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Baranwal V.K.,University of Delhi | Mikkilineni V.,Maharashtra Hybrid Seeds Company Ltd | Zehr U.B.,Maharashtra Hybrid Seeds Company Ltd | Tyagi A.K.,National Institute of Plant Genome Research | Kapoor S.,University of Delhi
Journal of Experimental Botany | Year: 2012

Perceived by Charles Darwin in many vegetable plants and rediscovered by George H Shull and Edward M East in maize, heterosis or hybrid vigour is one of the most widely utilized phenomena, not only in agriculture but also in animal breeding. Although, numerous studies have been carried out to understand its genetic and/or molecular basis in the past 100 years, our knowledge of the underlying molecular processes that results in hybrid vigour can best be defined as superficial. Even after century long deliberations, there is no consensus on the relative/individual contribution of the genetic/epigenetic factors in the manifestation of heterosis. However, with the recent advancements in functional genomics, transcriptomics, proteomics, and metabolomics-related technologies, the riddle of heterosis is being reinvestigated by adopting systems-level approaches to understand the underlying molecular mechanisms. A number of intriguing hypotheses are converging towards the idea of a cumulative positive effect of the differential expression of a variety of genes, on one or several yield-affecting metabolic pathways or overall energy-use efficiency, as the underlying mechanism for the manifestation of heterosis. Presented here is a brief account of clues gathered from various investigative approaches targeted towards better scientific understanding of this process. © 2012 The Author [2012]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please email: journals.permissions@oup.com.


Bhattacharya A.,Maharashtra Hybrid Seeds Company Ltd | Mikkilineni V.,Maharashtra Hybrid Seeds Company Ltd | Verma L.,Maharashtra Hybrid Seeds Company Ltd | Palan B.,Maharashtra Hybrid Seeds Company Ltd | And 2 more authors.
Indian Journal of Genetics and Plant Breeding | Year: 2014

A study was undertaken to develop an effective protocol to generate doubled haploid plants from a proprietary Mahyco hybrid MRP5401. A total of 20,300 anthers were plated on four different DH media (basal N6 + phytohormones) and obtained a callus induction success rate of 1.52%, of which 0.48% developed into green plants. A total of 232 lines were obtained of which, 98 lines showed homozygosity in DH2-3 generations. Well established DH lines from MRP5401 in DH3 generation had early flowering and yield gain. © 2014, Indian Society of Genetics and Plant Breeding. All rights reserved.


Narendran M.,Maharashtra Hybrid Seeds Co. | Deole S.G.,Maharashtra Hybrid Seeds Co. | Harkude S.,Maharashtra Hybrid Seeds Co. | Shirale D.,Maharashtra Hybrid Seeds Co. | And 5 more authors.
Plant Cell Reports | Year: 2013

Key message: Agrobacterium -mediated transformation system for okra using embryos was devised and the transgenic Bt plants showed resistance to the target pest, okra shoot, and fruit borer (Earias vittella). Okra is an important vegetable crop and progress in genetic improvement via genetic transformation has been impeded by its recalcitrant nature. In this paper, we describe a procedure using embryo explants for Agrobacterium-mediated transformation and tissue culture-based plant regeneration for efficient genetic transformation of okra. Twenty-one transgenic okra lines expressing the Bacillus thuringiensis gene cry1Ac were generated from five transformation experiments. Molecular analysis (PCR and Southern) confirmed the presence of the transgene and double-antibody sandwich ELISA analysis revealed Cry1Ac protein expression in the transgenic plants. All 21 transgenic plants were phenotypically normal and fertile. T1 generation plants from these lines were used in segregation analysis of the transgene. Ten transgenic lines were selected randomly for Southern hybridization and the results confirmed the presence of transgene integration into the genome. Normal Mendelian inheritance (3:1) of cry1Ac gene was observed in 12 lines out of the 21 T0 lines. We selected 11 transgenic lines segregating in a 3:1 ratio for the presence of one transgene for insect bioassays using larvae of fruit and shoot borer (Earias vittella). Fruit from seven transgenic lines caused 100 % larval mortality. We demonstrate an efficient transformation system for okra which will accelerate the development of transgenic okra with novel agronomically useful traits. © 2013 Springer-Verlag Berlin Heidelberg.


The present description concerns methods for regeneration of whole plant from the explants obtained from the Abelmoschus species preferably A. esculentus. In addition the present description also concerns methods for transforming okra plant, plant cells and tissues either with the use of recombinant Agrobacterium strain or by bombarding the explants with tungsten or gold particles coated with DNA sequences of interest. An efficient method to isolate embryos from imbibed seeds of okra is also described which enables the use of young meristematic cells of plumule tip for efficient regeneration and transformation of okra plants. Further, transformed okra plants, plant cells and tissues for improved agronomic/non agronomic traits and insect resistance are produced either by using marker based or marker free systems.


PubMed | Maharashtra Hybrid Seeds Co.
Type: Journal Article | Journal: Plant cell reports | Year: 2013

Agrobacterium -mediated transformation system for okra using embryos was devised and the transgenic Bt plants showed resistance to the target pest, okra shoot, and fruit borer ( Earias vittella ). Okra is an important vegetable crop and progress in genetic improvement via genetic transformation has been impeded by its recalcitrant nature. In this paper, we describe a procedure using embryo explants for Agrobacterium-mediated transformation and tissue culture-based plant regeneration for efficient genetic transformation of okra. Twenty-one transgenic okra lines expressing the Bacillus thuringiensis gene cry1Ac were generated from five transformation experiments. Molecular analysis (PCR and Southern) confirmed the presence of the transgene and double-antibody sandwich ELISA analysis revealed Cry1Ac protein expression in the transgenic plants. All 21 transgenic plants were phenotypically normal and fertile. T1 generation plants from these lines were used in segregation analysis of the transgene. Ten transgenic lines were selected randomly for Southern hybridization and the results confirmed the presence of transgene integration into the genome. Normal Mendelian inheritance (3:1) of cry1Ac gene was observed in 12 lines out of the 21 T0 lines. We selected 11 transgenic lines segregating in a 3:1 ratio for the presence of one transgene for insect bioassays using larvae of fruit and shoot borer (Earias vittella). Fruit from seven transgenic lines caused 100% larval mortality. We demonstrate an efficient transformation system for okra which will accelerate the development of transgenic okra with novel agronomically useful traits.

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