Mahatma Jyoti Rao Phoole University
Jaipur, India

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Sahoo R.K.,Siksha ‘O’ Anusandhan University | Gaur M.,Siksha ‘O’ Anusandhan University | Das A.,Siksha ‘O’ Anusandhan University | Singh A.,Siksha ‘O’ Anusandhan University | And 2 more authors.
Geomicrobiology Journal | Year: 2017

Odisha (East India) is home to several hot springs, of which Atri and Taptapani are the two with variation in temperature and located in the Mahanadi geothermal province having altitude 120 and 1800 ft., respectively, above sea level. Average temperature of Atri hot spring is as higher as 58 ± 5°C as compared to 48 ± 5°C of Taptapani. In-depth analysis of the microbial diversity of these hot springs through 16S rRNA deep sequencing analysis, targeting V3 region was performed using Illumina bar-coded sequencing platform. Existence of higher microbial diversity in Atri hot spring (1662 OTUs; 2708 species) as compared to Taptapani (1561 Operational Taxonomic Units [OTUs]; 2045 species) is supported by higher value of diversity indices for Atri (6.24, Shannon; 0.95, Simpson) than Taptapani (4.03, Shannon; 0.79, Simpson), probably due to favorable influence of environmental parameters around it. Irrespective of the four databases (GREENGENE, M5RNA, Ribosomal Database Project [RDP], and Small Subunit [SSU] databases) used for understanding community structure, the dominant phyla in the Atri hot spring were different from the predominant populations in the Taptapani in terms of percentage representation in different databases. From Principal Coordinates Analysis [PCoA] analysis, Atri and Taptapani metagenome, on comparison with other three metagenomes, were found to be matching with the community structure of hot springs of Gujarat, India, but differed from that of saline desert. Furthermore, predicted functional analysis in both the hot springs were found to be affiliated with carbohydrate, amino acids, energy, vitamins and cofactor, nucleotide, membrane transport metabolic pathways, and the genes involved in them, although their intensity of occurrence was varying as analyzed through PICRUSt and Tax4Fun probably due to physicochemical parameters prevailing around each hot spring. The present study for the first time has revealed the differential microbial community structure and predicted functional diversity of Atri and Taptapani hot springs of Odisha in such a great detail. 2016 © 2017 Taylor & Francis Group, LLC

Yadav C.P.S.,Mahatma Jyoti Rao Phoole University
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: To explore the immunomodulatory properties of 80% ethanol extract and butanol fraction of Gentiana olivieri (G. olivieri) Griseb on Balb/C mice. Methods: The study wasperformed with basic models of immunomodulation such as the humoral antibody response(hemoglutination antibody titres), cell mediated immune response (delayed type hypersensitivity and in vivo carbon clearance or phagocytosis). Ethanol (80%) extract of flowering aerial parts of G. olivieri and its butanol fraction were administered p.o. (orally) to the mice. Levamisole, 2.5 mg/kg was used as standard drug. Results: There was a potentiation of immune response to sheep red bloodcells by cellular and humoral mediated mechanisms comparable to levamisole (2.5 mg/kg) by both 80% ethanol extract and the butanol fraction at doses of 50-200 mg/kg in male Balb/C mice. Both significantly (P<0.01) potentiated the humoral immune response in cyclophosphamide (250 mg/kg)immunosupressed mice at 100 and 200 mg/kg of each extract and fraction as compared to control. The potentiation of delayed type hypersensitivity response was statistically significant (P<0.01) at 200 mg/kg of ethanol extract and 100, 200 mg/kg of butanol fraction as compared to control. The phagocytosis was significant at 200 mg/kg with butanol fraction of G. olivieri. Conclusions: The results reveal the immunostimulant effects of plant G. olivieri in mice by acting through cellularand humoral immunity in experimental models of immunity in mice. Butanol fraction is the most effective at a dose level of 200 mg/kg.

Jain N.,Mahatma Jyoti Rao Phoole University | Singhal S.,Mahatma Jyoti Rao Phoole University | Kumar Jain S.,Sagar Institute of Research and Technology Pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2013

Objective: Stilbene based compounds are widely represented in nature and have become particular interest because of their wide range of biological activities. The aim of research work was to synthesize many synthetic compounds using stilbene as the essential pharmacophore. Methods: A series of novel P-(substituted phenyl) - 2 (substituted phenyl) Ethene derivatives (1a-1q) have been synthesized by the witting reactions i.e. Substituted benzyl chloride reacted with the phosphonium chloride salt give the P substituted benzyl (chloro) triphenyl phosporane (1) which on further reaction with substituted benzaldehyde yielded the corresponding 1a-1q. Results: The yield of synthesized compound was found to be in the range of 60-85%.Synthesized compounds were characterized by their physiochemical properties like solubility, melting point, IR spectroscopy, 1H NMR spectroscopy, Fab MASS and elemental analysis and result of analysis confirm the structure of synthesize compound. Conclusion: This procedure appears to be a promising and conceptually straightforward route for the synthesis of various Cis-stilbenes. The synthesis and characterization of other phenyl substituted compounds using this method are under investigation, and will be reported in due course.

Atul B.,Mahatma Jyoti Rao Phoole University
International Journal of Pharma and Bio Sciences | Year: 2012

The design and development of new drug delivery systems with the intention of enhancing the efficacy of existing drugs is an ongoing process in pharmaceutical research. It is necessary for a pharmaceutical solution to contain a therapeutic dose of the drug in a volume convenient for administration. In last decade, o/w micro/lipid emulsions have been recognized as an interesting and promising ocular topical delivery vehicle for lipophilic drugs. The aim of present review is to present the potential of o/w and lipidmicroemulsions for ocular delivery of lipophilic drugs. The review covers an update on the state of the art of incorporating the lipophilic drugs, a brief description concerning the components and the classification of lipid/oil in water emulsions. The ocular metabolism after topical instillation and the applications of micro/lipid emulsions are thoroughly discussed.

Bhaskar R.,Mahatma Jyoti Rao Phoole University | Sagar M.K.,Mahatma Jyoti Rao Phoole University | Saini V.,Mahatma Jyoti Rao Phoole University | Bhat K.,Manipal University India
Current Analytical Chemistry | Year: 2014

In this study, the simultaneous determination of verapamil hydrochloride, gliclazide and simvastatin in pharmaceuticals by chemometric approaches using UV spectrophotometry has been reported. Spectra of verapamil hydrochloride, gliclazide and simvastatin were recorded at several concentrations within their linear ranges and were used to compute the calibration mixture between wavelength range of 200 and 400 nm at an interval of 3 nm in pH 6.8 phosphate buffer containing 0.5% sodium lauryl sulphate (SLS). Partial least squares regression (PLS) and principle component regression (PCR) were used for chemometric analysis of the data. The analytical performances of these chemometric methods were characterized by relative prediction errors and recoveries (%) and were compared with each other. These two methods were successfully applied to pharmaceutical formulation, tablet, with no interference from excipients as indicated by the recovery study results. The proposed methods are simple, rapid and can be easily used as an alternative analysis tool in the quality control of drugs and formulation. © 2014 Bentham Science Publishers.

PubMed | Mahatma Jyoti Rao Phoole University and Siksha ‘O’ Anusandhan University
Type: | Journal: Environmental science and pollution research international | Year: 2016

Screening of metagenomic library from Taptapani Hot Spring (Odisha) yielded a positive lipase clone (pUC-lip479). Sequence analysis showed an ORF (RK-lip479) of 416 amino acid residues which was overexpressed in Escherichia coli BL21 (DE3). Optimum pH and temperature of purified lipase RK-lip479 were 8.0 and 65C, respectively, and found to be stable over a pH range of 7.0-9.0 and temperatures 55-75C. RK-lip479 could hydrolyse a wide range of 4-nitrophenyl esters (4-nitrophenyoctanoate, 4-nitrophenyldodecanoate, 4-nitrophenylpalmitate, 4-nitrophenylmyristate and 4-nitrophenylstearate), and maximum activity was observed with 4-nitrophenyldodecanoate. RK-lip479 was resistant to many organic solvents, especially isopropanol, DMSO, methanol, DMF, ethanol, dichloromethane, acetone, glycerol and ethyl acetate. RK-lip479 also showed activity in the presence of monovalent (Na

Chauhan D.,Mahatma Jyoti Rao Phoole University | Jani R.,Mahatma Jyoti Rao Phoole University | Shah S.,Mahatma Jyoti Rao Phoole University
International Journal of Polymeric Materials and Polymeric Biomaterials | Year: 2013

The objective was to investigate the effect of different polysaccharide plugs on pulsatile device of Aceclofenac. The Aceclofenac microspheres were prepared in four batches, with Eudragit L-100 and S-100 (1:2) by varying drug to polymer ratio and evaluated for the particle size, drug content, and in vitro release profile and from the obtained results; one better formulation was selected for further fabrication of Pulsatile device. In vitro release mechanism of pulsatile device studied with different kinetics model. The X-ray study on humans pointed out the capability of the system to release drug in colon region. Copyright © 2013 Taylor & Francis Group, LLC.

Sharma P.,Mahatma Jyoti Rao Phoole University | Sharma S.,Mahatma Jyoti Rao Phoole University
Australian Journal of Herbal Medicine | Year: 2013

Objective: A randomised, double-blind, placebo-controlled study was conducted to evaluate the efficacy of Aegle marmelos supplementation on blood sugar, glycosylated hemoglobin (HbA1C) and blood pressure levels in patients with type 2 diabetes mellitus. Method: After a one month stabilisation period, 150 patients with type 2 diabetes mellitus were randomly divided into 3 groups and given either 250mg/day or 600mg/day of Aegle marmelos or placebo. All groups were also given conventional treatment which consisted of diet and exercise for 3 months. Anthropometric parameters, blood pressure, fasting blood sugar (FBS) and HbA1C tests were performed initially and after 3 months of treatment. After 3 months decoding was done and data were statistically analysed by ANOVA and t-test. Results: At baseline the three groups were similar in anthropometric and clinical variables. After three months of supplementation there was a significant improvement in FBS and HbA1C in both active groups. In the 250mg/day group, FBS decreased from 12.60±4.01mmol/L to 9.73±4.99mmol/L (p<0.001) and HbA1Cdecreased from 8.84±0.11% to 7.02±0.11% (p<0.005). FBS reduced from 12.16±3.56mmol/L to 8.25±2.26mmol/L (p<0.001) and HbA1C from 8.60±0.13%to 6.69±0.11% (p<0.005) in the 600mg/day group. No significant difference was observed in BMI. There was also a significant decrease in systolic blood pressure in both active groups (132±1.56mmHg to 125.20±0.96mmHg (p<0.005) in the 250mg/day group and 136.4±1.89mmHg to 122.20±0.82mmHg (p<0.005) in the 600mg/day group). Aegle marmelos was tolerated by all patients and no adverse effects were observed in the study. Conclusion: In this study Aegle marmelos appears to be effective in controlling hyperglycaemia and blood pressure level of type 2 diabetes patients without any untoward effects. © National Herbalists Association of Australia 2013.

PubMed | Mahatma Jyoti Rao Phoole University
Type: Journal Article | Journal: Advanced pharmaceutical bulletin | Year: 2013

In this work a numerical method, based on the use of spectrophotometric data coupled to partial least squares (PLS) regression and net analyte preprocessing combined with classical least square (NAP/CLS) multivariate calibration, is reported for the simultaneous determination of metformin hydrochloride (MET), gliclazide (GLZ) and pioglitazone hydrochloride (PIO) in synthetic samples and combined commercial tablets.Spectra of MET, GLZ and PIO were recorded at concentrations within their linear ranges (5-25 g/ml, 0.5-8 g/ml and 0.5-3 g/ml respectively) and were used to compute a total of 25 synthetic mixtures involving 15 calibration and 10 validation sets between wavelength range of 200 and 400 nm in 0.1N HCl. The suitability of the models was decided on the basis of root mean square error (RMSE) values of calibration and validation data.The analytical performances of these chemometric methods were characterized by relative prediction errors and recovery studies (%) and were compared with each other. These two methods were successfully applied to pharmaceutical formulation, tablet, with no interference with excipients as indicated by the recovery study results. Mean recoveries of the commercial formulation set together with the figures of merit (calibration sensitivity, selectivity, limit of detection, limit of quantification etc.) were estimated.The proposed methods are simple, rapid and can be easily used as an alternative analysis tool in the quality control of drugs and formulation.

PubMed | Mahatma Jyoti Rao Phoole University
Type: Journal Article | Journal: Ayu | Year: 2012

Several methods exist for the treatment of cancer in modern medicine. These include chemotherapy, radiotherapy, and surgery; most cancer chemotherapeutants severely affect the host normal cells. Hence the use of natural products now has been contemplated of exceptional value in the control of cancer. Plant-derived natural products such as flavonoids, terpenes, alkaloids, etc., have received considerable attention in recent years due to their diverse pharmacological properties including cytotoxic and cancer chemopreventive effects. Looking into this, the antioxidant and anticancer evaluation of Scindapsus officinalis (Roxb.) Schott fruits has been attempted to investigate its antitumor activity. The collection and authentication of the plant material mainly fruits and their various extractions was done. Identification of plants active constituents by preliminary phytochemical screening was carried out. An in-vitro cytotoxic assay using the brine shrimp lethality assay with brine shrimp eggs (Artemia salina) at a dose of 1-10 g/ml with the fruit extract was performed by the method described by Mayer et al. Cell viability using the Trypan blue dye exclusion test at a dose of 20, 40, 80, 120, and 160 g/ml dissolved in DMSO (final concentration 0.1%), and cytotoxicity using the MTT assay where viable cells convert MTT into a formazan salt were performed. All pharmacological screening for acute toxicity and anti tumour studies using EAC 1 10(6) cells/mouse treated Swiss albino mice at a dose of 100 and 200 mg/kg/day orally was carried out. Biochemical and antioxidants predictions from various parameters like hematological, RBC, WBC count, PVC, total protein, Tissue Lipid Peroxidation, SOD, CATALASE, GPx, GST levels and anti tumour activity of Scindapsus officinalis were observed. The data was statistically analyzed by one-way ANOVA followed by Dunnetts and Tukeys multiple comparison test. The antitumor effect of the extract is evident from the increase in mean survival time (MST) lifespan, reduction in the solid tumor volume, and also the reversal of altered hematological parameters almost equal to normal. The methanolic extract (100-200 mg/kg/day orally) was found to be cytotoxic on human cancer cell lines. In addition, the methanolic extract had an antioxidant effect as reflected by a decrease in LPO, GST, and GPx (oxidant enzymes), and an increase in SOD and catalase.

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