Maharashtra Institute of Medical science and Research

Latur, India

Maharashtra Institute of Medical science and Research

Latur, India
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Suryawanshi N.,Maharashtra Institute of Medical science and Research | Nagoba B.,Maharashtra Institute of Medical science and Research
European Journal of General Medicine | Year: 2016

The human oral cavity is the highly selective environment, which can be colonized by certain microbes. Viridans group streptococci are the most common bacterial isolates along with many other aerobic and anaerobic bacteria. S. salivarius is the most common viridians group streptococcus followed by S. mitis, S. sanguis, S. milleri, S. gordonii and S. mutans. The diverse habitat unique to the oral cavity together with a variety of nutrients is supportive to mixed population. These members of the resident oral flora, including viridians group streptococci, are responsible for colonization resistance, i.e., they prevent colonization/invasion by more pathogenic exogenous bacterial pathogens. Disruption of the oral ecosystem may lead to impairment of colonization resistance leading to higher rates of colonization by more pathogenic exogenous bacterial pathogens. Thus, viridians group streptococci normally present in the oral cavity play a significant role in oral health. © 2016, TIP ARASTIRMALARI DERNEGI. All rights reserved.


Dhotre S.,Ashwini Medical College and Hospital | Suryawanshi N.,Maharashtra Institute of Medical science and Research | Nagoba B.,Maharashtra Institute of Medical science and Research
Indian Journal of Pathology and Microbiology | Year: 2016

Context: The genus Streptococcus consists of more than 65 species. The taxonomic classification of these members is not well-defined. Among the viridans group streptococci (VGS), there are major taxonomic changes by the addition of many new species; whereas, most of the new strains are of animal origin and only a few have been reported to be isolated from humans. Rare and unusual species of VGS such as Streptococcus thoraltensis, S. pluranimalium and S. hyointestinalis are normally associated with different animals. Their isolation from human being is not yet reported. Aim: To find out the rare and unusual species of viridans group streptococci from human oral cavity. Settings and Design: A case-controlled study carried out at hospital-based dental services in a tertiary care hospital. Materials and Methods: Subgingival plaque samples of the tooth were collected from 80 patients (34 with periodontitis and 46 without periodontitis) undergoing tooth extraction. Cultures were subcultured onto special media such as Tryptone Soya blood Agar supplemented with strepto supplement and Mutans-Sanguis Agar. Identification of strains and antimicrobial susceptibilities were measured as minimum inhibitory concentration using Vitek 2 (BioMérieux, Paris, France) automated system. Results: We have identified three strains of VGS - S. thoraltensis, S. pluranimalium and S. hyointestinalis from subgingival plaque samples from patients with periodontitis. S. thoraltensis and S. pluranimalium were found to be resistant to most of the antibiotics. Conclusions: To the best of our knowledge, this is the first report of isolation of these rare and unusual strains from the human oral cavity. © 2016 Indian Journal of Pathology and Microbiology.


Rao A.,Maharashtra Institute of Medical science and Research | Ingle S.B.,Review-Board | Rajurkar P.,Maharashtra Institute of Medical science and Research | Goyal V.,Maharashtra Institute of Medical science and Research | Dokrimare N.,Maharashtra Institute of Medical science and Research
World Journal of Clinical Oncology | Year: 2015

A 16-year-old man had aswelling over the anterior aspect of the proximal third of the tibia for 1 year, which was peanut size initially and progressively increased to its present size of 10 cm × 8 cm. He underwent fine needle aspiration cytology (FNAC) twice during this period and reported aspindle cell sarcoma. Malignant peripheral nerve sheath tumor (MPNST) is a malignancy of the connective tissue surrounding the nerves. Previously, MPNST was also known as neurofibrosarcoma, malignant schwannoma, andneurogenic sarcoma. We are reporting this case for its rarity and peculiar mode of presentation. FNAC/core biopsy can be used as an effective tool to achievethe correct pathological diagnosis. © 2015 Baishideng Publishing Group Inc. All rights reserved.


Dhotre S.V.,Hospital and Research Center | Mehetre G.T.,CSIR - National Chemical Laboratory | Dharne M.S.,CSIR - National Chemical Laboratory | Suryawanshi N.M.,Maharashtra Institute of Medical science and Research | Nagoba B.S.,Maharashtra Institute of Medical science and Research
FEMS Microbiology Letters | Year: 2014

Streptococcus tigurinus is a new member of the Streptococcus viridians group and is closely related to Streptococcus mitis, Streptococcus pneumoniae, Streptococcus pseudopneumoniae, Streptococcus oralis, and Streptococcus infantis. The type strain AZ_3aT of S. tigurinus was originally isolated from a patient with infective endocarditis. Accurate identification of S. tigurinus is facilitated only by newer molecular methods like 16S rRNA gene analysis. During the course of study on bacteraemia and infective endocarditis with reference to periodontitis and viridians group of streptococci, a strain of S. tigurinus isolated from subgingival plaque of a patient with periodontitis identified by 16S rRNA gene analysis, which was originally identified as Streptococcus pluranimalium by Vitek 2. Confirmation by 16S rRNA gene analysis showed 99.39% similarity (1476/1485 bp) with S. tigurinus AZ_3aT(AORU01000002). To the best of our knowledge, this is the first report of isolation of S. tigurinus from the oral cavity of a periodontitis patient. © 2014 Federation of European Microbiological Societies.


Nagoba B.S.,Maharashtra Institute of Medical science and Research | Vedpathak D.V.,Rajarshi Shahu College | Selkar S.P.,Maharashtra Institute of Physiotherapy | Suryawanshi N.M.,Maharashtra Institute of Medical science and Research
Journal of Krishna Institute of Medical Sciences University | Year: 2015

Bone-marrow derived stem cells are multi potential or totipotent and are able to differentiate into numerous cell types. Their application is indicated in various reconstructive and restorative surgeries for rapid healing. A technique for creating cells that have the embryonic ability to turn into almost any cell type in the mammalian body has been reported. Recently, an unexpected phenomenon of somatic cell reprogramming into pluripotent cells by exposing to sublethal stimuli such as citrate based acidic medium has been reported. With the concept of creating acidic environment in chronic infected wounds to make a condition unsuitable for growth and multiplication of bacteria using 3% citric acid has been reported. It would be interesting to study whether the phenomenon of pluripotency takes place in chronic infected wounds in human beings following the application of 3% citric acid and plays an important role in formation of healthy granulation tissue. © Journal of Krishna Institute of Medical Sciences University.


Mahaling D.U.,Government Medical College | Basavaraj M.M.,Maharashtra Institute of Medical Science and Research | Bika A.J.,Government Medical College
Asian Pacific Journal of Tropical Biomedicine | Year: 2013

Objective: To detect and compare serum lipid abnormalities in patients diagnosed with different grades of non-alcoholic fatty liver on ultrasonography. Methods: A total of 70 cases which included 30 males and 40 females, diagnosed as non-alcoholic fatty liver disease (NAFLD) on ultrasound were investigated with serum lipid profile. Then a comparison of lipid abnormalities between different grades of fatty liver diagnosed on ultrasound was done. P value was calculated by using analysis of variance test (ANOVA) and P value <0.05 was considered as statistically significant. Results: Out of 70 cases which were diagnosed as NAFLD on ultrasonography, grade I NAFLD cases were 47.15%, grade II were 42.85% and grade III were 10%. The mean age of the patients was 49.14 years. Male to female ratio was 3:4. Serum triglycerides, total cholesterol, LDL and VLDL levels were raised in 67.14%, 45.71% 34.28%, 25.71% of cases respectively. Low serum HDL levels were seen in 62.85% of patients. On statistical analysis we found increasing grades of NAFLD were significantly associated with increasing values of total cholesterol (P value-0.001), LDL (P value-0.000) and VLDL (P value-0.003) and decreasing HDL (P value-0.000). Conclusion: Most of the patients of NAFLD in India is asymptomatic, non-diabetic and non-hypertensive. Though liver biopsy is the gold standard method for diagnosis of NAFLD, Ultrasonography which is non-invasive, simple tool, can be used for the early detection of NAFLD in asymptomatic patients. © 2013 Asian Pacific Tropical Biomedical Magazine.


Vishnudas B.,Maharashtra Institute of Dental science and Research | Sameer Z.,Maharashtra Institute of Dental science and Research | Shriram B.,Maharashtra Institute of Dental science and Research | Rekha K.,Maharashtra Institute of Medical Science and Research
Journal of Natural Science, Biology and Medicine | Year: 2014

Drug-induced gingival overgrowth (DIGO) can be a serious concern for both patients and clinicians. DIGO is a well-documented side-effect of some pharmacologic agents, including, but not limited to, calcium channel blockers, phenytoin, and cyclosporine. Plasma cell granulomas (pseudotumors) are exceedingly rare, non-neoplastic, reactive tumor-like proliferation, primarily composed of plasma cells that manifest primarily in the lungs, but may occur in various anatomic locations. Intraoral plasma cell granulomas involving the lip, oral mucosa, tongue, and gingiva have been reported in the past. This is the first case report of amlodipine induced plasma cell granuloma of the gingiva in the medical literature presenting a 54 year-old female patient with hypertension, who received amlodipine (10 mg/day, single dose orally) for 2 years, sought medical attention because of developing maxillary anterior massive gingival overgrowth causing functional and esthetic problem, which was treated by excisional biopsy. Histologically, these lesions were composed of mature plasma cells, showing polyclonality for both lambda and kappa light chains and fibrovascular connective tissue stroma confirming a diagnosis of plasma cell granuloma. This case also highlights the need to biopsy for unusual lesions to rule out potential neoplasms. © Journal of Natural Science, Biology and Medicine.


Ganesh M.,Dr Vm Medical College | Sheikh N.K.,Dr Vm Medical College | Shah P.,Dr Vm Medical College | Mehetre G.,CSIR - National Chemical Laboratory | And 2 more authors.
Journal of Infection and Public Health | Year: 2016

Tetanus resulting from ear injury remains an important health problem, particularly in the developing world. We report the successful detection of Clostridium tetani using tetX specific primers targeting the Cl. tetani neurotoxin. The sample was obtained from an ear discharge of a case of otogenic tetanus in a 2-year-old male child. Based on the culture results of the ear discharge, Gram staining and virulence testing by genotyping, a diagnosis of tetanus was confirmed. This is the first report from India on the successful detection of Cl. tetani in a human clinical sample using tetX specific primers targeting the Cl. tetani neurotoxin. © 2015 King Saud Bin Abdulaziz University for Health Sciences.


PubMed | Maharashtra Institute of Medical science and Research
Type: Journal Article | Journal: World journal of clinical oncology | Year: 2015

A 16-year-old man had aswelling over the anterior aspect of the proximal third of the tibia for 1 year, which was peanut size initially and progressively increased to its present size of 10 cm 8 cm. He underwent fine needle aspiration cytology (FNAC) twice during this period and reported aspindle cell sarcoma. Malignant peripheral nerve sheath tumor (MPNST) is a malignancy of the connective tissue surrounding the nerves. Previously, MPNST was also known as neurofibrosarcoma, malignant schwannoma, andneurogenic sarcoma. We are reporting this case for its rarity and peculiar mode of presentation. FNAC/core biopsy can be used as an effective tool to achievethe correct pathological diagnosis.


PubMed | Maharashtra Institute of Medical science and Research
Type: Journal Article | Journal: Indian journal of pathology & microbiology | Year: 2016

The genus Streptococcus consists of more than 65 species. The taxonomic classification of these members is not well-defined. Among the viridans group streptococci (VGS), there are major taxonomic changes by the addition of many new species; whereas, most of the new strains are of animal origin and only a few have been reported to be isolated from humans. Rare and unusual species of VGS such as Streptococcus thoraltensis, S. pluranimalium and S. hyointestinalis are normally associated with different animals. Their isolation from human being is not yet reported.To find out the rare and unusual species of viridans group streptococci from human oral cavity.A case-controlled study carried out at hospital-based dental services in a tertiary care hospital.Subgingival plaque samples of the tooth were collected from 80 patients (34 with periodontitis and 46 without periodontitis) undergoing tooth extraction. Cultures were subcultured onto special media such as Tryptone Soya blood Agar supplemented with strepto supplement and Mutans-Sanguis Agar. Identification of strains and antimicrobial susceptibilities were measured as minimum inhibitory concentration using Vitek 2 (BioMrieux, Paris, France) automated system.We have identified three strains of VGS - S. thoraltensis, S. pluranimalium and S. hyointestinalis from subgingival plaque samples from patients with periodontitis. S. thoraltensis and S. pluranimalium were found to be resistant to most of the antibiotics.To the best of our knowledge, this is the first report of isolation of these rare and unusual strains from the human oral cavity.

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