Maharani Lakshmi Ammanni College for Women

Bangalore, India

Maharani Lakshmi Ammanni College for Women

Bangalore, India

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Middha S.K.,Maharani Lakshmi Ammanni College for Women | Bhattacharjee B.,Near Corporation Circle | Saini D.,Maharani Lakshmi Ammanni College for Women | Baliga M.S.,Father Muller Medical College | And 2 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2011

Background and Objectives: "In all things of nature there is something of the marvelous." In the present study the anti-hyperglycemic and anti-oxidative potential of aqueous extract of Trigonella foenum graceum (TFG), a traditional medicinal herb was assessed in liver and WBC of alloxan induced diabetic rats. Free radicals can cause oxidative damage, which is balanced by the antioxidants. This has been implicated in aging, and diseases such as diabetes and other chronic conditions. Material and Methods: TFG extract was administered orally [500 (LM) and 1000 mg/kg body weight (HM)] for six weeks. The effect of TFG on blood glucose were studied and the levels of lipid peroxidation [MDA (Malondialdehyde)] and antioxidant enzymes [SOD (Superoxide dismutase), GPx (Reduced Glutathione peroxidase)] were estimated and compared with standard drugs glibenclamide and insulin. Results: Treatment with TFG, insulin and glibenclamide resulted in significantly reduced blood glucose in LM (8.71%) and HM (3.87%) in comparison with normal controls. There was a significant decrease in lipid peroxidation in liver and white blood cells (WBC) in both low and high doses [liver LM (49%), HM (57.25%)], [WBC LM (54.28%), HM (62.5%)] and increase in antioxidant enzymes SOD [liver LM (33.59%), HM (58.7%)] [WBC LM (44.9%)] HM (58.7%) and GPx [Liver LM (58.55%), HM (40.20%)], [WBC LM (55.46%), HM (56.4%)] when compared to diabetic controls. Discussion: Potency of TFG in restoring several parameters to normal values is comparable to glibenclamide, though not as efficient as insulin, an indication of its antihyperglycemic and antioxidant effect.

Middha S.K.,Maharani Lakshmi Ammanni College for Women | Goyal A.K.,North Bengal University | Faizan S.A.,M.S. Ramaiah Institute of Technology | Sanghamitra N.,M.S. Ramaiah Institute of Technology | And 2 more authors.
Journal of Biosciences | Year: 2013

Type 2 diabetes is an inevitably progressive disease, with irreversible β cell failure. Glycogen synthase kinase and Glukokinase, two important enzymes with diverse biological actions in carbohydrate metabolism, are promising targets for developing novel antidiabetic drugs. A combinatorial structure-based molecular docking and pharmacophore modelling study was performed with the compounds of Hippophae salicifolia and H. rhamnoides as inhibitors. Docking with Discovery Studio 3.5 revealed that two compounds from H. Salicifolia, viz Lutein D and an analogue of Zeaxanthin, and two compounds from H. rhamnoides, viz Isorhamnetin-3-rhamnoside and Isorhamnetin-7-glucoside, bind significantly to the GSK-3 β receptor and play a role in its inhibition; whereas in the case of Glucokinase, only one compound from both the plants, i.e. vitamin C, had good binding characteristics capable of activation. The results help to understand the type of interactions that occur between the ligands and the receptors. Toxicity predictions revealed that none of the compounds had hepatotoxic effects and had good absorption as well as solubility characteristics. The compounds did not possess plasma protein-binding, crossing blood-brain barrier ability. Further, in vivo and in vitro studies need to be performed to prove that these compounds can be used effectively as antidiabetic drugs. © 2013 Indian Academy of Sciences.

Mishra T.,North Bengal University | Goyal A.K.,North Bengal University | Middha S.K.,Maharani Lakshmi Ammanni College for Women | Sen A.,North Bengal University
Indian Journal of Natural Products and Resources | Year: 2011

Canna edulis Ker- Gawl., found in the sub-Himalayan region contains starchy rhizome having economic importance in terms of food and herbal medicine. Correlation of phytochemical characteristics and antioxidative properties have been studied in hot and cold extracts of C. edulis rhizome. Antioxidants, BHT, quercetin, gallic acid and ascorbic acid equivalents have been determined using spectrophotometer. Hydrogen peroxide scavenging ability and reducing power properties have been used to define in vitro radical scavenging activity of both extracts. Total phenol and flavonoid contents were highest in hot extract (42.71 mg GAE/g and 21.92 mg QE/g) than cold extract (33.7 mg GAE/g and 15.12 mg QE/g). IC 50 value of DPPH and H 2O 2 were also observed to be higher in hot extract. The electron donation ability (EDA) of the hot extract (500 μg/ml) demonstrated stronger activity than cold extract (400 μg/ml). Further evaluation of free radicals was done by determining the specific Rf value of bioactive components through TLC. Thus the hot rhizome extract of C. edulis exerts more effective antioxidant property as compared to cold extract.

PubMed | Padmashree Institute of Management and Sciences, Bodoland University, Maharani Lakshmi Ammanni College for Women and Kalimpong College
Type: Journal Article | Journal: Antioxidants (Basel, Switzerland) | Year: 2016

Despite several pharmacological applications of Baccaurea ramiflora Lour., studies on the influence of its polyphenol content on pharmacological activity such as anti-inflammatory properties have been scarce. Here we evaluated in vitro antioxidant activity, poyphenolics by HPLC and the anti-inflammatory potential of the methanolic leaf extract of Baccaurea ramiflora (BME) and its protective effects in carrageenan-induced paw edema model of inflammation in rats. The BME extract contained 79.06 0.03 mg gallic acid equivalent (GAE)/g total polyphenols, 28.80 0.01 mg quercetin equivalent (QE)/g flavonoid and 29.42 0.01 g cathechin equivalent/g proanthocyanidin respectively and rosmarinic acid (8 mg/kg) as a main component was identified by HPLC. Results demonstrate that administration of BME at the dose of 200 mg/kg can reduce paw edema by over 63%, and it exhibits a dose-response effect. Depending on concentration, the extract exerted scavenging activity on DPPH radical (IC50 36.4 g/mL), significantly inhibited IL-1 (4.4 pg/mg protein) and TNF- (0.21 ng/g protein). Therefore, we conclude BME causes a substantial reduction of inflammation in in vivo models. We propose that rosmarinic acid and similar phenolic compounds may be useful in the therapy of inflammation-related injuries.

Middha S.K.,Kumaun University | Usha T.,Maharani Lakshmi Ammanni College for Women | Pande V.,Kumaun University
Evidence-based Complementary and Alternative Medicine | Year: 2013

Over the past decade, pomegranate (Punica granatum) is entitled as a wonder fruit because of its voluminous pharmacological properties. In 1830, P. granatum fruit was first recognized in United States Pharmacopeia; the Philadelphia edition introduced the rind of the fruit, the New York edition the bark of the root and further 1890 edition the stem bark was introduced. There are significant efforts and progress made in establishing the pharmacological mechanisms of peel (pericarp or rind) and the individual constituents responsible for them. This review provides an insight on the phytochemical components that contribute too antihyperglycemic, hepatoprotective, antihyperlipidemic effect, and numerous other effects of wonderful, economic, and eco-friendly pomegranate peel extract (PP). © 2013 Sushil Kumar Middha et al.

Middha S.K.,Kumaun University | Usha T.,Maharani Lakshmi Ammanni College for Women | Pande V.,Kumaun University
Advances in Pharmacological Sciences | Year: 2013

This study revealed polyphenolic content, nutritive content, antioxidant activity, and phenolic profile of methanol and aqueous extracts of Punica granatum peel extract. For this, extracts were screened for possible antioxidant activities by free radical scavenging activity (DPPH), hydrogen peroxide scavenging activity and ferric-reducing antioxidant power (FRAP) assays. The total phenolics and flavonoid recovered by methanolic (MPE) and the water extract (AQPE) were ranged from 185 ± 12.45 to 298.00 ± 24.86 mg GAE (gallic acid equivalents)/gm and 23.05 ± 1.54 to 49.8 ± 2.14 quercetin (QE) mg/g, respectively. The EC50 of herbal extracts ranged from 100 μg/ml (0.38 quercetin equivalents), for AQPE, 168 μg/ml (0.80 quercetin equivalents), for MPE. The phenolic profile in the methanolic extracts was investigated by chromatographic (HPLC) method. About 5 different flavonoids, phenolic acids, and their derivatives including quercetin (1), rutin (2), gallic acid (3), ellagic acid (4), and punicalagin as a major ellagitannin (5) have been identified. Among both extracts, methanolic extract was the most effective. This report may be the first to show nutritive content and correlation analysis to suggest that phenols and flavonoids might contribute the high antioxidant activity of this fruit peel and establish it as a valuable natural antioxidant source applicable in the health food industry. © 2013 Sushil Kumar Middha et al.

Middha S.K.,Maharani Lakshmi Ammanni College for Women | Usha T.,Maharani Lakshmi Ammanni College for Women | RaviKiran T.,Bangalore University
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: The present study was carried out to investigate the effects of Punica granatum peel methanolic extract (PGPE) on cerebral cortex (CC) and Hippocampus (HC) brain antioxidant defense system and markers of lipid and protein oxidation in alloxan induced diabetic rats. Methods: Oral administration of PGPE (75 and 150 mg of kg body weight) for 45 days resulted in significant reduction in blood glucose levels. Results: Supplementation of diabetic rats with PGPE showed increased activities of SOD and GPx with concomitant decrease in MDA and PC content. Region-specific changes were more evident in the HC when compared to CC. Conclusions: The present study indicated that PGPE can ameliorate brain oxidative stress in alloxan induced diabetic rats by up regulating antioxidant defense mechanism by attenuating lipid and protein oxidation. PGPE thus may be used as a potential therapeutic agent in preventing diabetic complications in the brain. © 2012 Asian Pacific Tropical Biomedical Magazine.

Ravi Kiran T.,Bangalore University | Aruna H.K.,Maharani Lakshmi Ammanni College for Women
Italian Journal of Zoology | Year: 2010

The main aim of this work is to provide basic data on the aspects of pro-oxidant and antioxidant processes in different life stages of Eudrilus eugeniae. The levels of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), along with the byproducts of lipid and protein oxidation, malondialdehyde (MDA) and protein carbonyl (PC), content were determined in the whole body of juvenile, sub-adult and adult earthworms. The SOD activity significantly (P > 0.05) decreased during development from juvenile to adult, while CAT activity showed an increase with advancing age. The MDA content significantly increased in the adult compared to other age groups. The protein carbonyl content showed insignificant changes with respect to age. Our results suggest that the decline in SOD activity coupled with increased levels of MDA implicate the susceptibility of older animals to oxidative stress. © 2010 Unione Zoologica Italiana.

Shashikala A.,Maharani Lakshmi Ammanni College for Women
International Journal of Pharma and Bio Sciences | Year: 2016

Propolis is a resinous Bee hive product and has been long used in folk medicine of different nations as early as 3000 BC. Stingless bees are indigenous pollinators of treasured herbal plants. Propolis of stingless bees is a natural, strongly adhesive mixture made of bee secretions and plant resins. Stingless bee Propolis exhibit a wide spectrum of pharmacological activities that vary depending on the geographical location, climate, generic type of bees and availability of botanical sources for the bees to forage. The present study was carried out to identify the phytocomponents present in the methanolic extract of the propolis of stingless bees reared and collected from Devanahalli, Bangalore, by GC-MS analysis. From the GC-MS results five compounds were identified as major constituents, they are Ethylhexanol, 1,1 dimethylethyl, 4,6 dimethyl, Diethyl phthalate, 1,2 benezenedicarboxylic acid. These different active phytochemicals have been found to possess a wide range of Pharmacological activities, which may help in the protection against incurable diseases.

Kumari M.R.,Maharani Lakshmi Ammanni College for Women
Rheedea | Year: 2013

Solanum diphyllum L., a tropical American species, is added here to the flora of Southern India from Bangalore, Karnataka. Its occurrence in Maharashtra and Tamil Nadu is discussed. A detailed description and photograph of the species are provided for easy identification.

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