Mahatma Jyoti Rao Phoole University

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Jaipur, India
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Mishra R.,Mahatma Jyoti Rao Phoole University | Jain S.,Sagar Institute of Research and Technology Pharmacy
Pharmacologyonline | Year: 2013

Twenty thiazolyl chalcones were tested for their ability to inhibit the growth of Plasmodium facliparum in vitro by using the candle jar method for antimalarial assay. The percent inhibiton of parasitemia was calculated from blood smears stained with geimsa stain. The best compounds had chloro or methoxy group in the para position of the phenyl ring B of the molecule.


Mallik S.,Mahatma Jyoti Rao Phoole University | Kshirsagar M.D.,P.A. College | Saini V.,MM University
Der Pharmacia Lettre | Year: 2011

Stability of Pharmaceutical formulations are oftenly challenged by compatibility between drugs and excipients. The objective of the present study is to identify compatibility between drug:drug and drugs:excipient. Curcumin and Tretinoin were selected as model drug where Ethyl cellulose (EC) and Poly vinyl alcohol (PVA) as excipients. The study was done by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction study (XRD) and Thin layer chromatography (TLC). The spectrophotometric graphs revealed that there was no significant changes in position of functional groups of Curcumin, Tretinoin, EC and PVA (O-H, C=O, C-H str.) in pure drugs and excipients with respect to their physical mixtures. X-ray diffraction study reflects that the characteristic peaks of curcumin appeared at a diffraction angle of 2□ at 7.950, 8.800, 12.280, 17.290 showing that Curcumin and Tretinoin was present as a crystalline form as well. The Rf values of the physical mixtures obtained from TLC study on the 0th and 30th day were approximately similar to the Rf values of the pure Curcumin and Tretinoin. The above spectrophotometric data and retention factor clearly implies that the synthetic-herbal drug combinations with pharmaceutical excipients are compatible with each other and can be introduced for successful development of novel drug delivery system.


Joshi V.D.,Mahatma Jyoti Rao Phoole University | Kshirsagar M.D.,P.A. College | Singhal S.,Mahatma Jyoti Rao Phoole University
Journal of Chemical and Pharmaceutical Research | Year: 2012

A new series of Isoxazole and Benzodizepine derivatives were synthesized from chalcones and evaluated for their Antimicrobial activities. First Chalcones were prepared by treatment of Furan-2-Carbaldehyde with different acetophenones by Claisen-Schimidt Condensation. Various Isoxazole derivatives were prepared by reaction of Chalcone with Hydroxylamine Hydrochloride and Sodium Acetate in ethanol and Benzodizepine derivatives were prepared by reaction of Chalcone in ethanol with o-phenylenediamine in presence of piperidine. The structures of the newly synthesized Isoxazole and Benzodiazepine derivatives have been established on the basis of their spectral data. The synthesized selected compounds were evaluated for their antimicrobial activities.


Yadav C.P.S.,Mahatma Jyoti Rao Phoole University
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: To explore the immunomodulatory properties of 80% ethanol extract and butanol fraction of Gentiana olivieri (G. olivieri) Griseb on Balb/C mice. Methods: The study wasperformed with basic models of immunomodulation such as the humoral antibody response(hemoglutination antibody titres), cell mediated immune response (delayed type hypersensitivity and in vivo carbon clearance or phagocytosis). Ethanol (80%) extract of flowering aerial parts of G. olivieri and its butanol fraction were administered p.o. (orally) to the mice. Levamisole, 2.5 mg/kg was used as standard drug. Results: There was a potentiation of immune response to sheep red bloodcells by cellular and humoral mediated mechanisms comparable to levamisole (2.5 mg/kg) by both 80% ethanol extract and the butanol fraction at doses of 50-200 mg/kg in male Balb/C mice. Both significantly (P<0.01) potentiated the humoral immune response in cyclophosphamide (250 mg/kg)immunosupressed mice at 100 and 200 mg/kg of each extract and fraction as compared to control. The potentiation of delayed type hypersensitivity response was statistically significant (P<0.01) at 200 mg/kg of ethanol extract and 100, 200 mg/kg of butanol fraction as compared to control. The phagocytosis was significant at 200 mg/kg with butanol fraction of G. olivieri. Conclusions: The results reveal the immunostimulant effects of plant G. olivieri in mice by acting through cellularand humoral immunity in experimental models of immunity in mice. Butanol fraction is the most effective at a dose level of 200 mg/kg.


Atul B.,Mahatma Jyoti Rao Phoole University
International Journal of Pharma and Bio Sciences | Year: 2012

The design and development of new drug delivery systems with the intention of enhancing the efficacy of existing drugs is an ongoing process in pharmaceutical research. It is necessary for a pharmaceutical solution to contain a therapeutic dose of the drug in a volume convenient for administration. In last decade, o/w micro/lipid emulsions have been recognized as an interesting and promising ocular topical delivery vehicle for lipophilic drugs. The aim of present review is to present the potential of o/w and lipidmicroemulsions for ocular delivery of lipophilic drugs. The review covers an update on the state of the art of incorporating the lipophilic drugs, a brief description concerning the components and the classification of lipid/oil in water emulsions. The ocular metabolism after topical instillation and the applications of micro/lipid emulsions are thoroughly discussed.


Bhaskar R.,Mahatma Jyoti Rao Phoole University | Sagar M.K.,Mahatma Jyoti Rao Phoole University | Saini V.,Mahatma Jyoti Rao Phoole University
Advanced Pharmaceutical Bulletin | Year: 2013

Purpose: In this work a numerical method, based on the use of spectrophotometric data coupled to partial least squares (PLS) regression and net analyte preprocessing combined with classical least square (NAP/CLS) multivariate calibration, is reported for the simultaneous determination of metformin hydrochloride (MET), gliclazide (GLZ) and pioglitazone hydrochloride (PIO) in synthetic samples and combined commercial tablets. Methods: Spectra of MET, GLZ and PIO were recorded at concentrations within their linear ranges (5-25 μg/ml, 0.5-8 μg/ml and 0.5-3 μg/ml respectively) and were used to compute a total of 25 synthetic mixtures involving 15 calibration and 10 validation sets between wavelength range of 200 and 400 nm in 0.1N HCl. The suitability of the models was decided on the basis of root mean square error (RMSE) values of calibration and validation data. Results: The analytical performances of these chemometric methods were characterized by relative prediction errors and recovery studies (%) and were compared with each other. These two methods were successfully applied to pharmaceutical formulation, tablet, with no interference with excipients as indicated by the recovery study results. Mean recoveries of the commercial formulation set together with the figures of merit (calibration sensitivity, selectivity, limit of detection, limit of quantification etc.) were estimated. Conclusion: The proposed methods are simple, rapid and can be easily used as an alternative analysis tool in the quality control of drugs and formulation. © 2013 by Tabriz University of Medical Sciences.


Chauhan D.,Mahatma Jyoti Rao Phoole University | Patel A.,Mahatma Jyoti Rao Phoole University | Shah S.,Mahatma Jyoti Rao Phoole University
International Journal of Drug Development and Research | Year: 2012

The objective of the present study was to develop and evaluate colon specific matrices of mebeverine HCl using various polysaccharides like guar gum, Locust bean gum and xanthan gum by direct compression method. The matrix tablets were evaluated for their physico-chemical properties, swelling study, in-vitro release study and stability studies. The prepared tablets were found to be uniform with respect to thickness (5.53 to 6.03 mm) and hardness (5.7 to 6.9 kg/cm2). The friability (0.41 to 0.95 %) and weight variation (1.04-1.66%) of different batch of tablets were found within prescribed limits. Drug content (96.01 to 99.89 %) was found uniform within the batches of different tablets. Swelling studies indicated that, matrix tablets prepared with XG (X4) swelled more as compared to those prepared using GG and LBG. Release profiles indicated that, increase in the polymer concentration has drastically retarded the release of Mebeverine Hcl. The optimized tablets prepared using GG (G4), LG (L4) & XG (X4) showed controlled release over periods of 24 hrs, whereas the marketed product controlled the drug release over a period of 12 hrs. The mechanism of drug release was Non-Fickian diffusion controlled first order kinetics for optimized matrix tablets of GG (G4) and LBG (L4) where as for XG (X4) it followed Highuchi model. The developed matrix tablets can be viewed as a better approach in the colonic delivery of Mebeverine HCl. © 2012 IJDDR, Dharmarajsinh Chauhan et al.


Chauhan D.,Mahatma Jyoti Rao Phoole University | Shah S.,Mahatma Jyoti Rao Phoole University
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

The objective of the present study was to design and evaluate time and pH dependent multiparticulate pulsatile delivery systems of Aceclofenac. Four batches of microspheres namely A1, A2, A3 and A4 were prepared using combination of Eudragit L-100 and S-100 with core: coat ratio 1:0.5, 1:1, 1:1.5 and 1:2 respectively by solvent evaporation technique. The drug content was uniform and reproducible in each batch of microspheres. FT-IR and DSC studies revealed the absence of drug polymer interactions. The release pattern of Aceclofenac from Eudragit microspheres of A1, A2, A3 and A4 batches were found 96.54, 93.71, 91.20 and 88.29% respectively at the end of 12 hrs. Pulsatile drug delivery system based on pulsincap® technology was designed using microspheres from formulation A3 as it showed better drug content, particle size, surface topography, in-vitro drug release and release kinetics. Different plugging materials like Sodium alginate, Locust bean gum and Psyllium husk were used in the design of pulsatile capsule. During dissolution studies the pulsatile capsule remained intact in acid buffer of pH 1.2 for 2 hrs due to enteric coat of the system with HPMCP. The enteric coat dissolved when the pH of medium was changed to 7.4. The pulsatile deliver system developed with Sodium alginate as plugging material showed satisfactory lag period when compared to locust bean gum and Psyllium husk.


PubMed | Mahatma Jyoti Rao Phoole University and Siksha ‘O’ Anusandhan University
Type: | Journal: Environmental science and pollution research international | Year: 2016

Screening of metagenomic library from Taptapani Hot Spring (Odisha) yielded a positive lipase clone (pUC-lip479). Sequence analysis showed an ORF (RK-lip479) of 416 amino acid residues which was overexpressed in Escherichia coli BL21 (DE3). Optimum pH and temperature of purified lipase RK-lip479 were 8.0 and 65C, respectively, and found to be stable over a pH range of 7.0-9.0 and temperatures 55-75C. RK-lip479 could hydrolyse a wide range of 4-nitrophenyl esters (4-nitrophenyoctanoate, 4-nitrophenyldodecanoate, 4-nitrophenylpalmitate, 4-nitrophenylmyristate and 4-nitrophenylstearate), and maximum activity was observed with 4-nitrophenyldodecanoate. RK-lip479 was resistant to many organic solvents, especially isopropanol, DMSO, methanol, DMF, ethanol, dichloromethane, acetone, glycerol and ethyl acetate. RK-lip479 also showed activity in the presence of monovalent (Na


Chauhan D.,Mahatma Jyoti Rao Phoole University | Jani R.,Mahatma Jyoti Rao Phoole University | Shah S.,Mahatma Jyoti Rao Phoole University
International Journal of Polymeric Materials and Polymeric Biomaterials | Year: 2013

The objective was to investigate the effect of different polysaccharide plugs on pulsatile device of Aceclofenac. The Aceclofenac microspheres were prepared in four batches, with Eudragit L-100 and S-100 (1:2) by varying drug to polymer ratio and evaluated for the particle size, drug content, and in vitro release profile and from the obtained results; one better formulation was selected for further fabrication of Pulsatile device. In vitro release mechanism of pulsatile device studied with different kinetics model. The X-ray study on humans pointed out the capability of the system to release drug in colon region. Copyright © 2013 Taylor & Francis Group, LLC.

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