inhibited the extra cellular protein synthesis at maximum level (0.072±0.02 mg L-1) in Bacillus subtilis NCIM 2010, followed by benzene extract of seeds which inhibited the synthesis of extra cellular protein (0.083±0.03 mg mL-1) in Escherichia coli NCIM 2064. Water extract of seeds suppressed the extra cellular protein synthesis at maximum level (0.296±0.08 mg mL-1) in Aspergillus niger NCIM 501. Intracellular protein synthesis was highly (0.285±0.12 mg mL-1) affected after the addition of methanol extracts of leaves in Staphylococcus aureus NCIM 2120. Intracellular protein synthesis was highly (0.169±0.09 mg mL-1) affected after the addition of benzene extracts of seed in Penicillium notatum NCIM 747. Sucrose, D-mannose and L-rhamnose were observed in the water extracts of leaves and seeds, when screened using TLC. Water extracts of leaves showed arginine, proline and methionine, while seeds showed proline and tyrosine. © 2011 Academic Journals Inc.

Shafi Thompson T.,Mar Ivanios College | Ashok A.,Malankara Catholic College | Sukesh K.,Malankara Catholic College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

This study focused on checking antibacterial effect of Psidium gaujava against dental caries forming oral flora. The antimicrobial activity of Psidium guajava was checked with ethanol, acetone, chloroform, methanol and water extracts against selected bacterial isolates. The acetone and methanol extract showed maximum inhibitory activity but ethanol extract inhibit only the growth of Pseudomonas aeruginosa and the water extract revealed high activity against both Streptococcus viridans and Bacillus megaterium. In phytochemical screening, the acetone and ethanol extracts gave positive results for steroids, terpenoids and flavonoids. Phenolic compounds were there only in acetone extract. Saponins were absent in ethanol extract and tannins simply present in acetone and methanol extract. Phytochemical analysis was done by Thin Layer Chromatography (TLC). Acetone solvent system produced three spots with highest Rf value 0.722. The chloroform solvent system produced three spots with highest Rf value 0.780. But the water, ethanol and methanol solvent system developed only two spots.

Joseph B.,The Interdisciplinary Center | Nair V.M.,Malankara Catholic College
International Journal of Pharma and Bio Sciences | Year: 2013

Presently, researchers have been given remarkable attention to complementary and alternative medicine to deal with cancer treatment from medicinal plants mainly due to its fewer side effects and ease availability. Several scientific studies deep- rooted the anti- cancerous property of Ocimum sanctum Linn., a traditional medicinal plant commonly known as Tulsi. The chemopreventive and radiopreventive property of O. sanctum along with its anti- oxidant, anti- inflammatory and anti- stress property reside as a backbone for it anti- cancerous effect. It is demonstrated that the cornerstone behind this effect is the various phytochemical constituents such as eugenol, orientin, vicenin- 2, linolenic acid and ursolic acid. The present review is an effort to amalgamate the various scientific studies underlying the anti-cancerous effect of O. sanctum under one roof. In this review, the anti-cancerous activity of O. sanctum in numerous cancers such as lung, skin, oral, cervical, gastric, breast and prostate were comprehensively represented.

Joseph B.,Malankara Catholic College | Raj S.J.,Malankara Catholic College
International Journal of Biological Chemistry | Year: 2011

The present investigation was carried out to understand the impact of curacron on Serum aminotransferases activity (ALT and AST) of fresh water fish Cyprinus carpio. Curacron is an organophosphate pesticide and is widely used by the farmers to protect their crops. So every year huge volume of Curacron enters to aquatic ecosystems. The objective of this study was to determine the effect of curcuron pesticides and its influence of serum biomarker enzyme (ALT and AST). LC 50of Curacron for Cyprinus carpio has been calculated by the log-dose/probit regression line method and recorded as 0.38 ppm at 96 h. Three sub-lethal concentrations (0.1, 0.01 mL and 0.001 ml L -1) were selected to expose the fish for 1,7, 14 and 21 days. Changes in enzyme activity were observed with all concentrations and exposure period. ALT and AST concentrations were significantly higher in the experimental group, compared with the control group. The above results of blood plasma profile indicate a marked cytotoxic and hepatotoxic effect of curacron in Cyprinus carpio. © 2011 Academic Journals Inc.

Prakash Williams G.,Malankara Catholic College | Gnanadesigan M.,Alagappa University | Ravikumar S.,Alagappa University
Bioresource Technology | Year: 2012

The present study is an attempt to find out the promising metal (Ni 2+, Al 3+, and Hg 2+) biosorbant halobacterial strains from saltern pan and to study their biokinetic parameters for metal recovery. Of the selected 65 isolates, Natronobacterium magadii, Natronococcus occultus and Halobacterium sodomense shows the maximum metal resistance activity against Ni 2+ (250ppm), Al 3+ (600ppm) and Hg 2+ (25ppm) metals. Biokinetic parameters such as maximum specific growth rate (μ max), substrate removable [S] rate, half velocity constant (K s), endogenous or decay co-efficient (k d), yield co-efficient rate (Yd -1) are also analyzed for the selected metals. Further the results of the SEM-EDS analysis confirms the metals uptake of Ni 2+ and Al 3+ in higher concentration and Hg 2+ in lower concentration. It is concluded from the present study that, the biosorption of heavy metals by solar saltern halobacteria can be used as a potential source for the bioremediation of metals. © 2011 Elsevier Ltd.

Bright K.C.,Malankara Catholic College | Freeda T.H.,Physics Research Center
Physica B: Condensed Matter | Year: 2010

Single crystals of l-alanine cadmium chloride (LACC), an organometallic nonlinear optical material, have been grown by the slow evaporation technique. The grown crystals were subjected to various characterization techniques, such as single crystal and powder XRD, FTIR, UVvis and TGA-DTA. The mechanical properties of the crystals show that this material belongs to the category of hard materials. Second harmonic generation was confirmed by the Kurtz and Perry powder technique. Electrical parameters, such as dielectric constant, dielectric loss, ac and dc conductivity and their corresponding activation energies have been studied. The low dielectric constant and dielectric loss suggest that this material is a good candidate for micro-electronic applications. © 2010 Elsevier B.V. All rights reserved.

Hema T.A.,Malankara Catholic College | Helen Pappa T.,Malankara Catholic College
International Journal of Pharma and Bio Sciences | Year: 2012

Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. Pseudomonas sp. MCCMB3 an alpha galactosidase producer was isolated from the rock soil of Kulasekaram, Kanyakumari district, South India. Totally 10 strains were isolated, when compared with other strains, predominant enzyme activity has been observed in the strain of Pseudomonas sp. MCCMB3. The maximum amount of enzyme was produced using nutrient broth during optimization. Environmental and nutritional parameters were optimized for the enhanced enzyme production. The enzyme production was maximum at pH 8.0 and temperature 50°C. Glucose and ammonium carbonate induces enzyme production as carbon and nitrogen sources respectively. Based on the zymographic analysis, the molecular weight of the purified enzyme was about 80 KDa. The alpha galactosidase enzyme produced by Pseudomonas sp MCCMB3 degrades raffinose and stachyose present in soymilk which is used in other industrial applications.

Hema T.A.,Malankara Catholic College | Arya A.S.,Malankara Catholic College | Suseelan S.,Malankara Catholic College | John Celestinal R.K.,Malankara Catholic College | Divya P.V.,Malankara Catholic College
International Journal of Pharma and Bio Sciences | Year: 2013

The antimicrobial activity of leaves of five South Indian medicinal plants Adhatoda vasika, Bacopa monnieri, Carica papaya, Cissampelos pareira and Cynodon dactylon, collected from regions of Kulathoor and Malayadi were investigated against ten clinical pathogens (Bacillus subtilis, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella typhi, Shigella sp., Staphylococcus aureus and Streptococcus sp.) using agar well diffusion method and broth dilution method. The plant extracts were prepared using the solvents - acetone, ethanol and propanol. It is clear from the results that, the extract of five plants used in this study acts as a good source of antibiotics against various bacterial pathogens tested and exhibited a broad spectrum of antimicrobial activity. The phytochemical analysis revealed the presence of alkaloids in all plants selected and other secondary metabolites like tannins, glycosides and saponins were also observed in all the extracts. The MIC values ranges between 3.175(g/ml and 12.5(g/ml, whereas MBC values ranges between 6.25(g/ml and 12.5(g/ml. The results of this study support the use of all the selected five medicinal plants to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals that address unmet therapeutic needs.

Sukanya M.K.,Malankara Catholic college | Suku S.,Malankara Catholic college | Aruna S.R.,Malankara Catholic college
International Journal of Pharma and Bio Sciences | Year: 2013

In our study, preliminary phytochemical analysis, antimicrobial and antihelminthic investigation on Phyllanthus emblica was done. P. emblica is a well known and an important medicinally valued plant. Although the efficacy of P. emblica fruit is widely proved, use of leaf and bark is less investigated. Present aim of our study is to find the biologically active compound present in this particular plant, check its antimicrobial and antihelminthic property. Different solvent extract of the leaf and bark were used to identify the bioactive compounds present and its antimicrobial activity was checked against different human pathogens(MTCC).Antihelminthic activty was checked against Phertima posthuma. The study shown it has promising antimicrobial and antihelminthic property. Methanol of the leaf sample showed highest zone of inhibition against Enterobacter aerogens and Enterobacter feacalis. Ethyl acetate of leaf and bark sample showed antifungal activity against Rhizomucor species. The pharmacological property of this medicinally important plant has to be further investigated.

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