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Kagawa ken, Japan

Kato M.,Hanakoganei Clinic | Goto S.,Chiyoda Corporation | Soma G.-I.,Tokushima Bunri University | Soma G.-I.,Macrophi Inc. | And 2 more authors.
Anticancer Research | Year: 2010

We describe the case of a patient with terminal adenocarcinoma of the lung with no response to lymphokine-activated killer (LAK) cell therapy alone who distinctly responded clinically to a combination of high-dose glucocorticoids (GC) and LAK cell therapy, administered by chance. However, after decreasing the dose of GC because of gastrointestinal bleeding caused by the high-dose treatment, there was no response on restarting GC plus LAK cell therapy. The clinical course of this case strongly suggests that local inflammation in the vicinity of tumors should be adequately suppressed in patients with advanced cancer who receive immunotherapy. Source

Wakame K.,Hokkaido University | Komatsu K.-I.,Hokkaido University | Inagawa H.,Kagawa University | Inagawa H.,Technology Research Inc | And 2 more authors.
Anticancer Research | Year: 2015

Background/Aim: Pantoea agglomerans LPS (immunopotentiator from Pantoea agglomerans 1: IP-PA1) has been reported to have anti-inflammatory effects in in vitro and in vivo models. The aim of the present study was to investigate the effects of orally-administered IP-PA1 on atopic dermatitis (AD) symptoms induced by Dermatophagoides farinae body extract (DFE) in NC/Nga mice. Materials and Methods: Using the NC/Nga AD murine model, mice were orally administered 0.1% (High) or 0.01% (Low) water-containing IP-PA1. Skin lesion assessment and blood collection from the caudal vein was performed on days 0, 7, 21 and 31. On day 31, all mice were sacrificed and blood, skin, spleen, as well as intestine samples, were obtained. Results: Assessment score of the skin lesion and serum immunoglobulin E (IgE) level of both IP-PA1 groups were significantly lower than that of the DFE group on days 14 and 21. The serum periostin and thymus and activation-regulated chemokine (TARC) level of IP-PA1-Low group was significantly lower than that of the DFE group on day 31. On histological examination of the skin, hyperplasia of epidermal and dermal layers and infiltration of inflammatory cells were suppressed by IP-PA1 administration. Deposition of periostin was observed in the DFE group skin tissue. Moreover, the CD4+/CD8+ ratio of splenic T-cells increased by IP-PA1 administration. Conclusion: IP-PA1 administration may have an inhibitory effect on AD skin lesions. Source

Kadowaki T.,Kagawa University | Inagawa H.,Kagawa University | Inagawa H.,Macrophi Inc. | Inagawa H.,Tokyo University of Science | And 8 more authors.
In Vivo | Year: 2011

Aims: Anti-lipopolysaccharide factor (ALF) is an antimicrobial peptide (AMP) and a key effector molecule of the innate immune system in crustaceans. However, little is known about the role of its indirect killing against bacteria. The possible regulatory role of this peptide (M-ALF) in kuruma prawns, Marsupenaeus japonicus, was investigated. Materials and Methods: The activities of M-ALF were investigated by antimicrobial activity in vitro and by experimental infection Vibrio penaeicida in vivo with ALF-knock down in kuruma prawn by systemically silencing M-ALF gene through the injection of gene-specific long double-stranded RNA with RNA interference. Results: Synthetic M-ALF had no direct antimicrobial activity against V. penaeicida, whereas ALF-silenced kuruma prawns had significantly higher mortality than untreated prawn after V. penaeicida infection. The data provide compelling evidence that M-ALF plays an indirect protective role against V. penaeicida infection, suggesting the idea that ALF acts as a cytokine-like regulatory molecule, as well as an effector molecule. Conclusion: M-ALF has no direct activity against V. penaeicida, but might be a key molecule in cytokine-like gene regulation in crustaceans. Source

Komori T.,Kohkin Chemical Co. | Saito K.,Kohkin Chemical Co. | Sawa N.,Forestry and Fisheries Technology Support Center | Shibasaki Y.,Nihon University | And 9 more authors.
Anticancer Research | Year: 2015

Background: Oral administration of lipopoly - saccharide (LPS), a major outer-membrane component of Gram-negative bacteria, has been found to prevent infection in mammals and fish. Oral administration of LPS is believed to increase phagocytic activity and promote cytokine production, thus associating it with priming. The present study aimed to elucidate the effect of oral administration of LPS in birds, which phylogenetically lie between fish and mammals, using chickens as a model. Materials and Methods: LPS derived from Pantoea agglomerans (LPSp) was added to the feed or water for oral administration to broiler chickens. For the survival assay and gene expression analysis (Genopal), LPSp was administered at a dose of 10 μg/kg of body weight (BW)/day. LPSp was administered at a dose of 0.2 μg/kg or 200 μg/kg to stimulate peripheral blood mononuclear phagocytosis (latex beads) and nitric oxide (NO) production. Results: LPSp (10 μg/kg BW/day) administration significantly inhibited mortality in broiler chickens on commercial farms. Furthermore, oral administration resulted in a transient increase in phagocytic activity and improved the ability to produce NO. On examining splenic cytokine induction following intraperitoneal administration of LPS derived from Escherichia coli (LPSe), we found significantly increased interleukin (IL)-1β mRNA expression. Conclusion: Innate immunity activation in chickens, as seen in infection prevention, was induced by oral administration of LPSp. This infection prevention involved increased phagocytic activity and enhanced gene expression and appears to be a phylogenetically-preserved innate immunity mechanism. Source

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