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Sugimoto M.,National Livestock Breeding Center | Itoh T.,Maebashi Institute of Animal Science | Gotoh Y.,Holstein Cattle Association of Japan | Kawahara T.,Holstein Cattle Association of Japan | And 3 more authors.
Journal of Dairy Science | Year: 2011

Mastitis is a common infectious disease of the mammary gland and a major problem in the dairy industry. We previously reported that forebrain embryonic zinc finger-like (FEZL) encoding a stretch of 12 glycines (p.Gly105[12]) instead of 13 glycines (p.Gly105[13]) is associated with a lower somatic cell score (SCS) in a family derived from Walkway Chief Mark. Here we report that the p.Gly105[12] allele is associated with a significantly decreased incidence of clinical mastitis in a large Holstein population. We genotyped the FEZL polymorphism in 918 randomly collected Holstein sires, and investigated the effect of the polymorphism on the estimated breeding value (EBV) for SCS and milk, fat, solids-not-fat, and protein yield, and on the number of cattle with clinical mastitis among daughters derived from these sires. The average EBV for SCS among sires carrying the heterozygous p.Gly105[12] was significantly lower than that among sires carrying the homozygous p.Gly105[13], whereas we found no unfavorable effects of this polymorphism on EBV for milk, fat, solids-not-fat, and protein yield. The proportion of cows with clinical mastitis derived from sires carrying heterozygous p.Gly105[12] was significantly lower than that of daughters derived from sires carrying the homozygous p.Gly105[13]. Thus, selection of sires carrying p.Gly105[12] could be beneficial in the dairy industry by reducing the incidence of mastitis. © 2011 American Dairy Science Association.

Komatsu M.,National Institute of Livestock and Grassland Science NILGS | Fujimori Y.,Ibaraki Prefecture Livestock Research Center | Sato Y.,Iwate Prefecture Livestock Research Center | Okamura H.,Japan National Institute of Agrobiological Science | And 12 more authors.
Animal Science Journal | Year: 2010

Growth hormone secretagogue receptor 1a (GHSR1a) mediates the different actions of its endogenous ligand, ghrelin. Ghrelin-GHSR is involved in many important functions that include growth hormone secretion and food intake. We evaluated the haplotype variety and characterized the microsatellite ((TG)n, 5'-UTR) and nucleotide polymorphisms of the bovine GHSR1a gene. The nucleotide sequencing of this gene (∼6 kb) revealed 47 single nucleotide polymorphisms (SNPs), four indels and the microsatellite ((GTTT)n, Intron 1). The 19 haplotypes were constructed from all nucleotide viability patterns and were divided into three major groups. Four SNPs (L24V, nt456(G>A), D191N and nt667(C>T)) and DelR242 in Exon 1 and a haplotype block of approximately 2.2 kb (nt667(C>T) ∼ nt2884 (A>G)) were found in Bos taurus breeds. Breed differences in allele frequencies of the two microsatellites, nt-7(C>A), L24V, and DelR242 loci were found (P < 0.005). A DelR242 was found in the Japanese Shorthorn (frequency: ∼ 0.44), Japanese Brown, five European cattle breeds, the Philippine native cattle, but none detected in the Japanese Black or the Mishima island cattle. Additionally, 5'-rapid amplification of cDNA ends and RT-PCR analyses revealed that there were two different kinds of transcripts: spliced, without a microsatellite within 5'-UTR (GHSR1a); and non-spliced, with the microsatellite (GHSR1b). © 2010 The Authors; Journal compilation © 2010 Japanese Society of Animal Science.

Yamada T.,Niigata University | Sasaki S.,Maebashi Institute of Animal Science | Sukegawa S.,Tsukuba Center Inc. | Takahagi Y.,Tsukuba Center Inc. | And 4 more authors.
Journal of Animal and Veterinary Advances | Year: 2011

Growth performance as well as marbling is the main breeding objectives in Japanese Black cattle, the major beef breed in Japan. The researchers have recently reported that a Single Nucleotide Polymorphism (SNP), referred to asg.231054C>T in promoter region of the Titin (TTN) gene was associated with marbling in Japanese Black beef cattle with the T allele being associated with a high level of marbling. The TTN is known to be involved in myofibrillogenesis and has been previously shown to be located within genomic region of a quantitative trait locus for rib eye area. Thus, the TTN was considered as a positional functional candidate for the gene responsible for growth performance. In this study, the researchers analyzed the effect of the TTN g.231054C>T SNP genotypes on the growth-related carcass traits in Japanese Black beef cattle. The SNP was marginally associated with rib eye area in two experiments using 101 sires (p = 0.067) and 820 paternal half-sib progeny steers from 3 sires homozygous for C allele at the g.231054C>T (p = 0.090) in Japanese Black beef cattle. These findings suggest possible effect of the g.231054C>T on the growth-related trait in Japanese Black beef cattle. The TTN SNP polymorphism may be useful for effective marker-assisted selection to increase the beef productivity in Japanese Black beef cattle. © Medwell Journals, 2011.

Komatsu M.,National Institute of Livestock | Itoh T.,Maebashi Institute of Animal Science | Fujimori Y.,Ibaraki Prefecture Livestock Research Center | Satoh M.,National Institute of Livestock | And 5 more authors.
Animal Science Journal | Year: 2011

We carried out a genetic association study between five nucleotide polymorphisms (5'UTR microsatellite ((TG)n), nt-7(C>A), L24V, DelR242 and Intron 1 microsatellite) of the GHSR1a gene and growth and carcass traits in 1285 steers sired by 117 Japanese Black bulls in a progeny testing program. We report herein, a significant association between the 5'UTR microsatellite and nt-7(C>A) loci and growth and carcass traits. We also propose a translational hypothesis that the association is due to differences in the secondary structure of GHSR1b mRNA (the non-spliced type with the 5'UTR microsatellite) among the GHSR1a gene haplotypes. Furthermore, we predicted the potential increase in profitability due to increased carcass weight in cow-calf fattening enterprises through planned matings based on DNA testing of the 5'UTR microsatellite. Statistical analysis revealed that the 5'UTR microsatellite locus had a significant additive effect on carcass weight (CW) and average daily gain (ADG), but not on beef marbling score (BMS). One of the four major microsatellite alleles (19-TG allele) with an allele frequency of 0.145, had a significantly (P<0.0007) desirable effect on CW and ADG. We concluded that the 19-TG allele could potentially be economically useful nucleotide markers for growth and carcass traits in Japanese Black cattle. © 2011 Japanese Society of Animal Science.

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