Jalandhar, India
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Mamik S.,Lyallpur Khalsa College | Sharma A.D.,Lyallpur Khalsa College
Russian Journal of Biological Invasions | Year: 2017

Lantana camara is an annual invasive weed of Verbenaceae family, which is native to tropical America while wide spread in North America, Africa, Australia and India. It tolerates a wide variety of abiotic conditions in the natural habitat. In the present study the abiotic stress-induced changes on reactive oxygen species (ROS) contents such as hydrogen peroxide (H2O2) and ROS scavenging boiling stable antioxidant proteins such as glutathione S-transferase (GST), glutathione reductase (GR), monodehydroascorbatereductase (MDAR), thioredoxin-reductase (TRx) and protein disulphide isomerise (PDI) were studied in three different most prevalent varieties of Lantana. The experiments were conducted from December-2013 to June- 2014. The study followed a random sampling method so that no bias is introduced. Three sites at random were selected and plant samples at reproductive stage (leaves and flowers) were collected from plants and pooled together for further analysis. Indices of oxidative stress, H2O2 level either increased or unchanged under low and high temperature conditions in a genotype and tissue dependent manner were determined. In Lantana the coordinated increase in the activities of various boiling stable antioxidant enzymes like GR, GST, PDI, TRx and MDAR was observed under adverse abiotic conditions in a genotype, and tissue specific manner. It was postulated that pink genotype may have more efficient mechanism to scavenge ROS species as shown by exorbitant increase in boiling stable antioxidant activities (BsGST, BsPDI, BsMDAR). Based on results it can be inferred that plants which are more invasive may have more biochemical capacity to perform biological antioxidative reactions to combat abiotic stress-induced oxidative stress. Overall, the present study findings can be used to control the invasiveness of invasive alien plants worldwide. © 2017, Pleiades Publishing, Ltd.


Kaur D.,Lyallpur Khalsa College | Kaur P.,Guru Nanak Dev University
Advances in Intelligent Systems and Computing | Year: 2017

The SQL injection is a predominant type of attack and threat to web applications. This attack attempts to subvert the relationship between a webpage and its supporting database. Due to widespread availability of valuable data and automated tools on web, attackers are motivated to launch high profile attacks on targeted websites. This paper is an effort to know the current state of SQL injection attacks. Different Researchers have proposed various solutions to address SQL injection problems. In this research work, those countermeasures are identified and applied to a vulnerable application and database system, then result are illustrated. © Springer Nature Singapore Pte Ltd. 2017.


Mamik S.,Lyallpur Khalsa College | Sharma A.D.,Lyallpur Khalsa College
Revista Brasileira de Botanica | Year: 2014

Alien plants have broad distribution throughout the world, in agriculture, forest,and natural areas displacing native vegetation, stunting or diminishing the growth, or development or establishment of native plants. Lantana is an annual invasive weeds of Verbenaceae (verbena) family, is native to tropical America while widespread in North America, many parts of Africa, Australia, and India. It tolerates a wide variety of abiotic conditions in the natural habitat. In order to gain insight into the biochemical basis of invasiveness of invasive alien species, in the present study, the abiotic stress condition-induced changes on reactive oxygen species contents (ROS) and ROS scavenging boiling stable antioxidant enzymes (peroxidase, superoxide dismutase (SOD), and catalase) were studied in three different most prevalent varieties of Lantana: pink (more invasive), red (moderate invasive), and violet (less invasive). Among all the enzymes studied, SOD was only detected as boiling stable. Indices of oxidative stress, superoxide radical and H2O2 content increased under low- and high-temperature conditions in a genotype- and tissue-dependent manner. For example, compared to other varieties, pink variety which is more invasive, showed higher ROS coupled with malondialdehyde contents under adverse abiotic conditions. As a result, it was postulated that pink genotype may have more efficient mechanism to scavenge ROS species as shown by the increase in boiling stable SOD(BsSOD) activity accompanied by enhanced accumulation of BsSOD isoenzymes. Based on the results, it can be inferred that plants which are more invasive may have more biochemical capacity to perform biological antioxidative reactions to combat abiotic stress-induced oxidative stress. © 2014, Botanical Society of Sao Paulo.


Neeraj,Lyallpur Khalsa College | Raina K.K.,Thapar University
Optical Materials | Year: 2013

With the advance of nanoparticles-liquid crystal composites, it became increasingly interesting to measure the various physical parameters, preferably near the transition temperature. In this research work, fine nickel nanoparticles with 2-8 nm size distribution were synthesized by chemical reduction of nickel hydrazine complex in aqueous solution at 60 °C. A small concentration of 0.5 wt.% of nickel nanoparticles dispersed in a novel ferroelectric liquid crystal mixture showed improved transmittance and fast switching response. Moreover, nickel addition indicates a slight increase in the conductivity of the ferroelectric sample. These studies indicate the occurrence of strong dipole interactions between metallic nanoparticles and ferroelectric liquid crystal molecules in the presence of applied electric field. © 2012 Elsevier B.V. All rights reserved.


Bedi C.,Lyallpur Khalsa College | Gill T.S.,Guru Nanak Dev University
Physics of Plasmas | Year: 2012

As is well known, the envelope electron acoustic (EA) nonlinear waves are expressed by nonlinear Schrödinger equation. In this paper, we find that under transverse perturbations, this kind of nonlinear waves can be described by Davey-Stewartson equation. In this work, modulational properties of EA wave and its stability regions in two-dimensional plasma have been studied. © 2012 American Institute of Physics.


Kaur D.,Lyallpur Khalsa College | Kaur P.,Guru Nanak Dev University
Procedia Computer Science | Year: 2016

The web applications are becoming more popular and complex in today's era of Internet. These on-line applications provide rich benefits along with risk to organization, brand and data. Malicious attackers continue to exploit vulnerabilities in applications in order to steal sensitive information. The outlines of this paper is to analyze web attacks in recent years that have compromised web applications, its data or its users. This paper includes the web attacks analysis from Website Hacking Incident Database (WHID) and other information security and news websites. Also, it is an effort to analyze various attacks on major categories of web sites which is a guide to developers to take respective appropriate preventive measures in future. The top web attacks have been identified and also the top vulnerable categories of web applications are analyzed. © 2016 The Authors.


Kaith B.S.,National Institute of Technology Jalandhar | Dhiman J.,National Institute of Technology Jalandhar | Bhatia J.K.,Lyallpur Khalsa College
RSC Advances | Year: 2015

Poly(HEMA) chain grafted chemically modified Holarrhena antidysenterica fiber was converted into a cation-exchanger using phosphorylation and a p-doping technique. The cation-exchanger synthesized was found to remove about 94.6% methylene blue dye from aqueous solution. The graft copolymer and cation exchanger vis-a-vis the backbone were characterized using different techniques like FTIR, SEM, EDX and XRD. The samples were further evaluated for thermal behavior using TGA/DTA/DTG techniques. The synthesized cation-exchanger was found to possess high thermal stability. © The Royal Society of Chemistry 2015.


Sharma A.D.,Lyallpur Khalsa College
Emirates Journal of Food and Agriculture | Year: 2010

The present study was designed to clone gene encoding phosphatase and its regulation by abiotic stress treatments. The genomic DNA was isolated, TaPase DNA was amplified and cloned from wheat (Triticum aestivum). Expression studies by northern blot analysis was carried out by isolating RNA from tissues under different abiotic stress treatments. Clustal-W analysis of TaPase (assession no EU. 723832) with all the reported phosphatase genes revealed that codon encoding tryptophan (TGG) residue was conserved. Northern blot analysis revealed that under abiotic stress treatments like osmotic, salt and heat, the expression of TaPase was induced, indicating modulation of TaPase under stress treatments. Based upon these results, a possible physiological role of TaPase in wheat is discussed.


Singh H.,CSIR - Central Electrochemical Research Institute | Singh H.,Lyallpur Khalsa College | Gahlan P.,CSIR - Central Electrochemical Research Institute | Kumar S.,CSIR - Central Electrochemical Research Institute
Gene | Year: 2013

Picrorhiza kurrooa Royle ex Benth. is an economically important medicinal plant known to yield picrosides which have high medicinal value. Picroside I and picroside II are major picrosides associated with various bioactivities. The present work analyzed the expression of various genes of the picrosides biosynthesis pathway in different tissues of the plant in relation to the picrosides content. Eight full-length cDNA sequences namely, 1-deoxy-d-xylulose-5-phosphate synthase (2.317. kb), 1-deoxy-d-xylulose-5-phosphate reductoisomerase (1.767. kb), 4-diphosphocytidyl-2-C-methyl-d-erythritol kinase (1.674. kb), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (1.701. kb), acetyl-CoA acetyltransferase (1.545. kb), 3-hydroxy-3-methylglutaryl coenzyme A reductase (2.241. kb), isopentenyl pyrophosphate isomerase (987. bp) and geranyl diphosphate synthase (1.434. kb), were cloned to full-length followed by expression analysis of ten genes vis-à-vis picrosides content analysis. There is maximum accumulation of picrosides in leaf tissue followed by the rhizome and root, and a similar pattern of expression was found in all the ten genes. The genes responded to the modulators of the picrosides biosynthesis. Picrosides accumulation was enhanced by application of hydrogen peroxide and abscisic acid, whereas methyl jasmonate and salicylic acid treatment decreased the content. © 2013 Elsevier B.V.


Ram G.,Lyallpur Khalsa College | Sharma A.D.,Lyallpur Khalsa College
International Journal of Bioinformatics Research and Applications | Year: 2013

MicroRNAs (miRNAs) are small endogenous genes regulators which regulate different processes underlying plant adaptation to abiotic stresses. To gain a deep understanding of role of miRNAs in plants, in the present study, we computationally analyzed different sorghum miRNAs in the drought-induced gene sequences. Homologous miRNA were identified using different plant miRNA databases. Using previously established genes databases, various targets of sorghum miRNAs were predicted viz: transcription factors, chaperonins, metabolic enzymes and other gene targets necessary for proper plant development. Analysis of cis-elements provides molecular evidence for the possible involvement of miRNAs in the process of abiotic stress tolerance in sorghum. Based on these results, it was suggested that miRNAs may play an important role in water stress tolerance. Copyright © 2013 Inderscience Enterprises Ltd.

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