MACS Agharkar Research Institute

Pune, India

MACS Agharkar Research Institute

Pune, India

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Karandikar K.G.,MACS Agharkar Research Institute | Singh S.K.,Kmc College
Mycotaxon | Year: 2010

A new hyphomycete species, Lylea indica, from Nagzira, Vidharba region of Maharashtra state in India found on dead culms of Bambusa arundinacea is here described.

Chitte R.R.,MACS Agharkar Research Institute | Deshmukh S.V.,MACS Agharkar Research Institute | Kanekar P.P.,MACS Agharkar Research Institute
Applied Biochemistry and Biotechnology | Year: 2011

Production of the fibrinolytic enzyme was carried out using 2.5-L glass fermentor, culture of thermophilic Streptomyces sp., and glucose yeast extract peptone medium of pH 8.0. Five successive batches were carried out under controlled fermentation conditions viz., agitation 140 rpm, aeration 0.5 vvm, 55 °C, and 18 h. The total protein extracellularly produced in the cell-free broth was ~300-500 mg/L. The enzyme belongs to serine endopeptidase type. Studies on the fibrin degradation indicate that the enzyme degrades the fibrin into small molecular weight products as seen from HPLC profile. Phase-contrast microscopic structure of fibrin showed that enzyme cleaves the fibrin filaments. The ex vivo activity of the actinokinase was compared with 500 IU of urokinase and 350 IU of streptokinase. The ex vivo clot lysis was found to be faster as compared to the commercial available enzymes. © 2011 Springer Science+Business Media, LLC.

Kulkarni S.O.,MACS Agharkar Research Institute | Kanekar P.P.,MACS Agharkar Research Institute | Jog J.P.,CSIR - National Chemical Laboratory | Patil P.A.,CSIR - National Chemical Laboratory | And 3 more authors.
Bioresource Technology | Year: 2011

Characterisation of polyhydroxyalkanoate (PHA) film produced by haloalkalitolerant Halomonas campisalis (MCM B-1027) in 14L SS fermenter revealed it to have composition of monomer units, HB:HV as 96:4 as analysed by 1H NMR indicating the PHA as a co-polymer of PHB-co-PHV, molecular weight by gel permeation chromatography as 2.08×106, melting temperature 166.51°C, tensile strength 18.8MPa; two relaxations namely beta transition corresponding to the glass rubber transition and alpha transition corresponding to crystalline relaxation by Dynamic Mechanical Thermal analysis and only one relaxation corresponding to MWS interfacial polarisation with activation energy of 129kJ/mol by broadband dielectric spectroscopy. Optical microscopic studies showed typical Maltese-cross pattern of spherulites. The PHA film was found to be biodegradable by standard ASTM method as well as by soil burial method. The leak proof polymer bags prepared from the film could be used as a packaging material. © 2011 Elsevier Ltd.

Mukherjee G.,MACS Agharkar Research Institute | Singh S.K.,MACS Agharkar Research Institute
Process Biochemistry | Year: 2011

A metabolite was purified from Monascus perpureus culture broth with the aim of isolating economically viable new pigment having food coloring and therapeutic potential. Following optimization of physical and nutritional parameters of submerged fermentation the pigment was extracted using hexane, and purified by thin layer chromatography (TLC), column chromatography and semi-preparative HPLC. Based on UV-vis, IR, GC-MS and NMR analyses the pigment was chemically characterized as 9-(1-hydroxyhexyl)-3-(2-hydroxypropyl)-6a- methyl-9,9a-dihydrofuro[2,3-h]isoquinoline-6,8(2H,6aH)-dione with the molecular mass of m/z 375. The pigment presents numerous similarities with two classical red pigments, rubropunctamine and monascorubramine, but differs by the presence of hydroxy alkane substitutions at C-3 and C-9 positions. Antibacterial plate assay showed its inhibitory activity against Gram-positive bacteria. These results suggest the purified pigment is a new red pigment from Monascus purpureus with potential application in food coloring and antibacterial therapy. © 2010 Elsevier Ltd. All rights reserved.

Krishnapati L.S.,MACS Agharkar Research Institute | Ghaskadbi S.,MACS Agharkar Research Institute
Microvascular research | Year: 2016

Cardiovascular and ischemic diseases are often associated with diabetes mellitus and develop due to occlusion of blood vessels leading to the blockage and insufficient blood supply to the target organs. Current therapeutic strategies for treating these pathologies include growth factor-, gene- and stem cell-based therapies. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2) have been used in clinical trials to induce blood vessels. On the other hand, increased levels of both these growth factors have been observed with intense insulin therapy in diabetes mellitus patients further leading to increased risk of retinopathy. This suggests the presence of a possible crosstalk between insulin, FGF and VEGF pathways during angiogenesis. In the present work, we report the likely absence of synergistic effect between insulin and FGF-2. This was initially observed at morphological and histological levels using chick embryonic chorioallantoic membrane (CAM) assay and confirmed by analyzing the expression of angiogenesis regulatory genes by semi-quantitative reverse transcriptase PCR (RT-PCR). Absence of combinatorial effect between insulin, FGF-2 and VEGF during angiogenesis was also demonstrated using CAM assay. Copyright © 2015 Elsevier Inc. All rights reserved.

Pandeeti E.V.P.,University of Hyderabad | Pitchika G.K.,University of Hyderabad | Jotshi J.,MACS Agharkar Research Institute | Nilegaonkar S.S.,MACS Agharkar Research Institute | And 2 more authors.
PLoS ONE | Year: 2011

Conventional leather processing involving depilation of animal hide by lime and sulphide treatment generates considerable amounts of chemical waste causing severe environmental pollution. Enzymatic depilation is an environmentally friendly process and has been considered to be a viable alternative to the chemical depilation process. We isolated an extracellular protease from Pseudomonas aeruginosa strain MCM B-327 with high depilation activity using buffalo hide as a substrate. This 33 kDa protease generated a peptide mass fingerprint and de novo sequence that matched perfectly with LasB (elastase), of Pseudomonas aeruginosa. In support of this data a lasB mutant of MCM B-327 strain lacked depilatory activity and failed to produce LasB. LasB heterologously over-produced and purified from Escherichia coli also exhibited high depilating activity. Moreover, reintroduction of the lasB gene to the P. aeruginosa lasB mutant via a knock-in strategy also successfully restored depilation activity thus confirming the role of LasB as the depilating enzyme. © 2011 Pandeeti et al.

Kulkarni S.O.,MACS Agharkar Research Institute | Kanekar P.P.,MACS Agharkar Research Institute | Nilegaonkar S.S.,MACS Agharkar Research Institute | Sarnaik S.S.,MACS Agharkar Research Institute | Jog J.P.,CSIR - National Chemical Laboratory
Bioresource Technology | Year: 2010

Several microorganisms produce polyhydroxyalkanoates (PHA). They are accumulated intracellularly as energy storage compounds. The PHAs are of interest because of their potential in biomedical applications. Halophilic bacteria and archaea are known to produce polyhydroxybutyrate (PHB). This paper describes production of a biodegradable copolymer, PHB-co-PHV by a moderately haloalkalitolerant Halomonas campisalis, isolated from Lonar Lake, India.The production of PHA was in the range of 45-81% on dry cell weight basis when the organism was grown in a production medium containing 1% (w/v) maltose and 0.1% (w/v) yeast extract, at pH ranging from 6 to 9 with an inoculum density of 105-107 cells/ml of medium, for incubation period of 15-30h and at 37°C. The polymer produced by the organism is a hydroxyester with molecular weight of 1.3014×106. Its melting temperature was 171°C. The 1H NMR analysis revealed that the polymer was a copolymer of PHB-co-PHV. This could be achieved by providing simple carbon source viz. maltose. © 2010 Elsevier Ltd.

Majumder D.R,MACS Agharkar Research Institute | Kanekar P.P,MACS Agharkar Research Institute
International Journal of Pharma and Bio Sciences | Year: 2012

Thermoactinomyces thalpophilus (Gene Bank accession number EF 397000) isolated from the waters of a thermal spring situated in Western Ghats, Maharashtra, India was found to be a potent producer of thermolysin-like protease (TLP). TLP was capable of bringing about condensation/ biotransformation of two amino acids, viz. Z-L-aspartic acid and L-phenylalanine methyl ester into aspartame, which is an artificial, nonsaccharide sweetener used as a sugar substitute in some foods and beverages. The maximum yield 181 U/ml/min was obtained at 55°C in glucose yeast extract medium(pH 7.2) containing CaCl2 (0.2 % w/v) with an inoculum size of 0.5% v/v after 18 hours incubation under shake-culture conditions (130 rpm).

Kasote D.M.,MACS Agharkar Research Institute | Hegde M.V.,Bharati Vidyapeeth Deemed University | Katyare S.S.,Bharati Vidyapeeth Deemed University
BioFactors | Year: 2013

Mitochondrial dysfunction is at the base of development and progression of several psychiatric and neurologic diseases with different etiologies. MtDNA/nDNA mutational damage, failure of endogenous antioxidant defenses, hormonal malfunction, altered membrane permeability, metabolic dysregulation, disruption of calcium buffering capacity and ageing have been found to be the root causes of mitochondrial dysfunction in psychatric and neurodegenerative diseases. However, the overall consequences of mitochondrial dysfunction are only limited to increase in oxidative/nitrosative stress and cellular energy crises. Thus far, extensive efforts have been made to improve mitochondrial function through specific cause-dependent antioxidant therapy. However, owing to complex genetic and interlinked causes of mitochondrial dysfunction, it has not been possible to achieve any common, unique supportive antioxidant therapeutic strategy for the treatment of psychiatric and neurologic diseases. Hence, we propose an antioxidant therapeutic strategy for management of consequences of mitochondrial dysfunction in psychiatric and neurologic diseases. It is expected that this will not only reduces oxidative stress, but also promote anaerobic energy production. © 2013 International Union of Biochemistry and Molecular Biology, Inc.

Rajeshkumar K.C.,MACS Agharkar Research Institute | Singh S.K.,MACS Agharkar Research Institute
Mycotaxon | Year: 2012

Manoharachariella indica is described as a new species from India, differing from M. lignicola, the only other described species of the genus, by larger conidia, longer conidiophores, and additional conidiophore septa. © 2012. Mycotaxon, Ltd.

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