Maharshi Dayanand University
Rohtak, India

Maharshi Dayanand University is a university in Rohtak, Haryana, India. It is accredited by NAAC. Established in 1976 and named after the saint Dayananda Saraswati, the university offers courses in a number of fields of study at the undergraduate, postgraduate and doctoral levels. Besides offering courses on campus, MDU is a collegiate university which provides programs through colleges, institutes and centers throughout the region. MDU Rohtak shares its campus with IIM Rohtak. Wikipedia.

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Sharma K.K.,Maharshi Dayanand University
Critical Reviews in Biotechnology | Year: 2015

The genome sequences provide a first glimpse into the genomic basis of the biological diversity of filamentous fungi and yeast. The genome sequence of the budding yeast, Saccharomyces cerevisiae, with a small genome size, unicellular growth, and rich history of genetic and molecular analyses was a milestone of early genomics in the 1990s. The subsequent completion of fission yeast, Schizosaccharomyces pombe and genetic model, Neurospora crassa initiated a revolution in the genomics of the fungal kingdom. In due course of time, a substantial number of fungal genomes have been sequenced and publicly released, representing the widest sampling of genomes from any eukaryotic kingdom. An ambitious genome-sequencing program provides a wealth of data on metabolic diversity within the fungal kingdom, thereby enhancing research into medical science, agriculture science, ecology, bioremediation, bioenergy, and the biotechnology industry. Fungal genomics have higher potential to positively affect human health, environmental health, and the planet’s stored energy. With a significant increase in sequenced fungal genomes, the known diversity of genes encoding organic acids, antibiotics, enzymes, and their pathways has increased exponentially. Currently, over a hundred fungal genome sequences are publicly available; however, no inclusive review has been published. This review is an initiative to address the significance of the fungal genome-sequencing program and provides the road map for basic and applied research. © 2015 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted

Sapna,Maharshi Dayanand University | Singh B.,Maharshi Dayanand University
Journal of Industrial Microbiology and Biotechnology | Year: 2013

Among three hundred isolates of filamentous fungi, Aspergillus oryzae SBS50 secreted higher phytase activity at pH 5.0, 35 C and 200 rpm after 96 h of fermentation. Starch and beef extract supported the highest phytase production than other carbon and nitrogen sources. A nine-fold improvement in phytase production was achieved due to optimization. Supplementation of the medium with inorganic phosphate repressed the enzyme synthesis. Among surfactants tested, Tween 80 increased fungal growth and phytase production, which further resulted in 5.4-fold enhancement in phytase production. The phytase activity was not much affected by proteases treatment. The enzyme resulted in the efficient hydrolysis of insoluble phytate complexes (metal- and protein-phytates) in a time dependent manner. Furthermore, the hydrolysis of insoluble phytates was also supported by scanning electron microscopy. The enzyme, being resistant to trypsin and pepsin, and able to hydrolyze insoluble phytates, can find an application in the animal food/feed industry for improving nutritional quality and also in combating environmental phosphorus pollution and plant growth promotion. © 2013 Society for Industrial Microbiology and Biotechnology.

Narang R.,Guru Jambheshwar University of Science and Technology | Narasimhan B.,Maharshi Dayanand University | Sharma S.,Guru Jambheshwar University of Science and Technology
Current Medicinal Chemistry | Year: 2012

There has been considerable interest in the development of novel compounds with anticonvulsant, antioxidant, hormone antagonist, analgesic, anti-inflammatory, antiplatelet, antimalarial, antimicrobial, antimycobacterial, antitumoral, vasodilator, antiviral and anti-trypanosomal activities. Hydrazones possessing an azometine -NHN=CH- proton constitute an important class of compounds for new drug development. Therefore, many researchers have synthesized these compounds as target structures and evaluated their biological activities. These observations have been guiding for the development of new hydrazide derivatives that possess varied biological activities. © 2012 Bentham Science Publishers.

As the world’s third largest carbon dioxide (CO2) emitter, India has long been believed to mostly compromise with international environmental obligation. Using annual data for the period 1970–2013, the study investigates the impact of population density, energy consumption, economic growth and trade openness on CO2 emissions in India. It applies the autoregressive distributed lag bounds testing approach to cointegration for establishing the existence of a long-run relationship and uses vector error correction model to determine the direction of causality between the variables. The results indicate that there is a meaningful long-run relationship between CO2 emissions and socioeconomic factors. We find that population density, energy consumption and economic growth have statistically significant positive effect on CO2 emissions both in the short-run and long-run. Among these three drivers, population density proves the main influencing factor of CO2 emissions changes. Therefore, a cautious population stabilization policy in the country would assist in reducing CO2 emissions and sustaining long-run economic growth. The findings further support the continued policy actions to develop the alternative energy sources such as renewable, and to use green and clean technologies to curb CO2 emissions without reducing energy consumption. © 2015, Springer Science+Business Media Dordrecht.

Chauhan N.,Maharshi Dayanand University | Pundir C.S.,Maharshi Dayanand University
Analytical Biochemistry | Year: 2011

An amperometric uric acid biosensor was fabricated by immobilizing uricase (EC onto gold nanoparticle (AuNP)/multiwalled carbon nanotube (MWCNT) layer deposited on Au electrode via carbodiimide linkage. Determination of uric acid was performed by oxidation of enzymically generated H 2O 2 at 0.4 V. The sensor showed optimal response within 7 s at 40 °C in 50 mM Tris-HCl buffer (pH 7.5). The linear working range of the biosensor was 0.01-0.8 mM. The limit of detection (LOD) was 0.01 mM. The sensor measured uric acid levels in serum of healthy individuals and persons suffering from gout. The analytical recoveries of the added uric acid, 10 and 20 mg L -1, were 98.0% and 96.5%, respectively. Within- and between-batch coefficients of variation were less than 5.6% and less than 4.7%, respectively. A good correlation (r = 0.998) was obtained between serum uric acid values by the standard enzymic colorimetric method and the current method. A number of serum substances had practically no interference. The sensor was used in more than 200 assays and had a storage life of 120 days at 4 °C. © 2011 Elsevier Inc. All rights reserved.

Singh B.,Maharshi Dayanand University
Critical Reviews in Biotechnology | Year: 2016

Myceliophthora thermophila syn. Sporotrichum thermophile is a ubiquitous thermophilic mould with a strong ability to degrade organic matter during optimal growth at 45 °C. Both genome analysis and experimental data have suggested that the mould is capable of hydrolyzing all major polysaccharides found in biomass. The mould is able to secrete a large number of hydrolytic enzymes (cellulases, laccases, xylanases, pectinases, lipases, phytases and some other miscellaneous enzymes) employed in various biotechnological applications. Characterization of the biomass-hydrolyzing activity of wild and recombinant enzymes suggests that this mould is highly efficient in biomass decomposition at both moderate and high temperatures. The native enzymes produced by the mould are more efficient in activity than their mesophilic counterparts beside their low enzyme titers. The mould is able to synthesize various biomolecules, which are used in multifarious applications. Genome sequence data of M. thermophila also supported the physiological data. This review describes the biotechnological potential of thermophilic mould, M. thermophila supported by genomic and experimental evidences. © 2014 Informa Healthcare USA, Inc.

Pundir C.S.,Maharshi Dayanand University | Chauhan N.,Maharshi Dayanand University
Analytical Biochemistry | Year: 2012

Pesticides released intentionally into the environment and through various processes contaminate the environment. Although pesticides are associated with many health hazards, there is a lack of monitoring of these contaminants. Traditional chromatographic methods - high-performance liquid chromatography, capillary electrophoresis, and mass spectrometry - are effective for the analysis of pesticides in the environment but have certain limitations such as complexity, time-consuming sample preparation, and the requirement of expensive apparatus and trained persons to operate. Over the past decades, acetylcholinesterase (AChE) inhibition-based biosensors have emerged as simple, rapid, and ultra-sensitive tools for pesticide analysis in environmental monitoring, food safety, and quality control. These biosensors have the potential to complement or replace the classical analytical methods by simplifying or eliminating sample preparation and making field-testing easier and faster with significant decrease in cost per analysis. This article reviews the recent developments in AChE inhibition-based biosensors, which include various immobilization methods, different strategies for biosensor construction, the advantages and roles of various matrices used, analytical performance, and application methods for constructing AChE biosensors. These AChE biosensors exhibited detection limits and linearity in the ranges of 1.0×10 -11 to 42.19 μM (detection limits) and 1.0 × 10 -11-1.0 × 10-2 to 74.5-9.9 × 103 μM (linearity). These biosensors were stable for a period of 2 to 120 days. The future prospects for the development of better AChE biosensing systems are also discussed. © 2012 Elsevier Inc. All rights reserved.

Commercial uricase was immobilized covalently onto iron oxide nanoparticles/chitosangraft-polyaniline (Fe3O4-NPs/CHIT-g- PANI) composite film electrodeposited on surface of Pt electrode. Transmission electron microscopy (TEM) was used for characterization of Fe3O 4-NPs. A uric acid biosensor was fabricated using/Fe 3O4-NPs/CHIT-g-PANI/Pt electrode as working electrode, Ag/AgCl as reference electrode and Pt wire as auxiliary electrode. The enzyme electrode was characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy and electrochemical impedance spectroscopy (EIS). The biosensor exhibited an optimum response within 1s at pH 7.5 and 30 C, when polarized at 0.4 V vs Ag/AgCl. The electrocatalytic response showed a linear dependence on uric acid concentration ranging from 0.1 to 800 μM. The sensitivity of the biosensor was 0.44 mA mM-1 cm-2, with a detection limit of 0.1 μM (S/N = 3). Apparent Michaelis-Menton (Km) value for uric acid was 12.5 μM, and Imax 0.008A. The biosensor showed only 10% loss in its initial response after 120 uses over 100 days, when stored at 4 C. The biosensor measured uric acid in the serum of apparently healthy persons, which correlated well with a standard enzymic colorimetric method (r = 0.98). © 2013 Published by Elsevier B.V.

Ohlan R.,Maharshi Dayanand University
South Asia Research | Year: 2016

India remains the world’s largest producer and consumer of milk. The present study empirically evaluates the transformations in demand and supply of dairy products in India, identifying that the share of dairy products in the food basket has significantly increased among both rural and urban consumers. While demand for dairy products is highly elastic and related to income, the analysis also shows large regional disparities in production and per capita availability of milk in India. After examining various aspects of dairy consumption and production patterns in India, the article also includes policy considerations to improve dairy production. It suggests that to stimulate dairy sector development and reduce inequality in dairy products consumption in India, milk and milk products should be included in the diet provided through the Midday Meal Schemes for primary education children. Overall, to meet the growing demand for dairy products, given their role in food security, the Government of India should continue to aim for sustained growth. © 2016, © 2016 SAGE Publications.

Maharshi Dayanand University | Date: 2011-12-07

A PVC surface co-immobilized with the multiple enzymes for removal of stains useful in the field of washing or cleaning cloth and other household textile such as towels and sheets. The present invention also provides a process of preparation of the PVC surfaces and using such PVC surface. The PVC surface co-immobilized with the enzymes is useful as cheap and reusable alternative for washing of cloths.

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