Luoyang Orthopedic Traumatological Hospital of Henan Province

Luoyang, China

Luoyang Orthopedic Traumatological Hospital of Henan Province

Luoyang, China
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Feng W.-H.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Zhang C.-X.,Wenzhou University | Zhang Y.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Li H.-T.,Luoyang Orthopedic Traumatological Hospital of Henan Province | And 2 more authors.
Latin American Journal of Pharmacy | Year: 2014

In this study, a simple, rapid and sensitive high performance liquid chromatography (HPLC) method is developed for determination of ulifloxacin (ULF) in human plasma samples using fleroxacin as the internal standard (IS). Sample preparation was accomplished through one-step protein precipitation with 35% perchloric acid, and chromatographic separation was carried out on an Agilent Zorbax Eclipse XDB-C18 (4.6 × 150 mm, 5) at 25 °C. Mobile phase composed of a mixture of acetonitrile-2% glacial acetic acid-water (20:40:40) at flow rate of 0.8 mL/min. Wavelength was set at 278 nm. The chromatographic retention times of ULF and IS were 5.46 and 4.45 min, respectively. The lower limit of quantitation (LLOQ) was 25 ng/mL, and no interferences were detected in the chromatograms. The devised HPLC method was validated by evaluating its intra- and inter-day precisions and accuracies in a linear concentration range between 25 and 5000 ng/mL. The method was successfully applied to a pharmacokinetic study of oral PLF tables in Chinese healthy volunteers.


Feng W.-H.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Zhang H.-H.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Zhang Y.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Sun M.,Henan University of Science and Technology | Niu J.-L.,Henan University of Science and Technology
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2015

In this study, a simple, sensitive, and robust analytical method based on ultra-performance liquid chromatography (UPLC) has been developed for the determination of galangin in rat plasma using diazepam as internal standard (IS). After sample preparation by a simple liquid-liquid extraction, chromatography was performed on an Acquity UPLC BEH C18 column (2.1. mm. ×. 50. mm, 1.7. μm particle size) and ultraviolet detection set at a wavelength of 360. nm. The method was linear over the concentration range 10-1000. ng/mL with a lower limit of quantification (LLOQ) of 10. ng/mL. Inter- and intra-day precision (RSD %) were all within 9.5% and the accuracy (RE %) was equal or lower than 8.9%. Recoveries of galangin and IS were more than 78.3%. Stability studies showed that galangin was stable under a variety of storage conditions. The method was successfully applied to a pharmacokinetic study involving oral administration of galangin to rats. © 2015 Elsevier B.V.


Lv S.-F.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Wang X.-H.,Henan University of Science and Technology | Li H.-W.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Zhang X.-L.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Wang B.,Luoyang Orthopedic Traumatological Hospital of Henan Province
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2015

An ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine liensinine in rat plasma using carbamazepine as the internal standard (IS). Sample preparation was accomplished through a protein precipitation procedure with acetonitrile to 0.1ml plasma sample. The analyte and IS were separated on an Acquity UPLC BEH C18 column (2.1mm×50mm, 1.7μm) with the mobile phase of acetonitrile and 0.1% formic acid in water with gradient elution at a flow rate of 0.40ml/min. The injection volume was 6μl. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization (ESI) by multiple reactions monitoring (MRM) of the transitions at m/z 611.6→206.2 for liensinine and m/z 237.1→194.2 for IS. The linearity of this method was found to be within the concentration range of 10-1000ng/ml with a lower limit of quantification of 10ng/ml. Only 3.0min was needed for an analytical run. The matrix effect was 93.8-107.4% for liensinine. The intra- and inter-day precision (RSD %) were less than 9.9% and accuracy (RE %) was within ±10.5%. The recovery ranged from 76.2 to 86.8%. Liensinine was sufficiently stable under all relevant analytical conditions. The method was also successfully applied to the pharmacokinetic study of liensinine in rats. The pharmacokinetic parameters were demonstrated as followed: t1/2 was 8.2±3.3h, Cmax was 668.4±156.9ng/ml, and AUC0→∞ was 1802.9±466.4ng/mlh. © 2015 Elsevier B.V.


Jin G.,Luoyang Orthopedic Traumatological Hospital of Henan Province
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2010

To study the treatment method and effect of abduction and lateral rotation limitation of the shoulder in obstetric brachial plexus palsy (OBPP). From February 2005 to August 2008, 11 patients with abduction and lateral rotation limitation of the shoulder in OBPP were treated with dissection of the origin of subscapular muscle, transfer of the tendons of latissimus dorsi and teres major muscle to the tendons of supraspinous and infraspinous muscles. Among them, there were 6 males and 5 females with a mean age of 6 years (1-15 years). The main clinical manifestations showed adduction, internal rotation contracture deformity of shoulder, limited active and passive external rotation and severely restricted active abduction of shoulder. The passive abduction was more than 90 degrees. According to Gilbert grading, there were 7 cases of grade 1 and 4 cases of grade 2. Based on Mallet score systems, the scores were 5 points in 3 cases, 6 points in 3 cases, and 7 points in 5 cases. The muscle strength of deltoid, supraspinatus, infraspinatus, teres major muscle and latissimus dorsi all reached 3-4 grades. One patient developed postoperative hematoma, wound healed after symptomatic management. Other patients achieved incision healing by first intention. All patients were followed up for 12 to 37 months (17 months on average). The active abduction and external rotation of the shoulder joints recovered obviously. The Gilbert grading were grade 2 in 1 case, grade 3 in 1 case, and grade 4 in 9 cases; the Mallet scores were 10 points in 1 case, 11 points in 2 cases, 12 points in 4 cases, 13 points in 3 cases, and 14 points in 1 case; showing significant differences when compared with those before operation (P < 0.01). The muscle strength of deltoid, supraspinatus, infraspinatus, teres major muscle and latissimus dorsi increased to 4-5 grades. The dissection of the origin of subscapular muscle, transfer of the tendons of latissimus dorsi and teres major muscle to the tendons of supraspinous and infraspinous muscles can resolve shoulder adduction, internal rotation contracture, and can enhance abduction, external rotation strength. It is an effective operation for abduction and lateral rotation limitation of the shoulder in OBPP.


Yuan Y.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Huang X.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Zhang Y.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Wang Z.,Luoyang Orthopedic Traumatological Hospital of Henan Province
International Journal of Clinical and Experimental Medicine | Year: 2015

Aims: The present study aims to investigate the clinical outcomes of arthroscopic suture fixation in treating tibial eminence fracture with a retrospective study design of two years’ follow-up. Methods: A total of 33 patients with imaging evidence of tibial eminence avulsion fractures who underwent arthroscopic surgery between 2008 and 2012 were included in this study. The inclusion criteria for the study were a displaced tibial eminence avulsion fracture and anterior knee instability of grade II or higher inskeletally mature patients. These patients were treated with arthroscopic suture fixation and followed with a mean period of 24 months. Anteroposterior and lateral radiographs were obtained 3 months postoperatively to assess fracture healing. At 24 months after surgery, all patients were evaluated by an independent orthopaedic professor with clinical examination like anteroposterior laxity (Lachman-Noulis and anterior drawer tests) and Rolimeter knee tester (Aircast, Vista, CA). Knee range of motion was evaluated actively and passively with a goniometer. Knee function was evaluated by the Lysholm and International Knee Documentation Committee (IKDC) scores. Knee radiographs in standing anteroposterior, standing lateral, and Merchant views were examined for alignment, joint space narrowing, and degenerative knee changes. Results: No major complication like infection, deep venous thrombosis, or neurovascular deficit happened peri-operatively. At the final follow-up, there were no symptoms of instability and no clinical signs of ACL deficiency. Radiographs showed that all fractures healed 3 months post-operative, but at the last follow-up, there was one person with degenerative changes like joint space narrowing in radiographs. Anterior translation of the tibia was 0.47 mm on average (0 to 2.5 mm) compared with the uninjured side. Range-of-motion measurement showed a mean extension deficit of 1.5° (0° to 5°) and a mean flexion deficit of 2.7° (0° to 10°) compared with the unaffected side. The mean Lysholm score was 96 (85 to 100), and the mean IKDC score was 94 (80 to 100). Overall, the IKDC grade was A (normal) in 24 patients (58%), B (nearly normal) in 8 patients (33%), and C (abnormal) in 1 patient (8%). Conclusion: The present study demonstrated tibial eminence fractures in adults can be effectively treated with arthroscopic suture fixation. © 2015 E-Century Publishing Corporation. All rights reserved.


Zhao A.-Q.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Zhao J.-H.,Henan University of Science and Technology | Zhang S.-Q.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Pan Y.-Y.,Henan University of Science and Technology | Huo X.-L.,Henan University of Science and Technology
Journal of Pharmaceutical and Biomedical Analysis | Year: 2016

A rapid, sensitive and selective ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the determination and pharmacokinetic investigation of parthenolide in rat plasma. Sample preparation was accomplished through a simple one-step deproteinization procedure with 0.2. mL of acetonitrile containing 30 ng/mL of pirfenidone (IS), and to a 0.1. mL plasma sample. Plasma samples were separated by UPLC on an Acquity UPLC BEH C18 column using a mobile phase consisting of acetonitrile-0.1% formic acid in water with gradient elution. The total run time was 3.0. min and the elution of parthenolide was at 1.33. min. The detection was performed on a triple quadrupole tandem mass spectrometer in the multiple reaction-monitoring (MRM) mode using the respective transitions m/. z 249.2. →. 231.1 for parthenolide and m/. z 186.2. →. 92.1 for pirfenidone (IS), respectively. The calibration curve was linear over the range of 2.0-500. ng/mL with a lower limit of quantitation (LLOQ) of 2.0. ng/mL. Mean recovery of parthenolide in plasma was in the range of 78.2-86.6%. Intra-day and inter-day precision were both <8.3%. This method was successfully applied in pharmacokinetic study after oral and intravenous administration of parthenolide in rats. © 2015 Elsevier B.V.


Jin G.Q.,Luoyang Orthopedic Traumatological Hospital of Henan Province
Zhongguo gu shang = China journal of orthopaedics and traumatology | Year: 2012

To introduce the method of the transverse carpal ligament (TCL) transection through palmar mini-incision for carpal tunnel syndrome (CTS) and evaluate its efficacy and safety. From January 2006 to September 2007, 15 patients (18 hands) with CTS were treated with palmar longitudinal mini-incision decompression. There were 2 males and 13 females, with an average age of 48 years (ranged 34 to 69) and an average course of 18 months (ranged 8 to 26). The main clinical symptom including the radial numbness or pain of the 3 and a half fingers, wrist pain and radiation to the forearm, the night awake history of numbness, thenar muscle atrophy, positive Tinel sign and Phalen sign. Median nerve electrophysiology showed that sensory nerve conduction velocity (SCV) slowed down and sensory nerve action potential (SNAP) decreased or missed, short abductor muscle of thumb had spontaneous potential in severe cases. Clinical effect were evaluated according to the Global symptom score (GSS) scoring in aspect of pain, numbness, paraesthesia, weakness and nocturnal awakening. Postoperative wound smoothly healed in all patients and no complications occurred. All patients were followed up from 20 to 28 months with an average of 24 months. Symptoms of 1 patient had not incomplete relief, other patient's symptoms disappeared and muscle force of abductor pollicis brevis reinforced. Postoperative GSS scoring obviously improved than preoperative (P < 0.05). Treatment of carpal tunnel syndrome through palmar mini-incision decompression has advantages such as higher safe, shorter operative time, less invasive, smaller scar, which can cut off carpi transversum ligament and thoroughly decompress median nerve under direct sight, it is a safe and effective operative approach.


Jin G.Q.,Luoyang Orthopedic Traumatological Hospital of Henan Province
Zhongguo gu shang = China journal of orthopaedics and traumatology | Year: 2012

To introduce treatment method of replantation of severed thumb trauma with proximal arterial, and to evaluate its efficacy. From February 2007 to March 2009,13 patients with severed thumb in serious injury of proximal arterial were treated with vein graft of volar forearm,bridging between dorsal carpal branch of radial artery on nasopharyngeal fossa and distal stump of ulnar proper digital artery. Among them, there were 11 males and 2 females with an average age of 34.5 years ranging from 16 to 50 years. Seven cases were in the left thumb,6 in the right thumb. Eight cases were complete separation, incomplete separation in 5 cases. Ten cases recieved emergency reimplantation, 3 cases with the arterial crisis after conventional replantation were explorated and repaired. Thumb of 13 cases all survived. All patients were followed-up for 8 to 17 months (averaged, 11 months). Replantation thumb obtained satisfactory appearance. According to Chinese Medical Association Society of Hand Surgery Trial criteria, the results were excellent in 9 fingers, good in 3 fingers, 1 poor finger. Two point discrimination of finger pulp was 5 to 8 mm (averaged 6.5 mm). By repairing artery of thumb with vein graft of volar forearm, bridging between dorsal carpal branch of radial artery on nasopharyngeal fossa and distal stump of ulnar proper digital artery,complex severed thumb replants on surgery position comfortable,without affecting the blood supply of the hand, expanding the indications for replantation and improving the success rate of replantation.


PubMed | Luoyang Orthopedic Traumatological Hospital of Henan Province
Type: Journal Article | Journal: International journal of clinical and experimental medicine | Year: 2015

The present study aims to investigate the clinical outcomes of arthroscopic suture fixation in treating tibial eminence fracture with a retrospective study design of two years follow-up.A total of 33 patients with imaging evidence of tibial eminence avulsion fractures who underwent arthroscopic surgery between 2008 and 2012 were included in this study. The inclusion criteria for the study were a displaced tibial eminence avulsion fracture and anterior knee instability of grade II or higher inskeletally mature patients. These patients were treated with arthroscopic suture fixation and followed with a mean period of 24 months. Anteroposterior and lateral radiographs were obtained 3 months postoperatively to assess fracture healing. At 24 months after surgery, all patients were evaluated by an independent orthopaedic professor with clinical examination like anteroposterior laxity (Lachman-Noulis and anterior drawer tests) and Rolimeter knee tester (Aircast, Vista, CA). Knee range of motion was evaluated actively and passively with a goniometer. Knee function was evaluated by the Lysholm and International Knee Documentation Committee (IKDC) scores. Knee radiographs in standing anteroposterior, standing lateral, and Merchant views were examined for alignment, joint space narrowing, and degenerative knee changes.No major complication like infection, deep venous thrombosis, or neurovascular deficit happened peri-operatively. At the final follow-up, there were no symptoms of instability and no clinical signs of ACL deficiency. Radiographs showed that all fractures healed 3 months post-operative, but at the last follow-up, there was one person with degenerative changes like joint space narrowing in radiographs. Anterior translation of the tibia was 0.47 mm on average (0 to 2.5 mm) compared with the uninjured side. Range-of-motion measurement showed a mean extension deficit of 1.5 (0 to 5) and a mean flexion deficit of 2.7 (0 to 10) compared with the unaffected side. The mean Lysholm score was 96 (85 to 100), and the mean IKDC score was 94 (80 to 100). Overall, the IKDC grade was A (normal) in 24 patients (58%), B (nearly normal) in 8 patients (33%), and C (abnormal) in 1 patient (8%).The present study demonstrated tibial eminence fractures in adults can be effectively treated with arthroscopic suture fixation.


PubMed | Luoyang Orthopedic traumatological Hospital of Henan Province
Type: Journal Article | Journal: Medicine | Year: 2016

Blocked apoptosis and aggressive inflammatory responses occur in fibroblast-like synoviocyte (FLS) of rheumatoid arthritis (RA) patients. Although Brahma-related gene 1 (BRG1) is considered as a tumor suppressor, few research covers its role in RA. This study aims to reveal effects and potential mechanisms of BRG1 in human FLS cell line MH7A.BRG1 expression in MH7A cells was altered by transfection of overexpression vectors or short hairpin RNAs (shRNAs). Cell viability and apoptosis were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry after transfection. Factors involved in inflammation and apoptosis were quantified by qPCR and Western blot. The interaction between BRG1 and p53 was assessed by immunoprecipitation (IP).Results showed that BRG1 overexpression significantly suppressed MH7A cell viability and induced apoptosis (P<0.01), and its knockdown had opposite effects. BRG1 reduced mRNA levels of matrix metallopeptidase 3, TIMP metallopeptidase inhibitor 2, cyclooxygenase 2, and interleukin 6, implying its suppressive effects on inflammation. BRG1 interacted with and promoted p53 (P<0.05). B-cell chronic lymphocytic leukemia/lymphoma 2 was suppressed (P<0.05), while cytochrome c, caspase 3 (CASP3) and CASP9 were activated (P<0.01) by BRG1. However, the regulation on these factors was abrogated by p53 knockdown (P<0.01).These findings suggest that BRG1 may induce apoptosis and suppress inflammation in MH7A cells. Potential functional mechanisms involve the regulation of apoptotic factors by BRG1, which may depend on the recruitment and promotion of p53. This study provides the essential proof for applying BRG1 to the molecular therapy of RA.

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