Yang C.-C.,National Taiwan Normal University |
Yang S.-Y.,National Taiwan Normal University |
Yang S.-Y.,MagQu Co. |
Chieh J.-J.,National Taiwan Normal University |
And 8 more authors.
ACS Chemical Neuroscience
Magnetic nanoparticles biofunctionalized with antibodies against β-amyloid-40 (Aβ-40) and Aβ-42, which are promising biomarkers related to Alzheimer's disease (AD), were synthesized. We characterized the size distribution, saturated magnetizations, and stability of the magnetic nanoparticles conjugated with anti-Aβ antibody. In combination with immunomagnetic reduction technology, it is demonstrated such biofunctionalized magnetic nanoparticles are able to label Aβs specifically. The ultralow-detection limits of assaying Aβs in vitro using the magnetic nanoparticles via immunomagnetic reduction are determined to a concentration of ~10 ppt (10 pg/mL). Further, immunomagnetic reduction signals of Aβ-40 and Aβ-42 in human plasma from normal samples and AD patients were analyzed, and the results showed a significant difference between these two groups. These results show the feasibility of using magnetic nanoparticles with Aβs as reagents for assaying low-concentration Aβs through immunomagnetic reduction, and also provide a promising new method for early diagnosis of Alzheimer's disease from human blood plasma. © 2011 American Chemical Society. Source
Chiu M.-J.,National Taiwan University |
Chen Y.-F.,National Taiwan University Hospital |
Chen T.-F.,National Taiwan University |
Yang S.-Y.,National Taiwan Normal University |
And 9 more authors.
Human Brain Mapping
Neurofibrillary tangles are associated with cognitive dysfunction, and hippocampal atrophy with increased CSF tau markers. However, the plasma tau levels of Alzheimer's disease (AD) have not been well studied. We investigated plasma tau by using an immunomagnetic reduction assay in 20 patients with mild cognitive impairment (MCI) due to AD, 10 early AD dementia, and 30 healthy elders (HE). All received a 3D-brain MRI scan and a set of cognitive function test. We explored their relationships with both brain structure and cognitive functions. Images were analyzed to determine the brain volumes and gray matter densities. Patients with MCI or early AD had significantly increased plasma tau levels compared with HE. Plasma tau levels were negatively associated with the performance of logical memory, visual reproduction, and verbal fluency; also negatively associated with volume of total gray matter, hippocampus, amygdala; and gray matter densities of various regions. Regression analyses indicated that logical memory explained 0.394 and hippocampus volume predicted. 608 of the variance of plasma tau levels, both P < 0.001. Education years were negatively associated with the gray matter densities of the supramarginal (r = -0.407), middle temporal gyrus (r = -0.40) and precuneus (r = -0.377; all P < 0.05) in HE; and negatively associated with plasma tau levels in patients (r = -0.626). We propose that plasma tau may serve as a window to both structure and function of the brain. Higher education is a protective factor against AD and is associated with lower plasma tau levels in patients. © 2013 Wiley Periodicals, Inc. Source
MAGQU Co. | Date: 2011-08-09
The invention provides a low-field nuclear magnetic resonance system for measuring a magnetic resonance signal of an object. The low-field nuclear magnetic resonance system includes a pre-magnetization module, a uniform magnetic field coil module, a pulse and receiving coil module, a filter amplifier module, a signal acquisition module and a processing module. The pre-magnetization module is used to establish a pre-magnetization field in the object to increase magnetization of the object. The uniform magnetic field coil module is used to change a resonance frequency and background magnetic field intensity of the object during nuclear magnetic resonance measurement by regulating a magnetic field of the coil. The processing module further includes a programming object for controlling timing processing and signal analysis of the measurement process.
MAGQU Co. | Date: 2010-10-22
Present invention relates to a device magnetically separating a sample. The preferred material for the device is plastic. The device is a cuboid plate, which consists of one or two bottom sides and at least two lateral sides. The bottom side has wells on it to accommodate magnetic elements, and the magnetic element which is suitable to be accommodated into the wells comprising column shape, half-column shape, or half circular column shape magnetic elements. The magnetic element comprises permanent magnet or electromagnet, and the permanent magnet is selected from the group consisting of alnico, samarium cobalt, neodymium iron boron, or magnetic ceramic materials. The magnetic elements can adsorb the magnetic samples in the micro plate.
MAGQU Co. | Date: 2014-03-07
The present invention provides a method for detecting the risk of Alzheimers disease, comprising detecting immunomagnetic reduction signals of two biological markers in a biological sample from a subject, wherein the two biological markers is tau protein and A-42 protein, respectively; and calculating concentrations of the above two biological markers and using the product of the concentrations of the above two biological markers to diagnosing the risk of Alzheimers disease, wherein the concentration is calculated by the conversion of the magnetic reduction signals.