Panchbhai A.,Maharashtra Hybrid Seeds Co. |
Panchbhai A.,Dr. Babasaheb Ambedkar Marathwada University |
Char B.,Maharashtra Hybrid Seeds Co. |
Kharat A.S.,Dr. Babasaheb Ambedkar Marathwada University
Plant Gene | Year: 2017
The most popular CaMV 35S promoter used for the construction of transgenic plants is a constitutive promoter. The use of conditionally expressed promoters would be a better option as the energy towards expression would be accounted for when the need for expression is indicated. The Acacia nilotica has been known to sustain severe moisture stress. The ALDH7 gene has been reported to be an antiquitin. The PCR amplicon for the ALDH7 promoter from A. nilotica was obtained and sequenced from both the ends to decipher nucleotide sequence. Nucleotide sequence analysis of the A. nilotica ALDH7 promoter indicated the presence of a response element. The nucleotide analysis using PLACE indicated presence of the ACGT, drought and salinity stress response element whereas NNPP indicated existence of seven transcriptional start sites and TSSP indicated presence of two promoters. The PCR amplified ALDH7 promoter of A. nilotica was cloned into a binary vector carrying a promoter-less GUS cassette. Thus, the GUS gene could be expressed under the influence of the A. nilotica ALDH7 promoter. Transgenic tobacco plants expressing the GUS gene under the A. nilotica ALDH7 promoter were constructed. Using histochemical staining, the GUS gene expression levels in transgenic tobacco plants during moisture stress and under irrigation were studied. Histochemical staining experiments demonstrated that the GUS gene in transgenic plants was expressed only during moisture stress and remained unexpressed while irrigation was maintained. In contrast, transgenic tobacco plants expressing the GUS gene under the CaMV 35S promoter were found to be constitutive. The histochemical studies also indicated that the GUS expression was stable at least for two generations. Studies of the gene indicated that transgene segregation was in agreement with that of a monogenic trait described by Mendelian inheritance. The ALDH7 promoter of A. nilotica is a conditionally expressible promoter, expressed during moisture stress and suppressed during irrigation. © 2017
Baranwal V.K.,University of Delhi |
Mikkilineni V.,Maharashtra Hybrid Seeds Company Ltd |
Zehr U.B.,Maharashtra Hybrid Seeds Company Ltd |
Tyagi A.K.,National Institute of Plant Genome Research |
Kapoor S.,University of Delhi
Journal of Experimental Botany | Year: 2012
Perceived by Charles Darwin in many vegetable plants and rediscovered by George H Shull and Edward M East in maize, heterosis or hybrid vigour is one of the most widely utilized phenomena, not only in agriculture but also in animal breeding. Although, numerous studies have been carried out to understand its genetic and/or molecular basis in the past 100 years, our knowledge of the underlying molecular processes that results in hybrid vigour can best be defined as superficial. Even after century long deliberations, there is no consensus on the relative/individual contribution of the genetic/epigenetic factors in the manifestation of heterosis. However, with the recent advancements in functional genomics, transcriptomics, proteomics, and metabolomics-related technologies, the riddle of heterosis is being reinvestigated by adopting systems-level approaches to understand the underlying molecular mechanisms. A number of intriguing hypotheses are converging towards the idea of a cumulative positive effect of the differential expression of a variety of genes, on one or several yield-affecting metabolic pathways or overall energy-use efficiency, as the underlying mechanism for the manifestation of heterosis. Presented here is a brief account of clues gathered from various investigative approaches targeted towards better scientific understanding of this process. © 2012 The Author . Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please email: firstname.lastname@example.org.
Bhattacharya A.,Maharashtra Hybrid Seeds Company Ltd |
Mikkilineni V.,Maharashtra Hybrid Seeds Company Ltd |
Verma L.,Maharashtra Hybrid Seeds Company Ltd |
Palan B.,Maharashtra Hybrid Seeds Company Ltd |
And 2 more authors.
Indian Journal of Genetics and Plant Breeding | Year: 2014
A study was undertaken to develop an effective protocol to generate doubled haploid plants from a proprietary Mahyco hybrid MRP5401. A total of 20,300 anthers were plated on four different DH media (basal N6 + phytohormones) and obtained a callus induction success rate of 1.52%, of which 0.48% developed into green plants. A total of 232 lines were obtained of which, 98 lines showed homozygosity in DH2-3 generations. Well established DH lines from MRP5401 in DH3 generation had early flowering and yield gain. © 2014, Indian Society of Genetics and Plant Breeding. All rights reserved.
Narendran M.,Maharashtra Hybrid Seeds Co. |
Deole S.G.,Maharashtra Hybrid Seeds Co. |
Harkude S.,Maharashtra Hybrid Seeds Co. |
Shirale D.,Maharashtra Hybrid Seeds Co. |
And 5 more authors.
Plant Cell Reports | Year: 2013
Key message: Agrobacterium -mediated transformation system for okra using embryos was devised and the transgenic Bt plants showed resistance to the target pest, okra shoot, and fruit borer (Earias vittella). Okra is an important vegetable crop and progress in genetic improvement via genetic transformation has been impeded by its recalcitrant nature. In this paper, we describe a procedure using embryo explants for Agrobacterium-mediated transformation and tissue culture-based plant regeneration for efficient genetic transformation of okra. Twenty-one transgenic okra lines expressing the Bacillus thuringiensis gene cry1Ac were generated from five transformation experiments. Molecular analysis (PCR and Southern) confirmed the presence of the transgene and double-antibody sandwich ELISA analysis revealed Cry1Ac protein expression in the transgenic plants. All 21 transgenic plants were phenotypically normal and fertile. T1 generation plants from these lines were used in segregation analysis of the transgene. Ten transgenic lines were selected randomly for Southern hybridization and the results confirmed the presence of transgene integration into the genome. Normal Mendelian inheritance (3:1) of cry1Ac gene was observed in 12 lines out of the 21 T0 lines. We selected 11 transgenic lines segregating in a 3:1 ratio for the presence of one transgene for insect bioassays using larvae of fruit and shoot borer (Earias vittella). Fruit from seven transgenic lines caused 100 % larval mortality. We demonstrate an efficient transformation system for okra which will accelerate the development of transgenic okra with novel agronomically useful traits. © 2013 Springer-Verlag Berlin Heidelberg.