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Dai P.-Y.,Luoyang Orthopedic Traumatological Hospital | Huang C.-L.,150 Hospital of PLA
Medical Journal of Chinese People's Liberation Army | Year: 2014

Objective To explore the influence of percutaneous stimulation of the hepatic region with mid-frequency pulsed current on the serum activity of glutathione peroxidase (GSH-PX), superoxide dismutase (SOD) and total antioxidant capacity and content of malondialdehyde (MDA) in exercise-induced fatigued soldiers. Methods Sixty healthy male recruits without training history were randomly divided into control group and stimulation group (n=30). Subjects in both groups received intensive training for 5 weeks (trained from Monday to Saturday, and rest on Sunday) to establish an exercise-induced fatigue model. The recruits in stimulation group received rehabilitation therapy of percutaneous stimulation of the hepatic region with mid-frequency pulse current (frequency was 1024Hz, dynamic cycle 1s, stimulation time 20min, output intensity ≤80mA) after the training immediately. In every Sunday morning of the 1st, 3rd and 5th week, venous blood samples were obtained from recruits of both groups for determination of the serum activity of GSH-PX, SOD and T-AOC and content of MDA. Results In both groups, the serum activity of GSH-PX and T-AOC on 5th weekend was lower than that of 1st and 3rd weekends, and the serum activity of GSH-PX and T-AOC on 3rd weekend was lower than that of 1st weekend (P<0.05, P<0.01); the serum activity of SOD on the 3rd and 5th weekends was lower than that of 1st weekend (P<0.01), while the SOD activity on the 3rd and the 5th weekend was similar (P>0.05); the serum MDA content on 5th weekend was higher than that of 3rd and 1st weekends, and the content on 3rd weekend was higher than that of 1st weekend (P<0.01). The activity of GSH-PX, SOD and T-AOC increased and the MDA content decreased on 1st, 3rd and 5th weekends in stimulation group when compared with control group (P<0.05, P<0.01). Conclusions The percutaneous stimulation of the hepatic region by mid-frequency pulsed current in exercise-induced fatigued soldiers may improve the activity of antioxidant enzymes in the liver, enhance the function of antioxidant system, promote free radical scavenging, delay the occurrence of and promote the recovery from exercise-induced fatigue. Source

Objective: To investigate the protective effect of combined pretreatment of edaravone and propofol on cerebral cortex with ischemia/reperfusion (I/R) injury and its therapeutic window. Methods: Sprague Dawley (SD) rat brain cortex cells harvested within 24 hours of birth were cultured in vitro for 7 days. The cells were then divided into blank control group, glutamate injury group, 24-hour drug precondition control group, and 24-, 2-, 0-hour drug precondition groups according to random number table. The nerve cells in each pretreatment group were cultured in medium containing 100 μmol/L of edaravone and 3 mg/L of propofol 24, 2, or 0 hour before glutamate damage (200 μmol/L for 0.5 hour). Nerve cell survival or damage was determined by methyl thiazolyl tetrazolium (MTT), lactate dehydrogenase (LDH) leakage rate, and nerve cell Na+-K+-ATPase activity. The oxidation and anti-oxidation ability of nene cells was observed by determining superoxide dismutase (SOD) activity (xanthine oxidase), malondialdehyde (MDA) content (thiobarbiturie acid). Nerve apoptosis was detected by flow cytometry. Results: Compared with blank control group, in the glutamate injury group, nerve cell survival rate [(62.2 ± 23.4)% vs. (90.5 ± 14.8)%], the activity of SOD (U/ml: 6.864 ± 2.872 vs. 29.569 ± 3.684), Na+-K +-ATPase activity ( U·mg-1·h-1: 0.318 ± 0.146 vs. 0.636 ± 0.168) were significantly decreased, and rate of neuronal apoptosis [(9.4 ± 0.7)% vs. (6.1 ± 0.2)%], the content of MDA (nmol/ml: 0.515 ± 0.101 vs. 0.294 ± 0.105), LDH leakage rate [(41.2 ± 1.6)% vs. (36.8 ±4.6)%] were significantly increased (P<0.05 or P<0.01). Compared with glutamate injury group, the cell survival rate and the activity of SOD and Na+-K+-ATPase were significantly increased in the drug pretreatment groups, and apoptosis rate, MDA content, and LDH leakage rate were significantly decreased with time-department, and effect in the 24-hour pretreatment group was most significant [survival rate of cell: (89.2 ± 30.3)%vs. (62.2 ± 23.4)%, SOD activity (U/ml): 17.780 ± 4.514 vs. 6.864 ± 2.872, Na+-K+-ATPase activity (U·mg -1·h-1): 0.541 ± 0.052 vs. 0.318 ± 0.146, the rate of cell apoptosis: (6.7 ± 0.4)% vs. (9.4 ± 0.7)%, the content of MDA (nmol/ml): 0.319 ± 0.101 vs. 0.515 ± 0.101, LDH leakage rate: (37.2 ± 1.4)% vs. (41.2 ± 1.6)%, all P<0.01]. Conclusion: The synergistic protective effect of pretreatment with edaravone combined with propofol on neonatal rat brain cortex cells with I/R injury in vitro was evident ; and 24-hour pretreatment is the best time window of protection for the cerebral neurons. Source

Dai P.-Y.,Luoyang Orthopedic Traumatological Hospital | Chang Q.,150 Hospital of PLA
Medical Journal of Chinese People's Liberation Army | Year: 2015

Objective To observe the treatment effect of internal fixation with absorbable suture anchors for calcaneal apophysitis (Sever's disease). Methods The clinical data of 34 Sever's disease patients having received internal fixation with absorbable suture anchors from January 2010 to August 2012 were analyzed retrospectively. Of the 34 patients, 28 were male and 6 were female, aged from 11 to 30 years old (mean 19 years old); 20 with unilateral lesions, 14 with bilateral lesions; 32 suffered inflammatory edema, and 2 with epiphyseal avulsion. All of the patients were diagnosed as suffering from Sever's disease by X-ray combined with clinical symptoms. The sclerotic bone was chiseled off, the attachment site of Achilles tendon was fixed with auxiliary sutures of anchors and then fixed to the bone surface. Different rehabilitation programs were adapted in different periods after operation. The operation time, the site of anchorage and perioperative complications were analyzed, and the therapeutic effects were evaluated by Arner-Lindholm standard. Results All patients were followed up for 2-24 months (mean 9 months). The average operation time for one side was 40.5 minutes. No iatrogenic injury to nerve, vascular or tendon occurred during surgical procedure. No wound infection or foreign-body reaction was found after operation. The therapeutic effects evaluated by Arner-Lindholm standard were excellent in 29 cases, good in 4 and bad in 1 case. Conclusion Absorbable suture anchoring fixation is a reasonable and effective method for the treatment of Sever's disease, and it allows early exercise training for recovery of ankle function. The postoperative functional recovery is found to be satisfactory. © 2015 People's Military Medical Press. Source

Teng J.,The Key Laboratory of Cardiovascular Remodeling and Function Research | Teng J.,Shandong University | Wei Y.,Henan Province Peoples Hospital | Guo Z.,Luoyang Orthopedic Traumatological Hospital | And 2 more authors.
International Journal of Environmental Research and Public Health | Year: 2015

In this study, a multiple linear regression model to evaluate the risk of morbidity and mortality of ischemic cardiovascular disease is demonstrated. In this model, predictor variables are selected from physiological chemicals in a blood test of the subjects. Meanwhile, the calculated risk score is selected as a response variable. Four major latent variables including hepatic, nephric, metabolic, and BMI (Body Mass Index) are revealed by performing statistical and principal component analysis for the collected survey data. The analyzed result also shows that the cardiac disorder is correlated with symptoms of abnormal BMI, hepatic disorder, nephric disorder, and metabolic disorder. Thus, the risk of morbidity and mortality of ischemic cardiovascular disease can be assessed from the proposed multiple regression model. © 2015 by the authors; licensee MDPI, Basel, Switzerland. Source

Li W.,Luoyang Orthopedic Traumatological Hospital | Cai L.,Luoyang Orthopedic Traumatological Hospital | Zhang Y.,Chinese Academy of Sciences | Cui L.,Shanghai JiaoTong University | Shen G.,Nanjing Medical University
Journal of Orthopaedic Research | Year: 2015

We investigated the feasibility of the intra-articular injection of resveratrol for preventing the progression of existing cartilage degeneration in a mouse model of osteoarthritis (OA). The effects of resveratrol on the expression of silent information regulator 2 type 1 (SIRT1), hypoxia-inducible factor-2α (HIF-2α) and catabolic factors in OA cartilage was explored. OA was induced in the mouse knee via destabilization of the medial meniscus (DMM). Resveratrol was injected weekly into the operated knee beginning 4 weeks after surgery. The OA phenotype was evaluated via histological and immunohistochemical analyses at 8 weeks after DMM. Western blot analysis was performed to identify whether resveratrol modulated the interleukin (IL)-1β-induced expression of HIF-2α in human chondrocytes. Histologically, resveratrol treatment preserved the structural homeostasis of the articular cartilage and the subchondral bone. Following resveratrol injection, the expression of collagen type II was retained, but the expression of inducible nitric oxide synthase and matrix metalloproteinase-13 was reduced in OA cartilage. Moreover, the administration of resveratrol significantly induced the activation of SIRT1 and the inhibition of HIF-2α expression in mouse OA cartilage and in IL-1β-treated human chondrocytes. These findings indicate that the intra-articular injection of resveratrol significantly prevents the destruction of OA cartilage by activating SIRT1 and thereby suppressing the expression of HIF-2α and catabolic factors. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. Source

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