Maharashtra Hybrid Seeds Company Ltd
Maharashtra Hybrid Seeds Company Ltd
Panchbhai A.,Maharashtra Hybrid Seeds Co. |
Panchbhai A.,Dr. Babasaheb Ambedkar Marathwada University |
Char B.,Maharashtra Hybrid Seeds Co. |
Kharat A.S.,Dr. Babasaheb Ambedkar Marathwada University
Plant Gene | Year: 2017
The most popular CaMV 35S promoter used for the construction of transgenic plants is a constitutive promoter. The use of conditionally expressed promoters would be a better option as the energy towards expression would be accounted for when the need for expression is indicated. The Acacia nilotica has been known to sustain severe moisture stress. The ALDH7 gene has been reported to be an antiquitin. The PCR amplicon for the ALDH7 promoter from A. nilotica was obtained and sequenced from both the ends to decipher nucleotide sequence. Nucleotide sequence analysis of the A. nilotica ALDH7 promoter indicated the presence of a response element. The nucleotide analysis using PLACE indicated presence of the ACGT, drought and salinity stress response element whereas NNPP indicated existence of seven transcriptional start sites and TSSP indicated presence of two promoters. The PCR amplified ALDH7 promoter of A. nilotica was cloned into a binary vector carrying a promoter-less GUS cassette. Thus, the GUS gene could be expressed under the influence of the A. nilotica ALDH7 promoter. Transgenic tobacco plants expressing the GUS gene under the A. nilotica ALDH7 promoter were constructed. Using histochemical staining, the GUS gene expression levels in transgenic tobacco plants during moisture stress and under irrigation were studied. Histochemical staining experiments demonstrated that the GUS gene in transgenic plants was expressed only during moisture stress and remained unexpressed while irrigation was maintained. In contrast, transgenic tobacco plants expressing the GUS gene under the CaMV 35S promoter were found to be constitutive. The histochemical studies also indicated that the GUS expression was stable at least for two generations. Studies of the gene indicated that transgene segregation was in agreement with that of a monogenic trait described by Mendelian inheritance. The ALDH7 promoter of A. nilotica is a conditionally expressible promoter, expressed during moisture stress and suppressed during irrigation. © 2017
Rajagopalan P.A.,Maharashtra Hybrid Seeds Company Ltd |
Naik A.,Maharashtra Hybrid Seeds Company Ltd |
Katturi P.,Maharashtra Hybrid Seeds Company Ltd |
Kurulekar M.,Maharashtra Hybrid Seeds Company Ltd |
And 2 more authors.
Archives of Virology | Year: 2012
Cotton leaf curl disease (CLCuD) is a major limitation to cotton production on the Indian subcontinent. A survey for viruses causing CLCuD was conducted during the 2009 and 2010 cropping seasons in the northwestern Indian cotton-growing belt in the states of Punjab, Haryana and Rajasthan. Partial sequences of 258 and full-length sequences of 22 virus genomes were determined. This study shows that the resistance-breaking cotton leaf curl Burewala virus (CLCuBuV) is now the dominant virus in many fields. The spread and establishment of the mutant CLCuBuV in northwestern India, the variation in its genomic sequence, its virulence and infectivity, and the implications for cotton breeding are discussed. © 2012 Springer-Verlag.
Bhattacharya A.,Maharashtra Hybrid Seeds Company Ltd |
Khanale V.,Maharashtra Hybrid Seeds Company Ltd |
Char B.,Maharashtra Hybrid Seeds Company Ltd
Indian Journal of Plant Physiology | Year: 2017
An endogenous regulatory mechanism is required for plants to face diurnal environmental changes as a compensation for their sessile nature. The circadian clock helps plants to anticipate diurnal changes. Many physiological processes in plants like stress acclimatization, hormone signaling, photo morphogenesis, carbon metabolism and defense signaling are currently being explored for their cross linking with the circadian clock. The hypothesis of involvement of the circadian clock in the maintenance of plant homeostasis against these changes was proposed a few decades ago. Several lines of evidence including, phenotypic, interactomic, genomic and metabolomic studies of clock defective mutants strengthened this hypothesis and favored the critical role of the circadian clock in stress acclimatization. Conserved regulatory elements like circadian clock factors, light responsive motifs in the regulatory regions of stress responsive genes and cis-regulatory elements like ABRE and DRE in the clock gene promoters, indicate the involvement of circadian clock and light regulators in the transcriptional regulation of stress response genes and vice versa. Presented here, are the highlights of recent advancements in understanding of the clock–stress physiology in plants. This review examines the experimental evidences, collected over the past few years, to relate circadian clock with abiotic stress tolerance in plants. Insights of this dynamic relation between the clock and stress will improve our current understanding of the multifaceted circadian clock and endogenous mechanism of stress adaptation in plants. © 2017, Indian Society for Plant Physiology.
Baranwal V.K.,University of Delhi |
Mikkilineni V.,Maharashtra Hybrid Seeds Company Ltd |
Zehr U.B.,Maharashtra Hybrid Seeds Company Ltd |
Tyagi A.K.,National Institute of Plant Genome Research |
Kapoor S.,University of Delhi
Journal of Experimental Botany | Year: 2012
Perceived by Charles Darwin in many vegetable plants and rediscovered by George H Shull and Edward M East in maize, heterosis or hybrid vigour is one of the most widely utilized phenomena, not only in agriculture but also in animal breeding. Although, numerous studies have been carried out to understand its genetic and/or molecular basis in the past 100 years, our knowledge of the underlying molecular processes that results in hybrid vigour can best be defined as superficial. Even after century long deliberations, there is no consensus on the relative/individual contribution of the genetic/epigenetic factors in the manifestation of heterosis. However, with the recent advancements in functional genomics, transcriptomics, proteomics, and metabolomics-related technologies, the riddle of heterosis is being reinvestigated by adopting systems-level approaches to understand the underlying molecular mechanisms. A number of intriguing hypotheses are converging towards the idea of a cumulative positive effect of the differential expression of a variety of genes, on one or several yield-affecting metabolic pathways or overall energy-use efficiency, as the underlying mechanism for the manifestation of heterosis. Presented here is a brief account of clues gathered from various investigative approaches targeted towards better scientific understanding of this process. © 2012 The Author . Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please email: firstname.lastname@example.org.
MAHARASHTRA HYBRID SEEDS Co. | Date: 2011-09-13
The present description concerns methods for regeneration of whole plant from the explants obtained from the Abelmoschus species preferably A. esculentus. In addition the present description also concerns methods for transforming okra plant, plant cells and tissues either with the use of recombinant Agrobacterium strain or by bombarding the explants with tungsten or gold particles coated with DNA sequences of interest. An efficient method to isolate embryos from imbibed seeds of okra is also described which enables the use of young meristematic cells of plumule tip for efficient regeneration and transformation of okra plants. Further, transformed okra plants, plant cells and tissues for improved agronomic/non agronomic traits and insect resistance are produced either by using marker based or marker free systems.
PubMed | Maharashtra Hybrid Seeds Co.
Type: Journal Article | Journal: Plant cell reports | Year: 2013
Agrobacterium -mediated transformation system for okra using embryos was devised and the transgenic Bt plants showed resistance to the target pest, okra shoot, and fruit borer ( Earias vittella ). Okra is an important vegetable crop and progress in genetic improvement via genetic transformation has been impeded by its recalcitrant nature. In this paper, we describe a procedure using embryo explants for Agrobacterium-mediated transformation and tissue culture-based plant regeneration for efficient genetic transformation of okra. Twenty-one transgenic okra lines expressing the Bacillus thuringiensis gene cry1Ac were generated from five transformation experiments. Molecular analysis (PCR and Southern) confirmed the presence of the transgene and double-antibody sandwich ELISA analysis revealed Cry1Ac protein expression in the transgenic plants. All 21 transgenic plants were phenotypically normal and fertile. T1 generation plants from these lines were used in segregation analysis of the transgene. Ten transgenic lines were selected randomly for Southern hybridization and the results confirmed the presence of transgene integration into the genome. Normal Mendelian inheritance (3:1) of cry1Ac gene was observed in 12 lines out of the 21 T0 lines. We selected 11 transgenic lines segregating in a 3:1 ratio for the presence of one transgene for insect bioassays using larvae of fruit and shoot borer (Earias vittella). Fruit from seven transgenic lines caused 100% larval mortality. We demonstrate an efficient transformation system for okra which will accelerate the development of transgenic okra with novel agronomically useful traits.
MAHARASHTRA Hybrid Seeds Company Ltd | Date: 2014-01-15
MAHARASHTRA Hybrid Seeds Company Ltd | Date: 2014-01-15
Seeds, namely, plant seeds and agricultural seeds.