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Luoyang, China

Hao X.-Q.,Henan University of Science and Technology | Hao X.-Q.,Chinese Institute of Materia Medica | Kong T.,Henan University of Science and Technology | Zhang S.-Y.,Luoyang Central Hospital | Zhao Z.-S.,Henan University of Science and Technology
Experimental and Toxicologic Pathology | Year: 2013

Prenatal exposure to LPS(lipopolysaccharide) results in renal damage in offspring rats, but the mechanism is unknown. The present study was to explore the role of angiotensin II and inflammation in the development of renal damage induced by prenatal exposure to LPS. The pregnant rats were randomly divided into two groups, i.e., control group, LPS group. The rats in the two groups were administered intraperitoneally with vehicle or 0.79mg/kg LPS on 8th, 10th and 12th day during gestation. The mRNA expression of angiotensinogen, renin, AT1-R, AT2-R, TNF-α and IL-6 in embryos were assessed. Renal Ang II-positive cells, monocytes/macrophages, lymphocytes, collagen I and TUNEL-positive cells were identified by immunohistochemical staining in newborn and 7-week-old offspring rats. The number of glomeruli and creatinine clearance rate were determined in offspring at 7 weeks of age. The results showed that prenatal LPS decreased AT2-R mRNA expression but increased TNF-α and IL-6 mRNA expression in embryos. Prenatal LPS decreased renal angiotensin II-positive cells in newborn offspring rats, while these increased in 7-week-old offspring rats. Prenatal LPS decreased glomerular number and creatinine clearance rate but increased renal infiltrating monocytes/macrophages and lymphocytes at 7 weeks of age. Prenatal LPS also increased TUNEL-positive cells and collagen I expressions in newborn rats and 7-week-old offspring rats. Conclusion: Alteration of embryonic AT2-R and inflammatory cytokines gene expression induced by prenatal exposure to lipopolysaccharide affects renal development. © 2012 Elsevier GmbH. Source

Feng Y.,Luoyang Central Hospital
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2011

To analyze the expression of deleted in liver cancer 1 (DLC1) and phosphorelated focal adhesion kinase (p-FAK) in breast cancer tissue to further understand the molecular mechanisms of the carcinogenesis and metastasis of breast cancer. Immunohistochemistry was employed to determine the protein level of DLC1 and p-FAK in 61 breast cancer, 30 benign breast disease and the adjacent normal breast tissues. The positivity rates of DLC1 differed significantly between breast cancer, benign and normal tissues (34.43%, 80.00% and 76.67%, respectively, P<0.001). The positivity rates of p-FAK in the 3 tissues were 77.05%, 33.33% and 26.67%, also showing significant differences (P<0.001). The aberrant expression of DLC1 showed an inverse correlation to p-FAK (κ=-0.4591). Both DLC1 and p-FAK were closely correlated to the carcinogenesis, clinical stage, PR and lymphatic metastasis of breast cancer (P<0.05), but not to the patients age, pathological subtype, familial history, ER or CerbB-2 (P>0.05). The abnormal expression of DLC1 and p-FAK might participate in the carcinogenesis, progression, and metastasis of breast cancer. The role of DLC1 and p-FAK might be related to the regulation of progestone. DLC1 and p-FAK may serve as candidate markers for early diagnosis, prognostic evaluation and target treatment of breast cancer. Source

Li T.-F.,Zhengzhou University | Shui S.-F.,Zhengzhou University | Han X.-W.,Zhengzhou University | Yan L.,Zhengzhou University | And 9 more authors.
PLoS ONE | Year: 2015

Background The Solitaire AB stent is one of many assistant stents used for treating wide-necked cerebral aneurysm, and has been used since 2003. However, large sample studies on its safety and effectiveness are lacking. The objective of this study was to evaluate the effectiveness and safety of the Solitaire AB stent in the coil embolization of wide-necked cerebral aneurysms. Methods Retrospective review of the clinical and image data of 116 patients with wide-necked cerebral aneurysms who had been enrolled at six interventional neuroradiology centers from February 2010 to February 2014 and had been treated by coil embolization; in total, 120 Solitaire AB stents were used. The degree of aneurysm occlusion was examined using digital subtraction angiography (DSA) immediately after the procedure and during follow-up, and was graded using the modified Raymond classification. We also observed complications to evaluate the safety and effectiveness of this therapy. Results The 120 Solitaire AB stents (4 mm × 15 mm, four stents; 4 mm × 20 mm, 16 stents; 6 mm × 20 mm, 36 stents; 6 mm × 30 mm, 64 stents) were inserted to treat 120 wide-necked cerebral aneurysms. All stents were inserted successfully. DSA immediately post-surgery revealed 55 cases of complete occlusion, 59 cases of neck remnant, and six cases of aneurysm remnant. Perioperatively, there were four cases of hemorrhage and four cases of stent thrombosis. The follow-up spanned 3-37 months; of 92 patients examined by DSA at the 6- month follow up, 12 had disease recurrence. Conclusions The Solitaire AB stent is effective with a good technical success rate and short-term effect for assisting coil embolization of wide-necked cerebral aneurysms. © 2015 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source

Li X.,University of Minnesota | Li X.,Zhengzhou University | Wen W.,University of Minnesota | Liu K.,University of Minnesota | And 11 more authors.
Journal of Biological Chemistry | Year: 2011

p21-activated kinase (PAK) 2, a member of the PAK family of serine/threonine protein kinases, plays an important role in physiological processes such as motility, survival, mitosis, and apoptosis. However, the role of PAK2 in resistance to chemotherapy is unclear. Here we report that PAK2 is highly expressed in human breast cancer cell lines and human breast invasive carcinoma tissue compared with a human non-tumorigenic mammary epithelial cell line and adjacent normal breast tissue, respectively. Interestingly, we found that PAK2 can bind with caspase-7 and phosphorylate caspase-7 at the Ser-30, Thr-173, and Ser-239 sites. Functionally, the phosphorylation of caspase-7 decreases its activity, thereby inhibiting cellular apoptosis. Our data indicate that highly expressed PAK2 mediates chemotherapeutic resistance in human breast invasive ductal carcinoma by negatively regulating caspase-7 activity. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc. Source

Duan X.,Peking Union Medical College | Ji B.,Chinese Academy of Sciences | Liu G.,Chinese Academy of Sciences | Li Q.,Chinese Academy of Sciences | And 4 more authors.
Perfusion | Year: 2011

Background: It is well documented that lower pump flow during clinical cardiopulmonary bypass (CPB) procedures may be significantly detrimental to tissues and organs.The major cause of hypoperfusion is due to shunting flow existing in the closed CPB circuits.The purpose of this investigation was to evaluate various shunting flow conditions in a simulated adult CPB model under normothermia. Materials and Methods: The pseudo adult patient undergoing CPB was established with an extracorporeal circuit.The pre-oxygenator flow, post-filter flow and purge line pressure were recorded. The temperature of the circuit was kept around 36°C using a heat-cooIer. The pseudo patient was subjected to five pump flow rates ranging from 3000 to 5000 ml/min (500 ml/min increments), and post-filter pressure was varied from 120-220 mmHg (20 mmHg increments) using a single, static roller pump. Four different shunting states were measured with two flowmeters. Results: When the pump flow was constant, the percentage of shunting flow increased as the post-filter pressure increased in State I. However, when the post-filter pressure was constant, the trend of the shunting flow was the opposite. Purge line pressure increased with the increase of the post-filter pressure at a constant pump flow rate in State I. The shunting flow for State 2 was the largest of the four states.The shunting flow for State 3 was similar to State 4. Conclusion: During different states of shunt opening, varying degrees of blood flow were diverted away from the arterial line. Shunting of blood flow may, therefore, result in hypoperfusion in the patient. © The Author(s) 2011. Source

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