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Lu M.-Y.,Guilin Medical University | Ren Y.,The 303rd Hospital of PLA | Hu W.-Q.,Maanshan Municipal Peoples Hospital | Gui Y.,Guilin Medical University | Zhang L.-C.,The 303rd Hospital of PLA
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: The auditory ossicle chain reconstruction is still an important method to treat conductive deafness. Although a great variety of materials have been applied, the blood supply of otosteon after the implantation is ignored. Moreover, there is no real bone formed. OBJECTIVE: To observe the angiogenesis of vascular endothelial growth factor and collagen I modified β-tricalcium phosphate porous scaffold which is implanted into the otocyst of guinea pig. METHODS: Totally 60 guinea pigs were randomly divided into experimental group (vascular endothelial growth factor and collagen I modified β-tricalcium phosphate porous scaffold), collagen I control group (collagen I modified β-tricalcium phosphate porous scaffold) and blank control group (β-tricalcium phosphate porous scaffold). The guinea pigs were executed under anesthesia at weeks 1, 2, 3, 4 respectively. The surface of scaffolds was observed by scanning electron microscopy. The angiogenesis of scaffolds were observed by hematoxylin-eosin staining and CD34 immunohistochemistry staining, and then the microvascular density was counted. The osteogenesis of the scaffolds was observed by toluidine blue staining. RESULTS AND CONCLUSION: Endothelial cell proliferation and lumen formation could be observed after 1 week in the experimental group, and the angiogenesis reach the peak after 3 weeks with traffic branches formedbetween micropores. In the other two groups, the lumen formed at 2 weeks but no traffic branches were visible. The sprouting of new blood vessels in the pores were observed more in the experimental group than the other two groups (P < 0.05). The adherence and proliferation of cells could be examined in the surface and pores of the scaffold by scanning electron microscope. After 4 weeks, the osteogenesis could be observed by toluidine blue staining, especially in the experimental group. These findings suggest that the vascular endothelial growth factor and collagen I modified β-tricalcium phosphate porous scaffold can realize an effective vascularization in the environment of guinea pigs' middle ear. What's more, the scaffold also can promote bone formation. Source


Yin Z.,Nanjing Medical University | Yang X.,Nanjing Medical University | Jiang Y.,Nanjing Medical University | Xing L.,Maanshan Municipal Peoples Hospital | And 5 more authors.
Journal of Biomaterials Applications | Year: 2014

Objective: The purpose of this study was to determine whether the platelet-rich plasma-agarose gel scaffold could be a bioactive scaffold capable of growth factors release for cartilage repair. Methods: Porcine chondrocytes were seeded in agarose gel and platelet-rich plasma-agarose gel. During the 28-days culture, microstructure of hydrogels and morphologies of chondrocytes seeded in the hydrogels were observed using scanning electron microscope; viability of chondrocytes in gels was examined by live/dead assay; qualitative and quantitative analysis of glycosaminoglycan, collagen and DNA were assessed by histological, immunohistochemcial staining and biochemical assay; gene expression was measured by real-time polymerase chain reaction. In vitro cartilage ring models were used to evaluate the integration of the scaffolds, and the integration strength was analyzed by mechanical push-out tests. Results: Scanning electron microscope revealed both scaffolds had highly uniform porous structure. Live/dead scaffolds showed 100% cells alive in both groups. After 28-days culture, glycosaminoglycan, collagen, DNA content and chondrocyte- related genes expression in platelet-rich plasma-agarose gel were significantly higher than pure agarose gel. Integration strength in platelet-rich plasma-agarose gel was also higher compared to pure agarose gel. Conclusion: Platelet-rich plasma showed a positive effect on chondrocytes proliferation, differentiation and integration between native cartilage and engineered tissue when combined with agarose gel. Our findings suggest that platelet-rich plasma-agarose gel scaffold is a promising bioactive scaffold for future cartilage tissue engineering and future clinical works.© 2013 The Author(s). Source


Wei Z.,Anhui University of Technology | Yu X.,Anhui University of Technology | Xu X.,Anhui University of Technology | Chen X.,Maanshan Municipal Peoples Hospital
Computer Methods and Programs in Biomedicine | Year: 2014

In this paper, a new method involving an experiment in vivo and hydro-mechanical coupling simulations was proposed to investigate the biomechanical property of human periodontal ligament (PDL). Teeth were loaded and their displacements were measured in vivo. The finite element model of the experiment was built and hydro-mechanical coupling simulations were conducted to test some PDL's constitutive models. In the simulations, the linear elastic model, the hyperfoam model, and the Ogden model were assumed for the solid phase of the PDL coupled with a model of the fluid phase of the PDL. The displacements of the teeth derived from the simulations were compared with the experimental data to validate these constitutive models. The study shows that a proposed constitutive model of the PDL can be reliably tested by this method. Furthermore, the influence of species, areas, and the fluid volume ratio on PDL's mechanical property should be considered in the modeling and simulation of the mechanical property of the PDL. © 2013 Elsevier Ireland Ltd. Source


Chang L.,Nanjing Southeast University | Wu P.,Jiangsu Jiankang Vocational College | Senthilkumar R.,Nanjing Southeast University | Tian X.,Nanjing Southeast University | And 4 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2016

Purpose: Altered cellular metabolism has received increased attention as an important hallmark of cancer. Activation of FASN has been found to be involved in many human tumors. Despite extensive research in FASN function on cancer, the underlying mechanism is not entirely understood yet. Methods: Cerulenin was used to suppress the FASN expression in human colorectal cancer cell lines (HT29 and LoVo). Expression of PI3K, Akt, p-Akt, mTOR, p-mTOR, FASN, and AZGP1 was measured using western blotting and qPCR. ATP and lactic acid were assessed to investigate the activation of energy metabolism. Cell cytotoxicity assay was studied by cell counting kit-8 assay. The capacity of cell proliferation and migration was investigated by clonogenic and invasion assay. Analysis of apoptosis and the cell cycle was detected by flow cytometry. Results: We found that the expression of FASN was down-regulated, while the expression of PI3K, p-Akt, p-mTOR, and AZGP1 was down-regulated in HT29 and LoVo cells treated with FASN inhibitor. Proliferation was reduced in FASN inhibitor-treated cells, which is consistent with an increased apoptosis rate. Furthermore, the migration of FASN inhibitor-treated cells was decreased and the content of ATP and lactic acid was also dropped. Conclusion: These findings suggest that inhibited FASN suppresses the malignant phenotype of colorectal cancer cells by down-regulating energy metabolism and mTOR signaling pathway. The results have paved the way to understand the relations of FASN, mTOR signaling pathway, and energy metabolism in colorectal cancer cells. © 2015, Springer-Verlag Berlin Heidelberg. Source


Wang X.-Z.,Nanjing Medical University | Yang Z.-J.,Nanjing Medical University | Wang Y.-S.,Maanshan Municipal Peoples Hospital | Zhang P.,Maanshan Municipal Peoples Hospital | And 2 more authors.
Acta Academiae Medicinae Sinicae | Year: 2011

Objective: To evaluate the role of intracoronary electrocardiogram (IcECG) in examining early myocardial injury during percutaneous coronary intervention (PCI). Methods: Eight-six patients who had undergone elective PCI for their coronary heart disease were enrolled in the study. The IcECG both at baseline and after procedure were recorded with an incoronary guidewire and the serum levels of cardiac troponin T (cTnT) and creatine kinase-myoglobin were measured at baseline and 8 and 24 hours after intervention. Myocardial damage was defined as serum levels of cTnT increase above the upper normal value after intervention. Cardiac events after intervention was followed up. Results: Of all these 86 patients with normal serum levels of cardiac markers before the procedure, significant shift at ST-segment in IcECG during PCI was observed in 30 patients (35%, abnormal group) and no shift in the remaining 56 patients (65%, control group). All the procedures were successful. Serum levels of cTnT and creatine kinase-myoglobin were significantly higher in abnormal group than in control group after intervention (P < 0.01). The intracoronary ST-segment shift had a sensitivity of 77% and a specificity of 94% in predicting myocardial injury, with positive and negative predictive values of 90% and 86%, respectively. More cardiac events were observed in abnormal group than those in control group at a 4-week follow-up after intervention (P < 0.05) and major coronary event-free survival was significantly lower in those with post-procedural ST-segment shift in the IcECG (P < 0.05). Conclusion: IcECG may be a useful method for predicting myocardial injuries during PCI. Source

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