Lviv State University of Physical Culture

ldufk.edu.ua/
L'viv, Ukraine

Lviv State University of Physical Culture is a Sports University in Lviv, Ukraine. Wikipedia.

SEARCH FILTERS
Time filter
Source Type

Zanevskyy I.,Kazimierz Pulaski Technological and Humanistic University | Zanevska L.,Lviv State University of Physical Culture
Journal of Testing and Evaluation | Year: 2016

A mean-score reliability of a test in a form of repeated trials with the same measurement equipment is estimated using an intraclass correlation coefficient varied within -1 and 1. The research aims to outline a method of estimation of the retest mean-score reliability coefficient adequate to its theoretical model, i.e., without negative values. The idea is: (1) to include all the scores obtained in the test-retest of the group of subjects to a united sample, and (2) to estimate corresponding true scores as individual means scores and include them to another sample. A proposed reliability score coefficient is derived as a ratio of a sum of squares of the individual means scores multiplied by a number of repeated trials to a total sum of squares. The method proposed in the work brings values of the mean-score reliability coefficient into the interval defined in the classic theory of test reliability: 0 to 1. The approach is illustrated with numerical examples. Copyright © 2015 by ASTM International.


Cherkas A.,Lviv State University of Physical Culture | Golota S.,Private Laboratory Eurolab
Medical Hypotheses | Year: 2014

Glycogen storage in human organism is providing reserve source of glucose which is critical for normal functioning of the nervous system during periods between meals and is also important for many other tissues. Overwhelming excessive consumption of carbohydrates and decreasing physical activity among the world population lead to dramatic increase in incidence and mortality related to cardiovascular diseases, metabolic syndrome and diabetes mellitus type 2. There is an observation that many interventions with proved clinical efficiency like physical activity, intermittent fasting, caloric restriction and some pharmacological treatments have in common the ability to decrease content of glycogen in the liver and skeletal muscles. This effect leads to increased ability of these organs to uptake the next dose of glucose and store it in the form of glycogen. Moreover these interventions lead to significant life span extension, provide better body fitness and prevent development of multiple age-related diseases. In contrast excessive glucose load and saturation of tissues with glycogen provide a metabolic shift toward synthesis of fatty acids by liver. In advanced stages decreased glucose tolerance, insulin resistance, hyperinsulinemia, fatty liver disease, impairment of liver function and derangements of cholesterol metabolism are observed. It is suggested that noninvasive measurement of glycogen content in tissues could serve as important diagnostic and follow-up parameter for clinical practice and healthy lifestyle in wide population groups. © 2014 Elsevier Ltd.


PubMed | Lviv State University of Physical Culture and Private Laboratory Eurolab
Type: Journal Article | Journal: Medical hypotheses | Year: 2014

Glycogen storage in human organism is providing reserve source of glucose which is critical for normal functioning of the nervous system during periods between meals and is also important for many other tissues. Overwhelming excessive consumption of carbohydrates and decreasing physical activity among the world population lead to dramatic increase in incidence and mortality related to cardiovascular diseases, metabolic syndrome and diabetes mellitus type 2. There is an observation that many interventions with proved clinical efficiency like physical activity, intermittent fasting, caloric restriction and some pharmacological treatments have in common the ability to decrease content of glycogen in the liver and skeletal muscles. This effect leads to increased ability of these organs to uptake the next dose of glucose and store it in the form of glycogen. Moreover these interventions lead to significant life span extension, provide better body fitness and prevent development of multiple age-related diseases. In contrast excessive glucose load and saturation of tissues with glycogen provide a metabolic shift toward synthesis of fatty acids by liver. In advanced stages decreased glucose tolerance, insulin resistance, hyperinsulinemia, fatty liver disease, impairment of liver function and derangements of cholesterol metabolism are observed. It is suggested that noninvasive measurement of glycogen content in tissues could serve as important diagnostic and follow-up parameter for clinical practice and healthy lifestyle in wide population groups.


PubMed | Lviv State University of Physical Culture and Loma Linda University
Type: Journal Article | Journal: Molecular therapy : the journal of the American Society of Gene Therapy | Year: 2015

Systemic 1,25(OH)2D3 treatment ameliorating murine inflammatory bowel diseases (IBD) could not be applied to patients because of hypercalcemia. We tested the hypothesis that increasing 1,25(OH)2D3 synthesis locally by targeting delivery of the 1-hydroxylase gene (CYP27B1) to the inflamed bowel would ameliorate IBD without causing hypercalcemia. Our targeting strategy is the use of CD11b(+)/Gr1(+) monocytes as the cell vehicle and a macrophage-specific promoter (Mac1) to control CYP27B1 expression. The CD11b(+)/Gr1(+) monocytes migrated initially to inflamed colon and some healthy tissues in dextran sulfate sodium (DSS) colitis mice; however, only the migration of monocytes to the inflamed colon was sustained. Adoptive transfer of Gr1(+) monocytes did not cause hepatic injury. Infusion of Mac1-CYP27B1-modified monocytes increased body weight gain, survival, and colon length, and expedited mucosal regeneration. Expression of pathogenic Th17 and Th1 cytokines (interleukin (IL)-17a and interferon (IFN)-) was decreased, while expression of protective Th2 cytokines (IL-5 and IL-13) was increased, by the treatment. This therapy also enhanced tight junction gene expression in the colon. No hypercalcemia occurred following this therapy. In conclusion, we have for the first time obtained proof-of-principle evidence for a novel monocyte-based adoptive CYP27B1 gene therapy using a mouse IBD model. This strategy could be developed into a novel therapy for IBD and other autoimmune diseases.


Bodnar I.,Lviv State University of Physical Culture | Prystupa E.,Lviv State University of Physical Culture
Human Movement | Year: 2015

Purpose. The aim of our investigation was to establish the efficiency of the integrated physical education classes for schoolchildren with minor deviations in health. Methods. Sociological (questionnaire), biomedical, pedagogical (testing), psycho-diagnostic, and mathematical statistics. Pupils (N = 1417) of secondary school (both sexes, 5th-9th forms, i.e. aged 10-15) were examined. Results. The characteristics of physical and mental development in the process of integrated physical education proved to be significantly better for different sex and age groups. Analysis of physical fitness characteristics shows that integrated physical education classes turned out to be more effective for schoolchildren with minor health deviations than for apparently healthy students. Integrated physical education classes are more effective for students' dexterity than for their power endurance. Integrated physical education classes are more effective for female students than for male ones; for schoolchildren with minor health deviations in older age groups (7th-9th forms) than for younger schoolchildren. Those schoolchildren who attended integrated physical education classes expressed significantly better attitudes to physical education than their peers who studied in a segregated environment. Conclusions. Integrated physical education classes are a more progressive and effective form of physical education in terms of their beneficial effect upon the physical and mental development of secondary school children with minor health distortions. © Human Movement 2016.


Stasyk O.V.,Ukrainian Academy of Sciences | Boretsky Y.R.,Ukrainian Academy of Sciences | Boretsky Y.R.,Lviv State University of Physical Culture | Gonchar M.V.,Ukrainian Academy of Sciences | And 3 more authors.
Cell Biology International | Year: 2015

Tumor cells often exhibit specific metabolic defects due to the aberrations in oncogene-dependent regulatory and/or signaling pathways that distinguish them from normal cells. Among others, many malignant cells are deficient in biosynthesis of certain amino acids and concomitantly exhibit elevated sensitivity to deprivation of these amino acids. Although the underlying causes of such metabolic changes are still not fully understood, this feature of malignant cells is exploited in metabolic enzymotherapies based on single amino acid, e.g., arginine, deprivation. To achieve efficient arginine depletion in vivo, two recombinant enzymes, bacterial arginine deiminase and human arginase I have been evaluated and are undergoing further development. This review is aimed to summarize the current knowledge on the application of arginine-degrading enzymes as anticancer agents and as bioanalytical tools for arginine assays. The problems that have to be solved to optimize this therapy for clinical application are discussed. © 2014 International Federation for Cell Biology.


Zanevskyy I.,Casimir Pulaski Technical University | Korostylova Y.,Lviv State University of Physical Culture | Mykhaylov V.,Lviv State University of Physical Culture
Human Movement | Year: 2012

Purpose. The purpose of this study was to elaborate on the scientific methodology for providing an assessment of air-pistol shooters' aiming stability by (i) substantiating the parameters of aiming stability, (ii) specifying the quantitative evaluation methods in using an optoelectronic target, (iii) evaluating the reliability of tests determining aiming stability, and (iv) developing a methodology for creating individual and group parameters of aiming stability. Methods. The aiming trajectories of 95 airpistol shooters, each of whom fired 60 shots using a SCATT optoelectronic simulator, were calculated to develop individual and group aiming stability parameters. Research methods included an optoelectronic registration of movement, research on top air-pistol shooters' sports results, mathematical modelling, variation statistics, cluster analysis, two-way ANOVA with data correlation and reliability theory tests. Results. It was found that an average aiming point trajectory on the SCATT optoelectronic target measured one second before a shot could be accepted as an assessment parameter of aiming stability, as it is one of the basic parameters of technical preparation and its position is not influenced by the ballistic characteristics of the pistol and pellets (η = 0.944). Conclusions. The parameters of aiming stability in air-pistol shooting were developed using a modification of the clustering method. This allows for the identification of weak points in the structure and organization of shooters' training and for adjustment of the training process.


Zanevskyy I.,Health Science University | Korostylova Y.,Lviv State University of Physical Culture | Mykhaylov V.,Lviv State University of Physical Culture
Proceedings of the Institution of Mechanical Engineers, Part P: Journal of Sports Engineering and Technology | Year: 2014

This article defines SCATT optoelectronic system accuracy of weapon movement registration. Three types of errors were found in defining the coordinates of the aiming point on a SCATT target: asymmetry of the left to right and up to down results, absolute errors in each direction and the dependence of the error upon the distance to the centre of the target. It was found that the optoelectronic registration shows the biggest errors in the left and up directions from the centre of the target (199%-283%) and relatively less errors in right (54%-188%) and down (18%-188%) directions. In order to make corrections while defining the aiming point coordinates on a SCATT target, one can use the correction factor based on the distance from the aiming point to the centre of the target. © IMechE 2014.


PubMed | Ukrainian Academy of Sciences and Lviv State University of Physical Culture
Type: | Journal: Food chemistry | Year: 2016

A highly selective and sensitive enzymatic method for the quantitative determination of L-arginine (Arg) has been developed. The method is based on the use of recombinant bacterial arginine deiminase (ADI) isolated from the cells of a recombinant strain Escherichia coli and o-phthalaldehyde (OPA) as a chemical reagent. Ammonia, the product of the enzymatic digestion of Arg by ADI, reacts with OPA and forms in the presence of sulfite a product, which can be detected by spectrophotometry (S) and fluorometry (F). The linear concentration range for Arg assay in the final reaction mixture varies for ADI-OPA-F variant of the method from 0.35 M to 24 M with the detection limit of 0.25 M. For ADI-OPA-S variant of the assay, the linearity varies from 0.7 M to 50 M with the detection limit of 0.55 M. The new method was tested on real samples of wines and juices. A high correlation (R=0.978) was shown for the results obtained with the proposed and the reference enzymatic method.


PubMed | Lviv State University of Physical Culture
Type: | Journal: Georgian medical news | Year: 2015

The aim of our study was to investigate frequencies of alleles and genotypes of Bcl1 GR gene polymorphism and their correlation with prevalence of BA. Study involved 188 patients with BA and 95 healthy individuals. Bcl1 (rs41423247) polymorphism in exon 2 was determined by means of polymerase chain reaction with subsequent RFLP analysis (restriction fragment length polymorphism) by Fleury I. et al. with modifications. Statistical analysis of the results was performed using SPSS-17 program. The results showed statistically significant differences in genotypes distribution of BclI polymorphism of GR gene in the control group and in patients with BA. The frequency of genotypes distribution of Bcl1 polymorphism of GR gene in controls: C/C, C/G, G/G - 0,421/ 0,453/0,126; in patients with bronchial asthma: 0.228/0.426/0.346, respectively (p=0.001). It was found out that G/G-homozygotes have a fivefold higher risk of BA than those homozygous for C/C regardless of gender. We demonstrated that BA risk in females, homozygous for the minor allele, is higher (p=0.016) and men with G/G genotype of Bcl1 GR gene polymorphism have the highest risk of BA. Thus, G/G genotype of Bcl1 polymorphism of the glucocorticoid receptor gene is associated with the development of asthma.

Loading Lviv State University of Physical Culture collaborators
Loading Lviv State University of Physical Culture collaborators