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Gessoni G.,Madonna della Navicella Hospital | Saccani G.,Clinical Chemistry and Microbiology Laboratory | Valverde S.,Madonna della Navicella Hospital | Manoni F.,Civil Hospital | Caputo M.,Clinical Chemistry and Microbiology Laboratory
Clinica Chimica Acta | Year: 2015

Urine culture is the most frequently requested test for a Microbiology Lab. A reliable screening tool would be of paramount importance both to clinicians and laboratorians, provided that it could get fast and accurate negative results in order to rule-out urinary tract infection (UTI). Materials and methods: We evaluated 1907 consecutive urine samples from outpatients. Culture was performed on chromogenic agar with 1μL loop, using 105CFU/mL as a limit of positive growth. Using Sysmex Uf-1000i analyzer we evaluated bacteria forward scatter (B_FSC) and fluorescent light scatter (B_FLH) in a preliminary discrimination step for UTI caused by Gram+ or Gram- bacteria. Results: We got 512 positive samples. A mono-microbial infection was observed in 490 samples; two bacterial strains were isolated in 22 samples, so 534 bacterial strains were found: 392 Gram-, 133 Gram. + and 9 yeasts. Comparing Gram. + and Gram. - bacteria we observed a statistically significant difference for B_FSC but not for B_FLH. In this application experimental cut-off value for B_FSC was 25ch. Using this cut-off to perform a presumptive identification of UTI sustained by Gram-+ bacteria, we observed a SE 0.68, SP 0.84. Conclusion: Our data although preliminary suggest that B_FSC could be useful in presumptive exclusion of UTI caused by Gram-positive bacteria. © 2014 Elsevier B.V. Source


Gessoni G.,Service of Clinical Pathology | Antico F.,Service of Clinical Pathology | Caroli D.,Madonna della Navicella Hospital | Nogara A.,Madonna della Navicella Hospital | And 4 more authors.
Minerva Endocrinologica | Year: 2013

In type 3 polyendocrine syndrome (PAS3), autoimmune thyroiditis occurs with other organ-specific autoimmune disease, but not with autoimmune adrenalitis. In this report we described a family from Pakistan in which mother and three daughters were affected by a PAS3. We studied a family from Pakistan: Father MMu age 44, mother KN aged 44, three daughters MM age 20, MH age 16 and MA age 14 and a son MU age 18. These subjects were tested for thyroids function, metabolic function, adrenal function, autoimmune disease. In this family the four females were shown hypothyroidism with presence of anti thyroid autoantibodies (AA) and high TSH serum concentration in association with the presence of anti transglutaminase AA. Moreover KN, MM and MH were positive for anti nuclear AA (granular pattern) and for antibodies against Saccaromyces cerdevisiae. MM was positive for AA against nuclear extractable antigens (SSA and SSB) too. No diabetes or pernicious anemia were observed. Adrenal and Pituitary function were normal. PAS 3C is an uncommon disease. In this family from Pakistan we observed a PAS3C in the four female members: mother and three daughters while father and son were unaffected. Source


Saccani G.,Clinical Chemistry and Microbiology Laboratory | Gessoni G.,Madonna della Navicella Hospital | Valverde S.,Madonna della Navicella Hospital | Manoni F.,Civil Hospital | Visconti M.,Clinical Chemistry and Microbiology Laboratory
Biochimica Clinica | Year: 2014

Urine culture is the most frequent test in microbiology laboratories. A screening tool, providing fast and reliable results to rule-out urinary tract infection (UTI), would be of great importance. We studied 1043 consecutive urine samples by Sysmex UF-1000i analyzer. Comparison was made by robotic urine culture on chromogenic agar with 1 μL loop, using 105 CFU/mL as a limit of positive growth. We evaluated bacteria quantification for rapid exclusion of UTI and bacteria forward scatter (B-FSC) in preliminary discrimination of UTI caused by Gram positive or Gram negative bacteria. For exclusion of UTI, the best cut-off value was 130 bacteria/μL. At this threshold, the sensitivity (SE) was 0.98 and the specificity (SP) 0.75. For exclusion of UTI sustained by Gram positive bacteria, the best cut-off value for B-FSC was 25ch. At this threshold, SE was 0.68 and SP was 0.89. Source

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