Maharshi Dayanand University
Rohtak, India

Maharshi Dayanand University is a university in Rohtak, Haryana, India. It is accredited by NAAC. Established in 1976 and named after the saint Dayananda Saraswati, the university offers courses in a number of fields of study at the undergraduate, postgraduate and doctoral levels. Besides offering courses on campus, MDU is a collegiate university which provides programs through colleges, institutes and centers throughout the region. MDU Rohtak shares its campus with IIM Rohtak. Wikipedia.

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Prakash C.,Maharshi Dayanand University | Soni M.,Maharshi Dayanand University | Kumar V.,Maharshi Dayanand University
Journal of Applied Toxicology | Year: 2016

Arsenic is a toxic metalloid present ubiquitously on earth. Since the last decade, it has gained considerable attention due to its severe neurotoxic effects. Arsenic can cross the blood-brain barrier and accumulate in different regions of the brain suggesting its role in neurological diseases. Arsenic exposure has been associated with reactive oxygen species generation, which is supposed to be one of the mechanisms of arsenic-induced oxidative stress. Mitochondria, being the major source of reactive oxygen species generation may present an important target of arsenic toxicity. It is speculated that the proper functioning of the brain depends largely on efficient mitochondrial functions. Multiple studies have reported evidence of brain mitochondrial impairment after arsenic exposure. In this review, we have evaluated the proposed mechanisms of arsenic-induced mitochondrial oxidative stress and dysfunction. The understanding of molecular mechanism of mitochondrial dysfunction may be helpful to develop therapeutic strategies against arsenic-induced neurotoxicity. The ameliorative measures undertaken in arsenic-induced mitochondrial dysfunction have also been highlighted. © 2016 John Wiley & Sons, Ltd.

Chhillar A.K.,Maharshi Dayanand University
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

Objective: Antimicrobial potential of ten medicinal plants i.e. Picrorhiza kurroa, Datura metel, Acacia catechu, Cissus quadrangularis, Cassia tora, Berberis aristata, Pongamia pinnata, Emblica officinalis, Saraca asoca and Tinospora cordifolia was evaluated against six bacterial strains i.e. Bacillus subtilis, Staphylococcus aureus, Salmonella typhi, Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa. Methods: Plant extracts were prepared by using Soxhlet extraction. Five extract from each plant were prepared using five solvents on the basis of increasing polarity. Minimum inhibitory concentration of extracts was determined by resazurin based microtiter dilution assay. Results: Percent yields of petroleum ether and chloroform extracts of plant leaves was found to be in the range of 0.80 - 2.98%. Percent yields of acetone, methanol and water extract were found to ranging from 2.87- 5.77%. S. asoca extracts were found to be endowed with highest antimicrobial activity out of the ten plants used in the study. Leaves water extract of S. asoca showed lowest minimum inhibitory concentration (0.15 mg/mL) against Pseudomonas aeruginosa. Conclusion: It was observed that leave water extracts of S. asoca could be potential reservoir of bioactive compounds. Post treatment analysis of proteome of test micro-organisms could explore potential anti-bacterial targets.

Kumar V.,Maharshi Dayanand University | Gill K.D.,Maharshi Dayanand University | Gill K.D.,Jawaharlal Institute of Postgraduate Medical Education & Research
NeuroToxicology | Year: 2014

Aluminium is light weight and toxic metal present ubiquitously on earth which has gained considerable attention due to its neurotoxic effects. The widespread use of products made from or containing aluminium is ensuring its presence in our body. There is prolonged retention of a fraction of aluminium that enters the brain, suggesting its potential for accumulation with repeated exposures. There is no known biological role for aluminium within the body but adverse physiological effects of this metal have been observed in mammals. The generation of oxidative stress may be attributed to its toxic consequences in animals and humans. The oxidative stress has been implicated in pathogenesis of various neurodegenerative conditions including Alzheimer's disease and Parkinson's disease. Though it remains unclear whether oxidative stress is a major cause or merely a consequence of cellular dysfunction associated with neurodegenerative diseases, an accumulating body of evidence implicates that impaired mitochondrial energy production and increased mitochondrial oxidative damage is associated with the pathogenesis of neurodegenerative disorders. Being involved in the production of reactive oxygen species, aluminium may impair mitochondrial bioenergetics and may lead to the generation of oxidative stress. In this review, we have discussed the oxidative stress and mitochondrial dysfunctions occurring in Al neurotoxicity. In addition, the ameliorative measures undertaken in aluminium induced oxidative stress and mitochondrial dysfunctions have also been highlighted. © 2014 Elsevier Inc.

Kumar P.,Maharshi Dayanand University | Narasimhan B.,Maharshi Dayanand University
Mini-Reviews in Medicinal Chemistry | Year: 2013

Hydrazide/hydrazone derivatives are of quite interest for medicinal chemists because of their vast spectrum of biological activity. Literature reports reveal that the hydrazide derivatives have been extensively studied for their biological profile during the past decade. The aim of the present work is to collect literature reports focusing the antimicrobial and anticancer study of different hydrazide/hydrazone derivatives carried out during the past decade which will serve as a valuable source of information for the researchers working in the field of antimicrobial and anticancer research. © 2013 Bentham Science Publishers.

Sharma K.K.,Maharshi Dayanand University
Critical Reviews in Biotechnology | Year: 2015

The genome sequences provide a first glimpse into the genomic basis of the biological diversity of filamentous fungi and yeast. The genome sequence of the budding yeast, Saccharomyces cerevisiae, with a small genome size, unicellular growth, and rich history of genetic and molecular analyses was a milestone of early genomics in the 1990s. The subsequent completion of fission yeast, Schizosaccharomyces pombe and genetic model, Neurospora crassa initiated a revolution in the genomics of the fungal kingdom. In due course of time, a substantial number of fungal genomes have been sequenced and publicly released, representing the widest sampling of genomes from any eukaryotic kingdom. An ambitious genome-sequencing program provides a wealth of data on metabolic diversity within the fungal kingdom, thereby enhancing research into medical science, agriculture science, ecology, bioremediation, bioenergy, and the biotechnology industry. Fungal genomics have higher potential to positively affect human health, environmental health, and the planet’s stored energy. With a significant increase in sequenced fungal genomes, the known diversity of genes encoding organic acids, antibiotics, enzymes, and their pathways has increased exponentially. Currently, over a hundred fungal genome sequences are publicly available; however, no inclusive review has been published. This review is an initiative to address the significance of the fungal genome-sequencing program and provides the road map for basic and applied research. © 2015 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted

As the world’s third largest carbon dioxide (CO2) emitter, India has long been believed to mostly compromise with international environmental obligation. Using annual data for the period 1970–2013, the study investigates the impact of population density, energy consumption, economic growth and trade openness on CO2 emissions in India. It applies the autoregressive distributed lag bounds testing approach to cointegration for establishing the existence of a long-run relationship and uses vector error correction model to determine the direction of causality between the variables. The results indicate that there is a meaningful long-run relationship between CO2 emissions and socioeconomic factors. We find that population density, energy consumption and economic growth have statistically significant positive effect on CO2 emissions both in the short-run and long-run. Among these three drivers, population density proves the main influencing factor of CO2 emissions changes. Therefore, a cautious population stabilization policy in the country would assist in reducing CO2 emissions and sustaining long-run economic growth. The findings further support the continued policy actions to develop the alternative energy sources such as renewable, and to use green and clean technologies to curb CO2 emissions without reducing energy consumption. © 2015, Springer Science+Business Media Dordrecht.

Chauhan N.,Maharshi Dayanand University | Pundir C.S.,Maharshi Dayanand University
Analytical Biochemistry | Year: 2011

An amperometric uric acid biosensor was fabricated by immobilizing uricase (EC onto gold nanoparticle (AuNP)/multiwalled carbon nanotube (MWCNT) layer deposited on Au electrode via carbodiimide linkage. Determination of uric acid was performed by oxidation of enzymically generated H 2O 2 at 0.4 V. The sensor showed optimal response within 7 s at 40 °C in 50 mM Tris-HCl buffer (pH 7.5). The linear working range of the biosensor was 0.01-0.8 mM. The limit of detection (LOD) was 0.01 mM. The sensor measured uric acid levels in serum of healthy individuals and persons suffering from gout. The analytical recoveries of the added uric acid, 10 and 20 mg L -1, were 98.0% and 96.5%, respectively. Within- and between-batch coefficients of variation were less than 5.6% and less than 4.7%, respectively. A good correlation (r = 0.998) was obtained between serum uric acid values by the standard enzymic colorimetric method and the current method. A number of serum substances had practically no interference. The sensor was used in more than 200 assays and had a storage life of 120 days at 4 °C. © 2011 Elsevier Inc. All rights reserved.

Pundir C.S.,Maharshi Dayanand University | Chauhan N.,Maharshi Dayanand University
Analytical Biochemistry | Year: 2012

Pesticides released intentionally into the environment and through various processes contaminate the environment. Although pesticides are associated with many health hazards, there is a lack of monitoring of these contaminants. Traditional chromatographic methods - high-performance liquid chromatography, capillary electrophoresis, and mass spectrometry - are effective for the analysis of pesticides in the environment but have certain limitations such as complexity, time-consuming sample preparation, and the requirement of expensive apparatus and trained persons to operate. Over the past decades, acetylcholinesterase (AChE) inhibition-based biosensors have emerged as simple, rapid, and ultra-sensitive tools for pesticide analysis in environmental monitoring, food safety, and quality control. These biosensors have the potential to complement or replace the classical analytical methods by simplifying or eliminating sample preparation and making field-testing easier and faster with significant decrease in cost per analysis. This article reviews the recent developments in AChE inhibition-based biosensors, which include various immobilization methods, different strategies for biosensor construction, the advantages and roles of various matrices used, analytical performance, and application methods for constructing AChE biosensors. These AChE biosensors exhibited detection limits and linearity in the ranges of 1.0×10 -11 to 42.19 μM (detection limits) and 1.0 × 10 -11-1.0 × 10-2 to 74.5-9.9 × 103 μM (linearity). These biosensors were stable for a period of 2 to 120 days. The future prospects for the development of better AChE biosensing systems are also discussed. © 2012 Elsevier Inc. All rights reserved.

Commercial uricase was immobilized covalently onto iron oxide nanoparticles/chitosangraft-polyaniline (Fe3O4-NPs/CHIT-g- PANI) composite film electrodeposited on surface of Pt electrode. Transmission electron microscopy (TEM) was used for characterization of Fe3O 4-NPs. A uric acid biosensor was fabricated using/Fe 3O4-NPs/CHIT-g-PANI/Pt electrode as working electrode, Ag/AgCl as reference electrode and Pt wire as auxiliary electrode. The enzyme electrode was characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy and electrochemical impedance spectroscopy (EIS). The biosensor exhibited an optimum response within 1s at pH 7.5 and 30 C, when polarized at 0.4 V vs Ag/AgCl. The electrocatalytic response showed a linear dependence on uric acid concentration ranging from 0.1 to 800 μM. The sensitivity of the biosensor was 0.44 mA mM-1 cm-2, with a detection limit of 0.1 μM (S/N = 3). Apparent Michaelis-Menton (Km) value for uric acid was 12.5 μM, and Imax 0.008A. The biosensor showed only 10% loss in its initial response after 120 uses over 100 days, when stored at 4 C. The biosensor measured uric acid in the serum of apparently healthy persons, which correlated well with a standard enzymic colorimetric method (r = 0.98). © 2013 Published by Elsevier B.V.

Maharshi Dayanand University | Date: 2011-12-07

A PVC surface co-immobilized with the multiple enzymes for removal of stains useful in the field of washing or cleaning cloth and other household textile such as towels and sheets. The present invention also provides a process of preparation of the PVC surfaces and using such PVC surface. The PVC surface co-immobilized with the enzymes is useful as cheap and reusable alternative for washing of cloths.

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