Li W.,Luoyang Orthopedic Traumatological Hospital |
Cai L.,Luoyang Orthopedic Traumatological Hospital |
Zhang Y.,Chinese Academy of Sciences |
Cui L.,Shanghai JiaoTong University |
Shen G.,Nanjing Medical University
Journal of Orthopaedic Research | Year: 2015
We investigated the feasibility of the intra-articular injection of resveratrol for preventing the progression of existing cartilage degeneration in a mouse model of osteoarthritis (OA). The effects of resveratrol on the expression of silent information regulator 2 type 1 (SIRT1), hypoxia-inducible factor-2α (HIF-2α) and catabolic factors in OA cartilage was explored. OA was induced in the mouse knee via destabilization of the medial meniscus (DMM). Resveratrol was injected weekly into the operated knee beginning 4 weeks after surgery. The OA phenotype was evaluated via histological and immunohistochemical analyses at 8 weeks after DMM. Western blot analysis was performed to identify whether resveratrol modulated the interleukin (IL)-1β-induced expression of HIF-2α in human chondrocytes. Histologically, resveratrol treatment preserved the structural homeostasis of the articular cartilage and the subchondral bone. Following resveratrol injection, the expression of collagen type II was retained, but the expression of inducible nitric oxide synthase and matrix metalloproteinase-13 was reduced in OA cartilage. Moreover, the administration of resveratrol significantly induced the activation of SIRT1 and the inhibition of HIF-2α expression in mouse OA cartilage and in IL-1β-treated human chondrocytes. These findings indicate that the intra-articular injection of resveratrol significantly prevents the destruction of OA cartilage by activating SIRT1 and thereby suppressing the expression of HIF-2α and catabolic factors. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
Influence of percutaneous stimulation of hepatic region with mid-frequency pulse current on the activity of serum GSH-PX, SOD, T-AOC and the content of malondialdehyde in exercise-induced fatigued soldiers
Dai P.-Y.,Luoyang Orthopedic Traumatological Hospital |
Huang C.-L.,150 Hospital of PLA
Medical Journal of Chinese People's Liberation Army | Year: 2014
Objective To explore the influence of percutaneous stimulation of the hepatic region with mid-frequency pulsed current on the serum activity of glutathione peroxidase (GSH-PX), superoxide dismutase (SOD) and total antioxidant capacity and content of malondialdehyde (MDA) in exercise-induced fatigued soldiers. Methods Sixty healthy male recruits without training history were randomly divided into control group and stimulation group (n=30). Subjects in both groups received intensive training for 5 weeks (trained from Monday to Saturday, and rest on Sunday) to establish an exercise-induced fatigue model. The recruits in stimulation group received rehabilitation therapy of percutaneous stimulation of the hepatic region with mid-frequency pulse current (frequency was 1024Hz, dynamic cycle 1s, stimulation time 20min, output intensity ≤80mA) after the training immediately. In every Sunday morning of the 1st, 3rd and 5th week, venous blood samples were obtained from recruits of both groups for determination of the serum activity of GSH-PX, SOD and T-AOC and content of MDA. Results In both groups, the serum activity of GSH-PX and T-AOC on 5th weekend was lower than that of 1st and 3rd weekends, and the serum activity of GSH-PX and T-AOC on 3rd weekend was lower than that of 1st weekend (P<0.05, P<0.01); the serum activity of SOD on the 3rd and 5th weekends was lower than that of 1st weekend (P<0.01), while the SOD activity on the 3rd and the 5th weekend was similar (P>0.05); the serum MDA content on 5th weekend was higher than that of 3rd and 1st weekends, and the content on 3rd weekend was higher than that of 1st weekend (P<0.01). The activity of GSH-PX, SOD and T-AOC increased and the MDA content decreased on 1st, 3rd and 5th weekends in stimulation group when compared with control group (P<0.05, P<0.01). Conclusions The percutaneous stimulation of the hepatic region by mid-frequency pulsed current in exercise-induced fatigued soldiers may improve the activity of antioxidant enzymes in the liver, enhance the function of antioxidant system, promote free radical scavenging, delay the occurrence of and promote the recovery from exercise-induced fatigue.
Dai P.-Y.,Luoyang Orthopedic Traumatological Hospital |
Chang Q.,150 Hospital of PLA
Medical Journal of Chinese People's Liberation Army | Year: 2015
Objective To observe the treatment effect of internal fixation with absorbable suture anchors for calcaneal apophysitis (Sever's disease). Methods The clinical data of 34 Sever's disease patients having received internal fixation with absorbable suture anchors from January 2010 to August 2012 were analyzed retrospectively. Of the 34 patients, 28 were male and 6 were female, aged from 11 to 30 years old (mean 19 years old); 20 with unilateral lesions, 14 with bilateral lesions; 32 suffered inflammatory edema, and 2 with epiphyseal avulsion. All of the patients were diagnosed as suffering from Sever's disease by X-ray combined with clinical symptoms. The sclerotic bone was chiseled off, the attachment site of Achilles tendon was fixed with auxiliary sutures of anchors and then fixed to the bone surface. Different rehabilitation programs were adapted in different periods after operation. The operation time, the site of anchorage and perioperative complications were analyzed, and the therapeutic effects were evaluated by Arner-Lindholm standard. Results All patients were followed up for 2-24 months (mean 9 months). The average operation time for one side was 40.5 minutes. No iatrogenic injury to nerve, vascular or tendon occurred during surgical procedure. No wound infection or foreign-body reaction was found after operation. The therapeutic effects evaluated by Arner-Lindholm standard were excellent in 29 cases, good in 4 and bad in 1 case. Conclusion Absorbable suture anchoring fixation is a reasonable and effective method for the treatment of Sever's disease, and it allows early exercise training for recovery of ankle function. The postoperative functional recovery is found to be satisfactory. © 2015 People's Military Medical Press.
PubMed | Wuhan Puai Hospital and Luoyang Orthopedic Traumatological Hospital
Type: | Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | Year: 2016
Osteosarcoma is the most common primary malignant bone tumor in the pediatric age group, and chemotherapy directed by targeted nanoparticulate drug delivery system represents a promising approach for osteosarcoma treatment recently. Here, we designed and developed a novel DOX-loaded targeted polymeric micelle self-assembled from RGD-terminated poly(ethylene glycol)-block-poly (trimethylene carbonate) (RGD-PEG-PTMC) amphiphilic biodegradable block copolymer, for high-efficiency targeted chemotherapy of osteosarcoma. Notably, the RGD-installed DOX-loaded biodegradable polymeric micelle (RGD-DOX-PM) with drug loading efficiency of 57%-73% displayed a narrow distribution (PDI=0.05-0.12) with average sizes ranging from 46 to 73nm depending on the DOX loading content. The release amount of DOX from RGD-DOX-PM achieved 63% within 60h under physiological condition. Interestingly, MTT assays in MG-63 and MNNG/HOS osteosarcoma cells exhibited that half-maximal inhibitory concentration (IC
Bu B.X.,Zhengzhou University |
Bu B.X.,Luoyang Orthopedic Traumatological Hospital |
Wang M.J.,Luoyang Orthopedic Traumatological Hospital |
Liu W.F.,Jiangsu University |
And 3 more authors.
Orthopaedics and Traumatology: Surgery and Research | Year: 2015
Objective: To evaluate the radiographic outcomes of short-segment posterior instrumentation plus vertebroplasty using injectable calcium sulfate cement (CSC) for thoracolumbar compression fractures. Materials and methods: Twenty-eight patients with a single-level thoracolumbar compression fracture, who underwent short-segment pedicle screw fixation and CSC vertebroplasty, were included in the study. The anterior vertebral body height ratio, local kyphosis angle, and the height of the intervertebral disc adjacent to the fractured vertebra were used to evaluate the radiographic results. Complications including bone nonunion, instrument failure, cement leakage, and disc vacuum formation were also assessed. Results: The patients were followed up for an average of 24.20 ± 5.40 months. The relative preoperative anterior body height was 55.71 ± 15.29%, which improved to 94.93 ± 5.39% immediately after surgery (P < 0.001), and at final follow-up showed a 6.50 ± 3.89% loss of height correction (P < 0.001). The mean preoperative local kyphosis angle was 22.23 ± 5.65°, which corrected to 2.67 ± 4.43° immediately after surgery (P < 0.001), but reverted to 6.71 ± 4.95° at final follow-up, showing a 4.04 ± 1.91° loss of correction (P < 0.001). The mean height of the intervertebral disc proximal to the fractured vertebra was 9.87 ± 0.91 mm before surgery, 12.53 ± 0.98 mm after operation (P < 0.001), and the loss of correction at final follow-up was 2.35 ± 1.15 mm with a significant difference compared to immediate postoperative values (P < 0.001). Bone nonunion occurred in 7 patients, 2 patients had hardware failure, 9 patients had cement leakage, and 10 patients had disc vacuum phenomenon adjacent to the fractured vertebra. Conclusions: The patients who underwent this procedure had a loss of correction of vertebral height and local kyphosis. Complications such as bone nonunion, instrument failure, cement leakage, and disc vacuum may occur. Rapid CSC resorption accounts for these radiographic outcomes and complications. Level of evidence: Level IV, retrospective study. © 2015 Elsevier Masson SAS.
Ma W.L.,Luoyang Orthopedic Traumatological Hospital
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2010
To study the impacts of tetramethylpyrazine (TMP) intervention on hemorheology and blood coagulation before and after free skin flap transplantation, in order to provide new clues of applying Chinese drugs for activating blood circulation and removing stasis in microsurgery. Sixty patients undergoing free skin flap transplantation were randomly and equally assigned to two groups, the treatment group administered with tetramethylpyrazine, the control group with low molecular dextran, both were treated for 7 days. The survival rate of transplanted flap, hemorrheologic parameters and four blood coagulation associated indices (prothrombin time, thrombin time, activated prothrombin time and fibrinogen) were detected before and after treatment. Comparisons of clinical efficacy and hemorrheologic indices showed no significant difference between the two groups (P > 0.05), but TMP showed less impacts on the four blood coagulation associated indices at the 24, 48 and 72 h after operation (P < 0.05). TMP could prevent blood vessel crisis after free skin flap transplantation, so it is valuable in microsurgical clinical application.
Zhang Z.,Luoyang Orthopedic Traumatological Hospital
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2010
To explore the clinical effects of different operative procedures in treatment of upper humerus fracture nonunion. From May 2001 to September 2007, 43 cases of upper humerus fractures nonunion were treated, including 31 males and 12 females with an average age of 37 years (range, 20-57 years). The causes were traffic accident injury in 14 cases, falling injury from height in 11 cases, tumbling injury in 7 cases, heavy pound injury in 6 cases, machine injury in 4 cases, and pathological injury in 1 case. The time from fracture to hospitalization was 10-52 months (23 months on average). After open reduction, patients were treated respectively by bone-graft plus locking compression plate fixation (9 cases), scapula flap rotation displacement plus locking compression plate fixation (15 cases), and scapula flap rotation displacement plus locking compression plate plus tibia bone lamella fixation (19 cases). All incisions healed by first intention. The X-ray films showed good fracture reduction. No symptoms of infection and nerve injury occurred. Forty-three patients were followed up 12 to 25 months with an average of 18 months. All of them achieved radiographic union within 3.0 to 7.5 months (4.9 months on average). According to comprehensive assessing standard of X-ray film and functions of shoulder and elbow, the results were excellent in 21 cases, good in 15 cases, fair in 4 cases, and poor in 3 cases; the excellent and good rate was 83.7%. In the treatment of upper humerus fractures nonunion, locking compression plate can provide stable fixation. It can achieve satisfactory results so long as the right method of bone graft is chosen according to fracture site situation. But for patients undergoing repeated surgery or having nonunion for long times and poor fracture site situation, after open reduction, scapula flap rotation displacement plus locking compression plate plus tibia bone lamella fixation has good outcome.
Yao N.,Luoyang Orthopedic Traumatological Hospital
Chinese Critical Care Medicine | Year: 2012
Objective: To investigate the protective effect of combined pretreatment of edaravone and propofol on cerebral cortex with ischemia/reperfusion (I/R) injury and its therapeutic window. Methods: Sprague Dawley (SD) rat brain cortex cells harvested within 24 hours of birth were cultured in vitro for 7 days. The cells were then divided into blank control group, glutamate injury group, 24-hour drug precondition control group, and 24-, 2-, 0-hour drug precondition groups according to random number table. The nerve cells in each pretreatment group were cultured in medium containing 100 μmol/L of edaravone and 3 mg/L of propofol 24, 2, or 0 hour before glutamate damage (200 μmol/L for 0.5 hour). Nerve cell survival or damage was determined by methyl thiazolyl tetrazolium (MTT), lactate dehydrogenase (LDH) leakage rate, and nerve cell Na+-K+-ATPase activity. The oxidation and anti-oxidation ability of nene cells was observed by determining superoxide dismutase (SOD) activity (xanthine oxidase), malondialdehyde (MDA) content (thiobarbiturie acid). Nerve apoptosis was detected by flow cytometry. Results: Compared with blank control group, in the glutamate injury group, nerve cell survival rate [(62.2 ± 23.4)% vs. (90.5 ± 14.8)%], the activity of SOD (U/ml: 6.864 ± 2.872 vs. 29.569 ± 3.684), Na+-K +-ATPase activity ( U·mg-1·h-1: 0.318 ± 0.146 vs. 0.636 ± 0.168) were significantly decreased, and rate of neuronal apoptosis [(9.4 ± 0.7)% vs. (6.1 ± 0.2)%], the content of MDA (nmol/ml: 0.515 ± 0.101 vs. 0.294 ± 0.105), LDH leakage rate [(41.2 ± 1.6)% vs. (36.8 ±4.6)%] were significantly increased (P<0.05 or P<0.01). Compared with glutamate injury group, the cell survival rate and the activity of SOD and Na+-K+-ATPase were significantly increased in the drug pretreatment groups, and apoptosis rate, MDA content, and LDH leakage rate were significantly decreased with time-department, and effect in the 24-hour pretreatment group was most significant [survival rate of cell: (89.2 ± 30.3)%vs. (62.2 ± 23.4)%, SOD activity (U/ml): 17.780 ± 4.514 vs. 6.864 ± 2.872, Na+-K+-ATPase activity (U·mg -1·h-1): 0.541 ± 0.052 vs. 0.318 ± 0.146, the rate of cell apoptosis: (6.7 ± 0.4)% vs. (9.4 ± 0.7)%, the content of MDA (nmol/ml): 0.319 ± 0.101 vs. 0.515 ± 0.101, LDH leakage rate: (37.2 ± 1.4)% vs. (41.2 ± 1.6)%, all P<0.01]. Conclusion: The synergistic protective effect of pretreatment with edaravone combined with propofol on neonatal rat brain cortex cells with I/R injury in vitro was evident ; and 24-hour pretreatment is the best time window of protection for the cerebral neurons.
Zhang X.,Luoyang Orthopedic Traumatological Hospital |
Zhu Y.,Luoyang Orthopedic Traumatological Hospital |
Chen X.,Luoyang Orthopedic Traumatological Hospital |
Zhang Y.,Chinese Academy of Sciences |
And 5 more authors.
International Immunopharmacology | Year: 2014
Osteoarthritis (OA), characterized by progressive destruction of articular cartilage, is the most common form of human arthritis and a major concern for aging societies worldwide. In OA, pro-inflammatory cytokines such as interleukin (IL)-1β and tumor necrosis factor (TNF)-α can trigger the caspase cascade to promote apoptosis and activate NF-κB to induce catabolic factors in chondrocytes. Here, the anti-apoptotic and anti-catabolic effects of baicalein on human OA chondrocytes treated by a mixture of IL-1β and TNF-α (IT) were investigated in vitro. In cultured chondrocytes, baicalein pretreatment attenuated apoptosis in a concentration-dependent manner in response to IT stimulation. Mechanistically, the anti-apoptotic effects of baicalein result from inhibition of nitric oxide production and downstream caspase signaling pathway. Moreover, administration of baicalein significantly reduced matrix metalloproteinase (MMP) 3 and MMP13 secretion in chondrocytes stimulated with IT. The anti-catabolic effects of baicalein were further demonstrated by the recovery of the glycosaminoglycan and type II collagen (COLII) deposition by histological analysis in IT-treated mouse articular cartilage explants. These findings suggest that baicalein might be a promising novel therapeutic agent for OA by virtue of its suppression of apoptosis and MMP secretion in OA chondrocytes. © 2014 Elsevier B.V.
PubMed | Luoyang Orthopedic Traumatological Hospital
Type: Journal Article | Journal: Medicine | Year: 2016
It was a retrospective case-control study. The aim of this study was to explore the clinical efficacy and complication of treatment using a modified Kirschner wire tension band (MKTB) or a cannulated screw tension band (CSTB) in transverse patellar fractures.In total, 55 patients with transverse patellar fractures were retrospectively reviewed and divided into 2 groups according to the surgical technique: 29 patients were in the MKTB group and 26 patients in the CSTB group. B[Latin Small Letter o with Caron]stmans clinical grading scale, including range of movement (ROM), pain, ability to work, atrophy of quadriceps femoris, assistance in walking, effusion, giving way, and stair-climbing, was used to evaluate the clinical results. Complications including painful hardware, implant loosening or breakage, and bone nonunion were also assessed.Both groups were evaluated at the final follow-up before removing implant in the MKTB group. The B[Latin Small Letter o with Caron]stmans score of ROM, pain, atrophy of quadriceps femoris, and effusion were all higher in the CSTB group than in the MKTB group (P<0.05). Twelve patients in the MKTB group underwent implant removal, and the score of ROM, pain, and effusion were higher than before removing implant (P<0.05), but there was no difference compared to the CSTB group (P>0.05). Seventeen patients achieved excellent results, 9 had good results, and 3 reported fair results in the MKTB group; the CSTB group had excellent results in 22 patients and good results in 4 patients, showing a significant difference in the excellent rate between the 2 groups (P=0.021). Total B[Latin Small Letter o with Caron]stman scores in the MKTB and CSTB groups (26.964.47 and 29.421.47, respectively) were significantly different (P=0.01). Total scores in the MKTB group after removing implant were higher than those before removing implant (P=0.001), and similar to those in the CSTB group (P=0.224). Eleven patients in the MKTB group reported painful hardware, including 4 cases of implant loosening.CSTB achieves better clinical results than MKTB, meanwhile avoiding the problems of painful hardware and implant loosening. Functional limitation caused by hardware pain was commonly seen in the MKTB group, and removing implant after fracture healing improved knee function.