Stasyuk N.Y.,Ukrainian Academy of Sciences |
Gayda G.Z.,Ukrainian Academy of Sciences |
Fayura L.R.,Ukrainian Academy of Sciences |
Boretskyy Y.R.,Ukrainian Academy of Sciences |
And 5 more authors.
Food Chemistry | Year: 2016
A highly selective and sensitive enzymatic method for the quantitative determination of l-arginine (Arg) has been developed. The method is based on the use of recombinant bacterial arginine deiminase (ADI) isolated from the cells of a recombinant strain Escherichia coli and o-phthalaldehyde (OPA) as a chemical reagent. Ammonia, the product of the enzymatic digestion of Arg by ADI, reacts with OPA and forms in the presence of sulfite a product, which can be detected by spectrophotometry (S) and fluorometry (F). The linear concentration range for Arg assay in the final reaction mixture varies for ADI-OPA-F variant of the method from 0.35 μM to 24 μM with the detection limit of 0.25 μM. For ADI-OPA-S variant of the assay, the linearity varies from 0.7 μM to 50 μM with the detection limit of 0.55 μM. The new method was tested on real samples of wines and juices. A high correlation (R = 0.978) was shown for the results obtained with the proposed and the reference enzymatic method. © 2016 Elsevier Ltd. All rights reserved.
Fedorovych D.,Ukrainian Academy of Sciences |
Boretsky Y.,Ukrainian Academy of Sciences |
Boretsky Y.,Lviv State University of Physical Culture |
Bobak Y.,Ukrainian Academy of Sciences |
And 3 more authors.
Cytology and Genetics | Year: 2015
Using similarity search we identified Candida (Pichia) guilliermondii genes involved in iron acquisition. This yeast possesses at least four genes potentially coding for ferrireductases, four genes encoding iron permeases and two genes coding for ferroxidases. Identified C.(P.) guilliermondii genes encoding ferroxidases possess different patterns of expression under iron repletion conditions whereas their expression is activated under iron deficiency conditions or in mutant strains defective in regulation of iron acquisition. C.(P.) guilliermondii has no homologue of Saccharomyces cerevisiae transcriptional regulator of iron metabolism, Aft1p and possess an iron regulatory network similar to that of Candida albicans. Since most of C.(P.) guilliermondii known strains are not pathogenic, in contrast to that of C. albicans, we propose C.(P.) guilliermondii as safe and useful model for studying iron-dependent regulation of metabolism in yeasts belonging to CUG clade. © 2015, Allerton Press, Inc.
Stasyk O.V.,Ukrainian Academy of Sciences |
Boretsky Y.R.,Ukrainian Academy of Sciences |
Boretsky Y.R.,Lviv State University of Physical Culture |
Gonchar M.V.,Ukrainian Academy of Sciences |
And 3 more authors.
Cell Biology International | Year: 2015
Tumor cells often exhibit specific metabolic defects due to the aberrations in oncogene-dependent regulatory and/or signaling pathways that distinguish them from normal cells. Among others, many malignant cells are deficient in biosynthesis of certain amino acids and concomitantly exhibit elevated sensitivity to deprivation of these amino acids. Although the underlying causes of such metabolic changes are still not fully understood, this feature of malignant cells is exploited in metabolic enzymotherapies based on single amino acid, e.g., arginine, deprivation. To achieve efficient arginine depletion in vivo, two recombinant enzymes, bacterial arginine deiminase and human arginase I have been evaluated and are undergoing further development. This review is aimed to summarize the current knowledge on the application of arginine-degrading enzymes as anticancer agents and as bioanalytical tools for arginine assays. The problems that have to be solved to optimize this therapy for clinical application are discussed. © 2014 International Federation for Cell Biology.
Zanevskyy I.,PulLaski Technical University |
Banakh V.,Lviv State University of Physical Culture
Acta of Bioengineering and Biomechanics | Year: 2010
A kinematical model of the ski jumper's body pose at the beginning of take-off was proposed. A method of measuring skier's body coordinates based on the results of video recordings and office information technologies was created. Kinematical parameters of the skier's body pose at the beginning of take-off were determined using sport competition results of 33 ski jumpers. Five parameters of the pose which show statistically significant correlation (p < 0.03) with the jump length were determined. A part of variation of the model parameters in the total variation of the jump length is almost equal to 53%, and relative correlation is strong and significant (p < 0.005). Recommendations regarding optimization of the body pose at the beginning of take-off were formulated.
Cherkas A.,Lviv State University of Physical Culture |
Golota S.,Private Laboratory Eurolab
Medical Hypotheses | Year: 2014
Glycogen storage in human organism is providing reserve source of glucose which is critical for normal functioning of the nervous system during periods between meals and is also important for many other tissues. Overwhelming excessive consumption of carbohydrates and decreasing physical activity among the world population lead to dramatic increase in incidence and mortality related to cardiovascular diseases, metabolic syndrome and diabetes mellitus type 2. There is an observation that many interventions with proved clinical efficiency like physical activity, intermittent fasting, caloric restriction and some pharmacological treatments have in common the ability to decrease content of glycogen in the liver and skeletal muscles. This effect leads to increased ability of these organs to uptake the next dose of glucose and store it in the form of glycogen. Moreover these interventions lead to significant life span extension, provide better body fitness and prevent development of multiple age-related diseases. In contrast excessive glucose load and saturation of tissues with glycogen provide a metabolic shift toward synthesis of fatty acids by liver. In advanced stages decreased glucose tolerance, insulin resistance, hyperinsulinemia, fatty liver disease, impairment of liver function and derangements of cholesterol metabolism are observed. It is suggested that noninvasive measurement of glycogen content in tissues could serve as important diagnostic and follow-up parameter for clinical practice and healthy lifestyle in wide population groups. © 2014 Elsevier Ltd.