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Tehrān, Iran

Ganji S.M.,Iran National Institute of Genetic Engineering and Biotechnology | Sahebghadam-Lotfi A.,Iran National Institute of Genetic Engineering and Biotechnology | Sahebghadam-Lotfi A.,Tarbiat Modares University | Rastgar-Jazii F.,Iran National Institute of Genetic Engineering and Biotechnology | And 4 more authors.
Laboratory Medicine | Year: 2010

Background: Alpha-1 antitrypsin (AAT) deficiency is an inherited disease characterized by reduced levels of AAT in the serum. The 2 common genotypes of AAT deficiency are type Z (PiZ) and type S (PiS), which are associated with several malignancies. Methods: We assessed the AAT phenotype as well as genotypes Z and S in squamous cell carcinoma of the esophagus (SCCE) and their association with malignancy in Azeri patients of Iran. Alpha-1 antitrypsin phenotype identification was done using isoelectric focusing (IEF); its genotype was identified by RFLP. Results: The results indicated that the mean range of trypsin inhibitory capacity (TIC) and AAT nephlometry are significantly different in patients (P<0.05). In addition, while AAT levels are higher in the serum of patients they are not functional. This accounts for further dysfunction and reduction of its proper protease inhibitory activity in patients when compared with the healthy controls. Moreover, 97.3% of SCCE patients were homozygote for MM (PiMM), and only 2.7% were MS heterozygous. Neither of the PiZ and PiS genotypes were identified in the patients (P<0.05). Conclusion: Alpha-1 antitrypsin is a suitable prognostic rather than early stages diagnostic tumor marker in SCCE as its correlation with the disease could significantly be seen in the advanced stages of the esophageal tumorigenesis. As a tumor marker, AAT is highly sensitive but less tissue specific, thus its application is useful in combination with a panel of tumor markers in SCCE diagnosis, treatment, and follow up. Source

Sofian M.,Arak University of Medical Sciences | Aghakhani A.,Pasteur Institute of Iran | Farazi A.-A.,Arak University of Medical Sciences | Banifazl M.,Iranian Society for Support Patients With Infectious Diseases | And 8 more authors.
Travel Medicine and Infectious Disease | Year: 2010

Background: Hepatitis A is one of the most frequently reported vaccine-preventable diseases throughout the world and remains endemic in many areas. Studies in various communities have shown that Hepatitis A virus (HAV) prevalence rises with age. The current data regarding hepatitis A epidemiology in Iran is limited. The aim of this study was to determine the seroepidemiology of hepatitis A in children of different age groups in Tehran, Iran. Methods: Plasma samples of 1065 children between ages of 6 months and 20 years were tested for the presence of total anti-HAV. The study population was stratified according to age. Results: The prevalence of total anti-HAV was 61.6%. HAV prevalence rates according to age groups were as follows: 61.5% between 6 months and 1.9 years, 51.7% between 2 and 5.9 years, 52.9% between 6 and 10.9 years, 65.2% between 11 and 15.9 years, 85% between 16 and 20 years. Total anti-HAV seroprevalence was significantly different between age groups. Conclusion: The study findings indicate that hepatitis A is prevalent in children in Tehran, Iran and HAV infection is an important public health problem in this region. © 2010 Elsevier Ltd. All rights reserved. Source

Attaran-Bandarabadi F.,University of Tehran | Ziaee A.A.,University of Tehran | Yazdanbod M.,Madaen Hospital | Shahpanah M.,Delivery Room | And 2 more authors.
Genetics and Molecular Research | Year: 2011

There is a high incidence of esophageal squamous cell carcinoma (ESCC) in Iran. Non-functionality of some tumor suppressor genes has been reported in esophageal cancer. Loss of heterozygosity on chromosome 5 has also been reported in esophageal carcinomas. We assessed loss of heterozygosity along a region of the long arm of chromosome 5 (5q), from 5q23.1 to 5q23.2, by PCR amplifying DNA fragments of tumor tissues from patients with ESCC and their corresponding normal samples. The PCR products were electrophoresed on 6% non-denaturing polyacrylamide gels, and band intensity was shown by silver staining. Of 40 patients with ESCC, 27, 25 and 36% of informative cases showed allelic losses at microsatellite markers D5S1384, D5S1478 and D5S1505, respectively. Two of the 40 patients studied had microsatellite instability at marker D5S1384. Based on the fact that loss of heterozygosity with more than 22% incidence for a specific marker cannot be regarded as a random event, we add support to previous reports concerning the presence of tumor suppressor genes in this chromosome region and that they affect esophageal cancer development. According to the data in NCBI UniSTS, the PCR product size of human DNA with primers of the D5S1505 marker ranges from 243 to 275 bp, containing about 20 repeats of the TAGA tetranucleotide, while the amplicon size of one allele of one of our cases was 207 bp, with about 10 repeats of the TAGA tetranucleotide, which would be the shortest sequence reported so far. © FUNPEC-RP. Source

Eftekhary H.,University of Tehran | Ziaee A.-A.,University of Tehran | Yazdanbod M.,Madaen Hospital | Shahpanah M.,Bank Melli Iran Hospital | And 2 more authors.
Wspolczesna Onkologia | Year: 2015

Aim of the study: Matrix metalloproteinases (MMPs) are a zinc-dependant endopeptidase family that can degrade extracellular matrix components. Their dysregulation has been proven in several diseases, including cancer. Genetic variations in MMP promoter regions can alter their expression. The aim of the present study is to investigate the correlation of MMP-2 (-1306C/T), MMP-9 (-1562C/T), and MMP-12 (-82A/G) single nucleotide polymorphisms (SNPs) with oesophageal squamous cell carcinoma (ESCC) initiation and progression susceptibility in Iranian patients. Material and methods: MMP-2 (-1306C/T), MMP-9 (-1562C/T), and MMP-12 (-82A/G) SNPs were detected using polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) technique in 70 patients and 60 healthy controls. The genotypes and allele distributions were statistically compared in patients and controls. The correlation of MMP-2 (-1306C/T) and MMP-9 (-1562C/T) polymorphisms with clinicopathological features were investigated in 53 patients. Results: No statistically significant differences were observed in genotype and allele frequencies of MMP-2 (-1306C/T) and MMP-9 (-1562C/T) between patients and controls (p > 0.05). In addition, no relevance was observed in MMP-2 (-1306C/T) and MMP-9 (-1562C/T) SNPs and clinicopathological features. There was no nucleotide variation in MMP-12 (-82) in the case and control groups. Conclusions: This study indicates that these three SNPs may have no significant association in ESCC risk in Iranian patients. Source

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