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Goyal A.K.,North Bengal University | Middha S.K.,Maharani Lakshmi Ammanni College for Women | Sen A.,North Bengal University
Indian Journal of Natural Products and Resources | Year: 2013

vulgaris Schrad. ex J. C. Wendl. var. vittata Riviere & C. Riviere (BVV) leaves are consumed as food and have antimicrobial activity. Positive health effect of its consumption is attributed to the bioactive substances. In the present study, the influence of BVV antioxidants on oxidative stress that can be used as an alternative option has been studied. The total phenol and flavonoids content in the aqueous and acetone extract were determined by calorimetric assay, as well as their antioxidant activity through various chemical assays like, DPPH radical, Hydrogen peroxide scavenging and reducing power assay. Both the aqueous and acetone extracts displayed their antioxidantactivity in a dose dependent manner by scavenging hydrogen peroxide and DPPH radical. The total phenolic and total flavonoids compounds in both aqueous and acetone extract were 345.910.071 and 325.630.051 mg GAE/g of dry extract and 289.20.05 and 179.20.002 mg quercetin/g of dry extract, respectively. This may be the first report to provide evidence that the crude aqueous and acetone extract of Bambusa vulgaris var. vittata leaf is a potential source of natural antioxidants. Source


Kumari M.R.,Maharani Lakshmi Ammanni College for Women
Rheedea | Year: 2013

Solanum diphyllum L., a tropical American species, is added here to the flora of Southern India from Bangalore, Karnataka. Its occurrence in Maharashtra and Tamil Nadu is discussed. A detailed description and photograph of the species are provided for easy identification. Source


Middha S.K.,Kumaun University | Usha T.,Maharani Lakshmi Ammanni College for Women | Pande V.,Kumaun University
Advances in Pharmacological Sciences | Year: 2013

This study revealed polyphenolic content, nutritive content, antioxidant activity, and phenolic profile of methanol and aqueous extracts of Punica granatum peel extract. For this, extracts were screened for possible antioxidant activities by free radical scavenging activity (DPPH), hydrogen peroxide scavenging activity and ferric-reducing antioxidant power (FRAP) assays. The total phenolics and flavonoid recovered by methanolic (MPE) and the water extract (AQPE) were ranged from 185 ± 12.45 to 298.00 ± 24.86 mg GAE (gallic acid equivalents)/gm and 23.05 ± 1.54 to 49.8 ± 2.14 quercetin (QE) mg/g, respectively. The EC50 of herbal extracts ranged from 100 μg/ml (0.38 quercetin equivalents), for AQPE, 168 μg/ml (0.80 quercetin equivalents), for MPE. The phenolic profile in the methanolic extracts was investigated by chromatographic (HPLC) method. About 5 different flavonoids, phenolic acids, and their derivatives including quercetin (1), rutin (2), gallic acid (3), ellagic acid (4), and punicalagin as a major ellagitannin (5) have been identified. Among both extracts, methanolic extract was the most effective. This report may be the first to show nutritive content and correlation analysis to suggest that phenols and flavonoids might contribute the high antioxidant activity of this fruit peel and establish it as a valuable natural antioxidant source applicable in the health food industry. © 2013 Sushil Kumar Middha et al. Source


Mishra T.,North Bengal University | Goyal A.K.,North Bengal University | Middha S.K.,Maharani Lakshmi Ammanni College for Women | Sen A.,North Bengal University
Indian Journal of Natural Products and Resources | Year: 2011

Canna edulis Ker- Gawl., found in the sub-Himalayan region contains starchy rhizome having economic importance in terms of food and herbal medicine. Correlation of phytochemical characteristics and antioxidative properties have been studied in hot and cold extracts of C. edulis rhizome. Antioxidants, BHT, quercetin, gallic acid and ascorbic acid equivalents have been determined using spectrophotometer. Hydrogen peroxide scavenging ability and reducing power properties have been used to define in vitro radical scavenging activity of both extracts. Total phenol and flavonoid contents were highest in hot extract (42.71 mg GAE/g and 21.92 mg QE/g) than cold extract (33.7 mg GAE/g and 15.12 mg QE/g). IC 50 value of DPPH and H 2O 2 were also observed to be higher in hot extract. The electron donation ability (EDA) of the hot extract (500 μg/ml) demonstrated stronger activity than cold extract (400 μg/ml). Further evaluation of free radicals was done by determining the specific Rf value of bioactive components through TLC. Thus the hot rhizome extract of C. edulis exerts more effective antioxidant property as compared to cold extract. Source


Middha S.K.,Kumaun University | Usha T.,Maharani Lakshmi Ammanni College for Women | Pande V.,Kumaun University
Evidence-based Complementary and Alternative Medicine | Year: 2013

Over the past decade, pomegranate (Punica granatum) is entitled as a wonder fruit because of its voluminous pharmacological properties. In 1830, P. granatum fruit was first recognized in United States Pharmacopeia; the Philadelphia edition introduced the rind of the fruit, the New York edition the bark of the root and further 1890 edition the stem bark was introduced. There are significant efforts and progress made in establishing the pharmacological mechanisms of peel (pericarp or rind) and the individual constituents responsible for them. This review provides an insight on the phytochemical components that contribute too antihyperglycemic, hepatoprotective, antihyperlipidemic effect, and numerous other effects of wonderful, economic, and eco-friendly pomegranate peel extract (PP). © 2013 Sushil Kumar Middha et al. Source

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