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Parga-Lozano C.,Complutense University of Madrid | Rey-Medrano D.,Complutense University of Madrid | Gomez-Prieto P.,Complutense University of Madrid | Areces C.,Complutense University of Madrid | And 4 more authors.
Molecular Biology Reports | Year: 2011

Amerindians immigrated to Madrid (Spain) region are about 10% of the present day population. Amerindians are believed to be the first American inhabitants, before Na-Dene speakers, Aleuts and Eskimo. They may initially have arrived to America from Siberia and also from other parts of Pacific Sea (South Asia, Polynesia and Australia). Nowadays, they populate America from Canada to Tierra del Fuego (South America tip South). Most Amerindian immigrants to Madrid have come from Andean Countries in the last 10 years (mainly Ecuador, Bolivia, Peru and Colombia). They show an HLA profile with "quasi-specific alleles", which makes them different to the rest of the World. In the present work, we have aimed to determine the immigrants HLA profile in order to establish a virtual transplantation waiting list which may be useful for their therapeutic transplants, particularly bone marrow transplantation. This would be carried out together with Amerindian immigrants to other parts of Spain and with their own countries in order to build up transplantation programs. Specific epidemiology programs on HLA linked disease will also be established. Immigrant volunteer unrelated blood donors contributed to the present study. HLA typing was performed by standard methods and their HLA profile obtained and obtained and compared with 15,108 HLA chromosomes from the rest of the World, including Spaniards. The immigrants showed a typical Amerindian profile similar to isolated Amerindian ethnic groups and altogether different to other World inhabitants (including Spaniards). These are the first bases to set up transplantation and epidemiology studies in collaboration with their original population in America. Finally, the HLA profile found in these Amerindians does not indicate their American specific original area, as it is expected from previous studies, i.e.: they do not relate more with Andean than with other Amerindians in Neighbour Joining dendrograms or correspondence analyses. © Springer Science+Business Media B.V. 2010.


Rey D.,Complutense University of Madrid | Areces C.,Complutense University of Madrid | Alonso-Rubio J.,Complutense University of Madrid | Enriquez-de-Salamanca M.,Complutense University of Madrid | And 6 more authors.
Molecular Biology Reports | Year: 2013

HLA-A, -B, -DQB1, and -DRB1 typing has been performed in a sample of Georgian population (South Caucasus). Allele frequencies, neighbour joining and correspondence relatedness analyses and extended HLA haplotypes have been obtained with comparison with other Middle East andMediterranean populations.OurGeorgian sample tends to be genetically related in these analyses with Eastern Mediterraneans and Middle East people. This is important for future regional transplant programs, and Georgian HLA and disease epidemiology and pharmacogenomics.© Springer Science+Business Media Dordrecht 2013.


Arnaiz-Villena A.,Complutense University of Madrid | Areces C.,Complutense University of Madrid | Enriquez-de-Salamanca M.,Complutense University of Madrid | Abd-El-Fatah-Khalil S.,Madrid Regional Blood Center | And 4 more authors.
Immunological Investigations | Year: 2014

HLA-A, -B and -DRB1 alleles have been studied in a Mixtec Mexican Amerindian population by indirect DNA sequencing. HLA relatedness has been tested by comparing results with other Amerindians and worldwide populations; a total of 15,681 chromosomes have been used. Genetic distances between populations, Neighbour Joining (NJ) dendrograms and correspondence analyses have been carried out. Conclusions are: 1) Our Mixtec sample from Oaxaca Coastal Mexican area shows an HLA profile different to that of Oaxaca Central Mountains area showing that genes and languages do not correlate which is inferred both by plane genetic distances and NJ dendrograms and correspondence analyses. 2) Genetic distances and NJ dendrograms join together Mazatecan Mexican Amerindians with our studied Coastal Mixtec group; it fits with the historical relationship between Mixtec and Mazatecans. 3) A∗24:02-B∗35:14-DRB1∗04:11, A∗02:01-B∗15:15-DRB1∗04:11 and A∗68:03-B∗39:08-DRB1∗08:02 extended HLA haplotypes have been "de novo" found in our Mixtec Coastal sample. 4) Shared HLA alleles are found between our Pacific Coast Mixtec Amerindians and Pacific Islanders. 5) These results are useful for establishing a future area transplantation waiting list, for the study of HLA linked diseases epidemiology and for pharmacogenomics in certain drug therapy. © Informa Healthcare USA, Inc.


Rey D.,Complutense University of Madrid | Amirzargar A.,Tehran University of Medical Sciences | Areces C.,Complutense University of Madrid | Enriquez-De-Salamanca M.,Complutense University of Madrid | And 6 more authors.
Immunological Investigations | Year: 2015

HLA class I and II alleles have been studied in a population from Gorgan (North East Iranian city bordering Turkmenistan). This population is composed of mainly Turkmen who speak Oghuz Turkish language. Comparison of Gorgan people HLA profile has been carried out with about 7984 HLA chromosomes from other worldwide populations; extended haplotypes and three dimension genetic distances have been calculated by using neighbor-joining and correspondence relatedness analyses. Most frequent extended HLA haplotypes show a Siberian/Mediterranean admixture and closest populations are Chuvashians (North Caspian Sea, Russia) and other geographically close populations like Siberian Mansi, Buryats and other Iranians. New extended HLA haplotypes have been found, such as: A∗31:01-B∗35:01-DRB1∗15:01-DQB1∗03:01, A∗01:01-B∗35:01-DRB1∗03:01-DQB1∗02:01. Relationships of Turkmen with Kurgan (Gorgan) archaeological mounds, Scythians and Sarmatians are discussed. This study is also useful for a future transplantation Gorgan waiting list, Gorgan HLA and disease epidemiology and HLA pharmacogenomics. © 2015 Informa Healthcare USA, Inc.


Arnaiz-Villena A.,Complutense University of Madrid | Abd-El-Fatah S.,Madrid Regional Blood Center | Granados-Silvestre M.A.,National Autonomous University of Mexico | Parga-Lozano C.,Complutense University of Madrid | And 8 more authors.
Genetic Testing and Molecular Biomarkers | Year: 2011

Background: The major histocompatibility complex genes are located on the short arm of the human sixth chromosome; they are highly polymorphic and therefore have been very advantageous in population genetic studies. Methods: A Mazahua group established in North Mexico State and also in nearby Michoacan state in the rainy mountain highlands (Mexico) was studied for their human leukocyte antigen (HLA)-DRB1 alleles. The relationship with other Amerindians and worldwide populations was studied by using 14, 996 chromosomes from 75 different populations and calculating neighbor-joining dendrograms and correspondence multidimensional values. Results: Five principal HLA allele frequencies were found in our group: DRB1*0802 (the most frequent one in this population), DRB1*0407, DRB1*0403, DRB1*0101, and DRB1*1406. Both genetic distances and correspondence analyses clearly show that our Mazahua group is genetically close to some of the most ancient groups living in Mexico (Mayos, Zapotecans, Tennek) and South American Amerindians. Amerindians remain as a group apart from the rest of the world. Conclusions: The results analyzing the HLA-DR locus suggest that Mazahua language (Otomangue) does not correlate with those of the most closely HLA-correlated ethnic groups. The present data may be useful for future transplantation programs, HLA and disease diagnosis, and pharmacogenetic studies. Copyright 2011, Mary Ann Liebert, Inc.

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