L'viv, Ukraine

Danylo Halytsky Lviv National Medical University , — formerly known as the Lvov State Medical Institute, earlier the Faculty of Medicine of the John Casimir University and, before that, Faculty of Medicine of the Francis I University — is one of the oldest and biggest medical universities in Ukraine.It is one of the leading medical universities of the IV level of accreditation. LNMU begins from the Medical Faculty of Lviv University, which was opened on November 16, 1784, according to the privilege of the Austrian emperor Josef II. In 2009 University celebrated its 225 anniversary. Wikipedia.

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Zhuraev R.,Lviv National Medical University
Cardiology Journal | Year: 2016

Background: The studies on heart rate variability (HRV), a key predictor of all-cause mortality, in Marfan syndrome (MS), up to now have not been reported, especially in patients with FBN1 mutations. Methods: Among 18 MS patients with the phenotype of MS meeting inclusion criteria 15 have had a FBN1 gene mutation. Short electrocardiography records were taken in the supine position and during orthostatic tests. The control group consisted of 30 apparently healthy nonathletes matched by age and gender. Results: Heart rates in MS patients with the FBN1 mutation were increased in both the supine position and orthostatic test (p < 0.001). Most of the time-domain (standard deviation, pNN50) and frequency-domain (total power, very low, low, and high frequency) parameters of HRV were significantly reduced in the MS patients (p < 0.001). Conclusions: A marked decrease in HRV, documented in the study, may be an important clinical feature in MS patients with confirmed FBN1 gene mutations. © 2016 Via Medica.

Kaminskyy D.V.,Lviv National Medical University | Lesyk R.B.,Lviv National Medical University
Biopolymers and Cell | Year: 2010

The aim of present research was investigation of anticancer activity of 4-azolidinone-3-carboxylic acids derivatives, and studies of structure-activity relationships (SAR) aspects. Methods. Organic synthesis; spectral methods; anticancer screening was performed according to the US NCI protocol (Developmental Therapeutic Program). Results. The data of new 4-thiazolidinone-3-alkanecarboxylic acids derivatives in vitro anticancer activity were described. The most active compounds which belong to 5-arylidene-2,4- thia(imida)zolidinone-3-alkanecarboxylic acids; 5-aryl(heteryl)idenerhodanine-3-succinic acids derivatives were selected. Determination of some SAR aspects which allowed to determine directions in lead-compounds structure optimization, as well as desirable molecular fragments for design of potential anticancer agents based on 4-azolidinone scaffold were performed. 5-Arylidenehydantoin-3-acetic acids amides were identified as a new class of significant selective antileukemic agents. Possible pharmacophore scaffold of 5-ylidenerhodanine-3-succinic acids derivatives was suggested. Conclusions. The series of active compounds with high anticancer activity and/or selectivity levels were selected. Some SAR aspects were determined and structure design directions were proposed. © Institute of Molecular Biology and Genetics NAS of Ukraine, 2010.

Lutsyk A.,Lviv National Medical University | Sogomonian E.,Lviv National Medical University
Folia Histochemica et Cytobiologica | Year: 2012

Twenty lectins with different carbohydrate affinities, including five new lectins purified from fungi, were used for histochemical examination of carbohydrate determinants in rat ovaries and endometrium under experimental hyper- and hypothyroidism. In the ovaries of control rats, lectin binding was detected predominantly in the zona pellucida and corona radiata of growing follicles, luteocytes, and leucocytes. Within the endometrium, lectins reacted strongly with the luminal surface, epitheliocytes and secretions of the uterine glands, pre-decidual cells, leucocytes, and collagen fibers. As revealed on general morphology and estrous cycle studies, hyperthyroidism induced ovarian luteinization, and increased the content of pre-decidual cells and of collagen fibers in the endometrium, with no reliable effect on the estrous cycle. Hypothyroidism was accompanied by retardation of the estrous cycle, enhanced folliculogenesis and leucocyte infiltration of ovarian and endometrial stroma. Both hyper- and hypothyroidism significantly affected the tissue carbohydrates of the ovaries and endometrium: specific and differential redistribution of DMan, LFuc, NeuNAc, DGlcNAc, DGalNAc, and DGal determinants was detected under both pathological conditions. Hyperthyroidism induced more severe alterations of glycoconjugates than hypothyroidism; the endometrium was more susceptible to thyroxin-modulated impairments than the ovaries. These results give new insights into the pathogenetic mechanisms of the effects of thyroid disorders on the female reproductive organs and demonstrate the suitability of lectin histochemistry methods for monitoring the efficacy of hormonal imbalance correction therapy, as well as the applicability of new lectin preparations for the selective labeling of ovarian and endometrial constituents. © Polish Society for Histochemistry and Cytochemistry Folia Histochem Cytobiol. 2012.

Nasadyuk C.,Lviv National Medical University | Sklyarov A.,Lviv National Medical University
Regulatory Peptides | Year: 2013

Background and objective: Some short peptides have recently been reported to exhibit gastroprotective properties but the role of NO-synthase system in these mechanisms still leaves much to be elucidated. That is why the purpose of our study was to explore the gastroprotective effect of the hexapeptide Arg-α-Asp-Lys-Val-Tyr-Arg (thymohexin) under conditions of the modeling of iNOS activity. Materials and methods: The studies were performed on 80 outbred male rats. Gastric lesions were induced with epinephrine (2. mg/kg) or indomethacin (30. mg/kg). Fifteen minutes before the exposure to ulcerogens rats were pretreated with thymohexin alone and combined with l-arginine or aminoguanidine. Twenty-four hours later gastric mucosa damage, l-arginine/NOS/NO system, processes of lipoperoxidation, superoxide dismutase and catalase activity were assessed. Results: Thymohexin markedly attenuated both epinephrine- and indomethacin-induced gastric ulceration in rats, decreasing the area and score of mucosal lesions (p<0.05), iNOS activity (p<0.05) and malonic dialdehyde content (p<0.05) in gastric mucosa. The cytoprotective effect of thymohexin was significantly enhanced by l-arginine and aminoguanidine. The combination of thymohexin and l-arginine was superior to that with aminoguanidine. Conclusions: Thymohexin protects gastric mucosa against epinephrine- and indomethacin-induced gastric lesions in rats. Thymohexin-induced gastroprotection is probably mediated by inhibition of iNOS and decrease of the oxidative damage in gastric mucosa. © 2012 Elsevier B.V.

Vynograd N.,Lviv National Medical University
Archivum Immunologiae et Therapiae Experimentalis | Year: 2014

The key aspects of the natural foci of especially dangerous diseases as a type of biological threats are presented. Approaches to epidemiological surveillance and control to the spread of the agents of especially dangerous diseases on endemic areas are described for zoonosis that has a medical value. The knowledge of specific design of tools for the implementation of epidemiological surveillance, monitoring and evaluation of natural foci diseases in developing countries is low; accordingly, little is known on the ecology and transmission dynamics for the agents of especially dangerous diseases. Important is to know the effectiveness of serological monitoring of the indigenous population to determine the activity of natural foci of hemorrhagic fever with renal syndrome, tick-borne encephalitis, tularemia, Q-fever, Lyme disease and West Nile disease. The main species of reservoirs and vectors for these agents have been determined in different regions of Ukraine. New tick-borne agents that were unknown for certain regions have been detected. These data indicate the spreading of different pathogens in combination with natural foci. © 2014, L. Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland.

Pohranychna Kh.,Lviv National Medical University
Experimental Oncology | Year: 2016

Aim: To study a rare clinical case of isolated primary osteoma localized in the frontal bone, provide its detailed clinical and pathomorphological analysis, and evaluate statistical indicators of occurrence frequency and localization of facial skeleton osteomas during 2006-2015. Materials and Methods: The retrospective case records analysis of patients undergoing treatment in the maxillofacial department of Lviv Regional Clinical Hospital and suffering from facial skeleton osteomas was conducted. The clinical examination was carried out in accordance with the inspection protocol required for the examination of patients diagnosed with tumors and tumor-like neoplasms in a particular clinical case. Frontal and lateral views were used in the course of X-ray imaging. Histological studies included macroscopy and microscopy. Results: According to the retrospective case record analysis made over the last 10 years 346 cases of facial bone osteoma were revealed and proved histologically comprising 3.7% of all benign osteogeneous maxillofacial tumors. For the first time in 10 years osteoma localized in the frontal bone had been revealed and thoroughly studied. Macroscopically-a semi-spherical and immovable neoformation 7 cm in diameter of hard consistency, splaying out at 4 cm and being adherent with the frontal bone. Roentgenologically-homogeneous intense shadowing of a round form with relatively distinct outlines complying with clinical sizes. According to the histological data, the tumor consists mostly of the solid osseous tissue and the spongy osseous tissue with the evident thickened trabeculas of the bone located in different directions. Conclusion: The analysis of historical data demonstrated extremely rare localization of primary osteoma of the frontal bone, and indicated the uniqueness of the case and significant clinical importance of its detailed study. Copyright © Experimental Oncology, 2016.

Besedina A.,Lviv National Medical University
Journal of Medical Biochemistry | Year: 2016

Background: Coronary heart disease is the leading cause of death and disability worldwide. Hypertension is a major independent risk factor for the development of CHD. Abnormalities in NO generation or activity have been proposed as a major mechanism of CHD. The purpose of this article is to determine the activity of eNOS and iNOS in patients with isolated CHD and CHD associated with HT of different age groups. Methods: Fifty patients with isolated CHD and 42 patients with CHD associated with HT were enrolled in this study. NOS activity was determined by nitrite anion formed in the reaction. Results: A statistically significant increase in iNOS activity is observed in elderly donors. In patients with isolated coronary heart disease cNOS activity is statistically significantly reduced with respect to the control group. The reduction of enzymatic activity of cNOS is more expressed in elderly patients than in middle-aged patients with coronary heart disease. Alterations in eNOS activity are more expressed in patients with coronary heart disease associated with hypertension than in patients with isolated coronary heart disease. Against the background of cNOS inhibition in the patients, a sharp increase in iNOS activity is observed. Conclusions: It has been shown that disturbance of endo - thelial function in patients with coronary heart disease associated with hypertension is characterized by reduced endothelial NO synthesis by cNOS and increased systemic NO synthesis due to increased iNOS activity. It has been found that the lack of endothelial NO and hyperproduction of "harmful" NO by iNOS are more expressed in elderly patients. © by Anna Besedina 2016.

Gerasimov S.V.,Lviv National Medical University | Vasjuta V.V.,Lviv National Medical University | Myhovych O.O.,Private Pediatric Clinic Vita Pueri | Bondarchuk L.I.,Lviv State Dermatology Hospital
American Journal of Clinical Dermatology | Year: 2010

Background: The role of probiotics in the treatment of atopic dermatitis (AD) remains controversial. A recent systematic review of the available evidence called for further clinical trials with new probiotic formulations. Objective: To assess the clinical efficacy and impact of Lactobacillus acidophilus DDS-1, Bifidobacterium lactis UABLA-12 with fructo-oligosaccharide on peripheral blood lymphocyte subsets in preschool children with moderate-to-severe AD. Method: Randomized, double-blind, placebo-controlled, prospective trial of 90 children aged 1-3 years with moderate-to-severe AD who were treated with a mixture of L. acidophilus DDS-1, B. lactis UABLA-12 with fructo-oligosaccharide at a dosage of 5 billion colony-forming units twice daily for 8 weeks versus placebo. The primary outcome measure was the percentage change in Scoring of Atopic Dermatitis (SCORAD) value. Other outcome measures were changes in Infant Dermatitis Quality Of Life (IDQOL) and Dermatitis Family Impact (DFI) scores, frequency and amount of topical corticosteroid used, and lymphocyte subsets in peripheral blood measured by laser flow cytometry. Results: At the final visit, the percentage decrease in SCORAD was 33.7% in the probiotic group compared with 19.4% in the placebo group (p = 0.001). Children receiving probiotic showed a greater decrease in the mean [SD] SCORAD score than did children from the placebo group at week 8 (-14.2 [9.9] vs -7.8 [7.7], respectively; p = 0.001). IDQOL and DFI scores decreased significantly from baseline by 33.0%and 35.2%in the probiotic group and by 19.0% and 23.8% in the placebo group, respectively (p = 0.013, p = 0.010). Use of topical corticosteroids during the 8-week trial period averaged 7.7 g less in probiotic patients (p = 0.006). CD3, CD16, and CD22 lymphocyte subsets remained unchanged, whereas the percentage of CD4, and the percentage and absolute count of CD25 decreased, and the percentage and absolute count of CD8 increased in the probiotic group at week 8 (p < 0.007 vs placebo). There was a significant correlation between CD4 percentage, CD25 percentage, CD25 absolute count, and SCORAD values (r = 0.642, r = 0.746, r = 0.733, respectively; p < 0.05) in the probiotic group at week 8. Conclusion: The administration of a probiotic mixture containing L. acidophilus DDS-1, B. lactis UABLA- 12, and fructo-oligosaccharide was associated with significant clinical improvement in children with AD, with corresponding lymphocyte subset changes in peripheral blood. The efficacy of probiotic therapy in adults with AD requires further investigation. © 2010 Adis Data Information BV. All rights reserved.

Zayachkivska O.,Lviv National Medical University
Frontiers of Gastrointestinal Research | Year: 2012

Esophageal inflammation is the highly complex protective response to cellular/tissue injury, one of the fundamental features in gastro-esophageal reflux disease (GERD) that represents a wide group of acid-related disorders, where a majority of patients appear to have nonerosive reflux disease (NERD) with minimal change esophagitis and no endoscopic abnormalities. Despite recent advances and better understanding of the physiopathogenesis of GERD and NERD, the molecular events of inflammation leading to erosive esophagitis (EE) and nonerosive esophagitis (NEE) development, their delay in healing and recurrence remains unclear. Membrane and integral glycoconjugate transformation of different cells in the esophageal barrier (EB) is associated with the reprogramming of pathways that control inflammation, survival and proliferation. The focus of this review is to summarize our data on the bidirectional relationship between the glycoconjugates - variable mediators for structural, modality roles and inflammatory settings in esophageal disorders. We designed and carried out experimental studies that induced esophageal damage, mimicking the esophageal injury of human GERD and NERD. We examined it using functional, morphologic and molecular biologic tests, and which and if pathways of inflammation precede changes in EB and development of mucosal lesions during the early stages of NEE or EE. We showed that glycoconjugates operate as tags for esophageal mucosal inflammation, ulceration and control communication between cell populations in repair. Detailed characterization of sialoglycans and their dynamics offers insights into functional interplay in the esophageal cellular community, whereas they switch the pro-inflammatory events involved in early nonepithelial cell activation and mucosal restitution. Esophageal mucosal repair and ulcer healing through different responses cannot be separated from changes in glycoconjugates upon modulation activity of limited and localized inflammation, cytotoxic nitrogen effects, and oxidative injury of epithelial and endothelial cells, cell proliferation, and migration. This new approach to glycobiology will provide an innovative view on inflammation in the physiopathogenesis of NERD and GERD. Copyright © 2012 S. Karger AG, Basel.

Urbanovych A.,Lviv National Medical University
Current Issues in Pharmacy and Medical Sciences | Year: 2015

Resistin is still a little known hormone of the adipose tissue. The potential role of resistin in the development of DM2 has been currently investigated. The aim of our study was to detect the resistin blood level in patients with DM2, depending on the duration of the disease. In so doing, a determination of resistin and insulin blood level was conducted in 305 patients with DM2, and in a control group of 32 persons. Before testing, the patients were placed into four groups, depending on the duration of type 2 diabetes. Our results indicate that the resistin level was significantly lower in the control group of patients, in comparison with the DM2 patients groups. Moreover, a significantly lower resistin level was found in group I (firstly diagnosed DM2), in comparison with the groups of patients with a different duration of DM2. No correlation between resistin level and BMI, and between resistin and insulin blood level was found. However, a tendency towards increase of resistin blood level is noticeably evident in co-relation with increment of DM2 duration. In addition, the resistin level was considerably lower in patients with no DM2, when compared with patients with diagnosed DM2. Yet, there was no significant difference in the resistin blood level depending on the sex of the patients at the same duration of DM2. © 2015 Medical University of Lublin. All rights reserved.

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