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Zhao A.-Q.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Zhao J.-H.,Henan University of Science and Technology | Zhang S.-Q.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Pan Y.-Y.,Henan University of Science and Technology | Huo X.-L.,Henan University of Science and Technology
Journal of Pharmaceutical and Biomedical Analysis | Year: 2016

A rapid, sensitive and selective ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the determination and pharmacokinetic investigation of parthenolide in rat plasma. Sample preparation was accomplished through a simple one-step deproteinization procedure with 0.2. mL of acetonitrile containing 30 ng/mL of pirfenidone (IS), and to a 0.1. mL plasma sample. Plasma samples were separated by UPLC on an Acquity UPLC BEH C18 column using a mobile phase consisting of acetonitrile-0.1% formic acid in water with gradient elution. The total run time was 3.0. min and the elution of parthenolide was at 1.33. min. The detection was performed on a triple quadrupole tandem mass spectrometer in the multiple reaction-monitoring (MRM) mode using the respective transitions m/. z 249.2. →. 231.1 for parthenolide and m/. z 186.2. →. 92.1 for pirfenidone (IS), respectively. The calibration curve was linear over the range of 2.0-500. ng/mL with a lower limit of quantitation (LLOQ) of 2.0. ng/mL. Mean recovery of parthenolide in plasma was in the range of 78.2-86.6%. Intra-day and inter-day precision were both <8.3%. This method was successfully applied in pharmacokinetic study after oral and intravenous administration of parthenolide in rats. © 2015 Elsevier B.V.


Feng W.-H.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Zhang H.-H.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Zhang Y.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Sun M.,Henan University of Science and Technology | Niu J.-L.,Henan University of Science and Technology
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2015

In this study, a simple, sensitive, and robust analytical method based on ultra-performance liquid chromatography (UPLC) has been developed for the determination of galangin in rat plasma using diazepam as internal standard (IS). After sample preparation by a simple liquid-liquid extraction, chromatography was performed on an Acquity UPLC BEH C18 column (2.1. mm. ×. 50. mm, 1.7. μm particle size) and ultraviolet detection set at a wavelength of 360. nm. The method was linear over the concentration range 10-1000. ng/mL with a lower limit of quantification (LLOQ) of 10. ng/mL. Inter- and intra-day precision (RSD %) were all within 9.5% and the accuracy (RE %) was equal or lower than 8.9%. Recoveries of galangin and IS were more than 78.3%. Stability studies showed that galangin was stable under a variety of storage conditions. The method was successfully applied to a pharmacokinetic study involving oral administration of galangin to rats. © 2015 Elsevier B.V.


Jin G.,Luoyang Orthopedic Traumatological Hospital of Henan Province
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2010

To study the treatment method and effect of abduction and lateral rotation limitation of the shoulder in obstetric brachial plexus palsy (OBPP). From February 2005 to August 2008, 11 patients with abduction and lateral rotation limitation of the shoulder in OBPP were treated with dissection of the origin of subscapular muscle, transfer of the tendons of latissimus dorsi and teres major muscle to the tendons of supraspinous and infraspinous muscles. Among them, there were 6 males and 5 females with a mean age of 6 years (1-15 years). The main clinical manifestations showed adduction, internal rotation contracture deformity of shoulder, limited active and passive external rotation and severely restricted active abduction of shoulder. The passive abduction was more than 90 degrees. According to Gilbert grading, there were 7 cases of grade 1 and 4 cases of grade 2. Based on Mallet score systems, the scores were 5 points in 3 cases, 6 points in 3 cases, and 7 points in 5 cases. The muscle strength of deltoid, supraspinatus, infraspinatus, teres major muscle and latissimus dorsi all reached 3-4 grades. One patient developed postoperative hematoma, wound healed after symptomatic management. Other patients achieved incision healing by first intention. All patients were followed up for 12 to 37 months (17 months on average). The active abduction and external rotation of the shoulder joints recovered obviously. The Gilbert grading were grade 2 in 1 case, grade 3 in 1 case, and grade 4 in 9 cases; the Mallet scores were 10 points in 1 case, 11 points in 2 cases, 12 points in 4 cases, 13 points in 3 cases, and 14 points in 1 case; showing significant differences when compared with those before operation (P < 0.01). The muscle strength of deltoid, supraspinatus, infraspinatus, teres major muscle and latissimus dorsi increased to 4-5 grades. The dissection of the origin of subscapular muscle, transfer of the tendons of latissimus dorsi and teres major muscle to the tendons of supraspinous and infraspinous muscles can resolve shoulder adduction, internal rotation contracture, and can enhance abduction, external rotation strength. It is an effective operation for abduction and lateral rotation limitation of the shoulder in OBPP.


Lv S.-F.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Wang X.-H.,Henan University of Science and Technology | Li H.-W.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Zhang X.-L.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Wang B.,Luoyang Orthopedic Traumatological Hospital of Henan Province
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2015

An ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine liensinine in rat plasma using carbamazepine as the internal standard (IS). Sample preparation was accomplished through a protein precipitation procedure with acetonitrile to 0.1ml plasma sample. The analyte and IS were separated on an Acquity UPLC BEH C18 column (2.1mm×50mm, 1.7μm) with the mobile phase of acetonitrile and 0.1% formic acid in water with gradient elution at a flow rate of 0.40ml/min. The injection volume was 6μl. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization (ESI) by multiple reactions monitoring (MRM) of the transitions at m/z 611.6→206.2 for liensinine and m/z 237.1→194.2 for IS. The linearity of this method was found to be within the concentration range of 10-1000ng/ml with a lower limit of quantification of 10ng/ml. Only 3.0min was needed for an analytical run. The matrix effect was 93.8-107.4% for liensinine. The intra- and inter-day precision (RSD %) were less than 9.9% and accuracy (RE %) was within ±10.5%. The recovery ranged from 76.2 to 86.8%. Liensinine was sufficiently stable under all relevant analytical conditions. The method was also successfully applied to the pharmacokinetic study of liensinine in rats. The pharmacokinetic parameters were demonstrated as followed: t1/2 was 8.2±3.3h, Cmax was 668.4±156.9ng/ml, and AUC0→∞ was 1802.9±466.4ng/mlh. © 2015 Elsevier B.V.


Lv S.-F.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Wang X.-H.,Henan University of Science and Technology | Wang B.,Luoyang Orthopedic Traumatological Hospital of Henan Province | Li H.-W.,Luoyang Orthopedic Traumatological Hospital of Henan Province | And 4 more authors.
Analytical Methods | Year: 2015

In this study, a simple, sensitive, and robust analytical method based on ultra-performance liquid chromatography (UPLC) has been developed for the determination of kaempferide in rat plasma using diazepam as the internal standard (IS). After sample preparation by a simple liquid-liquid extraction, chromatography was performed on an Acquity UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm particle size) and ultraviolet detection was set at a wavelength of 366 nm. The analysis was achieved with a mobile phase of solvent A (water) : solvent B (acetonitrile) : solvent C (0.1% trifluoroacetic acid) = 35 : 45 : 20. The flow rate was 0.30 mL min-1 and total run time was 2.0 minutes. The method was linear over the concentration range of 25-1000 ng mL-1 with a lower limit of quantification (LLOQ) of 25 ng mL-1. Inter- and intra-day precisions (RSD%) were all within 10.2% and the accuracy (RE%) was equal to or lower than 9.3%. Recoveries of kaempferide and IS were more than 78.4%. Stability studies showed that kaempferide was stable under a variety of storage conditions. The method was successfully applied to a pharmacokinetic study, involving the intravenous administration of kaempferide to rats. © 2015 The Royal Society of Chemistry.

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