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New Delhi, India

Kumar R.,Maize Research
Journal of Environmental Biology | Year: 2015

Genetic diversity of 49 maize inbreds was assessed using twelve ISSR primers. A wide variation in PCR products was revealed in terms of size (280 to 3000bp), extent of polymorphism (94.87%) and number of bands (4-9). As a whole, 78 ISSR bands were produced (including four monomorphic bands) with an average of 6.5 bands per primer and the maximum number of bands (9) being produced by primer OUAT-8. Five ISSR primers (OUAT-8, OUAT-9, OUAT-15, OUAT 17 and OUAT-18) revealed higher PIC value (around 0.70) along with 100% polymorphism indicating better allelic diversity. While, ISSR primer OUAT- 15 revealed higher number of polymorphic bands (8) with 100% polymorphism as well as considerably high PIC and Rp values. Thus, such an informative and discriminative primer is of immense value for the study of genetic diversity in a set of maize genotypes. The similarity index values ranged from 0.3 to 0.9 with an average of 0.522 and BQPM-1-14 maintained the highest genetic distance as revealed from its lowest average similarity coefficient value (0.393) with rest of the genotypes. BQPM 1-14, BQPM 1-8, BQPM 3- 10, BQPM 6-8 and B 1110-7-2 were identified to be highly divergent among the test inbreds which could be sorted out as valuable materials for heterosis breeding for production of single cross hybrids. © Triveni Enterprises, Lucknow (India).

Parihar A.K.,Indian Institute of Pulses Research | Parihar C.M.,Maize Research
Indian Journal of Genetics and Plant Breeding | Year: 2012

The effects of different moisture stress conditions on performance of 85 quality protein maize (QPM) genotypes were measured. Significant variations among the genotypes were observed for grain yield/plant and quality traits (protein, lysine. tryptophan).The study showed that hybrids NP-06-07R-74-10-1-1 × HO6R-6136-64-1-4-1 and NP-06-07R -73-6-1#1 × HO6R-6136-64-1-4-1 gave superior performance in all the environments with respect to yield, economic heterosis and percentage loss in stress. The inbreds viz., (CML 161 × CLQ-6203) B-4-1-1-B-2-B-1 and HO6R-6136-64-1-4-1 were best for grain yield, while, the inbreds NP-06-07R -76-11-2-1, NP-06-07R -73-6-1#1 and (CML 161 × CLQ-6203)B-4-1-1-B-2-B-1 were best for both lysine and tryptophan. The correlation between different moisture stress conditions and quality traits was positive. A single cross hybrid NP-06-07R -73-6-1#1 × HO6R-6136- 64-1-4-1 was identified as superior for grain yield/plant, lysine and tryptophan contents in all the environments as compared to best check HQPM-5.

Chandra A.,Maize Research | Singh S.B.,Indian Agricultural Research Institute | Kumar P.,Maize Research
Environmental Monitoring and Assessment | Year: 2013

2,4-Dihydroxy-7-methoxy-1,4-benzoxazine-3-one (DIMBOA) known for its antibiotic properties against many pests and diseases in maize plant was extracted from different varieties. In one of the extraction methods of DIMBOA from maize leaves, Amberlite XAD7, an adsorbent, was used for extracting this and it gave a pure fraction containing DIMBOA. The adsorbent Amberlite XAD, being an expensive material, was uneconomical for processing a large number of samples. Therefore, one of the reported solvent extraction method was modified to make the extraction procedure cost effective without losing the target compounds. Using the modified method, DIMBOA was extracted for different varieties under dark and light condition. Results showed that high concentrations of DIMBOA were extracted from plants grown in dark condition as compared to plants grown in light indicating that light played significant role in inhibition of DIMBOA. © 2013 Springer Science+Business Media Dordrecht.

Kumar L.,Research Center for Makhana | Choudhary A.K.,Research Center for Makhana | Bhatt B.P.,Research Center for Makhana | Bhatt B.P.,Maize Research | Singh K.P.,Research Center for Makhana
Indian Journal of Horticulture | Year: 2015

Makhana (Euryale ferox Salisb.) is an important aquatic cash crop of eastern India. During 2009-10 and 2010-11, an attempt was made to study genetic divergence among 36 makhana genotypes using Mahalanobis D2-statistic at ICAR-RCER, Research Centre for Makhana, Darbhanga (Bihar). Experiments were conducted in field condition in Randomized Block Design with three replications. Data were recorded on 14 morpho-physiological traits, viz., days to germination, days to initiation of flowering, days to 50% flowering, days to 100% flowering, days to initiation of fruit bursting, leaf diameter (cm), fruit diameter (cm), fruits per plant, seeds per fruit, seeds per plant, seed yield per fruit (g), seed yield per plant (g), 100-seed weight (g) and seed diameter (cm). On the basis of D2 values, these genotypes were grouped into seven clusters. The clustering pattern indicated that cluster I and cluster II were the largest ones accommodating ten genotypes each. While clusters IV and VI were observed the smallest one with single genotype. The highest inter-cluster D2 values was observed between cluster III and VII (423.20) followed by cluster III and IV (379.69), indicating wider genetic diversity among the groups. The highest intra-cluster D2 values were observed for the cluster VII (75.90) and the lowest in cluster IV and VII (0.00). Among 14 traits studied, 100-seed weight was observed to have highest contribution (57.30%) towards genetic divergence followed by seeds/ fruit (18.25%) and fruit diameter (17.46%). The results suggested that genotypes BR-5 of cluster III and BR-1 of cluster VII could be the most suitable parents for development of high yielding varieties of makhana through hybridization. © 2015, Horticulture Society of India. All rights reserved.

Khokhar M.K.,Maize Research | Hooda K.S.,Maize Research | Sharma S.S.,Maharana Pratap University of Agriculture and Technology | Singh V.,Maize Research
Maydica | Year: 2014

Post flowering stalk rot complex is one of the most serious, destructive and widespread group of diseases in maize and yield losses range from 10 to 42% and can be as high as 100% in some areas. PFSR nature is often complex as a number of fungi (like Fusarium verticillioides cause Fusarium stalk rot, Macrophomina phaseolina cause charcoal rot, Harpophora maydis cause late wilt) are involved in causation of the diseases. To combat this problem, identification of quantitative trait loci for resistance to PFSR would facilitate the development of disease resistant maize hybrids. Moreover, various chemical and biological control methods have been developed but major emphasis is on development of maize cultivars with genetic resistance to for environment friendly control of the Post flowering stalk rot complex. The current paper reviews the information on distribution, impact of the disease, symptoms, epidemiology, disease cycle; genetics of resistance and integrated disease management approaches has been enumerated to understand the present status of knowledge about PFSR complex and will try to focus on the future perspectives available to improve PFSR management. © 2014 Consiglio per la Ricercame la sperimentazione in Agrcoltura. All rights reserved.

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