Mahendra Arts and Science College Autonomous

Nāmakkal, India

Mahendra Arts and Science College Autonomous

Nāmakkal, India
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Govarthanan M.,Muroran Institute of Technology | Mythili R.,Mahendra Arts and Science College Autonomous | Selvankumar T.,Mahendra Arts and Science College Autonomous | Kamala-Kannan S.,Chonbuk National University | And 2 more authors.
Biotechnology and Bioprocess Engineering | Year: 2017

Objective of the study was to isolate heavy metal resistant bacteria from chromium-contaminated subsurface soil and investigate biosurfactant production and heavy metal bioremediation. Based on 16S rRNA gene sequence and phylogenetic analysis, the isolate was identified as Rahnella sp. RM. The biosurfactant production by heavy metal resistant Rahnella sp. RM was optimized using Box- Behnken design (BBD). The maximum emulsification activity was obtained 66% at 6% soybean meal in pH 7.0 and 33.5°C. The biosurfactant was characterized using Field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FT-IR) and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF). The highest metal removal rates using the biosurfactant were found 74.3, 72.5, and 70.1%, respectively, at the 100 mg/L amended flasks at 48 h. This study indicated the biosurfactant from heavy metal resistant Rahnella sp. RM could be used as a potential tool to remediate the metals in contaminated environments. © 2017, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.


Prabakaran K.,Mahendra Arts and Science College Autonomous | Rajesh K.B.,Government of Tamilnadu | Chandrasekaran R.,Government of Tamilnadu | Sarasvathi R.C.,Government of Tamilnadu
Journal of Optics (India) | Year: 2015

We propose a new approach for generating multiple focal spots segment of sub wavelength size, by tight focusing of phase modulated double ring shaped radially polarized beam. The focusing properties are investigated theoretically by vector diffraction theory. We proposed to design of binary phase plate (BPP) which can tune the phase of the incident RTEM11 * mode radially polarized Laguerre –Gaussian beams (R-TEM11*) beam in such way to generate a focal segment with four and three numbers of focal spots repectively. We also observed that by properly tuning the pupil to beam ratio(β) of the incident R-TEM11* beam, the generated four or three focal spot segment can be tuned to a focal segment of two focal spot. © 2015, The Optical Society of India.


Sri Ramkumar S.R.,Bharathiar University | Sivakumar N.,Madurai Kamaraj University | Selvakumar G.,Alagappa University | Selvankumar T.,Mahendra Arts and Science College Autonomous | And 3 more authors.
RSC Advances | Year: 2017

Nanoparticle biosynthesis using the extract of medicinal plants in a non-hazardous mode has gained wide attention for various applications in nanomedicine. This study aimed to synthesize silver nanoparticles (AgNPs) using the aqueous extract of the Garcinia imberti and to evaluate their antibacterial and cytotoxic activities. Structural characterization of synthesized AgNPs was carried out using UV-Vis spectrophotometry, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). SEM showed the formation of particles with average sizes of 25 to 40 nm. Energy dispersive spectroscopy (EDS) was used to analyze the AgNPs within the energy range of 3-3.1 keV to detect the presence of silver. Also, the presence of the face centered solid cubic crystal structure of metallic silver was confirmed with X-ray diffraction (XRD). The functional groups of the biomolecules present in the aqueous extract of G. imberti and their interaction with AgNPs were identified through FT-IR analysis. The biosynthesized AgNPs exhibited antibacterial activity against root canal isolates of Staphylococcus sciuri S5, Enterococcus faecalis S9 and Enterococcus faecium S11. The green synthesized AgNPs showed no significant effects on HepG2 cell lines at 75 μg mL-1. Thus, the biosynthesized AgNPs are found to have potential for nano-based drug delivery in future root canal treatment applications. © 2017 The Royal Society of Chemistry.


Muthusamy G.,Mahendra Arts and Science College Autonomous | Muthusamy G.,University of Seoul | Thangasamy S.,Mahendra Arts and Science College Autonomous | Raja M.,Mahendra Arts and Science College Autonomous | And 2 more authors.
Environmental Science and Pollution Research | Year: 2017

The present work focuses on a low-cost, simple, and green synthesis of silver nanoparticles (AgNPs) by mixing AgNO3 solution with the extract of Spirulina platensis (SP) without any chemical reducing and/or capping agents. The green synthesis of AgNPs was confirmed by the color change from colorless to yellowish brown. The biosynthesis of AgNPs was further confirmed by UV-visible spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), biological transmission electron microscopy (Bio-TEM), and energy dispersive X-ray analysis (EDX). The UV-vis spectroscopy results showed the surface plasmon resonance (SPR) of AgNPs around 450 nm. Bio-TEM analysis revealed that the Ag nanoparticles were well dispersed with average range of 5–50 nm. XRD results indicated that the green synthetic process produced face-centered cubic structure of AgNPs. FT-IR spectroscopy analysis showed that the bioactive molecules from the SP extract believed to be the responsible for the reduction of Ag ions. Furthermore, the synthesized AgNPs were evaluated against pathogens such as Staphylococcus sp. and Klebsiella sp. The AgNPs (1–4 mM) extensively reduced the growth rate of the pathogens. © 2017 Springer-Verlag GmbH Germany


Selvankumar T.,Mahendra Arts and Science College Autonomous | Radhika R.,Mahendra Arts and Science College Autonomous | Mythili R.,Mahendra Arts and Science College Autonomous | Arunprakash S.,Arignar Anna Government Arts College | And 4 more authors.
3 Biotech | Year: 2017

The aim of the present study was to assess the arsenic (As) transformation potential of endophytic bacteria isolated from roots of Pteris vittata plant. The endophytic bacterium was tested for minimal inhibitory concentration (MIC) against As. The endophytic strain RPT exhibited the highest resistance to As(V) (400 mg/l). Phylogenetic analysis of the 16S rRNA sequence suggested that strain RPT was a member of genus Citrobacter. The As transformation assay revealed As(III) oxidation and As(V) reduction potential of Citrobacter sp. RPT. The As resistance mechanism was further confirmed by amplification of arsC and aoxB genes. The growth kinetics of strain RPT was altered slightly in the presence of different concentration (100–400 mg/l) of As stress. Temperature and pH influenced the As removal rate. The maximum As removal was observed at pH 7.0 (74%) and 37 °C (70.9%). The results suggest that strain RPT can survive under the As stress and has been identified as a potential candidate for application in bioremediation of As in contaminated environments. © 2017, Springer-Verlag GmbH Germany.


Sundaram C.M.,Periyar University | Prabakaran K.,Mahendra Arts and Science College Autonomous | Anbarasan P.M.,Periyar University | Rajesh K.B.,Government of Tamilnadu | Musthafa A.M.,Royal University
Chinese Physics Letters | Year: 2016

The intensity distribution in the focal region of a high-NA lens for the incident azimuthally polarized multi Gaussian beam transmitted through a multi belt spiral phase hologram is studied on the basis of the vector diffraction theory. Here we report a new method used to generate a needle of transversely polarized light beam with sub diffraction beam size of 0.366λ that propagates without divergence over a long distance of about 22λ in free space. We also expect that such a light needle of transversely polarized beam may find its applications in utilizing optical materials or instruments responsive to the transversal field only.


Chandrasekaran R.,Government of Tamilnadu | Prabakaran K.,Mahendra Arts and Science College Autonomous | Rajesh K.B.,Government of Tamilnadu | Mohamed Musthafa A.,Royal University | Ravi V.,Government of Tamilnadu
Optik | Year: 2016

Focusing properties of cylindrically polarized annular multi gaussian beam with high numerical aperture lens system is investigated theoretically by vector diffraction theory. Results show that intensity distribution in focal region can be tailored considerably by appropriately adjusting the polarization angle. Peak-centered, donut and flattop focal shapes with extended focal depth, potentially useful in optical tweezers, material processing and laser printing can be obtained using this technique. © 2016 Elsevier GmbH. All rights reserved.


Chandrasekaran R.,Government of Tamilnadu | Prabakaran K.,Mahendra Arts and Science College Autonomous | Rajesh K.B.,Government of Tamilnadu
Optical and Quantum Electronics | Year: 2016

We investigated the focusing properties of a cylindrically polarized annular multi Gaussian beam tightly focused with a high NA lens and a complex phase filter. It is observed that a properly designed complex phase filter can generate multiple focal spot and focal hole segments along optical axis its useful for the manipulation of multiple optical traps of low and high refractive index particles. © 2015, The Author(s).


Praburaman L.,Chonbuk National University | Park J.-H.,Chonbuk National University | Govarthanan M.,Chonbuk National University | Selvankumar T.,Mahendra Arts and Science College Autonomous | And 5 more authors.
Chemosphere | Year: 2015

The study was aimed to evaluate the potential of organic formulation, panchakavya, for enhancing the biological leaching of Pb and Cu in contaminated mine soil. Response surface methodology based Box-Behnken design was used to optimize the variables such as incubation time, panchakavya concentration, and agitation rate. The maximum bioleaching (Pb=64% and Cu=49%) was observed after 54h of incubation with 10mL panchakavya at 120rpm. Statistics-based contour and three-dimensional plots were generated to understand the relationship between Pb and Cu bioleaching and variables. High-performance liquid chromatography analysis showed the presence of lactic (25.88mgg-1), citric (0.14mgg-1), succinic (0.14mgg-1), malic (0.66mgg-1), and acetic (0.44mgg-1) acids in panchakavya, which may have a vital role in the removal of metals from the contaminated soil. Soil fraction studies indicate a significant increase of Pb (45%) in the exchangeable fraction of panchakavya-treated soil. XRD studies confirmed the role of panchakavya induced calcite and other minerals in the precipitation of metal ions. A significant increase in the enzyme activities of phosphatase, dehydrogenase, urease, amylase, invertase, and cellulase were observed in the panchakavya-treated soil. © 2015 Elsevier Ltd.


PubMed | Mine Reclamation Corporation, Chonbuk National University and Mahendra Arts and Science College Autonomous
Type: | Journal: Chemosphere | Year: 2015

The study was aimed to evaluate the potential of organic formulation, panchakavya, for enhancing the biological leaching of Pb and Cu in contaminated mine soil. Response surface methodology based Box-Behnken design was used to optimize the variables such as incubation time, panchakavya concentration, and agitation rate. The maximum bioleaching (Pb=64% and Cu=49%) was observed after 54 h of incubation with 10 mL panchakavya at 120 rpm. Statistics-based contour and three-dimensional plots were generated to understand the relationship between Pb and Cu bioleaching and variables. High-performance liquid chromatography analysis showed the presence of lactic (25.88 mg g(-1)), citric (0.14 mg g(-1)), succinic (0.14 mg g(-1)), malic (0.66 mg g(-1)), and acetic (0.44 mg g(-1)) acids in panchakavya, which may have a vital role in the removal of metals from the contaminated soil. Soil fraction studies indicate a significant increase of Pb (45%) in the exchangeable fraction of panchakavya-treated soil. XRD studies confirmed the role of panchakavya induced calcite and other minerals in the precipitation of metal ions. A significant increase in the enzyme activities of phosphatase, dehydrogenase, urease, amylase, invertase, and cellulase were observed in the panchakavya-treated soil.

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